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The map label for this gene is queA

Identifier: 78222071

GI number: 78222071

Start: 937712

End: 938743

Strand: Direct

Name: queA

Synonym: Gmet_0851

Alternate gene names: 78222071

Gene position: 937712-938743 (Clockwise)

Preceding gene: 78222070

Following gene: 78222072

Centisome position: 23.46

GC content: 60.76

Gene sequence:

>1032_bases
ATGCGCGTTGACGATTTCGACTATGAACTGCCTCCGGAGTTGATAGCCCAGGAACCCTTGGACCGACGTGACGCCACCCG
ACTCATGACCGTCGACCGGTTGAAGGGAGAGGTCGGCGAGGTCTCTTTCCGCGAAATAACCCGGCTTTTCCGGCCGGGAG
ACCTCCTGGTGGTGAACGACACCCGTGTCATTCCGGCGCGTTTTTTCGGCGTCAAGGAGAGTGGTGGCCGTGTGGAGGTT
TTCCTGGAAAGGAGGCTTGCCGCCGTGGGAGAACGGTGGCAGTGTCTGCTCCGTTCCTCCAAGCCGTCGCGACCCGGTAC
CCGGATAGTGCTCGCGGAAGGTGTGTCCGCGGAGGTCATCGGGCGCAGTGACGGGGATACGTGGTGTGTTTCGTTTACCC
CCGTGACAGGGTTCGATGAATGGCTGGAACGCAACGGCGCAATGCCTCTGCCTCCTTACATAAGGAGGAGCGCGGCTGAC
GCAGACCGGGAGCGGTATCAGACGGTTTTTTCCCGCCAGCGTGGCGCGGTCGCCGCACCGACGGCAGGCCTCCATTTTAC
TCCCGAACTGCTGGATGAGCTACGGGAGCGAGGCGTAGAGATTGCCACCCTTACCCTCCATGTGGGGCTCGGTACGTTTA
TGCCAATCAGGGTTGAACGGGTGGAAGAGCACCGGATGCACCGTGAGCGGTATATCATCCCCTCTGCGACCGCCGACGCG
GTGAATGCCCGGAAGGGGGGCAAAGGGAGGGTAATCGCTCTGGGAACCACCACGTGCCGCACCCTGGAGCATGCCGCCCG
TGACGATGGCCGCGTGGCAGCCGGCGCGGGAGAAACAGGCATCTTTATCTATCCGGGCTACCACTTCAAAACGGTGGATG
CCCTCATTACCAATTTTCACCTCCCCAAATCGACCCTGCTCATGCTCGTTTCGGCCTTTGCCGGGAAGGAGCTTCTCTTC
AGGGCATACCATGAGGCGGTGACTCGGCGTTTCCGGTTTTTCAGCTACGGCGACGCGATGTTTGTACATTAG

Upstream 100 bases:

>100_bases
GCGAAACAACGGGCATCCGATGGTTTCACCTACCGGGAGATTCTTGCCTATTACTATCCCGGTGCGCTCCTGAGCAAGCT
CCCGGTGGGGCAGGGTGTCG

Downstream 100 bases:

>100_bases
GCCGCTTGCCGTTGCCGGCTGACCGCGAGATAATCAGGACGGATTTTGATCCATTTCAAACTTATAAAGAAAGACGGCGC
AGCCCGTCTCGGCTCTTTGA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MRVDDFDYELPPELIAQEPLDRRDATRLMTVDRLKGEVGEVSFREITRLFRPGDLLVVNDTRVIPARFFGVKESGGRVEV
FLERRLAAVGERWQCLLRSSKPSRPGTRIVLAEGVSAEVIGRSDGDTWCVSFTPVTGFDEWLERNGAMPLPPYIRRSAAD
ADRERYQTVFSRQRGAVAAPTAGLHFTPELLDELRERGVEIATLTLHVGLGTFMPIRVERVEEHRMHRERYIIPSATADA
VNARKGGKGRVIALGTTTCRTLEHAARDDGRVAAGAGETGIFIYPGYHFKTVDALITNFHLPKSTLLMLVSAFAGKELLF
RAYHEAVTRRFRFFSYGDAMFVH

Sequences:

>Translated_343_residues
MRVDDFDYELPPELIAQEPLDRRDATRLMTVDRLKGEVGEVSFREITRLFRPGDLLVVNDTRVIPARFFGVKESGGRVEV
FLERRLAAVGERWQCLLRSSKPSRPGTRIVLAEGVSAEVIGRSDGDTWCVSFTPVTGFDEWLERNGAMPLPPYIRRSAAD
ADRERYQTVFSRQRGAVAAPTAGLHFTPELLDELRERGVEIATLTLHVGLGTFMPIRVERVEEHRMHRERYIIPSATADA
VNARKGGKGRVIALGTTTCRTLEHAARDDGRVAAGAGETGIFIYPGYHFKTVDALITNFHLPKSTLLMLVSAFAGKELLF
RAYHEAVTRRFRFFSYGDAMFVH
>Mature_343_residues
MRVDDFDYELPPELIAQEPLDRRDATRLMTVDRLKGEVGEVSFREITRLFRPGDLLVVNDTRVIPARFFGVKESGGRVEV
FLERRLAAVGERWQCLLRSSKPSRPGTRIVLAEGVSAEVIGRSDGDTWCVSFTPVTGFDEWLERNGAMPLPPYIRRSAAD
ADRERYQTVFSRQRGAVAAPTAGLHFTPELLDELRERGVEIATLTLHVGLGTFMPIRVERVEEHRMHRERYIIPSATADA
VNARKGGKGRVIALGTTTCRTLEHAARDDGRVAAGAGETGIFIYPGYHFKTVDALITNFHLPKSTLLMLVSAFAGKELLF
RAYHEAVTRRFRFFSYGDAMFVH

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=50, Blast_Score=325, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_GEOMG (Q39XD1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383818.1
- ProteinModelPortal:   Q39XD1
- SMR:   Q39XD1
- STRING:   Q39XD1
- GeneID:   3738728
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0851
- NMPDR:   fig|269799.3.peg.915
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q39XD1
- ProtClustDB:   PRK00147
- BioCyc:   GMET269799:GMET_0851-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38552; Mature: 38552

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDDFDYELPPELIAQEPLDRRDATRLMTVDRLKGEVGEVSFREITRLFRPGDLLVVND
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECC
TRVIPARFFGVKESGGRVEVFLERRLAAVGERWQCLLRSSKPSRPGTRIVLAEGVSAEVI
CEEEEHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEE
GRSDGDTWCVSFTPVTGFDEWLERNGAMPLPPYIRRSAADADRERYQTVFSRQRGAVAAP
ECCCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEECC
TAGLHFTPELLDELRERGVEIATLTLHVGLGTFMPIRVERVEEHRMHRERYIIPSATADA
CCCCEECHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHH
VNARKGGKGRVIALGTTTCRTLEHAARDDGRVAAGAGETGIFIYPGYHFKTVDALITNFH
HHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHCC
LPKSTLLMLVSAFAGKELLFRAYHEAVTRRFRFFSYGDAMFVH
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEC
>Mature Secondary Structure
MRVDDFDYELPPELIAQEPLDRRDATRLMTVDRLKGEVGEVSFREITRLFRPGDLLVVND
CCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECC
TRVIPARFFGVKESGGRVEVFLERRLAAVGERWQCLLRSSKPSRPGTRIVLAEGVSAEVI
CEEEEHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEE
GRSDGDTWCVSFTPVTGFDEWLERNGAMPLPPYIRRSAADADRERYQTVFSRQRGAVAAP
ECCCCCEEEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEECC
TAGLHFTPELLDELRERGVEIATLTLHVGLGTFMPIRVERVEEHRMHRERYIIPSATADA
CCCCEECHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCHH
VNARKGGKGRVIALGTTTCRTLEHAARDDGRVAAGAGETGIFIYPGYHFKTVDALITNFH
HHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHCC
LPKSTLLMLVSAFAGKELLFRAYHEAVTRRFRFFSYGDAMFVH
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA