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The map label for this gene is hydN [H]

Identifier: 78222964

GI number: 78222964

Start: 1970563

End: 1971012

Strand: Reverse

Name: hydN [H]

Synonym: Gmet_1755

Alternate gene names: 78222964

Gene position: 1971012-1970563 (Counterclockwise)

Preceding gene: 78222965

Following gene: 78222963

Centisome position: 49.31

GC content: 63.78

Gene sequence:

>450_bases
ATGAAGCGCATCTACATGATCGAAGACGTCTGCATCGGCTGCCACCTCTGTGAGGTGGCGTGCATCACCGAGCACTCCCA
GTCCAAAAACCCGGTCAAGGCATTTCTCCACGAGGAGTACCGCCCCATTTCCCGCTGCACCGTCGAGGAGTGGGACGGAG
GAGTCGTCTCCTTCTCCACCACCTGCCGCCACTGCGACGAGCCCGACTGCCTGCGGGCCTGCATCTCCGGCGCCATCCAG
AAAGACGACAAGGGCGTGGTCCGGATCGACACCGAACAGTGCGTCGGCTGCTGGTCCTGCGTCATGGCCTGCCCCTACGG
CGCCGTGCAGCGCAACCTCACGAAGAAAAAGGCCAACAAGTGCGACCTCTGCCCGGACCGCACCAGCCCGGCCTGCGTCG
ATGCCTGCCCCAACCGGGCACTCGTCTACAAGGAGGGGAGCCAGAAATGA

Upstream 100 bases:

>100_bases
CCTGGAGTCGCTGCGGGGCAACCGTTACAAGCTCCGCGCCCTTGGCCTCACCGAGAAAGACATGAACATCCTGGGCGTCA
TGCCCGCTGGAGAATAAGGT

Downstream 100 bases:

>100_bases
ACTATGTGATCATTGGAAACTCCGTGGCTGCCGTGGGCGCCATCCGGGGTATCAGGAGCATCGACCAGCAGGGGAACATC
ACCGTCATCTCGCGGGAGCG

Product: Iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKRIYMIEDVCIGCHLCEVACITEHSQSKNPVKAFLHEEYRPISRCTVEEWDGGVVSFSTTCRHCDEPDCLRACISGAIQ
KDDKGVVRIDTEQCVGCWSCVMACPYGAVQRNLTKKKANKCDLCPDRTSPACVDACPNRALVYKEGSQK

Sequences:

>Translated_149_residues
MKRIYMIEDVCIGCHLCEVACITEHSQSKNPVKAFLHEEYRPISRCTVEEWDGGVVSFSTTCRHCDEPDCLRACISGAIQ
KDDKGVVRIDTEQCVGCWSCVMACPYGAVQRNLTKKKANKCDLCPDRTSPACVDACPNRALVYKEGSQK
>Mature_149_residues
MKRIYMIEDVCIGCHLCEVACITEHSQSKNPVKAFLHEEYRPISRCTVEEWDGGVVSFSTTCRHCDEPDCLRACISGAIQ
KDDKGVVRIDTEQCVGCWSCVMACPYGAVQRNLTKKKANKCDLCPDRTSPACVDACPNRALVYKEGSQK

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=156, Percent_Identity=38.4615384615385, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=153, Percent_Identity=37.9084967320261, Blast_Score=92, Evalue=1e-20,
Organism=Escherichia coli, GI1787872, Length=153, Percent_Identity=37.2549019607843, Blast_Score=90, Evalue=6e-20,
Organism=Escherichia coli, GI1788811, Length=150, Percent_Identity=36.6666666666667, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI87082180, Length=135, Percent_Identity=37.037037037037, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=149, Percent_Identity=34.2281879194631, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI87082179, Length=148, Percent_Identity=36.4864864864865, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI2367245, Length=145, Percent_Identity=38.6206896551724, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1787749, Length=150, Percent_Identity=34, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1790326, Length=153, Percent_Identity=32.6797385620915, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367345, Length=149, Percent_Identity=34.2281879194631, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1787963, Length=140, Percent_Identity=32.1428571428571, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1789370, Length=159, Percent_Identity=25.7861635220126, Blast_Score=63, Evalue=6e-12,
Organism=Escherichia coli, GI1789079, Length=174, Percent_Identity=29.8850574712644, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 16599; Mature: 16599

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
13.4 %Cys+Met (Translated Protein)
11.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
13.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIYMIEDVCIGCHLCEVACITEHSQSKNPVKAFLHEEYRPISRCTVEEWDGGVVSFST
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEEEC
TCRHCDEPDCLRACISGAIQKDDKGVVRIDTEQCVGCWSCVMACPYGAVQRNLTKKKANK
HHCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
CDLCPDRTSPACVDACPNRALVYKEGSQK
CCCCCCCCCCHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MKRIYMIEDVCIGCHLCEVACITEHSQSKNPVKAFLHEEYRPISRCTVEEWDGGVVSFST
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEEEC
TCRHCDEPDCLRACISGAIQKDDKGVVRIDTEQCVGCWSCVMACPYGAVQRNLTKKKANK
HHCCCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
CDLCPDRTSPACVDACPNRALVYKEGSQK
CCCCCCCCCCHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]