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The map label for this gene is lysS [H]

Identifier: 78223563

GI number: 78223563

Start: 2668026

End: 2669501

Strand: Reverse

Name: lysS [H]

Synonym: Gmet_2360

Alternate gene names: 78223563

Gene position: 2669501-2668026 (Counterclockwise)

Preceding gene: 78223564

Following gene: 78223562

Centisome position: 66.78

GC content: 59.69

Gene sequence:

>1476_bases
ATGGAAGAACTGAGCGAATTGCTGCTTCAAAGGCGGCGCAAGGTCGATGCCCTCTGGGAGGCAGGGATCAACCCCTACCC
CAATGACTATAAACCCCGGCACACATCGGCGGACGTGGTGGCTGCGTATGGAAAGTGCGAGATTATAGAAGAGGGGGCAG
ACCCCTTTGTGGTGGCCGGTCGGATCATTGCTCGCCGCTCCTTCGGCAAGGCCGCCTTCGTCCAGCTCCAGGACCGCAAG
GGGCGCATACAGCTCTACGTGCGCAAGGACACTCTCGGTGACGAGGCCTATGAAGCCTTTGAGTCCTTCGATATCGGCGA
CATTATCGGCGCCACCGGCACCCCCTTCCGCACCAAGACCGGCGAGCTCTCCCTTAACGCGAGCGAGGTCAGGCTCCTCA
CCAAATCGTTCCTTCCTCTTCCGGAGAAATTCCACGGTCTCACCGATGTCGAGACCCGATATCGCCAGCGCTACGTTGAC
CTGATCGTGAATCCCGAGGTGCGGGAGGTCTTCATCAAGCGCTCCCGGATCGTCAACCTGATCCGCGAATTCATGGTGCG
TCACGACTTCCTCGAAGTGGAAACGCCCATGATGCAGCCGATTCCCGGAGGAGCCACGGCCCGTCCCTTCGTTACCCACC
ACAACGCCCTCGACATGGAGCTTTTTCTCCGCATCGCGCCGGAGCTCTATCTCAAGCGTCTGGTGGTCGGAGGGTTCGAA
CGGGTCTTCGAGATTAACCGCAACTTCCGCAATGAGGGAATTTCGGTCCGTCACAACCCGGAGTTTACCATGATGGAGTT
CTACCAGGCCTACGCCACCTTCGAGGACCTGATGGATTTCACCGAGGAGCTTCTCTGTCACGTGACCCAGGAGGTGCTCG
GAACCCTCGACTTCACCTATCAGGGGGTGGAGATCAGCTTCCAGCGTCCCTGGAAGCGGCTTACCGTGAAGGAGGCGATC
CTCGAGTACGGCGACATCGACGCAAAATCCCTCGAAGATCGCGACCTGGCCTATGCCTACGCCCGGAGCATCGGGCTCGA
CCTGCCGACCGACGTGGGCTACGGCAAGCTTATCACCGAGATTTTCGAGGAGGTGGCGGAGGTAAAGCTGATCCAGCCCA
CCTTCATCACCGCGTATCCCACGGAGGTGTCGCCCCTTTCACGCAAGAGCGACAAGGATCCCGATATCGTCGACCGTTTC
GAGTTCTTTTGCGCCGGCCGCGAGATGGCCAATGCCTTCTCGGAGCTCAACGACCCCGTGGACCAGAAGGAGCGCTTCCT
GGGTCAGGTTGCCCAGAAAGCCAAGGGTGACGAAGAGGCCCACTACATGGACGAGGACTACATACGCGCCCTTGAGTACG
GGATGCCTCCGACGGCCGGAGAAGGGATCGGCATCGACCGCCTCGTCATGCTCCTGACCGACTCCCCGTCCATCCGGGAC
GTCATTCTCTTCCCGCAACTTCGCAAGGAGAAATAA

Upstream 100 bases:

>100_bases
AAAGTTCCCGGTTTTATCTTGACTTGGCTTTGCCCGTGGGCTAATTTGCGGGTTTGTTAAACCGGTCGAAACCGGCACAG
AGAAAGGGATTACACGTCAC

Downstream 100 bases:

>100_bases
CGGGATTACGTAAGCAGATCGAGAATCAGGGGCCTTCTGCGGTCCCTGATTTCCGGTCCACAGTCCCCGGATCCAAAATG
TCCTACGAACTCTTTATTGG

Product: lysyl-tRNA synthetase, class-2

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYKPRHTSADVVAAYGKCEIIEEGADPFVVAGRIIARRSFGKAAFVQLQDRK
GRIQLYVRKDTLGDEAYEAFESFDIGDIIGATGTPFRTKTGELSLNASEVRLLTKSFLPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFIKRSRIVNLIREFMVRHDFLEVETPMMQPIPGGATARPFVTHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVTQEVLGTLDFTYQGVEISFQRPWKRLTVKEAI
LEYGDIDAKSLEDRDLAYAYARSIGLDLPTDVGYGKLITEIFEEVAEVKLIQPTFITAYPTEVSPLSRKSDKDPDIVDRF
EFFCAGREMANAFSELNDPVDQKERFLGQVAQKAKGDEEAHYMDEDYIRALEYGMPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK

Sequences:

>Translated_491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYKPRHTSADVVAAYGKCEIIEEGADPFVVAGRIIARRSFGKAAFVQLQDRK
GRIQLYVRKDTLGDEAYEAFESFDIGDIIGATGTPFRTKTGELSLNASEVRLLTKSFLPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFIKRSRIVNLIREFMVRHDFLEVETPMMQPIPGGATARPFVTHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVTQEVLGTLDFTYQGVEISFQRPWKRLTVKEAI
LEYGDIDAKSLEDRDLAYAYARSIGLDLPTDVGYGKLITEIFEEVAEVKLIQPTFITAYPTEVSPLSRKSDKDPDIVDRF
EFFCAGREMANAFSELNDPVDQKERFLGQVAQKAKGDEEAHYMDEDYIRALEYGMPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK
>Mature_491_residues
MEELSELLLQRRRKVDALWEAGINPYPNDYKPRHTSADVVAAYGKCEIIEEGADPFVVAGRIIARRSFGKAAFVQLQDRK
GRIQLYVRKDTLGDEAYEAFESFDIGDIIGATGTPFRTKTGELSLNASEVRLLTKSFLPLPEKFHGLTDVETRYRQRYVD
LIVNPEVREVFIKRSRIVNLIREFMVRHDFLEVETPMMQPIPGGATARPFVTHHNALDMELFLRIAPELYLKRLVVGGFE
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVTQEVLGTLDFTYQGVEISFQRPWKRLTVKEAI
LEYGDIDAKSLEDRDLAYAYARSIGLDLPTDVGYGKLITEIFEEVAEVKLIQPTFITAYPTEVSPLSRKSDKDPDIVDRF
EFFCAGREMANAFSELNDPVDQKERFLGQVAQKAKGDEEAHYMDEDYIRALEYGMPPTAGEGIGIDRLVMLLTDSPSIRD
VILFPQLRKEK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=41.2825651302605, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=41.2825651302605, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI45439306, Length=482, Percent_Identity=23.0290456431535, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=493, Percent_Identity=59.2292089249493, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=58.0121703853955, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=32.9230769230769, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788173, Length=253, Percent_Identity=29.2490118577075, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=41.4342629482072, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=41.4342629482072, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71994340, Length=497, Percent_Identity=41.6498993963783, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17551876, Length=474, Percent_Identity=23.2067510548523, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=261, Percent_Identity=25.6704980842912, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=510, Percent_Identity=41.9607843137255, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324256, Length=476, Percent_Identity=30.4621848739496, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=359, Percent_Identity=25.9052924791086, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=450, Percent_Identity=24.8888888888889, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=192, Percent_Identity=29.6875, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640849, Length=489, Percent_Identity=42.1267893660532, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=489, Percent_Identity=42.1267893660532, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=266, Percent_Identity=27.0676691729323, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17136276, Length=472, Percent_Identity=19.7033898305085, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56365; Mature: 56365

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDYKPRHTSADVVAAYGKCEIIEEGADPFVVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCEEHHH
RIIARRSFGKAAFVQLQDRKGRIQLYVRKDTLGDEAYEAFESFDIGDIIGATGTPFRTKT
HHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCC
GELSLNASEVRLLTKSFLPLPEKFHGLTDVETRYRQRYVDLIVNPEVREVFIKRSRIVNL
CCEECCHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IREFMVRHDFLEVETPMMQPIPGGATARPFVTHHNALDMELFLRIAPELYLKRLVVGGFE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVTQEVLGTLDFTY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
QGVEISFQRPWKRLTVKEAILEYGDIDAKSLEDRDLAYAYARSIGLDLPTDVGYGKLITE
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAEVKLIQPTFITAYPTEVSPLSRKSDKDPDIVDRFEFFCAGREMANAFSELNDPV
HHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCH
DQKERFLGQVAQKAKGDEEAHYMDEDYIRALEYGMPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEK
EEECCCCCCCC
>Mature Secondary Structure
MEELSELLLQRRRKVDALWEAGINPYPNDYKPRHTSADVVAAYGKCEIIEEGADPFVVAG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCEEHHH
RIIARRSFGKAAFVQLQDRKGRIQLYVRKDTLGDEAYEAFESFDIGDIIGATGTPFRTKT
HHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCC
GELSLNASEVRLLTKSFLPLPEKFHGLTDVETRYRQRYVDLIVNPEVREVFIKRSRIVNL
CCEECCHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IREFMVRHDFLEVETPMMQPIPGGATARPFVTHHNALDMELFLRIAPELYLKRLVVGGFE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RVFEINRNFRNEGISVRHNPEFTMMEFYQAYATFEDLMDFTEELLCHVTQEVLGTLDFTY
HHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
QGVEISFQRPWKRLTVKEAILEYGDIDAKSLEDRDLAYAYARSIGLDLPTDVGYGKLITE
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
IFEEVAEVKLIQPTFITAYPTEVSPLSRKSDKDPDIVDRFEFFCAGREMANAFSELNDPV
HHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCH
DQKERFLGQVAQKAKGDEEAHYMDEDYIRALEYGMPPTAGEGIGIDRLVMLLTDSPSIRD
HHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCCE
VILFPQLRKEK
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA