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The map label for this gene is nifU [H]

Identifier: 78222211

GI number: 78222211

Start: 1099747

End: 1100607

Strand: Direct

Name: nifU [H]

Synonym: Gmet_0991

Alternate gene names: 78222211

Gene position: 1099747-1100607 (Clockwise)

Preceding gene: 78222210

Following gene: 78222212

Centisome position: 27.51

GC content: 62.49

Gene sequence:

>861_bases
ATGTGGGATTATACTGAAAAGGTTCGGGAACATTTCCTGAACCCGCGCAACGTTGGCGATATTCCCGATGCGGACGCAGT
GGGGGAGGTGGGAAGCCTTGCCTGCGGCGACGCCCTCAAGCTCTATATCAAACTCGACGACGCCAAGGAGCGGATTGTCG
ACGCCAAGTTCCAGACCTTTGGCTGCGCCAGCGCCATCGCGTCGTCATCGGCCCTGACCGAGATCATCAAGGGGAAAACC
CTCGACGAGGCCCTGGCCACCACCAACCAGGAGATTGCCGATTTCCTCGGCGGGCTTCCCGAGGAGAAGATGCACTGTTC
GGTCATGGGGCAGGAGGCCCTTGAGGTGGCCATCGCCAAGTATCGCGGCGGTCCGGTGCCGACCCACCACGACCACGGCC
ACGAGGAGGTGGAGCCGGGAGAGATCGTCTGCAAGTGCTTCGGCCTCACCGACACCTTCCTGAAGAAGGTCATCGCCGCC
AACAAGCTGACCACGGCGGAGCAGGTGACCCACTTCACCAAGGCCGGCGGCGCCTGCGGCGGCTGCATCCCGAAAATCAA
GGAGCTGATCAGCGAGGTGCTCGGGGCCCAGCCCACCGAGGAGCGGAAAAAGCCAGAAAAGCTCACCAACCTCCGCAAGA
TGCAGCTCATCCAGGAGACCCTTGAGAAGGAAATCCGCCCGCTTCTGTGGGCCGACGGCGGCGACCTGGAGTTGGTGGAC
ATCTCGGGGAGCGAGGTTCAGATCGCTTTCCGCAAGGCCTGCGCCGGCTGCGCCTCGTCGGGCAACACCGCCAAGTTCGT
GGAGTTGAAACTGCGGGATCTGGTGGCTGACGACATCGTTGTCCAGGAGGTGGAGGGATGA

Upstream 100 bases:

>100_bases
CCATTCGTTTTTTGACATGCAGAAGAATGGAGTCTTGAATACCTGACAATTTTGCTATAGTATTGCGTAGCGATGAACGT
TACTCCAAGGAGGATTTTCC

Downstream 100 bases:

>100_bases
AAGAGATCTACATGGACAACAACGCCACCACCAAGGTGGACGAGGCTGTCTTCGAGGAGATGCGTCCCTATTTCTGCGAT
CTCTACGGCAACCCCAGCTC

Product: Fe-S cluster assembly protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MWDYTEKVREHFLNPRNVGDIPDADAVGEVGSLACGDALKLYIKLDDAKERIVDAKFQTFGCASAIASSSALTEIIKGKT
LDEALATTNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGGPVPTHHDHGHEEVEPGEIVCKCFGLTDTFLKKVIAA
NKLTTAEQVTHFTKAGGACGGCIPKIKELISEVLGAQPTEERKKPEKLTNLRKMQLIQETLEKEIRPLLWADGGDLELVD
ISGSEVQIAFRKACAGCASSGNTAKFVELKLRDLVADDIVVQEVEG

Sequences:

>Translated_286_residues
MWDYTEKVREHFLNPRNVGDIPDADAVGEVGSLACGDALKLYIKLDDAKERIVDAKFQTFGCASAIASSSALTEIIKGKT
LDEALATTNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGGPVPTHHDHGHEEVEPGEIVCKCFGLTDTFLKKVIAA
NKLTTAEQVTHFTKAGGACGGCIPKIKELISEVLGAQPTEERKKPEKLTNLRKMQLIQETLEKEIRPLLWADGGDLELVD
ISGSEVQIAFRKACAGCASSGNTAKFVELKLRDLVADDIVVQEVEG
>Mature_286_residues
MWDYTEKVREHFLNPRNVGDIPDADAVGEVGSLACGDALKLYIKLDDAKERIVDAKFQTFGCASAIASSSALTEIIKGKT
LDEALATTNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGGPVPTHHDHGHEEVEPGEIVCKCFGLTDTFLKKVIAA
NKLTTAEQVTHFTKAGGACGGCIPKIKELISEVLGAQPTEERKKPEKLTNLRKMQLIQETLEKEIRPLLWADGGDLELVD
ISGSEVQIAFRKACAGCASSGNTAKFVELKLRDLVADDIVVQEVEG

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=122, Percent_Identity=50, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI24307953, Length=123, Percent_Identity=48.780487804878, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788878, Length=123, Percent_Identity=52.0325203252033, Blast_Score=118, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17543474, Length=122, Percent_Identity=50.8196721311475, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=47.5806451612903, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324800, Length=128, Percent_Identity=46.09375, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355597, Length=122, Percent_Identity=51.6393442622951, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 30964; Mature: 30964

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYTEKVREHFLNPRNVGDIPDADAVGEVGSLACGDALKLYIKLDDAKERIVDAKFQTF
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHH
GCASAIASSSALTEIIKGKTLDEALATTNQEIADFLGGLPEEKMHCSVMGQEALEVAIAK
HHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YRGGPVPTHHDHGHEEVEPGEIVCKCFGLTDTFLKKVIAANKLTTAEQVTHFTKAGGACG
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
GCIPKIKELISEVLGAQPTEERKKPEKLTNLRKMQLIQETLEKEIRPLLWADGGDLELVD
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
ISGSEVQIAFRKACAGCASSGNTAKFVELKLRDLVADDIVVQEVEG
CCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MWDYTEKVREHFLNPRNVGDIPDADAVGEVGSLACGDALKLYIKLDDAKERIVDAKFQTF
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHH
GCASAIASSSALTEIIKGKTLDEALATTNQEIADFLGGLPEEKMHCSVMGQEALEVAIAK
HHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YRGGPVPTHHDHGHEEVEPGEIVCKCFGLTDTFLKKVIAANKLTTAEQVTHFTKAGGACG
HCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
GCIPKIKELISEVLGAQPTEERKKPEKLTNLRKMQLIQETLEKEIRPLLWADGGDLELVD
CHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
ISGSEVQIAFRKACAGCASSGNTAKFVELKLRDLVADDIVVQEVEG
CCCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]