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The map label for this gene is paaK [H]

Identifier: 78223214

GI number: 78223214

Start: 2245505

End: 2246968

Strand: Reverse

Name: paaK [H]

Synonym: Gmet_2007

Alternate gene names: 78223214

Gene position: 2246968-2245505 (Counterclockwise)

Preceding gene: 78223215

Following gene: 78223213

Centisome position: 56.21

GC content: 56.63

Gene sequence:

>1464_bases
ATGCAGGTATACCCTCTGATCATGAACAAGCTCGTCATGCCGGCCGCATCGCGGTTTACGGACCTCAAAATATGGGACGA
GTACAAGGTGATGCTGAAATCGGAACGGCTGAGCCTTGAACAGCTGCGCCAGATGCAGTTGACGAAGCTTCAGACGATTG
TCCGCCATGCCTACGAGAATGTCCCGTTTTACCGGCAGCGGTTCGATTCGATCGGGCTGCATCCCGAAGATATCAAGTCC
ATTGAGGACATCGAGCGGATTCCGCCGACCACCAAGGACGACATCATGGCCAACTTCCCCGAGGGGATTACGGCCGGGGG
GATGGACCGCAACCGCTGGAAGTATGTGGCCAGTTCAGGCACGACGCGCAAGATCATGGGTATCCACGATTTTCGCAAGT
CGACCCTCAACTGGGCCGCGGGGATCAGGGCCCACCGTCTTGCCGGCAACCACAGGATGGGGATGAAGTGGATGGAGATT
CCTCCTCACATGTGCACCACCATCTGCGGGATCAATGACGGGCCCAAGGGTGAGAAACTCTCCACGAAGAAGCTGGTTTC
ACTGGTTAAGGAAGGTGATTTCGGCGGTGCGGGGAGGCATGTGTACGAGGGGCTCTACAGCATGCGTCATGACCTGTACA
AGCGGGTGACGCTCCCTTCCTTCGGTCCGGAGGGGACCAATATCCCGGAAAGCGACATTGTTTCCTATCTGGATTCTATC
CGGAGCTATCAGCCACACCTGCTGGAGGGGCTTCCTCTCTATTTGTACACGTTTGCCAAGTACATTCTCGACAAGGGGAT
TTCCGCTCCCCGCGTCGGTGTCATCAAGCCCTTCGGCGGTTCGTTGACCCCGTACATGAAACAGATTATCGCCACGGCGT
TCGGGTGCGAGGTGTATGACACTTACGGCTGCAGCGAGTTGGGTTTCATGGCCGCTGATTGTCCGAAGCACGAAGGGGTC
CATCTCTTCATGGATCTCTACCATGTGGAGGTCCGTCATGGTGATCGGGCCGCAGCACCGGGGGAACTGGGCAAGTTCTA
CGTGACTGATCTGGAAAACAGGGCCATGCCGTGGATCAGATACGAGATCGGGGATGTTGGCCGCTACTTCGAGGATGACC
ACGGATGCGGCCGGAGAGGGATCCGGCTGGAAGTGGAGGGAAGGGTGCAGGACACCTTGGTCAACTCCCAGGGGAGGTTT
TTCACCAGCGACGAGGTGTTCGATTTTTTCCATCGGCGTGAAGAGATCGACAACTTCCAGCTAACGGAGAAATCGGCCGG
CCGGTATGAGCTGCTGTGTGTTCCGGTTGAAGGTAAAGAGGTCGACCGGGAGGGGATTGCCAGGGATTTCCGGGAATTCT
TCGATACTGAGGCGAATGTAAAGGCATACGCGGTAAAGACCATCAAGGCCGAGGATGGCGGGAAGTTCCGGTTTATCAAG
GCAAAGGGGCACGACAGGCTGTAA

Upstream 100 bases:

>100_bases
GAAAAAAGGCACAAACTCTCCGAACCTTACTTTGTTTTTCGGGTTTCAATTTCGTGCTTTCGTGATCAAAGCCTTTATGA
AGTAGAAAAGGAGTAGAGCC

Downstream 100 bases:

>100_bases
TGCTGAAGTAGTGAAGGAGATTTTTCGATGGACATTCAGAAAATCAGGGGAGATTTCCCCATCTTTGAGAGGCGCATCAA
CGGGAAGCCGATAGTCTATT

Product: CapK related-protein

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MQVYPLIMNKLVMPAASRFTDLKIWDEYKVMLKSERLSLEQLRQMQLTKLQTIVRHAYENVPFYRQRFDSIGLHPEDIKS
IEDIERIPPTTKDDIMANFPEGITAGGMDRNRWKYVASSGTTRKIMGIHDFRKSTLNWAAGIRAHRLAGNHRMGMKWMEI
PPHMCTTICGINDGPKGEKLSTKKLVSLVKEGDFGGAGRHVYEGLYSMRHDLYKRVTLPSFGPEGTNIPESDIVSYLDSI
RSYQPHLLEGLPLYLYTFAKYILDKGISAPRVGVIKPFGGSLTPYMKQIIATAFGCEVYDTYGCSELGFMAADCPKHEGV
HLFMDLYHVEVRHGDRAAAPGELGKFYVTDLENRAMPWIRYEIGDVGRYFEDDHGCGRRGIRLEVEGRVQDTLVNSQGRF
FTSDEVFDFFHRREEIDNFQLTEKSAGRYELLCVPVEGKEVDREGIARDFREFFDTEANVKAYAVKTIKAEDGGKFRFIK
AKGHDRL

Sequences:

>Translated_487_residues
MQVYPLIMNKLVMPAASRFTDLKIWDEYKVMLKSERLSLEQLRQMQLTKLQTIVRHAYENVPFYRQRFDSIGLHPEDIKS
IEDIERIPPTTKDDIMANFPEGITAGGMDRNRWKYVASSGTTRKIMGIHDFRKSTLNWAAGIRAHRLAGNHRMGMKWMEI
PPHMCTTICGINDGPKGEKLSTKKLVSLVKEGDFGGAGRHVYEGLYSMRHDLYKRVTLPSFGPEGTNIPESDIVSYLDSI
RSYQPHLLEGLPLYLYTFAKYILDKGISAPRVGVIKPFGGSLTPYMKQIIATAFGCEVYDTYGCSELGFMAADCPKHEGV
HLFMDLYHVEVRHGDRAAAPGELGKFYVTDLENRAMPWIRYEIGDVGRYFEDDHGCGRRGIRLEVEGRVQDTLVNSQGRF
FTSDEVFDFFHRREEIDNFQLTEKSAGRYELLCVPVEGKEVDREGIARDFREFFDTEANVKAYAVKTIKAEDGGKFRFIK
AKGHDRL
>Mature_487_residues
MQVYPLIMNKLVMPAASRFTDLKIWDEYKVMLKSERLSLEQLRQMQLTKLQTIVRHAYENVPFYRQRFDSIGLHPEDIKS
IEDIERIPPTTKDDIMANFPEGITAGGMDRNRWKYVASSGTTRKIMGIHDFRKSTLNWAAGIRAHRLAGNHRMGMKWMEI
PPHMCTTICGINDGPKGEKLSTKKLVSLVKEGDFGGAGRHVYEGLYSMRHDLYKRVTLPSFGPEGTNIPESDIVSYLDSI
RSYQPHLLEGLPLYLYTFAKYILDKGISAPRVGVIKPFGGSLTPYMKQIIATAFGCEVYDTYGCSELGFMAADCPKHEGV
HLFMDLYHVEVRHGDRAAAPGELGKFYVTDLENRAMPWIRYEIGDVGRYFEDDHGCGRRGIRLEVEGRVQDTLVNSQGRF
FTSDEVFDFFHRREEIDNFQLTEKSAGRYELLCVPVEGKEVDREGIARDFREFFDTEANVKAYAVKTIKAEDGGKFRFIK
AKGHDRL

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=426, Percent_Identity=27.9342723004695, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 55677; Mature: 55677

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVYPLIMNKLVMPAASRFTDLKIWDEYKVMLKSERLSLEQLRQMQLTKLQTIVRHAYEN
CEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
VPFYRQRFDSIGLHPEDIKSIEDIERIPPTTKDDIMANFPEGITAGGMDRNRWKYVASSG
CCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCC
TTRKIMGIHDFRKSTLNWAAGIRAHRLAGNHRMGMKWMEIPPHMCTTICGINDGPKGEKL
CCEEEECHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
STKKLVSLVKEGDFGGAGRHVYEGLYSMRHDLYKRVTLPSFGPEGTNIPESDIVSYLDSI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RSYQPHLLEGLPLYLYTFAKYILDKGISAPRVGVIKPFGGSLTPYMKQIIATAFGCEVYD
HCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCC
TYGCSELGFMAADCPKHEGVHLFMDLYHVEVRHGDRAAAPGELGKFYVTDLENRAMPWIR
CCCCCHHCCEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEE
YEIGDVGRYFEDDHGCGRRGIRLEVEGRVQDTLVNSQGRFFTSDEVFDFFHRREEIDNFQ
EEHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCE
LTEKSAGRYELLCVPVEGKEVDREGIARDFREFFDTEANVKAYAVKTIKAEDGGKFRFIK
EECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEEE
AKGHDRL
CCCCCCC
>Mature Secondary Structure
MQVYPLIMNKLVMPAASRFTDLKIWDEYKVMLKSERLSLEQLRQMQLTKLQTIVRHAYEN
CEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
VPFYRQRFDSIGLHPEDIKSIEDIERIPPTTKDDIMANFPEGITAGGMDRNRWKYVASSG
CCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEECCC
TTRKIMGIHDFRKSTLNWAAGIRAHRLAGNHRMGMKWMEIPPHMCTTICGINDGPKGEKL
CCEEEECHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
STKKLVSLVKEGDFGGAGRHVYEGLYSMRHDLYKRVTLPSFGPEGTNIPESDIVSYLDSI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
RSYQPHLLEGLPLYLYTFAKYILDKGISAPRVGVIKPFGGSLTPYMKQIIATAFGCEVYD
HCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCC
TYGCSELGFMAADCPKHEGVHLFMDLYHVEVRHGDRAAAPGELGKFYVTDLENRAMPWIR
CCCCCHHCCEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCEEE
YEIGDVGRYFEDDHGCGRRGIRLEVEGRVQDTLVNSQGRFFTSDEVFDFFHRREEIDNFQ
EEHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHCCCCCCEECCHHHHHHHHHHHHCCCCE
LTEKSAGRYELLCVPVEGKEVDREGIARDFREFFDTEANVKAYAVKTIKAEDGGKFRFIK
EECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEEEEE
AKGHDRL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]