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The map label for this gene is nifS1 [H]

Identifier: 78222212

GI number: 78222212

Start: 1100604

End: 1101779

Strand: Direct

Name: nifS1 [H]

Synonym: Gmet_0992

Alternate gene names: 78222212

Gene position: 1100604-1101779 (Clockwise)

Preceding gene: 78222211

Following gene: 78222213

Centisome position: 27.53

GC content: 64.03

Gene sequence:

>1176_bases
ATGAAAGAGATCTACATGGACAACAACGCCACCACCAAGGTGGACGAGGCTGTCTTCGAGGAGATGCGTCCCTATTTCTG
CGATCTCTACGGCAACCCCAGCTCCATGCACTTCTTCGGCGGCCAGGTGCAGAAAAAGGTGGACGAGGCAAGAAACCGCG
TGGCGAACCTTCTGGGGGCTTCCCCCGAGGAGATCGTCTTCACCGCCTGCGGCACCGAGAGCGACAACGCGGCCATCCGC
TCGGCCATCGAGGTCCATCCGGAGCGGCGCCACATCATCACCACCCGCGTGGAGCACCCGGCGGTTCTGACCCTCTGCCG
CAACCTTTCCAAGCGTGGGTACCGGGTCACCGAACTGAACGTGGACGGCGAGGGGAGGCTGGACCTGAACGAACTGCGGG
CAGCCGTTGACGAGGACACGGCCATCGTGTCGGTCATGTGGGCCAACAACGAAACCGGCGTGGTCTTTCCCATCGAGGAG
GTGGGGAAAATCGTCAAGGAGAAGGGGAAGGGCGCCTTCTTCCATACCGACGCCGTCCAGGCAGTGGGAAAGATTCCCAT
CAACATGGCCGCCTCCACCGTGGACATGCTCTCCATTTCGGGGCACAAGCTCCACGCGCCCAAGGGGATCGGGGTCCTCT
ACGTCCGCAAGGGAGTTCCGTTCCGGCCGTTCATGGTCGGGGGGCACCAGGAGCACAACCGTCGGGCCGGCACCGAGAAC
ACCGCCGCCATCATCGCCCTCGGCAAGGCGTGCGAACTGGCCGGTCAGTGGATGGAGGACGAGAACACCCGCGTGAAGGC
CATGCGGGACCGGCTCCAGACAACGCTCCTTGAACTGATACCCCGGTCCCGCATCAACGGCGGCCAGGCGGAGCGGCTTC
CCAACACCCTCTCCATCGCCTTCGAGTTCGTGGAAGGGGAGGCGATCCTGATGCTCCTGTCGGAGAAGGGGATCTGCGCT
TCGTCGGGGAGTGCCTGCACCTCCGGCTCCCTGGAGCCCTCCCACGTGCTTCGCGCCATGGGGGTGCCGTTCACCTGCGC
CCACGGCTCCATCCGCTTCTCCCTGTCTCGCTTCAACACCGACGAGGATATCGAGACCATCATCCGGGAACTGCCGCCGG
TAGTCCGGAGGCTGCGGGAAATGTCCCCCTTCGGGCGGGAGTTTCTCAACGCCTGA

Upstream 100 bases:

>100_bases
CCTGCGCCGGCTGCGCCTCGTCGGGCAACACCGCCAAGTTCGTGGAGTTGAAACTGCGGGATCTGGTGGCTGACGACATC
GTTGTCCAGGAGGTGGAGGG

Downstream 100 bases:

>100_bases
CCATAAACAGGAGATTGTCACAGGAGAAGGGAAGGGGACGCCGCGAGAATGGCGTCCCCTTTTGTTTGCAAGCTCGGCGG
AATCCCCCCTGTGACGGTTG

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKEIYMDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHFFGGQVQKKVDEARNRVANLLGASPEEIVFTACGTESDNAAIR
SAIEVHPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELNVDGEGRLDLNELRAAVDEDTAIVSVMWANNETGVVFPIEE
VGKIVKEKGKGAFFHTDAVQAVGKIPINMAASTVDMLSISGHKLHAPKGIGVLYVRKGVPFRPFMVGGHQEHNRRAGTEN
TAAIIALGKACELAGQWMEDENTRVKAMRDRLQTTLLELIPRSRINGGQAERLPNTLSIAFEFVEGEAILMLLSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFNTDEDIETIIRELPPVVRRLREMSPFGREFLNA

Sequences:

>Translated_391_residues
MKEIYMDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHFFGGQVQKKVDEARNRVANLLGASPEEIVFTACGTESDNAAIR
SAIEVHPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELNVDGEGRLDLNELRAAVDEDTAIVSVMWANNETGVVFPIEE
VGKIVKEKGKGAFFHTDAVQAVGKIPINMAASTVDMLSISGHKLHAPKGIGVLYVRKGVPFRPFMVGGHQEHNRRAGTEN
TAAIIALGKACELAGQWMEDENTRVKAMRDRLQTTLLELIPRSRINGGQAERLPNTLSIAFEFVEGEAILMLLSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFNTDEDIETIIRELPPVVRRLREMSPFGREFLNA
>Mature_391_residues
MKEIYMDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHFFGGQVQKKVDEARNRVANLLGASPEEIVFTACGTESDNAAIR
SAIEVHPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELNVDGEGRLDLNELRAAVDEDTAIVSVMWANNETGVVFPIEE
VGKIVKEKGKGAFFHTDAVQAVGKIPINMAASTVDMLSISGHKLHAPKGIGVLYVRKGVPFRPFMVGGHQEHNRRAGTEN
TAAIIALGKACELAGQWMEDENTRVKAMRDRLQTTLLELIPRSRINGGQAERLPNTLSIAFEFVEGEAILMLLSEKGICA
SSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFNTDEDIETIIRELPPVVRRLREMSPFGREFLNA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=388, Percent_Identity=44.0721649484536, Blast_Score=335, Evalue=3e-92,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=32.6139088729017, Blast_Score=208, Evalue=7e-54,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=44.702842377261, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1789175, Length=387, Percent_Identity=25.8397932816537, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787970, Length=400, Percent_Identity=26.25, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=41.4507772020725, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=33.9622641509434, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=41.8604651162791, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI20129463, Length=387, Percent_Identity=42.3772609819121, Blast_Score=313, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43139; Mature: 43139

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIYMDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHFFGGQVQKKVDEARNRVANLLGA
CCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCC
SPEEIVFTACGTESDNAAIRSAIEVHPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELN
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDGEGRLDLNELRAAVDEDTAIVSVMWANNETGVVFPIEEVGKIVKEKGKGAFFHTDAVQ
CCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEHHHHH
AVGKIPINMAASTVDMLSISGHKLHAPKGIGVLYVRKGVPFRPFMVGGHQEHNRRAGTEN
HHHCCCCHHHHHHHHHEECCCCEEECCCCCEEEEEECCCCCCCEEECCCHHHCCCCCCCC
TAAIIALGKACELAGQWMEDENTRVKAMRDRLQTTLLELIPRSRINGGQAERLPNTLSIA
CEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEE
FEFVEGEAILMLLSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFNT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCC
DEDIETIIRELPPVVRRLREMSPFGREFLNA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKEIYMDNNATTKVDEAVFEEMRPYFCDLYGNPSSMHFFGGQVQKKVDEARNRVANLLGA
CCEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCC
SPEEIVFTACGTESDNAAIRSAIEVHPERRHIITTRVEHPAVLTLCRNLSKRGYRVTELN
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEE
VDGEGRLDLNELRAAVDEDTAIVSVMWANNETGVVFPIEEVGKIVKEKGKGAFFHTDAVQ
CCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHCCCCCEEEHHHHH
AVGKIPINMAASTVDMLSISGHKLHAPKGIGVLYVRKGVPFRPFMVGGHQEHNRRAGTEN
HHHCCCCHHHHHHHHHEECCCCEEECCCCCEEEEEECCCCCCCEEECCCHHHCCCCCCCC
TAAIIALGKACELAGQWMEDENTRVKAMRDRLQTTLLELIPRSRINGGQAERLPNTLSIA
CEEEEEECHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEE
FEFVEGEAILMLLSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFNT
EEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEEECCCC
DEDIETIIRELPPVVRRLREMSPFGREFLNA
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]