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The map label for this gene is gltB [C]

Identifier: 78222965

GI number: 78222965

Start: 1971016

End: 1972545

Strand: Reverse

Name: gltB [C]

Synonym: Gmet_1756

Alternate gene names: 78222965

Gene position: 1972545-1971016 (Counterclockwise)

Preceding gene: 78222966

Following gene: 78222964

Centisome position: 49.35

GC content: 63.27

Gene sequence:

>1530_bases
GTGCTCTACAAATCTGTAACGGAATCGTTCAACGAATTCATCATCGACCGCAGCGACGCCACATGCATCCGCTGCAAGGT
CTGCGTCCGCCAGTGCGCCTACGAGGTCCACGCCTATGAAGCCAAGGCCGACGCCCTGACCGAGGACAACACCCTCTGCA
TCGGCTGCCGCCGTTGCTCGGCCCTCTGCCCCACCGGCGCCATCACCATCCGGAGCAACGAAGAGGTGTTCAAGCGGAAC
GAATCGTGGTCCAACGCCCACATCCGCAACCTTTACGCCCAGGCCGACACGGGGGGGATTCTCCTGGCCGCCATGGGAAA
CCCGGCCAAGTATCCCATCTACTGGGATCACATGCTGCTGGACGCCTCCCAGGTGACCAACCCTTCCATCGACCCCCTTC
GGGAGCCCATGGAGCTGCGGACCTACCTGGGTAAGAAGCCCCATTCCATCGAGGTGGTCCGTGACGAGCAGACCGGCAAG
CCCAAGCTCGCCACAAAGCTCACCCCCCAGATCAAGATGGAGTACCCCTTCATCTTCTCCGCCATGAGCTACGGGGCTCT
TAACCTCAACGCCCATAAGGCCATGGCCATGGCAGCCAAGGAACTGGGCACCCTCTACAACACCGGCGAGGGTGGACTCC
ACAGGGATCTCTACCAGTACGGCAGCAACGTCATGGTACAGGTGGCATCGGGACGCTTCGGTGTGAGCGAAGCCTATCTG
AACGCCGGCGTTGCCATCGAGATCAAGGTGGGCCAGGGGGCAAAGCCGGGGATCGGCGGCCACCTCCCCGGCGAGAAGGT
TAACGATCAGATTTCCGAAACCCGGATGATCCCGGCCGGGGCTGACGCCATATCGCCGGCGCCCCACCACGACATTTACT
CCATCGAAGATTTGCGCCAGTTGATCTTCGCCCTCAAGGAAGCCACCAACTACACGAAGCCGGTGTCGGTGAAGATCGCT
GCGGTCCACCACGTGGCGGCCATCGCCTCGGGCGTGGCCCGGGCCGGAGCCGACATCATCACCATCGACGGTTTCCGGGG
AGGGACCGGCGCGGCGCCCCAAGTGATTCGCGACAACGTCGGCATCCCCATGGAGCTGGCCCTGGCCGCCGTTGACGCAC
GGCTCCGGGACGAGGGGATCAGGAACCAGGTATCCATCGTGGTGGGGGGTGGGGTTCGCTCATCAGGGGACGCCATCAAG
GCCATTGCCCTTGGGGCCGACGCCATCAACATGGGGACCTCGACGCTTCTGGCCCTGGGATGCACCCTCTGTCAGCGCTG
CTACACCGGCAAGTGCCCCTGGGGGATCACCACCAACAACCCGTACCTCGCCAAGCGCCTCAACCCCGAACTTGGTGCCG
AGCGGTTGGTGAACCTGGTCCACGCCTGGGGCCACGAGATGAAGGAAATCCTGGGCGGCATGGGGCTCAACGCCCTGGAG
TCGCTGCGGGGCAACCGTTACAAGCTCCGCGCCCTTGGCCTCACCGAGAAAGACATGAACATCCTGGGCGTCATGCCCGC
TGGAGAATAA

Upstream 100 bases:

>100_bases
ATCAGAAACCACAAAACTGGTACGCCTCTTGTATAACCTGTAAGCCACGAGGCACGAGAGTACCCACACACTCGCGCACC
CGCTTTCAGGAGGTTCCACG

Downstream 100 bases:

>100_bases
GGTATGAAGCGCATCTACATGATCGAAGACGTCTGCATCGGCTGCCACCTCTGTGAGGTGGCGTGCATCACCGAGCACTC
CCAGTCCAAAAACCCGGTCA

Product: glutamate synthase (NADPH) GltB2 subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MLYKSVTESFNEFIIDRSDATCIRCKVCVRQCAYEVHAYEAKADALTEDNTLCIGCRRCSALCPTGAITIRSNEEVFKRN
ESWSNAHIRNLYAQADTGGILLAAMGNPAKYPIYWDHMLLDASQVTNPSIDPLREPMELRTYLGKKPHSIEVVRDEQTGK
PKLATKLTPQIKMEYPFIFSAMSYGALNLNAHKAMAMAAKELGTLYNTGEGGLHRDLYQYGSNVMVQVASGRFGVSEAYL
NAGVAIEIKVGQGAKPGIGGHLPGEKVNDQISETRMIPAGADAISPAPHHDIYSIEDLRQLIFALKEATNYTKPVSVKIA
AVHHVAAIASGVARAGADIITIDGFRGGTGAAPQVIRDNVGIPMELALAAVDARLRDEGIRNQVSIVVGGGVRSSGDAIK
AIALGADAINMGTSTLLALGCTLCQRCYTGKCPWGITTNNPYLAKRLNPELGAERLVNLVHAWGHEMKEILGGMGLNALE
SLRGNRYKLRALGLTEKDMNILGVMPAGE

Sequences:

>Translated_509_residues
MLYKSVTESFNEFIIDRSDATCIRCKVCVRQCAYEVHAYEAKADALTEDNTLCIGCRRCSALCPTGAITIRSNEEVFKRN
ESWSNAHIRNLYAQADTGGILLAAMGNPAKYPIYWDHMLLDASQVTNPSIDPLREPMELRTYLGKKPHSIEVVRDEQTGK
PKLATKLTPQIKMEYPFIFSAMSYGALNLNAHKAMAMAAKELGTLYNTGEGGLHRDLYQYGSNVMVQVASGRFGVSEAYL
NAGVAIEIKVGQGAKPGIGGHLPGEKVNDQISETRMIPAGADAISPAPHHDIYSIEDLRQLIFALKEATNYTKPVSVKIA
AVHHVAAIASGVARAGADIITIDGFRGGTGAAPQVIRDNVGIPMELALAAVDARLRDEGIRNQVSIVVGGGVRSSGDAIK
AIALGADAINMGTSTLLALGCTLCQRCYTGKCPWGITTNNPYLAKRLNPELGAERLVNLVHAWGHEMKEILGGMGLNALE
SLRGNRYKLRALGLTEKDMNILGVMPAGE
>Mature_509_residues
MLYKSVTESFNEFIIDRSDATCIRCKVCVRQCAYEVHAYEAKADALTEDNTLCIGCRRCSALCPTGAITIRSNEEVFKRN
ESWSNAHIRNLYAQADTGGILLAAMGNPAKYPIYWDHMLLDASQVTNPSIDPLREPMELRTYLGKKPHSIEVVRDEQTGK
PKLATKLTPQIKMEYPFIFSAMSYGALNLNAHKAMAMAAKELGTLYNTGEGGLHRDLYQYGSNVMVQVASGRFGVSEAYL
NAGVAIEIKVGQGAKPGIGGHLPGEKVNDQISETRMIPAGADAISPAPHHDIYSIEDLRQLIFALKEATNYTKPVSVKIA
AVHHVAAIASGVARAGADIITIDGFRGGTGAAPQVIRDNVGIPMELALAAVDARLRDEGIRNQVSIVVGGGVRSSGDAIK
AIALGADAINMGTSTLLALGCTLCQRCYTGKCPWGITTNNPYLAKRLNPELGAERLVNLVHAWGHEMKEILGGMGLNALE
SLRGNRYKLRALGLTEKDMNILGVMPAGE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=339, Percent_Identity=41.0029498525074, Blast_Score=209, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17570289, Length=327, Percent_Identity=39.1437308868502, Blast_Score=224, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6320030, Length=331, Percent_Identity=38.368580060423, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI28574881, Length=348, Percent_Identity=37.6436781609195, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24665539, Length=348, Percent_Identity=37.6436781609195, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54868; Mature: 54868

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYKSVTESFNEFIIDRSDATCIRCKVCVRQCAYEVHAYEAKADALTEDNTLCIGCRRCS
CCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHC
ALCPTGAITIRSNEEVFKRNESWSNAHIRNLYAQADTGGILLAAMGNPAKYPIYWDHMLL
CCCCCCEEEECCCHHHHHCCCCCCCCHHHHHEEECCCCCEEEEECCCCCCCCEEEHHHEE
DASQVTNPSIDPLREPMELRTYLGKKPHSIEVVRDEQTGKPKLATKLTPQIKMEYPFIFS
EHHHCCCCCHHHHHCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCEEECCCCHHH
AMSYGALNLNAHKAMAMAAKELGTLYNTGEGGLHRDLYQYGSNVMVQVASGRFGVSEAYL
HHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHH
NAGVAIEIKVGQGAKPGIGGHLPGEKVNDQISETRMIPAGADAISPAPHHDIYSIEDLRQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LIFALKEATNYTKPVSVKIAAVHHVAAIASGVARAGADIITIDGFRGGTGAAPQVIRDNV
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCC
GIPMELALAAVDARLRDEGIRNQVSIVVGGGVRSSGDAIKAIALGADAINMGTSTLLALG
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCHHHCCHHHHHHHH
CTLCQRCYTGKCPWGITTNNPYLAKRLNPELGAERLVNLVHAWGHEMKEILGGMGLNALE
HHHHHHHHCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SLRGNRYKLRALGLTEKDMNILGVMPAGE
HHCCCCEEEEEECCCCCCCCEEEEECCCC
>Mature Secondary Structure
MLYKSVTESFNEFIIDRSDATCIRCKVCVRQCAYEVHAYEAKADALTEDNTLCIGCRRCS
CCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHC
ALCPTGAITIRSNEEVFKRNESWSNAHIRNLYAQADTGGILLAAMGNPAKYPIYWDHMLL
CCCCCCEEEECCCHHHHHCCCCCCCCHHHHHEEECCCCCEEEEECCCCCCCCEEEHHHEE
DASQVTNPSIDPLREPMELRTYLGKKPHSIEVVRDEQTGKPKLATKLTPQIKMEYPFIFS
EHHHCCCCCHHHHHCHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCEEECCCCHHH
AMSYGALNLNAHKAMAMAAKELGTLYNTGEGGLHRDLYQYGSNVMVQVASGRFGVSEAYL
HHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHH
NAGVAIEIKVGQGAKPGIGGHLPGEKVNDQISETRMIPAGADAISPAPHHDIYSIEDLRQ
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
LIFALKEATNYTKPVSVKIAAVHHVAAIASGVARAGADIITIDGFRGGTGAAPQVIRDNV
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHCCC
GIPMELALAAVDARLRDEGIRNQVSIVVGGGVRSSGDAIKAIALGADAINMGTSTLLALG
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEEECCCHHHCCHHHHHHHH
CTLCQRCYTGKCPWGITTNNPYLAKRLNPELGAERLVNLVHAWGHEMKEILGGMGLNALE
HHHHHHHHCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SLRGNRYKLRALGLTEKDMNILGVMPAGE
HHCCCCEEEEEECCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]