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The map label for this gene is thlA [H]

Identifier: 78223418

GI number: 78223418

Start: 2504492

End: 2505670

Strand: Reverse

Name: thlA [H]

Synonym: Gmet_2213

Alternate gene names: 78223418

Gene position: 2505670-2504492 (Counterclockwise)

Preceding gene: 78223419

Following gene: 78223417

Centisome position: 62.68

GC content: 58.95

Gene sequence:

>1179_bases
ATGGAAGAAGCAGTCATAGTCGGAGCAGCCCGTACGGCGATTGGAGATTTCATGGGGGCACTCAAGGATGTGTCCGCGGT
GGATCTGGGCATTGCTGCCGTTCAGGGGGCCTTGAAAAAGGCCGGGATTGGTCCGGAACTGGTGGAAGAGGTTGTCGGGG
GGATGATCTACAAGGCAGGAGCCAAGGGGAACCCGGCGCGCCAGGTCCAGCTCAAGACGGGAATACCTCTCGAGGGGGCA
GCCACTACGGTGGAGCAGTTGTGCGGGTCAGCCATGCGGGCACTGGAAATCGCCTCGCAGCAGATACTCCTTGGGAAGAC
GTCTGTAGGTGTTGCTGTAGGGATGGAGAGCATGTCCCAGGCTCCCTATCTGCTCCTCAATGCCCGTCAGGGCCTGCGCC
TCGGTTCAGATTCTATCGAGGATCATCTTCTGCACGATGCGTTGATCGATGCTTTCAGCGGTACGCACATGGGGATAACC
GCTGAGAACCTGGCGGAGAAATACGGCATTTCCCGCGAGGAACAGGATGATCTGGCTTTGTTGAGCCATCAGCGGGCCGT
TGCGGCCGCGGAGTCAGGGAAATTCAGGGCTGAAATCGTGCCGGTGGAAGTGAAGAGTCGCAAGGGGACCATCGTCATCG
ACACCGATGAGCATCCCCGGGCGGACATGAGCCGGGAGAAGCTGGCCGCCCTGAGACCGGCATTCAAAAAAGATGGCACG
GTCACGGCGGGCAACGCTTCGAGCATCAACGATGGTGCTGCCGCGCTTGTGCTCATGACAGCAAGGCGGGCCAGGGAGCT
TGGGGTGAGCCCCATTGCCCGGATACGGGCTACGGCTTCCTTCGGTGTTGCACCGGAGATCATGGGAATCGGGCCGGCCT
ATGCCATTCCCAAGGCTTTGAAATATGCCGACCTGCAGATGAAAGACATCGACTACTTTGAAATCAACGAAGCCTTTGCG
GCCCAATTTCTGGCCGTGAATCGTGAGCTGAACATCGACCTCTCCCGTGTGAATGCGAACGGGTCGGGGATTGCCCTGGG
GCACCCCGTTGGGTGCACGGGGATACGGATCATCGTGACCATGCTGGAAGAGATGAAACGGCGGCAAGGTAGATACGGGG
TTGCTTCCCTCTGTGTCGGTGGTGGACCGGCAATGGCAACGGTCATAGAGATGATCTGA

Upstream 100 bases:

>100_bases
GGAGATTCGGGGCTCTCTCGGCACTGTCAGAAAAAAGGCGTGATGAAATAGTTCTTGATTTTATGCAGCAGTGAAACTAC
GCAAACTGGAGGCCGATTTC

Downstream 100 bases:

>100_bases
AACCTTCTCGAAAAAATTTAAGAACAGGAATTTTTGTACTTGACATGTAAACCGAACGGTCGTACTATTCGGATAACTGA
TAGGAAGAGAGGGTCGGATA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MEEAVIVGAARTAIGDFMGALKDVSAVDLGIAAVQGALKKAGIGPELVEEVVGGMIYKAGAKGNPARQVQLKTGIPLEGA
ATTVEQLCGSAMRALEIASQQILLGKTSVGVAVGMESMSQAPYLLLNARQGLRLGSDSIEDHLLHDALIDAFSGTHMGIT
AENLAEKYGISREEQDDLALLSHQRAVAAAESGKFRAEIVPVEVKSRKGTIVIDTDEHPRADMSREKLAALRPAFKKDGT
VTAGNASSINDGAAALVLMTARRARELGVSPIARIRATASFGVAPEIMGIGPAYAIPKALKYADLQMKDIDYFEINEAFA
AQFLAVNRELNIDLSRVNANGSGIALGHPVGCTGIRIIVTMLEEMKRRQGRYGVASLCVGGGPAMATVIEMI

Sequences:

>Translated_392_residues
MEEAVIVGAARTAIGDFMGALKDVSAVDLGIAAVQGALKKAGIGPELVEEVVGGMIYKAGAKGNPARQVQLKTGIPLEGA
ATTVEQLCGSAMRALEIASQQILLGKTSVGVAVGMESMSQAPYLLLNARQGLRLGSDSIEDHLLHDALIDAFSGTHMGIT
AENLAEKYGISREEQDDLALLSHQRAVAAAESGKFRAEIVPVEVKSRKGTIVIDTDEHPRADMSREKLAALRPAFKKDGT
VTAGNASSINDGAAALVLMTARRARELGVSPIARIRATASFGVAPEIMGIGPAYAIPKALKYADLQMKDIDYFEINEAFA
AQFLAVNRELNIDLSRVNANGSGIALGHPVGCTGIRIIVTMLEEMKRRQGRYGVASLCVGGGPAMATVIEMI
>Mature_392_residues
MEEAVIVGAARTAIGDFMGALKDVSAVDLGIAAVQGALKKAGIGPELVEEVVGGMIYKAGAKGNPARQVQLKTGIPLEGA
ATTVEQLCGSAMRALEIASQQILLGKTSVGVAVGMESMSQAPYLLLNARQGLRLGSDSIEDHLLHDALIDAFSGTHMGIT
AENLAEKYGISREEQDDLALLSHQRAVAAAESGKFRAEIVPVEVKSRKGTIVIDTDEHPRADMSREKLAALRPAFKKDGT
VTAGNASSINDGAAALVLMTARRARELGVSPIARIRATASFGVAPEIMGIGPAYAIPKALKYADLQMKDIDYFEINEAFA
AQFLAVNRELNIDLSRVNANGSGIALGHPVGCTGIRIIVTMLEEMKRRQGRYGVASLCVGGGPAMATVIEMI

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=46.8030690537084, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI167614485, Length=387, Percent_Identity=45.2196382428941, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI4557237, Length=395, Percent_Identity=44.0506329113924, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=42.0253164556962, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=31.7647058823529, Blast_Score=181, Evalue=7e-46,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=29.6675191815857, Blast_Score=138, Evalue=9e-33,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=49.7435897435897, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1788554, Length=391, Percent_Identity=52.9411764705882, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=40.2985074626866, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=39.6039603960396, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1788683, Length=407, Percent_Identity=34.3980343980344, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=44.3298969072165, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI25147385, Length=391, Percent_Identity=37.5959079283887, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=38.676844783715, Blast_Score=263, Evalue=9e-71,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=32.8638497652582, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=395, Percent_Identity=30.126582278481, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=41.25, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6322031, Length=397, Percent_Identity=40.3022670025189, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=45.1530612244898, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=40.8163265306122, Blast_Score=284, Evalue=6e-77,
Organism=Drosophila melanogaster, GI17648125, Length=389, Percent_Identity=40.8740359897172, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI17137578, Length=388, Percent_Identity=35.8247422680412, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41174; Mature: 41174

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEAVIVGAARTAIGDFMGALKDVSAVDLGIAAVQGALKKAGIGPELVEEVVGGMIYKAG
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECC
AKGNPARQVQLKTGIPLEGAATTVEQLCGSAMRALEIASQQILLGKTSVGVAVGMESMSQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCHHHHCC
APYLLLNARQGLRLGSDSIEDHLLHDALIDAFSGTHMGITAENLAEKYGISREEQDDLAL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LSHQRAVAAAESGKFRAEIVPVEVKSRKGTIVIDTDEHPRADMSREKLAALRPAFKKDGT
HHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
VTAGNASSINDGAAALVLMTARRARELGVSPIARIRATASFGVAPEIMGIGPAYAIPKAL
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHH
KYADLQMKDIDYFEINEAFAAQFLAVNRELNIDLSRVNANGSGIALGHPVGCTGIRIIVT
HHCCCEECCCCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHH
MLEEMKRRQGRYGVASLCVGGGPAMATVIEMI
HHHHHHHHCCCCCCHHEEECCCHHHHHHHHHC
>Mature Secondary Structure
MEEAVIVGAARTAIGDFMGALKDVSAVDLGIAAVQGALKKAGIGPELVEEVVGGMIYKAG
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECC
AKGNPARQVQLKTGIPLEGAATTVEQLCGSAMRALEIASQQILLGKTSVGVAVGMESMSQ
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCHHHHCC
APYLLLNARQGLRLGSDSIEDHLLHDALIDAFSGTHMGITAENLAEKYGISREEQDDLAL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LSHQRAVAAAESGKFRAEIVPVEVKSRKGTIVIDTDEHPRADMSREKLAALRPAFKKDGT
HHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
VTAGNASSINDGAAALVLMTARRARELGVSPIARIRATASFGVAPEIMGIGPAYAIPKAL
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHH
KYADLQMKDIDYFEINEAFAAQFLAVNRELNIDLSRVNANGSGIALGHPVGCTGIRIIVT
HHCCCEECCCCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCHHHHHHHH
MLEEMKRRQGRYGVASLCVGGGPAMATVIEMI
HHHHHHHHCCCCCCHHEEECCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]