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The map label for this gene is rfaF [H]

Identifier: 78223548

GI number: 78223548

Start: 2652115

End: 2653194

Strand: Reverse

Name: rfaF [H]

Synonym: Gmet_2345

Alternate gene names: 78223548

Gene position: 2653194-2652115 (Counterclockwise)

Preceding gene: 78223549

Following gene: 78223547

Centisome position: 66.37

GC content: 63.52

Gene sequence:

>1080_bases
GTGACCGACAGCATATCGGCTCTCGATAAATCGGCTATACGCACCATTCTCGTCCGGGCGGTGAACTGGCTCGGCGATGC
GGTCATGACCACGCCTGCTCTTCGAGCAATTCGTGAGTCCTTCCCCGAAGCACGGATAACGGTTCTTGCAAATCCCCTTG
TGGCTGAACTTTTCGCCAATCACGAAACGGTCGATGCAGTTCATGTCTATGACCGGAAGGGAAGGCACGCCGGCATCCGG
GGAAGGATTAGACTGGCACGGGAGCTCAGGGCGGAGCGGTTCGACCTCGCCATCCTGTTGCAGAACGCTATCGACGCGGC
GCTCATTGCCCGGCTTGCCCGAATCCCCCGGATTATGGGGTACCGAACCGACGGACGGGGGATGCTCCTGACCCATGGCG
CACCGGTAACCATCGAAGCGAAAAAGCTCCACCATGTCGACTATTACCTCGCCATGCTTTCCCGATTCGGAATCGAAACC
GGGGCCAAACACCTCTCCCTGACCGTCACCCGGGAGGAGAAAGAGGGCACGGCCCGGCTTCTGGCCGCTGCGGGGATCGG
CGCGAACGATTTCGTCATCGGCATCAACCCCGGCGCCACCTATGGTTCGGCCAAGCGGTGGTACCCGGAGCGGTTTGCCG
CCGTGGCCGATGAGTTGTCTCTCCGCTGGGGGGCACGGGTCGTGGTGACTGGCGGACCGGGAGAGGCTGCCATCGCCGCC
GACATCGCCGCCGCCATGACCGTACCCGCCCTCGTCATGGCGGGGAAGACCTCGGTGCGGGAGTTGATGGCGCTCATCAA
ACGCTGCGACTTCTTCATCACCAACGACTCGGGCCCCATGCACATCGCCGCCGCCTTCAGTGTTCCGCTCGTGGCGGTGT
TTGGCCCCACTGATCACACGACCACCTCGCCCTGGAGCGACCGTGCCGCCGTCGTGCGCCGCGACACCGACTGCGCCCCG
TGCCTCCTGCGGGAGTGTCCCACGGACCACCGGTGCATGACAGCAGTGACAATGACCGACGTGGTGGAGGCCGCGGAGCG
GCTTCGGACGTGTGTTGCTGCTTTGGAGCATTCTGCATGA

Upstream 100 bases:

>100_bases
TCTGCGATTCATGTCGGCTCGTCTACCCCGTGCGGGACGGAATTCCGGTCATGCTGGTGGACGAAGCGGAAAAAATTGGA
GAAGCGGGGAAACGCGATTA

Downstream 100 bases:

>100_bases
ACATAACGGCCACCATCATCGCCAAGAACGAAGAGAAGAATATCTCTGACTGCCTTGCGAGCCTCGATTGGGCTGATGAG
ATTATCGTGGTTGATTCGGG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTDSISALDKSAIRTILVRAVNWLGDAVMTTPALRAIRESFPEARITVLANPLVAELFANHETVDAVHVYDRKGRHAGIR
GRIRLARELRAERFDLAILLQNAIDAALIARLARIPRIMGYRTDGRGMLLTHGAPVTIEAKKLHHVDYYLAMLSRFGIET
GAKHLSLTVTREEKEGTARLLAAAGIGANDFVIGINPGATYGSAKRWYPERFAAVADELSLRWGARVVVTGGPGEAAIAA
DIAAAMTVPALVMAGKTSVRELMALIKRCDFFITNDSGPMHIAAAFSVPLVAVFGPTDHTTTSPWSDRAAVVRRDTDCAP
CLLRECPTDHRCMTAVTMTDVVEAAERLRTCVAALEHSA

Sequences:

>Translated_359_residues
MTDSISALDKSAIRTILVRAVNWLGDAVMTTPALRAIRESFPEARITVLANPLVAELFANHETVDAVHVYDRKGRHAGIR
GRIRLARELRAERFDLAILLQNAIDAALIARLARIPRIMGYRTDGRGMLLTHGAPVTIEAKKLHHVDYYLAMLSRFGIET
GAKHLSLTVTREEKEGTARLLAAAGIGANDFVIGINPGATYGSAKRWYPERFAAVADELSLRWGARVVVTGGPGEAAIAA
DIAAAMTVPALVMAGKTSVRELMALIKRCDFFITNDSGPMHIAAAFSVPLVAVFGPTDHTTTSPWSDRAAVVRRDTDCAP
CLLRECPTDHRCMTAVTMTDVVEAAERLRTCVAALEHSA
>Mature_358_residues
TDSISALDKSAIRTILVRAVNWLGDAVMTTPALRAIRESFPEARITVLANPLVAELFANHETVDAVHVYDRKGRHAGIRG
RIRLARELRAERFDLAILLQNAIDAALIARLARIPRIMGYRTDGRGMLLTHGAPVTIEAKKLHHVDYYLAMLSRFGIETG
AKHLSLTVTREEKEGTARLLAAAGIGANDFVIGINPGATYGSAKRWYPERFAAVADELSLRWGARVVVTGGPGEAAIAAD
IAAAMTVPALVMAGKTSVRELMALIKRCDFFITNDSGPMHIAAAFSVPLVAVFGPTDHTTTSPWSDRAAVVRRDTDCAPC
LLRECPTDHRCMTAVTMTDVVEAAERLRTCVAALEHSA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=309, Percent_Identity=31.7152103559871, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790062, Length=334, Percent_Identity=29.0419161676647, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38871; Mature: 38740

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSISALDKSAIRTILVRAVNWLGDAVMTTPALRAIRESFPEARITVLANPLVAELFAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
HETVDAVHVYDRKGRHAGIRGRIRLARELRAERFDLAILLQNAIDAALIARLARIPRIMG
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YRTDGRGMLLTHGAPVTIEAKKLHHVDYYLAMLSRFGIETGAKHLSLTVTREEKEGTARL
CCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHH
LAAAGIGANDFVIGINPGATYGSAKRWYPERFAAVADELSLRWGARVVVTGGPGEAAIAA
HHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
DIAAAMTVPALVMAGKTSVRELMALIKRCDFFITNDSGPMHIAAAFSVPLVAVFGPTDHT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCEEEEECCCCCC
TTSPWSDRAAVVRRDTDCAPCLLRECPTDHRCMTAVTMTDVVEAAERLRTCVAALEHSA
CCCCCCCCHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDSISALDKSAIRTILVRAVNWLGDAVMTTPALRAIRESFPEARITVLANPLVAELFAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
HETVDAVHVYDRKGRHAGIRGRIRLARELRAERFDLAILLQNAIDAALIARLARIPRIMG
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YRTDGRGMLLTHGAPVTIEAKKLHHVDYYLAMLSRFGIETGAKHLSLTVTREEKEGTARL
CCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHH
LAAAGIGANDFVIGINPGATYGSAKRWYPERFAAVADELSLRWGARVVVTGGPGEAAIAA
HHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHH
DIAAAMTVPALVMAGKTSVRELMALIKRCDFFITNDSGPMHIAAAFSVPLVAVFGPTDHT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCEEEEECCCCCC
TTSPWSDRAAVVRRDTDCAPCLLRECPTDHRCMTAVTMTDVVEAAERLRTCVAALEHSA
CCCCCCCCHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]