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The map label for this gene is waaE [H]

Identifier: 78223547

GI number: 78223547

Start: 2651369

End: 2652118

Strand: Reverse

Name: waaE [H]

Synonym: Gmet_2344

Alternate gene names: 78223547

Gene position: 2652118-2651369 (Counterclockwise)

Preceding gene: 78223548

Following gene: 78223546

Centisome position: 66.35

GC content: 55.47

Gene sequence:

>750_bases
ATGAACATAACGGCCACCATCATCGCCAAGAACGAAGAGAAGAATATCTCTGACTGCCTTGCGAGCCTCGATTGGGCTGA
TGAGATTATCGTGGTTGATTCGGGGAGCTCCGACAGGACTCCCGAAATCTGTCGCAAGCACCCCAAGGTGCGATATTTCG
AGCATGAGTGGGAAGGCTTCGGCAAACAGAAGAACTTCGCCGCCGATCAGGCTGTAAATGATTGGGTGTTCAATATCGAT
GCGGATGAGCGTGTTTCACCGGAACTGCGGGACTCGATCCTTTCCACTGATTTTGCCCGTTACGACGGTTTCCGCGTGGC
GCGGGAGAATTATTTCGGCCGACGCTGGATCAAACACTGCGGCTGGTACCCTGACCATAATCTGCGACTGTACAACCGGA
GCCGGTGCCGCTTCGCCGAGCGCCTCGTCCACGAATCCGTTGAGTGCCCCGGTCCGGTCAGTACCCTGCAAGGGAATCTG
ATCCACTTCACCTACGAGGGGATCGGTGATTACGTCGCCAGGATGGACCGATACTCGACGCTGGCGGCGGAAGAGATTGC
CAGGTCAGGAAGAAGGCCGGGGGTCTTCTCCATCGTTTTCCGGCCCTGTTTCACCTTTTTCAAGATGTATGTGCTGCGGA
TGGGAGTTCTGGAAGGCAGCACCGGCCTCCTCCTTTCACTGCTGTACGCCGTCTACACATTTCTCAAGTATGCAAAGTCC
GTTGAATTGATCGAGGAGCGCCAGCGATAG

Upstream 100 bases:

>100_bases
GTCCCACGGACCACCGGTGCATGACAGCAGTGACAATGACCGACGTGGTGGAGGCCGCGGAGCGGCTTCGGACGTGTGTT
GCTGCTTTGGAGCATTCTGC

Downstream 100 bases:

>100_bases
TCAACGTGTGCGCACCTCTGGTCGCGGGAATATATCCGTTATGCCGAAACTGTATATAAATGAATTCGTCGGTATCTCTA
ATCGACTGGAAACACTGCCC

Product: beta-1,4-glucosyltransferase

Products: UDP; (1,4-beta-D-glucosyl)n+1

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNITATIIAKNEEKNISDCLASLDWADEIIVVDSGSSDRTPEICRKHPKVRYFEHEWEGFGKQKNFAADQAVNDWVFNID
ADERVSPELRDSILSTDFARYDGFRVARENYFGRRWIKHCGWYPDHNLRLYNRSRCRFAERLVHESVECPGPVSTLQGNL
IHFTYEGIGDYVARMDRYSTLAAEEIARSGRRPGVFSIVFRPCFTFFKMYVLRMGVLEGSTGLLLSLLYAVYTFLKYAKS
VELIEERQR

Sequences:

>Translated_249_residues
MNITATIIAKNEEKNISDCLASLDWADEIIVVDSGSSDRTPEICRKHPKVRYFEHEWEGFGKQKNFAADQAVNDWVFNID
ADERVSPELRDSILSTDFARYDGFRVARENYFGRRWIKHCGWYPDHNLRLYNRSRCRFAERLVHESVECPGPVSTLQGNL
IHFTYEGIGDYVARMDRYSTLAAEEIARSGRRPGVFSIVFRPCFTFFKMYVLRMGVLEGSTGLLLSLLYAVYTFLKYAKS
VELIEERQR
>Mature_249_residues
MNITATIIAKNEEKNISDCLASLDWADEIIVVDSGSSDRTPEICRKHPKVRYFEHEWEGFGKQKNFAADQAVNDWVFNID
ADERVSPELRDSILSTDFARYDGFRVARENYFGRRWIKHCGWYPDHNLRLYNRSRCRFAERLVHESVECPGPVSTLQGNL
IHFTYEGIGDYVARMDRYSTLAAEEIARSGRRPGVFSIVFRPCFTFFKMYVLRMGVLEGSTGLLLSLLYAVYTFLKYAKS
VELIEERQR

Specific function: Unknown

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family. WaaE/kdtX subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.12

Molecular weight: Translated: 28939; Mature: 28939

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITATIIAKNEEKNISDCLASLDWADEIIVVDSGSSDRTPEICRKHPKVRYFEHEWEGF
CCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCEEECHHHHCC
GKQKNFAADQAVNDWVFNIDADERVSPELRDSILSTDFARYDGFRVARENYFGRRWIKHC
CCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GWYPDHNLRLYNRSRCRFAERLVHESVECPGPVSTLQGNLIHFTYEGIGDYVARMDRYST
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHH
LAAEEIARSGRRPGVFSIVFRPCFTFFKMYVLRMGVLEGSTGLLLSLLYAVYTFLKYAKS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VELIEERQR
HHHHHHHCC
>Mature Secondary Structure
MNITATIIAKNEEKNISDCLASLDWADEIIVVDSGSSDRTPEICRKHPKVRYFEHEWEGF
CCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCEEECHHHHCC
GKQKNFAADQAVNDWVFNIDADERVSPELRDSILSTDFARYDGFRVARENYFGRRWIKHC
CCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GWYPDHNLRLYNRSRCRFAERLVHESVECPGPVSTLQGNLIHFTYEGIGDYVARMDRYST
CCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHH
LAAEEIARSGRRPGVFSIVFRPCFTFFKMYVLRMGVLEGSTGLLLSLLYAVYTFLKYAKS
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VELIEERQR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; (1,4-beta-D-glucosyl)n

Specific reaction: UDP-glucose + (1,4-beta-D-glucosyl)n = UDP + (1,4-beta-D-glucosyl)n+1

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11371519 [H]