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The map label for this gene is pchF [H]

Identifier: 78223333

GI number: 78223333

Start: 2393500

End: 2395062

Strand: Direct

Name: pchF [H]

Synonym: Gmet_2126

Alternate gene names: 78223333

Gene position: 2393500-2395062 (Clockwise)

Preceding gene: 78223332

Following gene: 78223334

Centisome position: 59.88

GC content: 61.23

Gene sequence:

>1563_bases
ATGAGTCGTTACCTAGCGCTGCCAAAGGGCGTTACCGAAGCAGCCTTTACCAAAGCGATCACTGAGTATCGCACCCTTCT
GGGAGAGGGGCGCGTCCGCACCGATCTGGCATCCCTCAGTCCGTACATCGTCAACTCCATTGCCGAAAGCGATTCATTGC
ACACTCCCTCGGCAGCCATCTACCCGACGACCACCAGGGAAATCCAGTCCATTGTCGGCATTGCCAACAAGTACAAGACG
CCGCTCTGGACTGTCTGCAACGGGGAAAACGAGGGGTACGGCTCCTCGGCGCCGGCAACCGCCGGGCAGATAGTGCTCGA
TCTGCGAAACATGAAGAAGATCATCGAGGTCGATCGGGAGCTGGGCTACTGTCTGGTGGAGCCCGGCGTGACCTATGCCG
ACCTCCAGAACTACCTCACCAGCAACAAGATCAACCTCTGGCTCGATGCTCCGGCACCTTCGGCCAATGTCAGCATCCTC
GGCAATATCCTGGAGCGGGGGAGCGGCTACACCCCCTACAGCGAGGATTTCCTCTTTTCCTGCGGCATGGAGGTGGTGCT
GGCCAACGGGACTGTCCTCAAGACCGGCATGGGGGGCATCCCCAATTCCACCAGCTGGCAGGTCTTCAAGTGGGGGTTCG
GCCCCTATGTGGACGGGCTCTTTTCCCAGGGGAACAACGGGATCGTCACCAAGGTCGGCGCATGGCTGATGGGAGCACCG
CCTCCGGGTGGCTACAAGCCTTTCTGCATCAGGTTCCCCAAGAACGAGATGCTGCCCAACATCATCAAGCCGCTCATGTT
CCTGCGCCAGGCCCAGATCGTTCCCAACGCCTGCGCCCTGGTCAACGCCGGATGGGAAACGGCCACCCGTTTCGCCAATG
ACCGGAGGAACAACGGCCGTTTCATCAACACGGCGAACCGGGTCATTCCCGCCAACAAACTGGTCAACCGCAACGTTGGT
GCCTGGAATCTCTACGGGGCCGTCTACGGCTCTCCCGAGCAGGTGGCCCTGAACTGGATGTACGTCTCCGGCACCTTCAA
ACAGGCCTTCGGTAACAAGATCAGCATCATCACCGAGAATGAGGCCAAGGGGGACCCGGCCTTCGAGTATCGCAAGCAGC
TCATGATGGGGGGGGTCACCAGCCGCAATCCCCTGGACAGCTGGCGCCCCGGCTACGGCTCCATGAAGTTCGCCCCCACG
GCGGCGGCACGGCCGGGCGAATGCGCCCAGCAGGCGAAGGTGGCGGCAGAGATCATCAACCGGCACGGTTTCGACTACCT
CAGCGAGTTCAGCTTCTTCTGGTACGACGCCCGCCACACCGTCGATCTCCGTTTTGACCGCACCAGGCCCGAGGAGATGA
AACGGGCCCATGCCTGCTTCAACGAGCTCATCAGCGCCTTCGCCAAACATGGCTGGGCGGTGGGCCGCACCAACACCGCC
TTCATGGACAAGGTGGCCGATTCCTACGGCCCGGCAAAGCAGAGGCTCAACCGCACCATCAAGAAGGCCCTGGACCCCAA
CAACATCCTGTCGCCGGGCAAATCAGGGATCACGGCAGGGTAA

Upstream 100 bases:

>100_bases
TGAAGAAGGCGCTGGATCCCAACAATATCCTGGCCCCGGGCAAGTCGGGCATCAACCTGGATATTCCCCTGTAGCCGACA
ACCGTTGAGGAGGTAAGAAC

Downstream 100 bases:

>100_bases
CGTTCAATGAGCATTTGGAGGAAACAATCCATGAATCTATCAAAATCCCTGATACTGAGCATCTGCCTCACGACCATCGT
CTTCATCCACTCGGGAGCCT

Product: FAD linked oxidase-like

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSRYLALPKGVTEAAFTKAITEYRTLLGEGRVRTDLASLSPYIVNSIAESDSLHTPSAAIYPTTTREIQSIVGIANKYKT
PLWTVCNGENEGYGSSAPATAGQIVLDLRNMKKIIEVDRELGYCLVEPGVTYADLQNYLTSNKINLWLDAPAPSANVSIL
GNILERGSGYTPYSEDFLFSCGMEVVLANGTVLKTGMGGIPNSTSWQVFKWGFGPYVDGLFSQGNNGIVTKVGAWLMGAP
PPGGYKPFCIRFPKNEMLPNIIKPLMFLRQAQIVPNACALVNAGWETATRFANDRRNNGRFINTANRVIPANKLVNRNVG
AWNLYGAVYGSPEQVALNWMYVSGTFKQAFGNKISIITENEAKGDPAFEYRKQLMMGGVTSRNPLDSWRPGYGSMKFAPT
AAARPGECAQQAKVAAEIINRHGFDYLSEFSFFWYDARHTVDLRFDRTRPEEMKRAHACFNELISAFAKHGWAVGRTNTA
FMDKVADSYGPAKQRLNRTIKKALDPNNILSPGKSGITAG

Sequences:

>Translated_520_residues
MSRYLALPKGVTEAAFTKAITEYRTLLGEGRVRTDLASLSPYIVNSIAESDSLHTPSAAIYPTTTREIQSIVGIANKYKT
PLWTVCNGENEGYGSSAPATAGQIVLDLRNMKKIIEVDRELGYCLVEPGVTYADLQNYLTSNKINLWLDAPAPSANVSIL
GNILERGSGYTPYSEDFLFSCGMEVVLANGTVLKTGMGGIPNSTSWQVFKWGFGPYVDGLFSQGNNGIVTKVGAWLMGAP
PPGGYKPFCIRFPKNEMLPNIIKPLMFLRQAQIVPNACALVNAGWETATRFANDRRNNGRFINTANRVIPANKLVNRNVG
AWNLYGAVYGSPEQVALNWMYVSGTFKQAFGNKISIITENEAKGDPAFEYRKQLMMGGVTSRNPLDSWRPGYGSMKFAPT
AAARPGECAQQAKVAAEIINRHGFDYLSEFSFFWYDARHTVDLRFDRTRPEEMKRAHACFNELISAFAKHGWAVGRTNTA
FMDKVADSYGPAKQRLNRTIKKALDPNNILSPGKSGITAG
>Mature_519_residues
SRYLALPKGVTEAAFTKAITEYRTLLGEGRVRTDLASLSPYIVNSIAESDSLHTPSAAIYPTTTREIQSIVGIANKYKTP
LWTVCNGENEGYGSSAPATAGQIVLDLRNMKKIIEVDRELGYCLVEPGVTYADLQNYLTSNKINLWLDAPAPSANVSILG
NILERGSGYTPYSEDFLFSCGMEVVLANGTVLKTGMGGIPNSTSWQVFKWGFGPYVDGLFSQGNNGIVTKVGAWLMGAPP
PGGYKPFCIRFPKNEMLPNIIKPLMFLRQAQIVPNACALVNAGWETATRFANDRRNNGRFINTANRVIPANKLVNRNVGA
WNLYGAVYGSPEQVALNWMYVSGTFKQAFGNKISIITENEAKGDPAFEYRKQLMMGGVTSRNPLDSWRPGYGSMKFAPTA
AARPGECAQQAKVAAEIINRHGFDYLSEFSFFWYDARHTVDLRFDRTRPEEMKRAHACFNELISAFAKHGWAVGRTNTAF
MDKVADSYGPAKQRLNRTIKKALDPNNILSPGKSGITAG

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 57161; Mature: 57030

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYLALPKGVTEAAFTKAITEYRTLLGEGRVRTDLASLSPYIVNSIAESDSLHTPSAAI
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCEE
YPTTTREIQSIVGIANKYKTPLWTVCNGENEGYGSSAPATAGQIVLDLRNMKKIIEVDRE
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGYCLVEPGVTYADLQNYLTSNKINLWLDAPAPSANVSILGNILERGSGYTPYSEDFLFS
CCEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
CGMEVVLANGTVLKTGMGGIPNSTSWQVFKWGFGPYVDGLFSQGNNGIVTKVGAWLMGAP
CCCEEEEECCEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCEEECCC
PPGGYKPFCIRFPKNEMLPNIIKPLMFLRQAQIVPNACALVNAGWETATRFANDRRNNGR
CCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHCCCCCCC
FINTANRVIPANKLVNRNVGAWNLYGAVYGSPEQVALNWMYVSGTFKQAFGNKISIITEN
EEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCEEEEEEEEEECCHHHHCCCEEEEEECC
EAKGDPAFEYRKQLMMGGVTSRNPLDSWRPGYGSMKFAPTAAARPGECAQQAKVAAEIIN
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
RHGFDYLSEFSFFWYDARHTVDLRFDRTRPEEMKRAHACFNELISAFAKHGWAVGRTNTA
HCCHHHHHHCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCHH
FMDKVADSYGPAKQRLNRTIKKALDPNNILSPGKSGITAG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRYLALPKGVTEAAFTKAITEYRTLLGEGRVRTDLASLSPYIVNSIAESDSLHTPSAAI
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCEE
YPTTTREIQSIVGIANKYKTPLWTVCNGENEGYGSSAPATAGQIVLDLRNMKKIIEVDRE
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LGYCLVEPGVTYADLQNYLTSNKINLWLDAPAPSANVSILGNILERGSGYTPYSEDFLFS
CCEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
CGMEVVLANGTVLKTGMGGIPNSTSWQVFKWGFGPYVDGLFSQGNNGIVTKVGAWLMGAP
CCCEEEEECCEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCEEECCC
PPGGYKPFCIRFPKNEMLPNIIKPLMFLRQAQIVPNACALVNAGWETATRFANDRRNNGR
CCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHCCCCCCC
FINTANRVIPANKLVNRNVGAWNLYGAVYGSPEQVALNWMYVSGTFKQAFGNKISIITEN
EEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCEEEEEEEEEECCHHHHCCCEEEEEECC
EAKGDPAFEYRKQLMMGGVTSRNPLDSWRPGYGSMKFAPTAAARPGECAQQAKVAAEIIN
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
RHGFDYLSEFSFFWYDARHTVDLRFDRTRPEEMKRAHACFNELISAFAKHGWAVGRTNTA
HCCHHHHHHCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCHH
FMDKVADSYGPAKQRLNRTIKKALDPNNILSPGKSGITAG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]