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The map label for this gene is tgt [H]

Identifier: 78222072

GI number: 78222072

Start: 938789

End: 939901

Strand: Direct

Name: tgt [H]

Synonym: Gmet_0852

Alternate gene names: 78222072

Gene position: 938789-939901 (Clockwise)

Preceding gene: 78222071

Following gene: 78222073

Centisome position: 23.48

GC content: 61.01

Gene sequence:

>1113_bases
TTGATCCATTTCAAACTTATAAAGAAAGACGGCGCAGCCCGTCTCGGCTCTTTGACAACCCCCCGCGGCACCATCGAGAC
GCCGATTTTCATGCCGGTCGGAACCCAGGCCACAGTGAAGGCCATGACCCCCGAGGAGCTGAAGACCATCGGTGCCCGGA
TCATCCTGGCCAACACGTACCATCTTTATATTCGCCCCGGCCATGAATTGGTGAGGCGCATGGGCGGGCTTCACCGTTTC
ATGAACTGGGATCGGCCGATCCTTACCGACAGCGGCGGGTTTCAGGTCTTCAGCCTGAACGAACTGCGCAAAATCACCGA
GGAGGGGGTCAAGTTCCGGTCCCATCTGGACGGTTCCTATCATTTTATCTCTCCCGAGGACGCCATTGCCATTCAGGGGG
CTCTGGGGAGCGACATCGCCATGTGTTTCGACGAGTGTACTCCCTACCCGGCCACCCACGACTACGCCCGCCGCTCCATG
GAGATGACCACCCGGTGGGCCAGGCGCTGCAAGGATGCGAAGACGCGGGAGGATCAGGCTCTCTTCGGGATCGTTCAGGG
GGGGATGTACCGGGACCTGCGGGAGCGAAGCGCCGCGGAACTGCGGGAGATCGGCTTTGACGGTTACGCCATGGGAGGGC
TCTCCGTTGGCGAGGAAAAGGAAGTCATGCACGAGGTGCTGGAGTACGCCGCGCCGATGCTTCCCGAAGACCGGCCCCGC
TATGTGATGGGAATCGGGGCGCCGGAGGATCTCATCGAGGCGGTCTGGCACGGCTTCGACATGTTCGATTGCGTGATGCC
CACGCGAAACGCCCGCAACGGGATGCTCTTCACCTCCTTTGGTAGGGTCAACATCAAGGGGGCGGCCTATGCCGAGGACG
CCGGCCCTCTGGATTCCGAATGCGACTGTTACGTCTGTCGCACCTACAGCAGGGCCTATCTGAGGCATCTGTACCGCTCG
GGGGAGATTCTCGCGTCGCGGCTCAACACGTACCATAACCTGTACTATTATCTGTCCCTCATGGCAAAGGCGCGGGCCGC
CATTGCCGAGGGACGGTTCGCCGCGTTCCGGCAGGAGTTTTACGCCAAGAGGGAGTTGCAGGGGCAATCCTAG

Upstream 100 bases:

>100_bases
GGTGACTCGGCGTTTCCGGTTTTTCAGCTACGGCGACGCGATGTTTGTACATTAGGCCGCTTGCCGTTGCCGGCTGACCG
CGAGATAATCAGGACGGATT

Downstream 100 bases:

>100_bases
AGATTGCTCCATAAAAAAAGAGGGGGCTTCGGGCTTCCTCTCTATAAATCGTCCGGAGGTTACACAATGTTCGGAGTTGC
TTTTGCAATGGCTGCACAAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MIHFKLIKKDGAARLGSLTTPRGTIETPIFMPVGTQATVKAMTPEELKTIGARIILANTYHLYIRPGHELVRRMGGLHRF
MNWDRPILTDSGGFQVFSLNELRKITEEGVKFRSHLDGSYHFISPEDAIAIQGALGSDIAMCFDECTPYPATHDYARRSM
EMTTRWARRCKDAKTREDQALFGIVQGGMYRDLRERSAAELREIGFDGYAMGGLSVGEEKEVMHEVLEYAAPMLPEDRPR
YVMGIGAPEDLIEAVWHGFDMFDCVMPTRNARNGMLFTSFGRVNIKGAAYAEDAGPLDSECDCYVCRTYSRAYLRHLYRS
GEILASRLNTYHNLYYYLSLMAKARAAIAEGRFAAFRQEFYAKRELQGQS

Sequences:

>Translated_370_residues
MIHFKLIKKDGAARLGSLTTPRGTIETPIFMPVGTQATVKAMTPEELKTIGARIILANTYHLYIRPGHELVRRMGGLHRF
MNWDRPILTDSGGFQVFSLNELRKITEEGVKFRSHLDGSYHFISPEDAIAIQGALGSDIAMCFDECTPYPATHDYARRSM
EMTTRWARRCKDAKTREDQALFGIVQGGMYRDLRERSAAELREIGFDGYAMGGLSVGEEKEVMHEVLEYAAPMLPEDRPR
YVMGIGAPEDLIEAVWHGFDMFDCVMPTRNARNGMLFTSFGRVNIKGAAYAEDAGPLDSECDCYVCRTYSRAYLRHLYRS
GEILASRLNTYHNLYYYLSLMAKARAAIAEGRFAAFRQEFYAKRELQGQS
>Mature_370_residues
MIHFKLIKKDGAARLGSLTTPRGTIETPIFMPVGTQATVKAMTPEELKTIGARIILANTYHLYIRPGHELVRRMGGLHRF
MNWDRPILTDSGGFQVFSLNELRKITEEGVKFRSHLDGSYHFISPEDAIAIQGALGSDIAMCFDECTPYPATHDYARRSM
EMTTRWARRCKDAKTREDQALFGIVQGGMYRDLRERSAAELREIGFDGYAMGGLSVGEEKEVMHEVLEYAAPMLPEDRPR
YVMGIGAPEDLIEAVWHGFDMFDCVMPTRNARNGMLFTSFGRVNIKGAAYAEDAGPLDSECDCYVCRTYSRAYLRHLYRS
GEILASRLNTYHNLYYYLSLMAKARAAIAEGRFAAFRQEFYAKRELQGQS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=46.1318051575931, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=58.9041095890411, Blast_Score=453, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17544680, Length=358, Percent_Identity=44.1340782122905, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=30, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920510, Length=352, Percent_Identity=45.1704545454545, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI21355311, Length=248, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41931; Mature: 41931

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHFKLIKKDGAARLGSLTTPRGTIETPIFMPVGTQATVKAMTPEELKTIGARIILANTY
CEEEEEECCCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCEEEEEEEE
HLYIRPGHELVRRMGGLHRFMNWDRPILTDSGGFQVFSLNELRKITEEGVKFRSHLDGSY
EEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
HFISPEDAIAIQGALGSDIAMCFDECTPYPATHDYARRSMEMTTRWARRCKDAKTREDQA
EEECCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LFGIVQGGMYRDLRERSAAELREIGFDGYAMGGLSVGEEKEVMHEVLEYAAPMLPEDRPR
HHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
YVMGIGAPEDLIEAVWHGFDMFDCVMPTRNARNGMLFTSFGRVNIKGAAYAEDAGPLDSE
EEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCEECCCCCCCCCC
CDCYVCRTYSRAYLRHLYRSGEILASRLNTYHNLYYYLSLMAKARAAIAEGRFAAFRQEF
CCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKRELQGQS
HHHHHCCCCC
>Mature Secondary Structure
MIHFKLIKKDGAARLGSLTTPRGTIETPIFMPVGTQATVKAMTPEELKTIGARIILANTY
CEEEEEECCCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCEEEEEEEE
HLYIRPGHELVRRMGGLHRFMNWDRPILTDSGGFQVFSLNELRKITEEGVKFRSHLDGSY
EEEEECCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
HFISPEDAIAIQGALGSDIAMCFDECTPYPATHDYARRSMEMTTRWARRCKDAKTREDQA
EEECCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LFGIVQGGMYRDLRERSAAELREIGFDGYAMGGLSVGEEKEVMHEVLEYAAPMLPEDRPR
HHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCC
YVMGIGAPEDLIEAVWHGFDMFDCVMPTRNARNGMLFTSFGRVNIKGAAYAEDAGPLDSE
EEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCEECCCCCCCCCC
CDCYVCRTYSRAYLRHLYRSGEILASRLNTYHNLYYYLSLMAKARAAIAEGRFAAFRQEF
CCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKRELQGQS
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA