BacMap

Definition	Geobacter metallireducens GS-15 chromosome, complete genome.
Accession	NC_007517
Length	3,997,420

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The map label for this gene is paaF [C]

Identifier: 78223417

GI number: 78223417

Start: 2503603

End: 2504391

Strand: Reverse

Name: paaF [C]

Synonym: Gmet_2212

Alternate gene names: 78223417

Gene position: 2504391-2503603 (Counterclockwise)

Preceding gene: 78223418

Following gene: 78223416

Centisome position: 62.65

GC content: 55.39

Gene sequence:

>789_bases
ATGAGTTATGAAGCAATCATGCTGGAGCGTAACGGAGCAGTTGGAGTTCTGACTTTTAATCGGCCAGAGGTATTGAATGC
ATACAACCGGACCCTTGCCGCAGATATTATAACGGGATTCAACGAGCTTGTAGCGGACAAATCGGTGAGGGCAATCGTAT
TGACCGGTGCCGGCAAGGCGTTCATGGCCGGTGCGGACATCAATATGGTCAATGGCTGGACCAAGTTGGGGAATGCCGCA
AAGATCAAGGAGGATCTCAGGCAACTGGTCAATCCGAATATGATAGAGGATTGTCCCAAGCCGACCATTGCTGCAGTAAA
CGGTCTTGCCTTTGGCATGGGATGCGAGCTTGCCATGGCCTGTGATTTTCGGATTGCAGCAGAAAAAGCCCAATTCGGCC
AGCCCGAGGTAAAGCTCGGCATCATTCCCGGTGCAGGGGGGAGCCAGCGTCTGCGGGAACTTGTCGGGCCGACCAGGGCG
CTGGAGATGATTTCCACCGGCGACCCCATTGATGCCCAGGAGGCCTACAGGATTGGCCTTGTCAACCAGGTGGTCCCCCG
CGATGAGTTGATGGAGGCGGTCAACGCGTTTGCCGGCAGGCTGATCGACAAGGGAGCGGTAGTGCTGGATATCTGCAAGA
AGCTGGTCTACGAAGGGGGTGATCTACCTCTCCGTGGGGGGATCGATTACGAGCAGGACCAGTTCTGCAAAATCCTCCTG
ACAGCAGATGCACAGGAAGGGACTCTGGCCTTCCTGGAAAAGCGCAAGCCGGTCTTCAACAAGGGTTAG

Upstream 100 bases:

>100_bases
AACCTTCTCGAAAAAATTTAAGAACAGGAATTTTTGTACTTGACATGTAAACCGAACGGTCGTACTATTCGGATAACTGA
TAGGAAGAGAGGGTCGGATA

Downstream 100 bases:

>100_bases
TAGACACAGGTGTTCAATCCTCCACAAAAGAAAGACGGATGCCATGGCAAATCTGTTAGTCGATGAACGTGATGTGAAAT
TCGCCCTTTTTGAGCAGTTA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSYEAIMLERNGAVGVLTFNRPEVLNAYNRTLAADIITGFNELVADKSVRAIVLTGAGKAFMAGADINMVNGWTKLGNAA
KIKEDLRQLVNPNMIEDCPKPTIAAVNGLAFGMGCELAMACDFRIAAEKAQFGQPEVKLGIIPGAGGSQRLRELVGPTRA
LEMISTGDPIDAQEAYRIGLVNQVVPRDELMEAVNAFAGRLIDKGAVVLDICKKLVYEGGDLPLRGGIDYEQDQFCKILL
TADAQEGTLAFLEKRKPVFNKG

Sequences:

>Translated_262_residues
MSYEAIMLERNGAVGVLTFNRPEVLNAYNRTLAADIITGFNELVADKSVRAIVLTGAGKAFMAGADINMVNGWTKLGNAA
KIKEDLRQLVNPNMIEDCPKPTIAAVNGLAFGMGCELAMACDFRIAAEKAQFGQPEVKLGIIPGAGGSQRLRELVGPTRA
LEMISTGDPIDAQEAYRIGLVNQVVPRDELMEAVNAFAGRLIDKGAVVLDICKKLVYEGGDLPLRGGIDYEQDQFCKILL
TADAQEGTLAFLEKRKPVFNKG
>Mature_261_residues
SYEAIMLERNGAVGVLTFNRPEVLNAYNRTLAADIITGFNELVADKSVRAIVLTGAGKAFMAGADINMVNGWTKLGNAAK
IKEDLRQLVNPNMIEDCPKPTIAAVNGLAFGMGCELAMACDFRIAAEKAQFGQPEVKLGIIPGAGGSQRLRELVGPTRAL
EMISTGDPIDAQEAYRIGLVNQVVPRDELMEAVNAFAGRLIDKGAVVLDICKKLVYEGGDLPLRGGIDYEQDQFCKILLT
ADAQEGTLAFLEKRKPVFNKG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=263, Percent_Identity=41.06463878327, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI68989263, Length=280, Percent_Identity=32.5, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=37.9888268156425, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=33.4630350194552, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.9548872180451, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31542718, Length=235, Percent_Identity=31.4893617021277, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=30.9278350515464, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=30.9278350515464, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=28.5087719298246, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI261878539, Length=182, Percent_Identity=32.4175824175824, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=27.4131274131274, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=27.4131274131274, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI25453481, Length=258, Percent_Identity=27.5193798449612, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI51538573, Length=258, Percent_Identity=27.5193798449612, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260275230, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260274832, Length=203, Percent_Identity=30.5418719211823, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4757966, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538581, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI157694520, Length=187, Percent_Identity=28.3422459893048, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221136756, Length=253, Percent_Identity=26.8774703557312, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221136753, Length=253, Percent_Identity=26.8774703557312, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI221307494, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=25.8064516129032, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI116235487, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI62530384, Length=192, Percent_Identity=28.125, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=36.5384615384615, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=35.2490421455939, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=37.17277486911, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=33.5907335907336, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=30.5343511450382, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=34.020618556701, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=30.0380228136882, Blast_Score=104, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=39.2307692307692, Blast_Score=172, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=38.0769230769231, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=30.8695652173913, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=38.7978142076503, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=36.6120218579235, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=183, Percent_Identity=36.6120218579235, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508953, Length=183, Percent_Identity=36.6120218579235, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=258, Percent_Identity=29.0697674418605, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=276, Percent_Identity=25.7246376811594, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=255, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=29.4930875576037, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=28.957528957529, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=28.7958115183246, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=228, Percent_Identity=29.3859649122807, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=201, Percent_Identity=26.865671641791, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=268, Percent_Identity=41.4179104477612, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24653477, Length=268, Percent_Identity=41.4179104477612, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=35.6060606060606, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=26.953125, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=31.3725490196078, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654903, Length=198, Percent_Identity=29.7979797979798, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357171, Length=181, Percent_Identity=32.5966850828729, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24650670, Length=197, Percent_Identity=31.9796954314721, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921000, Length=167, Percent_Identity=34.1317365269461, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583077, Length=167, Percent_Identity=34.1317365269461, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583079, Length=167, Percent_Identity=34.1317365269461, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=29.5918367346939, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571729, Length=157, Percent_Identity=28.6624203821656, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571730, Length=157, Percent_Identity=28.6624203821656, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583165, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28204; Mature: 28073

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEAIMLERNGAVGVLTFNRPEVLNAYNRTLAADIITGFNELVADKSVRAIVLTGAGKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
FMAGADINMVNGWTKLGNAAKIKEDLRQLVNPNMIEDCPKPTIAAVNGLAFGMGCELAMA
EEECCCCEEECCHHHHCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHEECCCCCEEEE
CDFRIAAEKAQFGQPEVKLGIIPGAGGSQRLRELVGPTRALEMISTGDPIDAQEAYRIGL
ECEEEEHHHHHCCCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHH
VNQVVPRDELMEAVNAFAGRLIDKGAVVLDICKKLVYEGGDLPLRGGIDYEQDQFCKILL
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
TADAQEGTLAFLEKRKPVFNKG
ECCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYEAIMLERNGAVGVLTFNRPEVLNAYNRTLAADIITGFNELVADKSVRAIVLTGAGKA
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCE
FMAGADINMVNGWTKLGNAAKIKEDLRQLVNPNMIEDCPKPTIAAVNGLAFGMGCELAMA
EEECCCCEEECCHHHHCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHEECCCCCEEEE
CDFRIAAEKAQFGQPEVKLGIIPGAGGSQRLRELVGPTRALEMISTGDPIDAQEAYRIGL
ECEEEEHHHHHCCCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHH
VNQVVPRDELMEAVNAFAGRLIDKGAVVLDICKKLVYEGGDLPLRGGIDYEQDQFCKILL
HHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
TADAQEGTLAFLEKRKPVFNKG
ECCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA