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The map label for this gene is icaA [H]

Identifier: 78223215

GI number: 78223215

Start: 2247131

End: 2248270

Strand: Reverse

Name: icaA [H]

Synonym: Gmet_2008

Alternate gene names: 78223215

Gene position: 2248270-2247131 (Counterclockwise)

Preceding gene: 78223216

Following gene: 78223214

Centisome position: 56.24

GC content: 58.42

Gene sequence:

>1140_bases
ATGAGTGAATGGATTTTCTGGTTGCTGGTTGTCACGATTTTCTATACCTACTTCGGCTACGCGCTGCTGATCCCCCTCAT
CGGCCTGTTTGTGAAACGGCCTCTGGACCGGCGCGACATAACGCCTCGTGTAACCTTCCTGATCACCGCCTACAACGAGG
AGAAGAATATCCGCAAGAAGCTGACGGAAGTACTTGCGCTCGACTATCCCTGGGAACAGCTTGAGGTGATGGTGGCTTCC
GATGGCTCCACCGACCGCACTGACGACATCGTGCGCGAGTTCCGCGATCAGGGGGTGGTTCTGAAGCGGGTCGAGGGGAG
GGTTGGGAAGACCGCCACCCAGAATGAGGCGGTTAAGGCGGCCACCGGCGATGTCATCATCTTCTCCGACGCCACGACCG
TCTACGAGAAGGACGCCATCCGCAAGCTGGTCCGCAACTACAACGACCCTGAAGTGGGGGCCGTGAGCGGCCGCTACGAG
TACGTGAACCCCACGGGGGCACCGGTGGGGACCGGCTCGATCCTTTTCTGGAAGTACGAGAACTTCATCAAAAGCATGCA
GACCCGGATCAGGACCATCTCGGGGTGCTGCGGTTGCATCTACTCGGTGCGCAAGGCGGCCTATGTGCCGCTTCCCGGCG
ACATCATCAGCGATCTGGTGGAGCCCCTCATGGTGATCCGGGGCGGGTACCGTGTTGTCTTCGAGCAGGAGGCAGTGGCC
TACGAAGAGACCACCGAGAAGTCCCACGAGGAGTTCCGGATGCGCATCCGGGTCATCACCCGGGCCATGCGGGGCATCCT
CTTCGCCCGGGGGCTTCTGAACCCTTTTCGTTTTCCGTTTGTTGCCTTTCAGCTCTTTTCCCACAAAGTGCTCCGGTGGC
TGATTCCGTTCTTCCTGATCGGGCTGCTGGTCGCTAATGCATTTCTGCTCGATAGGCAGTTCTACGTGATTACCTTTGTG
CTTCAGGTGGCGTTCTATCTGCTGGCGTTGTTTGGTCTCGGGGCTGACCGGGTGGGTAAGAAGTTCAAGCCCGCCGCGGT
GCCGCTCTATTTCTGCATCGTGAATACGGCGTCGCTTATTGCTTTTTTCAAAACCCTTGCTGGGCAGAAGATGGTTACGT
GGGAGACGGTGCGCAAGTGA

Upstream 100 bases:

>100_bases
TTGACCAGCACAACGCTCGGATTGCGGCTTCTGTGACTGACGTCGTGGCTGTTCCCGCTGCCCCTGAAATGGACCATTTC
CCGAACAGTAGGATTGCGTC

Downstream 100 bases:

>100_bases
CTTGAACACACGAATAAAGGCAAATAAGAACTAATCACGAAAGCACGAAAGGTCGAAAGCACGAAAAAAGGCACAAACTC
TCCGAACCTTACTTTGTTTT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSEWIFWLLVVTIFYTYFGYALLIPLIGLFVKRPLDRRDITPRVTFLITAYNEEKNIRKKLTEVLALDYPWEQLEVMVAS
DGSTDRTDDIVREFRDQGVVLKRVEGRVGKTATQNEAVKAATGDVIIFSDATTVYEKDAIRKLVRNYNDPEVGAVSGRYE
YVNPTGAPVGTGSILFWKYENFIKSMQTRIRTISGCCGCIYSVRKAAYVPLPGDIISDLVEPLMVIRGGYRVVFEQEAVA
YEETTEKSHEEFRMRIRVITRAMRGILFARGLLNPFRFPFVAFQLFSHKVLRWLIPFFLIGLLVANAFLLDRQFYVITFV
LQVAFYLLALFGLGADRVGKKFKPAAVPLYFCIVNTASLIAFFKTLAGQKMVTWETVRK

Sequences:

>Translated_379_residues
MSEWIFWLLVVTIFYTYFGYALLIPLIGLFVKRPLDRRDITPRVTFLITAYNEEKNIRKKLTEVLALDYPWEQLEVMVAS
DGSTDRTDDIVREFRDQGVVLKRVEGRVGKTATQNEAVKAATGDVIIFSDATTVYEKDAIRKLVRNYNDPEVGAVSGRYE
YVNPTGAPVGTGSILFWKYENFIKSMQTRIRTISGCCGCIYSVRKAAYVPLPGDIISDLVEPLMVIRGGYRVVFEQEAVA
YEETTEKSHEEFRMRIRVITRAMRGILFARGLLNPFRFPFVAFQLFSHKVLRWLIPFFLIGLLVANAFLLDRQFYVITFV
LQVAFYLLALFGLGADRVGKKFKPAAVPLYFCIVNTASLIAFFKTLAGQKMVTWETVRK
>Mature_378_residues
SEWIFWLLVVTIFYTYFGYALLIPLIGLFVKRPLDRRDITPRVTFLITAYNEEKNIRKKLTEVLALDYPWEQLEVMVASD
GSTDRTDDIVREFRDQGVVLKRVEGRVGKTATQNEAVKAATGDVIIFSDATTVYEKDAIRKLVRNYNDPEVGAVSGRYEY
VNPTGAPVGTGSILFWKYENFIKSMQTRIRTISGCCGCIYSVRKAAYVPLPGDIISDLVEPLMVIRGGYRVVFEQEAVAY
EETTEKSHEEFRMRIRVITRAMRGILFARGLLNPFRFPFVAFQLFSHKVLRWLIPFFLIGLLVANAFLLDRQFYVITFVL
QVAFYLLALFGLGADRVGKKFKPAAVPLYFCIVNTASLIAFFKTLAGQKMVTWETVRK

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 43360; Mature: 43229

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWIFWLLVVTIFYTYFGYALLIPLIGLFVKRPLDRRDITPRVTFLITAYNEEKNIRKK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHH
LTEVLALDYPWEQLEVMVASDGSTDRTDDIVREFRDQGVVLKRVEGRVGKTATQNEAVKA
HHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEHHHCCCCCCCCCCCCHHHC
ATGDVIIFSDATTVYEKDAIRKLVRNYNDPEVGAVSGRYEYVNPTGAPVGTGSILFWKYE
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEHHH
NFIKSMQTRIRTISGCCGCIYSVRKAAYVPLPGDIISDLVEPLMVIRGGYRVVFEQEAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHH
YEETTEKSHEEFRMRIRVITRAMRGILFARGLLNPFRFPFVAFQLFSHKVLRWLIPFFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLVANAFLLDRQFYVITFVLQVAFYLLALFGLGADRVGKKFKPAAVPLYFCIVNTASLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
AFFKTLAGQKMVTWETVRK
HHHHHHCCCCEEHHHHHCC
>Mature Secondary Structure 
SEWIFWLLVVTIFYTYFGYALLIPLIGLFVKRPLDRRDITPRVTFLITAYNEEKNIRKK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHH
LTEVLALDYPWEQLEVMVASDGSTDRTDDIVREFRDQGVVLKRVEGRVGKTATQNEAVKA
HHHHHHCCCCHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEHHHCCCCCCCCCCCCHHHC
ATGDVIIFSDATTVYEKDAIRKLVRNYNDPEVGAVSGRYEYVNPTGAPVGTGSILFWKYE
CCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEHHH
NFIKSMQTRIRTISGCCGCIYSVRKAAYVPLPGDIISDLVEPLMVIRGGYRVVFEQEAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHH
YEETTEKSHEEFRMRIRVITRAMRGILFARGLLNPFRFPFVAFQLFSHKVLRWLIPFFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLVANAFLLDRQFYVITFVLQVAFYLLALFGLGADRVGKKFKPAAVPLYFCIVNTASLI
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHH
AFFKTLAGQKMVTWETVRK
HHHHHHCCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]