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The map label for this gene is nasD [H]

Identifier: 78222963

GI number: 78222963

Start: 1969256

End: 1970566

Strand: Reverse

Name: nasD [H]

Synonym: Gmet_1754

Alternate gene names: 78222963

Gene position: 1970566-1969256 (Counterclockwise)

Preceding gene: 78222964

Following gene: 78222962

Centisome position: 49.3

GC content: 62.01

Gene sequence:

>1311_bases
ATGAACTATGTGATCATTGGAAACTCCGTGGCTGCCGTGGGCGCCATCCGGGGTATCAGGAGCATCGACCAGCAGGGGAA
CATCACCGTCATCTCGCGGGAGCGCCACGTAGCCTACGGCCGCCCCCTCATCTCCTACCTCCTCGGCGGCCTCATCACGG
AAAAGCGGATGGCCTACCTCCCGGAGGACTTCTACGAGAAGAACCGGGTGAACCTCCTCCTCAACGCGGAAGTGGTTGGC
GTCGACACCGACAACCGGAAAGTGACCATCGCCGGCGGCGATGTGATCGTCTACGACAAGCTCCTCGTTGCGACGGGAGG
CGACCCTTTCGTTCCTCCCATCGAGGGAATGGCCGGCAAGGACCGGATCTTCACCTTCACCACCTGGGACGACGCGGCGA
AACTCAAGGGGATTGCCGACGATATCGAGAAGGTAGTGGTCATCGGCGGCGGCCTCATCGGCCTCAAGGCTGCAGAAGGA
CTCCATCTTATCGATAAAAAAATCACCATCGTGGAACTGGCCGACCGGATCCTCTCCGCCGCCTTTGACCGCCCCGCCGG
GCGGGTAGTGGCCAAAAAGATGAAGGCCAACGGCATCGACGTCATCACCGAGGACACGGTGGTGCGGATCGAGGGTGATG
GCGCCGCCATCACCGGCGTCACCCTGAAGTCGGGCGACTTTATCCCCTGCGACACGGTTGTCGTCGCCATCGGGGTGCGC
CCCGCAGCCGGATTCCTCAAGGGAAGCGGCGTGGAGGTGAACCGGGGCATCGTGGTGGACGACCTGATGGAGACCAACGT
GAAGGGGGTCTTCGCCGCTGGCGACGTGGCCGAGGCAAAGGACTTCTTCTCCGGCGTCAAGAACCCGATGCCCATCTGGC
CCGACGCCTACATCCAGGGGGACATCGCCGGGGTAACCATGGCGGGCGGTTCAAAGGGGTACCAAGGCGGCATTGCCATG
AACTCCATCGAACTCTTCAAGGTATCCACCATCTCCATGGGGATCACGAACCCGGTGGAGCCCAAGGAATACGAGATCCT
CACCTATCAGGATCTGGAGAACTACCAGTACCGAAAGATCGTCCTCAAGGACGGTATCCTGGCTGGCGCCGTCCTCGTGG
GGGCCGTGGACCGGGCCGGCATCTTTGCCGGTCTTATCCGCGACCGGATCGAGGTAAGCTCTTTCAAGGACCACCTCCTC
ACCCCCGACTTCGGCTTCGTCAATCTCCCCCGGGAGATTCGCTCCACCCTCTTCGCCCCCGTCGGCAAAGTGACGGGAGC
TTACGAACAGGCCAGGGCCGTCGGGCATTGA

Upstream 100 bases:

>100_bases
AAAAGGCCAACAAGTGCGACCTCTGCCCGGACCGCACCAGCCCGGCCTGCGTCGATGCCTGCCCCAACCGGGCACTCGTC
TACAAGGAGGGGAGCCAGAA

Downstream 100 bases:

>100_bases
CCCGGCACCGTTTCGCGACGACCAGATCGAGGTTTTGACATGACCAGAGAATTGAAACCGACCCACTACTTCCAGAAAGA
CATCTCCAACTGCGGCCTGA

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHVAYGRPLISYLLGGLITEKRMAYLPEDFYEKNRVNLLLNAEVVG
VDTDNRKVTIAGGDVIVYDKLLVATGGDPFVPPIEGMAGKDRIFTFTTWDDAAKLKGIADDIEKVVVIGGGLIGLKAAEG
LHLIDKKITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGDGAAITGVTLKSGDFIPCDTVVVAIGVR
PAAGFLKGSGVEVNRGIVVDDLMETNVKGVFAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVTMAGGSKGYQGGIAM
NSIELFKVSTISMGITNPVEPKEYEILTYQDLENYQYRKIVLKDGILAGAVLVGAVDRAGIFAGLIRDRIEVSSFKDHLL
TPDFGFVNLPREIRSTLFAPVGKVTGAYEQARAVGH

Sequences:

>Translated_436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHVAYGRPLISYLLGGLITEKRMAYLPEDFYEKNRVNLLLNAEVVG
VDTDNRKVTIAGGDVIVYDKLLVATGGDPFVPPIEGMAGKDRIFTFTTWDDAAKLKGIADDIEKVVVIGGGLIGLKAAEG
LHLIDKKITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGDGAAITGVTLKSGDFIPCDTVVVAIGVR
PAAGFLKGSGVEVNRGIVVDDLMETNVKGVFAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVTMAGGSKGYQGGIAM
NSIELFKVSTISMGITNPVEPKEYEILTYQDLENYQYRKIVLKDGILAGAVLVGAVDRAGIFAGLIRDRIEVSSFKDHLL
TPDFGFVNLPREIRSTLFAPVGKVTGAYEQARAVGH
>Mature_436_residues
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHVAYGRPLISYLLGGLITEKRMAYLPEDFYEKNRVNLLLNAEVVG
VDTDNRKVTIAGGDVIVYDKLLVATGGDPFVPPIEGMAGKDRIFTFTTWDDAAKLKGIADDIEKVVVIGGGLIGLKAAEG
LHLIDKKITIVELADRILSAAFDRPAGRVVAKKMKANGIDVITEDTVVRIEGDGAAITGVTLKSGDFIPCDTVVVAIGVR
PAAGFLKGSGVEVNRGIVVDDLMETNVKGVFAAGDVAEAKDFFSGVKNPMPIWPDAYIQGDIAGVTMAGGSKGYQGGIAM
NSIELFKVSTISMGITNPVEPKEYEILTYQDLENYQYRKIVLKDGILAGAVLVGAVDRAGIFAGLIRDRIEVSSFKDHLL
TPDFGFVNLPREIRSTLFAPVGKVTGAYEQARAVGH

Specific function: Required for nitrite assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=309, Percent_Identity=30.7443365695793, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI226437568, Length=309, Percent_Identity=30.7443365695793, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI65787454, Length=309, Percent_Identity=30.7443365695793, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI51036596, Length=185, Percent_Identity=31.8918918918919, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4757732, Length=354, Percent_Identity=24.8587570621469, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI22202629, Length=354, Percent_Identity=24.8587570621469, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788892, Length=260, Percent_Identity=27.6923076923077, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=26.4492753623188, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789765, Length=404, Percent_Identity=25.7425742574257, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081763, Length=206, Percent_Identity=32.0388349514563, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=273, Percent_Identity=31.1355311355311, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=409, Percent_Identity=28.8508557457213, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=347, Percent_Identity=27.0893371757925, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639257, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281359715, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281359713, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639250, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18543267, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639252, Length=314, Percent_Identity=28.343949044586, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585130, Length=313, Percent_Identity=24.2811501597444, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI28573993, Length=351, Percent_Identity=26.4957264957265, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24581020, Length=351, Percent_Identity=26.4957264957265, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24585384, Length=209, Percent_Identity=33.0143540669856, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 46598; Mature: 46598

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHVAYGRPLISYLLGGLITEKRMAYL
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHC
PEDFYEKNRVNLLLNAEVVGVDTDNRKVTIAGGDVIVYDKLLVATGGDPFVPPIEGMAGK
CHHHHCCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCC
DRIFTFTTWDDAAKLKGIADDIEKVVVIGGGLIGLKAAEGLHLIDKKITIVELADRILSA
CEEEEEEECCCHHHHCCHHHHHEEEEEECCCEEEEECCCCEEECCCCCHHHHHHHHHHHH
AFDRPAGRVVAKKMKANGIDVITEDTVVRIEGDGAAITGVTLKSGDFIPCDTVVVAIGVR
HHCCCCHHHHHHHHCCCCCEEEECCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEEECC
PAAGFLKGSGVEVNRGIVVDDLMETNVKGVFAAGDVAEAKDFFSGVKNPMPIWPDAYIQG
CCCCCCCCCCEEEECCEEEEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEC
DIAGVTMAGGSKGYQGGIAMNSIELFKVSTISMGITNPVEPKEYEILTYQDLENYQYRKI
CEEEEEEECCCCCCCCCEEECCEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEE
VLKDGILAGAVLVGAVDRAGIFAGLIRDRIEVSSFKDHLLTPDFGFVNLPREIRSTLFAP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHH
VGKVTGAYEQARAVGH
HHHHCCHHHHHHHCCC
>Mature Secondary Structure
MNYVIIGNSVAAVGAIRGIRSIDQQGNITVISRERHVAYGRPLISYLLGGLITEKRMAYL
CCEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHC
PEDFYEKNRVNLLLNAEVVGVDTDNRKVTIAGGDVIVYDKLLVATGGDPFVPPIEGMAGK
CHHHHCCCCEEEEEEEEEEEECCCCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCC
DRIFTFTTWDDAAKLKGIADDIEKVVVIGGGLIGLKAAEGLHLIDKKITIVELADRILSA
CEEEEEEECCCHHHHCCHHHHHEEEEEECCCEEEEECCCCEEECCCCCHHHHHHHHHHHH
AFDRPAGRVVAKKMKANGIDVITEDTVVRIEGDGAAITGVTLKSGDFIPCDTVVVAIGVR
HHCCCCHHHHHHHHCCCCCEEEECCEEEEEECCCEEEEEEEECCCCCCCCCCEEEEEECC
PAAGFLKGSGVEVNRGIVVDDLMETNVKGVFAAGDVAEAKDFFSGVKNPMPIWPDAYIQG
CCCCCCCCCCEEEECCEEEEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEC
DIAGVTMAGGSKGYQGGIAMNSIELFKVSTISMGITNPVEPKEYEILTYQDLENYQYRKI
CEEEEEEECCCCCCCCCEEECCEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEEE
VLKDGILAGAVLVGAVDRAGIFAGLIRDRIEVSSFKDHLLTPDFGFVNLPREIRSTLFAP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHH
VGKVTGAYEQARAVGH
HHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]