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The map label for this gene is pchF [H]

Identifier: 78223332

GI number: 78223332

Start: 2391878

End: 2393473

Strand: Direct

Name: pchF [H]

Synonym: Gmet_2125

Alternate gene names: 78223332

Gene position: 2391878-2393473 (Clockwise)

Preceding gene: 78223331

Following gene: 78223333

Centisome position: 59.84

GC content: 61.47

Gene sequence:

>1596_bases
ATGAGTCGTTTCATTGCACTTCCCAAAGGAGTGACAGAAGCAGCCTTCGCCAAGGCCATCCAGGAATACCGGGCGCTCCT
GGGCGAGGACCGGGTCCGCACCGATCCGGCTTCCCTGCAGTCCTACATGAAGATCATGATCCCGGAGACCGAGGAACTGC
ACAAGCCTTCGGCCGCCATGTACCCCAGAACGGTCAAAGAGATCCAGGCCATCGTGGCCATAGCCAACAAGTACAAGACC
CCCCTCTGGACGGTGAGCACCGGCAAGAACATGGGATACGGTTCCGCTGCCCCGGCCACCCGGGGGCAGATCGTCCTCGA
CCTGAAGACCATGAAGAAGATCATCCATGTGGATGAGGAGCTGGGGTACTGCCTGGTGGAGCCGGGGGTGACCTACTACG
ACCTGCAGGAGTACTTCAGGAAGCACAAGATGAACCTCTGGGTCGATGTCCCCGCCCCCTCGTCCATGGCCAGCCCGATG
GGGAACACGGCAGACCGGGGGGTGGGCTACACCCCCTACGGCGAGCACTTCCTCGTTTCCTGCGGCATGGAGGTGGTCCT
GGCCAACGGCGACGTGGTCCGCACCGGCACGGGGGGCGTCCAGAACTCCACCAGCTGGCAGGCCAGCAAATGGGGCTACG
GACCGTATCTCGACGGCATTTTCACCCAGTCCAACTACGGCATCATCACCAAGCTCGGGGTCTGGCTCCTGAAGGGCCCT
CCTCCCGGCGGCTACATGCCGTTTCTCATGAAGTTCCCCAAGGTGGAGATGCTGGGCGACATCGTCAAGACCTTCATGCC
CCTGCGCCTGGCCCAGATCATCCCCAACGCCTGCGTCGTGGTCAACGCCGGCTGGGAGGCGGCCGGCTACTTCACCTACG
ACAAGAACGGCAAGGGAACCAACCGGAGCAGCTTCTACAAGGGGAAGGGAAGCCTCCCCCCCAAGGTGTTCCAGGAGATC
ATGGACAAGTTCAAGATCGGCGCCTGGAACTTCTACGCCGCCCTCTACGGCTCCCCCGAGCAGGTGGCCCTCAACTGGAA
ATACGTCTCGGGCGCCTTCAAGGCCAAGTTCGGCAATCAGGTCCAGATCATCACCGAGAAGGAAGCCAAGGACGACCCCA
TCTTCGAGTACCGCCGCCAGCTGATGATGGGGGGGAGCACCCTGCAGGAGTTCGGCCTCTACAACTGGCGTGGAGGGGGC
GGTTCCATGTGGTTCGCCCCGGTTGCGGCCGCCAGACCCTCCGAGTGCGACAAGCAGATGAGGGTTGCCACCGACATCCT
CAACAAGCACGGCTTCGACTACGTCTCCGAGTTCATTGTGGGGTGGCGCGAAATGCACCACATCATCGACCTCCTCTACG
ACCGTACCCAGCCGGACGAGATGCACCGGGCCTACCAGTGCTTCGATGAGTTGTTGAGGGTGTTTTCCAAGAACGGTTAC
GGGACCTACCGCACCAACACGGCCTTCATGGAAAAGGCTGCCAACACCTACGGGCCCGAGATGCGGAAGCTTCATCGGAC
CCTGAAGAAGGCGCTGGATCCCAACAATATCCTGGCCCCGGGCAAGTCGGGCATCAACCTGGATATTCCCCTGTAG

Upstream 100 bases:

>100_bases
GAAGCGATCGTCGATTGCGGCGGCAGCTTCCACTACACCACCTACGAAGACCGGAACCGGGTCACGTTTTCCGCACAGCT
GTAACCGAGGAGGTTTGAAA

Downstream 100 bases:

>100_bases
CCGACAACCGTTGAGGAGGTAAGAACATGAGTCGTTACCTAGCGCTGCCAAAGGGCGTTACCGAAGCAGCCTTTACCAAA
GCGATCACTGAGTATCGCAC

Product: 4-cresol dehydrogenase (hydroxylating)

Products: NA

Alternate protein names: P-cresol methylhydroxylase; PCMH [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSRFIALPKGVTEAAFAKAIQEYRALLGEDRVRTDPASLQSYMKIMIPETEELHKPSAAMYPRTVKEIQAIVAIANKYKT
PLWTVSTGKNMGYGSAAPATRGQIVLDLKTMKKIIHVDEELGYCLVEPGVTYYDLQEYFRKHKMNLWVDVPAPSSMASPM
GNTADRGVGYTPYGEHFLVSCGMEVVLANGDVVRTGTGGVQNSTSWQASKWGYGPYLDGIFTQSNYGIITKLGVWLLKGP
PPGGYMPFLMKFPKVEMLGDIVKTFMPLRLAQIIPNACVVVNAGWEAAGYFTYDKNGKGTNRSSFYKGKGSLPPKVFQEI
MDKFKIGAWNFYAALYGSPEQVALNWKYVSGAFKAKFGNQVQIITEKEAKDDPIFEYRRQLMMGGSTLQEFGLYNWRGGG
GSMWFAPVAAARPSECDKQMRVATDILNKHGFDYVSEFIVGWREMHHIIDLLYDRTQPDEMHRAYQCFDELLRVFSKNGY
GTYRTNTAFMEKAANTYGPEMRKLHRTLKKALDPNNILAPGKSGINLDIPL

Sequences:

>Translated_531_residues
MSRFIALPKGVTEAAFAKAIQEYRALLGEDRVRTDPASLQSYMKIMIPETEELHKPSAAMYPRTVKEIQAIVAIANKYKT
PLWTVSTGKNMGYGSAAPATRGQIVLDLKTMKKIIHVDEELGYCLVEPGVTYYDLQEYFRKHKMNLWVDVPAPSSMASPM
GNTADRGVGYTPYGEHFLVSCGMEVVLANGDVVRTGTGGVQNSTSWQASKWGYGPYLDGIFTQSNYGIITKLGVWLLKGP
PPGGYMPFLMKFPKVEMLGDIVKTFMPLRLAQIIPNACVVVNAGWEAAGYFTYDKNGKGTNRSSFYKGKGSLPPKVFQEI
MDKFKIGAWNFYAALYGSPEQVALNWKYVSGAFKAKFGNQVQIITEKEAKDDPIFEYRRQLMMGGSTLQEFGLYNWRGGG
GSMWFAPVAAARPSECDKQMRVATDILNKHGFDYVSEFIVGWREMHHIIDLLYDRTQPDEMHRAYQCFDELLRVFSKNGY
GTYRTNTAFMEKAANTYGPEMRKLHRTLKKALDPNNILAPGKSGINLDIPL
>Mature_530_residues
SRFIALPKGVTEAAFAKAIQEYRALLGEDRVRTDPASLQSYMKIMIPETEELHKPSAAMYPRTVKEIQAIVAIANKYKTP
LWTVSTGKNMGYGSAAPATRGQIVLDLKTMKKIIHVDEELGYCLVEPGVTYYDLQEYFRKHKMNLWVDVPAPSSMASPMG
NTADRGVGYTPYGEHFLVSCGMEVVLANGDVVRTGTGGVQNSTSWQASKWGYGPYLDGIFTQSNYGIITKLGVWLLKGPP
PGGYMPFLMKFPKVEMLGDIVKTFMPLRLAQIIPNACVVVNAGWEAAGYFTYDKNGKGTNRSSFYKGKGSLPPKVFQEIM
DKFKIGAWNFYAALYGSPEQVALNWKYVSGAFKAKFGNQVQIITEKEAKDDPIFEYRRQLMMGGSTLQEFGLYNWRGGGG
SMWFAPVAAARPSECDKQMRVATDILNKHGFDYVSEFIVGWREMHHIIDLLYDRTQPDEMHRAYQCFDELLRVFSKNGYG
TYRTNTAFMEKAANTYGPEMRKLHRTLKKALDPNNILAPGKSGINLDIPL

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR006094
- InterPro:   IPR016170
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: =1.17.99.1 [H]

Molecular weight: Translated: 59503; Mature: 59372

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFIALPKGVTEAAFAKAIQEYRALLGEDRVRTDPASLQSYMKIMIPETEELHKPSAAM
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHC
YPRTVKEIQAIVAIANKYKTPLWTVSTGKNMGYGSAAPATRGQIVLDLKTMKKIIHVDEE
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
LGYCLVEPGVTYYDLQEYFRKHKMNLWVDVPAPSSMASPMGNTADRGVGYTPYGEHFLVS
CCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCEEEE
CGMEVVLANGDVVRTGTGGVQNSTSWQASKWGYGPYLDGIFTQSNYGIITKLGVWLLKGP
CCEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEECEEEEECC
PPGGYMPFLMKFPKVEMLGDIVKTFMPLRLAQIIPNACVVVNAGWEAAGYFTYDKNGKGT
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCC
NRSSFYKGKGSLPPKVFQEIMDKFKIGAWNFYAALYGSPEQVALNWKYVSGAFKAKFGNQ
CCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEHEEECCCCEEEEEEEEECCHHHHCCCCE
VQIITEKEAKDDPIFEYRRQLMMGGSTLQEFGLYNWRGGGGSMWFAPVAAARPSECDKQM
EEEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCEEECCCCCEEECCCCCCCCHHHHHHH
RVATDILNKHGFDYVSEFIVGWREMHHIIDLLYDRTQPDEMHRAYQCFDELLRVFSKNGY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
GTYRTNTAFMEKAANTYGPEMRKLHRTLKKALDPNNILAPGKSGINLDIPL
CEEECCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCC
>Mature Secondary Structure 
SRFIALPKGVTEAAFAKAIQEYRALLGEDRVRTDPASLQSYMKIMIPETEELHKPSAAM
CCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHC
YPRTVKEIQAIVAIANKYKTPLWTVSTGKNMGYGSAAPATRGQIVLDLKTMKKIIHVDEE
CHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
LGYCLVEPGVTYYDLQEYFRKHKMNLWVDVPAPSSMASPMGNTADRGVGYTPYGEHFLVS
CCEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCEEEE
CGMEVVLANGDVVRTGTGGVQNSTSWQASKWGYGPYLDGIFTQSNYGIITKLGVWLLKGP
CCEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEECEEEEECC
PPGGYMPFLMKFPKVEMLGDIVKTFMPLRLAQIIPNACVVVNAGWEAAGYFTYDKNGKGT
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCC
NRSSFYKGKGSLPPKVFQEIMDKFKIGAWNFYAALYGSPEQVALNWKYVSGAFKAKFGNQ
CCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEHEEECCCCEEEEEEEEECCHHHHCCCCE
VQIITEKEAKDDPIFEYRRQLMMGGSTLQEFGLYNWRGGGGSMWFAPVAAARPSECDKQM
EEEEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCEEECCCCCEEECCCCCCCCHHHHHHH
RVATDILNKHGFDYVSEFIVGWREMHHIIDLLYDRTQPDEMHRAYQCFDELLRVFSKNGY
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
GTYRTNTAFMEKAANTYGPEMRKLHRTLKKALDPNNILAPGKSGINLDIPL
CEEECCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7929007; 10565539; 3790500; 1846290; 10623531 [H]