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The map label for this gene is yagI [H]

Identifier: 78223463

GI number: 78223463

Start: 2559034

End: 2559816

Strand: Reverse

Name: yagI [H]

Synonym: Gmet_2259

Alternate gene names: 78223463

Gene position: 2559816-2559034 (Counterclockwise)

Preceding gene: 78223464

Following gene: 78223462

Centisome position: 64.04

GC content: 47.13

Gene sequence:

>783_bases
ATGAAGACAAGAGATTATACAATTCAATCGGTTAGGCATGCACTCGATATCCTGGAACAATTTCGCGGGAATTCGTCCGA
GCTGTGTGTGATGGATTTGAGTCGGAGGTTACAGCTCCCAAAAAACAAAATTTACAAACTGATAGCAACACTCGAACATC
ACAATTATCTGGAACAAAACATAAGCACAAAGAACTATCGCCTCGGTATAAAAAATATGGAACTTGGGCAGGTAGCGATG
CGACGAATGCGGCGACTTGGAGATTCCAGGTCGGTCATGGAGGCACTTGTCAGGGAATGCAACGAAACCCTGTGTGTATC
AATACTCAAAGGTTCTCATGCGGTTAACATGGATGCAGTCGTGGGAGACAACCACTTGCGGGCAATTCCCCCTATTGGCA
TAAGTATTCCTGCACACTGCACGGCTGCCGGGAAGGTACTAATCGCCGGTCTAACAGAGGAGCAGCGGTTGCACTATGTT
TCTAGCTGTTTGTTCAAGCGTTACACAACGGCAACTATAACCAATCCGAAACTGTTTCTGCGACATCTGGAAGAAGTTGA
CCGGCAAGGGTATGCCCTCGAGTTGGAGGAACAGGAAGTTGGAGTTAAATCGGTTTCGGCCCCGATACGGGACTACACGT
CAAGGACAATTGGCGCGATTACCGTGTACGGACCATCCACGCGATTTCCTGATGACCGAATGGAAAAAGAATTGATTCCG
CTTGTTCTCAAGGGAGCAATGGAAATTTCGGACATGTTGGGATATCGACAAAGTGAATTCTGA

Upstream 100 bases:

>100_bases
ATGTACAAGCGTAGTATAGGTTAATTAACTGTGCACCTGTAGTATAGCTTTAAAAATGTTTCTGGCATATAGTTAACAGA
GATGTTGTAGGCAATGTTAT

Downstream 100 bases:

>100_bases
ATTTGAGAAATAGCTGGTTGCACTTTATGGTTTTCCGTTGTTGCATATAGACTCCTGTCGAGCCGTAAGCACCCCAAACA
ATTTTTATCTGCCCGGTCCG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKTRDYTIQSVRHALDILEQFRGNSSELCVMDLSRRLQLPKNKIYKLIATLEHHNYLEQNISTKNYRLGIKNMELGQVAM
RRMRRLGDSRSVMEALVRECNETLCVSILKGSHAVNMDAVVGDNHLRAIPPIGISIPAHCTAAGKVLIAGLTEEQRLHYV
SSCLFKRYTTATITNPKLFLRHLEEVDRQGYALELEEQEVGVKSVSAPIRDYTSRTIGAITVYGPSTRFPDDRMEKELIP
LVLKGAMEISDMLGYRQSEF

Sequences:

>Translated_260_residues
MKTRDYTIQSVRHALDILEQFRGNSSELCVMDLSRRLQLPKNKIYKLIATLEHHNYLEQNISTKNYRLGIKNMELGQVAM
RRMRRLGDSRSVMEALVRECNETLCVSILKGSHAVNMDAVVGDNHLRAIPPIGISIPAHCTAAGKVLIAGLTEEQRLHYV
SSCLFKRYTTATITNPKLFLRHLEEVDRQGYALELEEQEVGVKSVSAPIRDYTSRTIGAITVYGPSTRFPDDRMEKELIP
LVLKGAMEISDMLGYRQSEF
>Mature_260_residues
MKTRDYTIQSVRHALDILEQFRGNSSELCVMDLSRRLQLPKNKIYKLIATLEHHNYLEQNISTKNYRLGIKNMELGQVAM
RRMRRLGDSRSVMEALVRECNETLCVSILKGSHAVNMDAVVGDNHLRAIPPIGISIPAHCTAAGKVLIAGLTEEQRLHYV
SSCLFKRYTTATITNPKLFLRHLEEVDRQGYALELEEQEVGVKSVSAPIRDYTSRTIGAITVYGPSTRFPDDRMEKELIP
LVLKGAMEISDMLGYRQSEF

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=32.258064516129, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788131, Length=254, Percent_Identity=27.9527559055118, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082359, Length=249, Percent_Identity=26.1044176706827, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786715, Length=248, Percent_Identity=25.8064516129032, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790752, Length=230, Percent_Identity=28.695652173913, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789999, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29511; Mature: 29511

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRDYTIQSVRHALDILEQFRGNSSELCVMDLSRRLQLPKNKIYKLIATLEHHNYLEQN
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHC
ISTKNYRLGIKNMELGQVAMRRMRRLGDSRSVMEALVRECNETLCVSILKGSHAVNMDAV
CCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEE
VGDNHLRAIPPIGISIPAHCTAAGKVLIAGLTEEQRLHYVSSCLFKRYTTATITNPKLFL
ECCCCCEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEECCHHHHH
RHLEEVDRQGYALELEEQEVGVKSVSAPIRDYTSRTIGAITVYGPSTRFPDDRMEKELIP
HHHHHHHHCCCEEEEHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
LVLKGAMEISDMLGYRQSEF
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKTRDYTIQSVRHALDILEQFRGNSSELCVMDLSRRLQLPKNKIYKLIATLEHHNYLEQN
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHC
ISTKNYRLGIKNMELGQVAMRRMRRLGDSRSVMEALVRECNETLCVSILKGSHAVNMDAV
CCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEE
VGDNHLRAIPPIGISIPAHCTAAGKVLIAGLTEEQRLHYVSSCLFKRYTTATITNPKLFL
ECCCCCEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEECCHHHHH
RHLEEVDRQGYALELEEQEVGVKSVSAPIRDYTSRTIGAITVYGPSTRFPDDRMEKELIP
HHHHHHHHCCCEEEEHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHH
LVLKGAMEISDMLGYRQSEF
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]