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The map label for this gene is fixA [H]

Identifier: 78223462

GI number: 78223462

Start: 2557624

End: 2558439

Strand: Reverse

Name: fixA [H]

Synonym: Gmet_2258

Alternate gene names: 78223462

Gene position: 2558439-2557624 (Counterclockwise)

Preceding gene: 78223463

Following gene: 78223461

Centisome position: 64.0

GC content: 64.34

Gene sequence:

>816_bases
ATGCTCATCGTAGTCTGCATCAAACAGGTACCGGATACGACCCAGGTCCAGATCGACCCGGTGACCAACACCCTGGTGCG
GGAGGGGATACCGTTCATTGTCAACCCCTACGACACCCATGCCCTGGAGGAGGCCCTGCGGCTCAAAGACCGGTTCGGGT
ACAGGGTCGCTGCGCTTTCCATGGGGCCGCCCAACGCCGAGGCCACCTTGAAGAAAGCACTGGCCCTGGGGGTCGACCGG
GCCATCCTCCTGTCCGACCGGGTCTTCGGCGGTGCCGATACCCTGGCCACCAGCAACGTCCTTTCCGCCGCCATCCGCAA
GCTGCACGATGAGGTGGACAAGGTCAGCCTCGTACTCTGTGGCAAGCAGACCATCGACGGCGATACCGCCCAGGTGGGGC
CGGGTATCGCCAGCCGCCTCAACTTCCGGCAGCTCACCCTCGTGGACCGGATCGAGAACCTGGATGCAGCGGGGGGGAAG
ATCCGTGTCAGCCGCAAACTGGAAGGGCGCCACGAAATCGTGGAAACCACCCTGCCGGCCATGCTCACCGTCGTGCGTGA
ACTGAACCGACCCCGCTACCCAAAGGTCTCCATGCGGCTGGCGGCGGCGGAAGCGACGGTGGAGGTCTGGAACAACCAGA
CCCTTGGGCTCGACGAACAGAGCATCGGCCTCAAGGGTTCCCCCACCTGGGTCAGCAAGATCTTCTCCCCCGAGCGGGCC
AAGGGTGAGATCCTCGGCGACGGCATTGCCGAGCCGGCGGCGGCGACCCGGATGCTGATTGACAGGTTGCTTTCCAAAGA
CCTGCTGGCGCTGTAA

Upstream 100 bases:

>100_bases
CATGACTGCGGAATGCCATGGGTAGTGCTGGATACGAAAAAGCGATTGCCAGGATAGTTGGCACAAGCGTAAGGCAATAC
AGGATAAGCGAGGATAACCC

Downstream 100 bases:

>100_bases
CGGCTGCCGCTTTCGCGCAATCTCGGCGTTGCCCTGCGGTCTGCTCTTGTGCGGCGTAGCGCTGCTACGCCTCCGCGGCA
TTCCTTGGGCGCCTTGACCT

Product: electron transfer flavoprotein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLIVVCIKQVPDTTQVQIDPVTNTLVREGIPFIVNPYDTHALEEALRLKDRFGYRVAALSMGPPNAEATLKKALALGVDR
AILLSDRVFGGADTLATSNVLSAAIRKLHDEVDKVSLVLCGKQTIDGDTAQVGPGIASRLNFRQLTLVDRIENLDAAGGK
IRVSRKLEGRHEIVETTLPAMLTVVRELNRPRYPKVSMRLAAAEATVEVWNNQTLGLDEQSIGLKGSPTWVSKIFSPERA
KGEILGDGIAEPAAATRMLIDRLLSKDLLAL

Sequences:

>Translated_271_residues
MLIVVCIKQVPDTTQVQIDPVTNTLVREGIPFIVNPYDTHALEEALRLKDRFGYRVAALSMGPPNAEATLKKALALGVDR
AILLSDRVFGGADTLATSNVLSAAIRKLHDEVDKVSLVLCGKQTIDGDTAQVGPGIASRLNFRQLTLVDRIENLDAAGGK
IRVSRKLEGRHEIVETTLPAMLTVVRELNRPRYPKVSMRLAAAEATVEVWNNQTLGLDEQSIGLKGSPTWVSKIFSPERA
KGEILGDGIAEPAAATRMLIDRLLSKDLLAL
>Mature_271_residues
MLIVVCIKQVPDTTQVQIDPVTNTLVREGIPFIVNPYDTHALEEALRLKDRFGYRVAALSMGPPNAEATLKKALALGVDR
AILLSDRVFGGADTLATSNVLSAAIRKLHDEVDKVSLVLCGKQTIDGDTAQVGPGIASRLNFRQLTLVDRIENLDAAGGK
IRVSRKLEGRHEIVETTLPAMLTVVRELNRPRYPKVSMRLAAAEATVEVWNNQTLGLDEQSIGLKGSPTWVSKIFSPERA
KGEILGDGIAEPAAATRMLIDRLLSKDLLAL

Specific function: May play a role in a redox process involved in nitrogen fixation [H]

COG id: COG2086

COG function: function code C; Electron transfer flavoprotein, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF beta-subunit/fixA family [H]

Homologues:

Organism=Homo sapiens, GI4503609, Length=218, Percent_Identity=33.4862385321101, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI62420877, Length=205, Percent_Identity=34.1463414634146, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI87081682, Length=263, Percent_Identity=29.277566539924, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI2367123, Length=213, Percent_Identity=27.2300469483568, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25141345, Length=197, Percent_Identity=31.9796954314721, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321646, Length=213, Percent_Identity=33.8028169014084, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24651147, Length=194, Percent_Identity=32.9896907216495, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651145, Length=194, Percent_Identity=32.9896907216495, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000049
- InterPro:   IPR014730
- InterPro:   IPR012255
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01012 ETF [H]

EC number: NA

Molecular weight: Translated: 29435; Mature: 29435

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVVCIKQVPDTTQVQIDPVTNTLVREGIPFIVNPYDTHALEEALRLKDRFGYRVAALS
CEEEEEECCCCCCCEEEECCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEEE
MGPPNAEATLKKALALGVDRAILLSDRVFGGADTLATSNVLSAAIRKLHDEVDKVSLVLC
CCCCCHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
GKQTIDGDTAQVGPGIASRLNFRQLTLVDRIENLDAAGGKIRVSRKLEGRHEIVETTLPA
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
MLTVVRELNRPRYPKVSMRLAAAEATVEVWNNQTLGLDEQSIGLKGSPTWVSKIFSPERA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHCCCCCC
KGEILGDGIAEPAAATRMLIDRLLSKDLLAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIVVCIKQVPDTTQVQIDPVTNTLVREGIPFIVNPYDTHALEEALRLKDRFGYRVAALS
CEEEEEECCCCCCCEEEECCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEEE
MGPPNAEATLKKALALGVDRAILLSDRVFGGADTLATSNVLSAAIRKLHDEVDKVSLVLC
CCCCCHHHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
GKQTIDGDTAQVGPGIASRLNFRQLTLVDRIENLDAAGGKIRVSRKLEGRHEIVETTLPA
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHH
MLTVVRELNRPRYPKVSMRLAAAEATVEVWNNQTLGLDEQSIGLKGSPTWVSKIFSPERA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHCCCCCC
KGEILGDGIAEPAAATRMLIDRLLSKDLLAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA