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The map label for this gene is ppsA [H]

Identifier: 78221990

GI number: 78221990

Start: 845540

End: 847942

Strand: Reverse

Name: ppsA [H]

Synonym: Gmet_0770

Alternate gene names: 78221990

Gene position: 847942-845540 (Counterclockwise)

Preceding gene: 78221991

Following gene: 78221989

Centisome position: 21.21

GC content: 66.21

Gene sequence:

>2403_bases
ATGACCTGGATCCGCTGGTTCTCCGCAATCACCATCGACGACGTCCCCCTCGTGGGAGGTAAAAACGCCTCCCTCGGGGA
GATGTACCGGGAACTCCGCCCCCTGGGGGTGAAGATACCCAACGGCTTCGCCATAACCGCGGAGGCCTACTGGTACCTGG
TCCGCTCTGCCGGGGTGCTGGACGAGATGCGGCGGACCCTGGCGAAACTCCACAAGGAGGACGTGGACGACCTGGCCTTG
CGAGGCCACCGGGTGCGGGAGCTTATCTGCGGCGCGCCGCTCCCGGCGGACCTGGCGGCGGAAATCACGGCCGCCTACCG
CCGGCTCTGCATTGAGTACGGCGAGAACTGCGATGTGGCGGTCCGCAGCTCCGCCACCGCCGAGGACCTCCCCACCGCCT
CCTTCGCCGGTCAGCAGGAAACCTACCTGAATATCCGGGGCGAGGTGCAGCTCCTGGACGCCTGCCGCCGCTGCTTTGCA
TCGCTCTTCACCGACCGGGCCATCTCCTACCGCATCGACCAGGGGTTCGACCACTTCAAGGTGGGGCTCTCCATCGGGGT
CATGAAGATGGTCCGGTCGGACCGGGCCTCCAGCGGCGTCATCTTCACCCTCGACACCGAGACCGGCTTCCGGGACACGG
TGCTGGTCACCGGCGCCTGGGGCCTGGGGGAGAACGTGGTCCAGGGGGCCGTGAACCCCGACGAGTTCACCGTCTTCAAG
CCGCCCCTCCGCCAGGGATTCCGCCCCATCGTCCGGCGCAAGCTCGGTGAGAAGAAGATCAAGATGATCTACGGCACTGG
CACCTCCAAACACCTGACGAAGAACGTGGAGGTGCCCAGGGAGGAGCGGCGCCACTACTGCCTGGCCGACGCAGAGGTGC
TGGAGCTGGCCCGCCAGGCCCTTCTCATCGAAGAGCACTACTCGAAGCGGGCCGGCCACGACGTCCCCATGGACATCGAA
TGGGCCAAGGACGGGGTTTCGGGGGAGCTCTTCATCGTCCAGGCCCGCCCCGAGACGGTGGAATCCCAGAAGCGGCTCGA
CTTTCTCGAAACATATGTTCTGGAGCGCAAGGGGGAAGTCCTTGTCACCGGCACCAGCGTCGGCGGGAAGATCGCCGTCG
GCCCGGCCCGGGTCATCACCGATGTCCACAAATTGAACGAATTCAGCCCCGGCGAGGTCCTGGTGGCCGACACCACCACT
CCTGACTGGGAGCCGGTCATGAAGACCGCCGCCGCCATCGTCACCAACAAGGGGGGGCGTACCTGCCATGCGGCCATCGT
GAGCCGCGAGCTGGGGATTCCGTCGGTGGTGGGGACCGGCGACGGCACCGGGCGGATCGCCCCGGGCCGGGAGATCACCG
TCTCCTGCGCCGAAGGGGACGTGGGGCGTATTTACGACGGCGCGCTCCCTTTCCGGATTGAGCGGGTGGACCTCTCGGGG
ATGCGCCGCCCCCGGACCAGGATCATGATGAACCTGGGGAACCCGGAGGAGGCCTTCTCCCTCTGCCGGATCCCCAACGA
CGGCGTGGGGCTCGCCCGGATGGAGTTCATCATCACCAACCACATCAAGGTCCACCCCATGGCCCTCGTTCACCCGGAGC
GGGTAGAGGACGAGGCGTCCCGCCGCGAGATCGGGCGCCTCACCGCCGGCTACCCCGATCCGCCCGCCTACTTCGTGGAG
AAGCTGGCCGAGGGGATCGGCACCATCGCCGCTGCCTTCCACCCGAAGCCGGTCATTGTCCGGATGAGCGATTTCAAGTC
CAACGAGTACGCCTCGCTCTTGGGGGGGCGGGCCTTCGAGCCCGCTGAGGAGAACCCCATGATCGGCTTCCGGGGGGCTT
CGCGCTACTACGACGAGCGGTACCGGGAGGGGTTCGCCCTGGAGTGCCGGGCGCTGAAACGGGTGCGGGACGAAATGGGG
CTTGTGAACGTGATCCCCATGATCCCCTTCTGCCGGCGGATCGTGGAGGCGGAGAAGGTCCTGGCGGAGATGGCGGCAAA
CGGCCTCGTGCGGGGAGAGAACGGCCTCGGTGTCTACGTCATGTGCGAGATACCGAACAACGTCATCATGATCGACGAAT
TCTCGCAGCTCTTCGACGGCTTCTCCATCGGCTCCAACGACCTGACCCAGCTCACCCTCGGCATCGACCGGGACTCGGCC
CTGGTGGCCCACGTCTTCGACGAGCGGGACCCGGGGGTCATGCGCCTCATCGCCAGCGTGGTGGAAGGGGCGCGGCGCAA
TCACCGCCACAGCGGCATCTGCGGCCAGGCCCCCAGCGACTACCCGGAGTTCGCCGCCTTCCTGGTTCGGGAGGGGATCG
ACTCCATCTCGTTAAATCCGGACTCGATCCTGAAGATCACCTTGCGGGTGCTGGAGATGGAAAAGGAGATAGAGAAACCA
TGA

Upstream 100 bases:

>100_bases
CCCGGCCTACGGCCACCCTCTCCCCGAGGGAGGGGTTTGGTGTCTCCCCTCGCCCTCGGTGAGAGGGGCTGGGGGCGAGG
GGCAACAGGGAGGTTCCCCC

Downstream 100 bases:

>100_bases
ACCGCGACTGGACCCTCATCGAGACCCTCCTCCCCCAGGACACCAATTCTGCGGGAAACATCTTCGGCGGGGTGCTCATG
AGCCTCATGGACAAGGCGGC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MTWIRWFSAITIDDVPLVGGKNASLGEMYRELRPLGVKIPNGFAITAEAYWYLVRSAGVLDEMRRTLAKLHKEDVDDLAL
RGHRVRELICGAPLPADLAAEITAAYRRLCIEYGENCDVAVRSSATAEDLPTASFAGQQETYLNIRGEVQLLDACRRCFA
SLFTDRAISYRIDQGFDHFKVGLSIGVMKMVRSDRASSGVIFTLDTETGFRDTVLVTGAWGLGENVVQGAVNPDEFTVFK
PPLRQGFRPIVRRKLGEKKIKMIYGTGTSKHLTKNVEVPREERRHYCLADAEVLELARQALLIEEHYSKRAGHDVPMDIE
WAKDGVSGELFIVQARPETVESQKRLDFLETYVLERKGEVLVTGTSVGGKIAVGPARVITDVHKLNEFSPGEVLVADTTT
PDWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTGRIAPGREITVSCAEGDVGRIYDGALPFRIERVDLSG
MRRPRTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKVHPMALVHPERVEDEASRREIGRLTAGYPDPPAYFVE
KLAEGIGTIAAAFHPKPVIVRMSDFKSNEYASLLGGRAFEPAEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMG
LVNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLGVYVMCEIPNNVIMIDEFSQLFDGFSIGSNDLTQLTLGIDRDSA
LVAHVFDERDPGVMRLIASVVEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSILKITLRVLEMEKEIEKP

Sequences:

>Translated_800_residues
MTWIRWFSAITIDDVPLVGGKNASLGEMYRELRPLGVKIPNGFAITAEAYWYLVRSAGVLDEMRRTLAKLHKEDVDDLAL
RGHRVRELICGAPLPADLAAEITAAYRRLCIEYGENCDVAVRSSATAEDLPTASFAGQQETYLNIRGEVQLLDACRRCFA
SLFTDRAISYRIDQGFDHFKVGLSIGVMKMVRSDRASSGVIFTLDTETGFRDTVLVTGAWGLGENVVQGAVNPDEFTVFK
PPLRQGFRPIVRRKLGEKKIKMIYGTGTSKHLTKNVEVPREERRHYCLADAEVLELARQALLIEEHYSKRAGHDVPMDIE
WAKDGVSGELFIVQARPETVESQKRLDFLETYVLERKGEVLVTGTSVGGKIAVGPARVITDVHKLNEFSPGEVLVADTTT
PDWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTGRIAPGREITVSCAEGDVGRIYDGALPFRIERVDLSG
MRRPRTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKVHPMALVHPERVEDEASRREIGRLTAGYPDPPAYFVE
KLAEGIGTIAAAFHPKPVIVRMSDFKSNEYASLLGGRAFEPAEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMG
LVNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLGVYVMCEIPNNVIMIDEFSQLFDGFSIGSNDLTQLTLGIDRDSA
LVAHVFDERDPGVMRLIASVVEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSILKITLRVLEMEKEIEKP
>Mature_799_residues
TWIRWFSAITIDDVPLVGGKNASLGEMYRELRPLGVKIPNGFAITAEAYWYLVRSAGVLDEMRRTLAKLHKEDVDDLALR
GHRVRELICGAPLPADLAAEITAAYRRLCIEYGENCDVAVRSSATAEDLPTASFAGQQETYLNIRGEVQLLDACRRCFAS
LFTDRAISYRIDQGFDHFKVGLSIGVMKMVRSDRASSGVIFTLDTETGFRDTVLVTGAWGLGENVVQGAVNPDEFTVFKP
PLRQGFRPIVRRKLGEKKIKMIYGTGTSKHLTKNVEVPREERRHYCLADAEVLELARQALLIEEHYSKRAGHDVPMDIEW
AKDGVSGELFIVQARPETVESQKRLDFLETYVLERKGEVLVTGTSVGGKIAVGPARVITDVHKLNEFSPGEVLVADTTTP
DWEPVMKTAAAIVTNKGGRTCHAAIVSRELGIPSVVGTGDGTGRIAPGREITVSCAEGDVGRIYDGALPFRIERVDLSGM
RRPRTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKVHPMALVHPERVEDEASRREIGRLTAGYPDPPAYFVEK
LAEGIGTIAAAFHPKPVIVRMSDFKSNEYASLLGGRAFEPAEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGL
VNVIPMIPFCRRIVEAEKVLAEMAANGLVRGENGLGVYVMCEIPNNVIMIDEFSQLFDGFSIGSNDLTQLTLGIDRDSAL
VAHVFDERDPGVMRLIASVVEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNPDSILKITLRVLEMEKEIEKP

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=794, Percent_Identity=52.1410579345088, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1789193, Length=413, Percent_Identity=28.0871670702179, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788756, Length=417, Percent_Identity=24.7002398081535, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI48994992, Length=391, Percent_Identity=25.8312020460358, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788726, Length=391, Percent_Identity=26.8542199488491, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17564524, Length=233, Percent_Identity=27.8969957081545, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 88596; Mature: 88465

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWIRWFSAITIDDVPLVGGKNASLGEMYRELRPLGVKIPNGFAITAEAYWYLVRSAGVL
CCHHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHHCCHH
DEMRRTLAKLHKEDVDDLALRGHRVRELICGAPLPADLAAEITAAYRRLCIEYGENCDVA
HHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VRSSATAEDLPTASFAGQQETYLNIRGEVQLLDACRRCFASLFTDRAISYRIDQGFDHFK
EECCCCHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCHHH
VGLSIGVMKMVRSDRASSGVIFTLDTETGFRDTVLVTGAWGLGENVVQGAVNPDEFTVFK
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEC
PPLRQGFRPIVRRKLGEKKIKMIYGTGTSKHLTKNVEVPREERRHYCLADAEVLELARQA
CHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCHHHCCCEEECCHHHHHHHHHH
LLIEEHYSKRAGHDVPMDIEWAKDGVSGELFIVQARPETVESQKRLDFLETYVLERKGEV
HHHHHHHHHHCCCCCCCEEHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCE
LVTGTSVGGKIAVGPARVITDVHKLNEFSPGEVLVADTTTPDWEPVMKTAAAIVTNKGGR
EEEECCCCCEEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
TCHAAIVSRELGIPSVVGTGDGTGRIAPGREITVSCAEGDVGRIYDGALPFRIERVDLSG
EEHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEEEEEECCCC
MRRPRTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKVHPMALVHPERVEDEAS
CCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHEEEEEECCEEEEEEEEECCHHHCCHHH
RREIGRLTAGYPDPPAYFVEKLAEGIGTIAAAFHPKPVIVRMSDFKSNEYASLLGGRAFE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCC
PAEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGLVNVIPMIPFCRRIVEAEKV
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAEMAANGLVRGENGLGVYVMCEIPNNVIMIDEFSQLFDGFSIGSNDLTQLTLGIDRDSA
HHHHHHCCEEECCCCCEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCC
LVAHVFDERDPGVMRLIASVVEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNP
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DSILKITLRVLEMEKEIEKP
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TWIRWFSAITIDDVPLVGGKNASLGEMYRELRPLGVKIPNGFAITAEAYWYLVRSAGVL
CHHHEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCEECCCCEEEEHHHHHHHHHHCCHH
DEMRRTLAKLHKEDVDDLALRGHRVRELICGAPLPADLAAEITAAYRRLCIEYGENCDVA
HHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VRSSATAEDLPTASFAGQQETYLNIRGEVQLLDACRRCFASLFTDRAISYRIDQGFDHFK
EECCCCHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHEEEECCCCCHHH
VGLSIGVMKMVRSDRASSGVIFTLDTETGFRDTVLVTGAWGLGENVVQGAVNPDEFTVFK
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEC
PPLRQGFRPIVRRKLGEKKIKMIYGTGTSKHLTKNVEVPREERRHYCLADAEVLELARQA
CHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCHHHCCCEEECCHHHHHHHHHH
LLIEEHYSKRAGHDVPMDIEWAKDGVSGELFIVQARPETVESQKRLDFLETYVLERKGEV
HHHHHHHHHHCCCCCCCEEHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCE
LVTGTSVGGKIAVGPARVITDVHKLNEFSPGEVLVADTTTPDWEPVMKTAAAIVTNKGGR
EEEECCCCCEEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
TCHAAIVSRELGIPSVVGTGDGTGRIAPGREITVSCAEGDVGRIYDGALPFRIERVDLSG
EEHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEEEEEECCCC
MRRPRTRIMMNLGNPEEAFSLCRIPNDGVGLARMEFIITNHIKVHPMALVHPERVEDEAS
CCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHEEEEEECCEEEEEEEEECCHHHCCHHH
RREIGRLTAGYPDPPAYFVEKLAEGIGTIAAAFHPKPVIVRMSDFKSNEYASLLGGRAFE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCCCC
PAEENPMIGFRGASRYYDERYREGFALECRALKRVRDEMGLVNVIPMIPFCRRIVEAEKV
CCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAEMAANGLVRGENGLGVYVMCEIPNNVIMIDEFSQLFDGFSIGSNDLTQLTLGIDRDSA
HHHHHHCCEEECCCCCEEEEEEECCCCEEEEECHHHHHCCCCCCCCCCEEEEEECCCCCC
LVAHVFDERDPGVMRLIASVVEGARRNHRHSGICGQAPSDYPEFAAFLVREGIDSISLNP
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
DSILKITLRVLEMEKEIEKP
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]