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The map label for this gene is nifK [H]

Identifier: 78221884

GI number: 78221884

Start: 727384

End: 728853

Strand: Direct

Name: nifK [H]

Synonym: Gmet_0664

Alternate gene names: 78221884

Gene position: 727384-728853 (Clockwise)

Preceding gene: 78221883

Following gene: 78221885

Centisome position: 18.2

GC content: 61.84

Gene sequence:

>1470_bases
ATGGCAAACAATCTAGGGCTAGCTGTCAAGCCGGTGACCGAGACCCCCGTCGAGGAGATCGAGAGAGTCGCCGCCTGGAT
CAACACCGAGGAGTACAGGGACCTCAACTTCAAGCGGGAGGCGTTGGTGGTCGCGCCGCCCCATGCCTGCCAGCCCCTGG
GGGCCGAACTGGCGGCCCACGCCTTCGAAGGAAGCCTCCCCTTCGTCCACGGCTCCCAGGGGTGCGCATCCTACTACCGC
TCCACCCTGAACCGCCATTTCCGCGAACCTGCCCCGGCCGTCTCCGATTCCATGACCGAGGACGGCGCCGTGTTCGGCGG
CCAGAACAACCTCCACGAGGGTCTGGAGAACGCCATTGCCCTCTACAAGCCGAAGATGGTCTCCATCTTTACCTCGTGCA
TGCCGGAGATCATTGGCGACGACCTGTCCGCGTTCATCAAGAATGCCCGCAACAAGGGGATCGTCCCTGCGGACATGCCG
GTCCCCTATGCCAACACCCCGAGCTTCAGCGGCTCCCACGTCCACGGCTACGACGCCATGCTCCTCTCCATCCTCCAGGC
CCTGACCGAGGGGAAGAAGGTGGAGGGGCGCTGCACCGGCAAGATCAACCTGCTTCCCGGCTTTGACGCCAACACCGGCA
ACTTTAGGGAGTACAAGCGAATCTTCGAGGCTTTCGGCGTGCCGTACACCATCCTCGGCGACATCTCCGACGTCTTCGAT
TCGCCTCTGGACGGCACCTACCGCATCTATCCCGGCGGCACCAGGCTGGACGATGCTGCCGACTCCATCAACGGCAAGGC
CACCATCAGCCTCGGCCCCTATTCGGCCACCAAGACCTATGCCTGGGCCAAGGACAACTATGCCGGCAAGCACACGGCGC
TCCCCATGCCGCTGGGGATTGCCAAGACCGATGCCTTCCTCATGAAGATCGCCGAGGTCGCCGGCAAGCCGGTACCCGAG
AGCTTCAAGGCCGAACGGGGGAGGGCGGTGGATGCCATGACCGACGCCCACCAGTACATGCACGGCAAGAAGTTCGCCCT
CTACGGCGACCCGGACCAGCTCATCGGCTACGTCAGCTTCCTCTTGGAGATGGGGGCGGTGCCGTACCACATTCTCTGCA
GCAAGGGGAGCAAGAAGGTGGAAAAGGAGATCCAGGCGCTCCTCGATTTGTCCCCGTACGGGAAGCAGGGGAAGATTTAC
ATGGGCAAGGACCTCTGGCACATGAGGAGTCTCATCATGACCGACCCCGTGGACGCCATCATCGGGGACACCCACGGCAA
GTTCATGGCCCGCGACGCGAAGATACCGCTGTTCCGCTTCGGCTTCCCTGTCCTTGACCGGGTGAACCTGCACCGCTACC
CGATCATCGGCTACCAGGGGGTAATCAACATGGTGACGACCATCTGCAACAAATTTATCGACATCACCGACGAGACTTGT
GAGGATAGACAGTTTGAGATGATGCGCTAA

Upstream 100 bases:

>100_bases
TAAAACACAATAAATTTTTGCTTTTCTCTGTTGCTCAGTTGCTCTGTGTTGAAAATTTGCGACTGTGAACGGATGAGTTC
CGGTCGAAAGGAGATATATC

Downstream 100 bases:

>100_bases
CCGGCTGTAATTATTAAATATCGGTACGAAGGCCAGTGGCTATATTCTAGACGAAAATTTATAGAATACAGAGTCACTGG
CCTTTTATTTATGGTTCGGT

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MANNLGLAVKPVTETPVEEIERVAAWINTEEYRDLNFKREALVVAPPHACQPLGAELAAHAFEGSLPFVHGSQGCASYYR
STLNRHFREPAPAVSDSMTEDGAVFGGQNNLHEGLENAIALYKPKMVSIFTSCMPEIIGDDLSAFIKNARNKGIVPADMP
VPYANTPSFSGSHVHGYDAMLLSILQALTEGKKVEGRCTGKINLLPGFDANTGNFREYKRIFEAFGVPYTILGDISDVFD
SPLDGTYRIYPGGTRLDDAADSINGKATISLGPYSATKTYAWAKDNYAGKHTALPMPLGIAKTDAFLMKIAEVAGKPVPE
SFKAERGRAVDAMTDAHQYMHGKKFALYGDPDQLIGYVSFLLEMGAVPYHILCSKGSKKVEKEIQALLDLSPYGKQGKIY
MGKDLWHMRSLIMTDPVDAIIGDTHGKFMARDAKIPLFRFGFPVLDRVNLHRYPIIGYQGVINMVTTICNKFIDITDETC
EDRQFEMMR

Sequences:

>Translated_489_residues
MANNLGLAVKPVTETPVEEIERVAAWINTEEYRDLNFKREALVVAPPHACQPLGAELAAHAFEGSLPFVHGSQGCASYYR
STLNRHFREPAPAVSDSMTEDGAVFGGQNNLHEGLENAIALYKPKMVSIFTSCMPEIIGDDLSAFIKNARNKGIVPADMP
VPYANTPSFSGSHVHGYDAMLLSILQALTEGKKVEGRCTGKINLLPGFDANTGNFREYKRIFEAFGVPYTILGDISDVFD
SPLDGTYRIYPGGTRLDDAADSINGKATISLGPYSATKTYAWAKDNYAGKHTALPMPLGIAKTDAFLMKIAEVAGKPVPE
SFKAERGRAVDAMTDAHQYMHGKKFALYGDPDQLIGYVSFLLEMGAVPYHILCSKGSKKVEKEIQALLDLSPYGKQGKIY
MGKDLWHMRSLIMTDPVDAIIGDTHGKFMARDAKIPLFRFGFPVLDRVNLHRYPIIGYQGVINMVTTICNKFIDITDETC
EDRQFEMMR
>Mature_488_residues
ANNLGLAVKPVTETPVEEIERVAAWINTEEYRDLNFKREALVVAPPHACQPLGAELAAHAFEGSLPFVHGSQGCASYYRS
TLNRHFREPAPAVSDSMTEDGAVFGGQNNLHEGLENAIALYKPKMVSIFTSCMPEIIGDDLSAFIKNARNKGIVPADMPV
PYANTPSFSGSHVHGYDAMLLSILQALTEGKKVEGRCTGKINLLPGFDANTGNFREYKRIFEAFGVPYTILGDISDVFDS
PLDGTYRIYPGGTRLDDAADSINGKATISLGPYSATKTYAWAKDNYAGKHTALPMPLGIAKTDAFLMKIAEVAGKPVPES
FKAERGRAVDAMTDAHQYMHGKKFALYGDPDQLIGYVSFLLEMGAVPYHILCSKGSKKVEKEIQALLDLSPYGKQGKIYM
GKDLWHMRSLIMTDPVDAIIGDTHGKFMARDAKIPLFRFGFPVLDRVNLHRYPIIGYQGVINMVTTICNKFIDITDETCE
DRQFEMMR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53886; Mature: 53755

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNLGLAVKPVTETPVEEIERVAAWINTEEYRDLNFKREALVVAPPHACQPLGAELAAH
CCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
AFEGSLPFVHGSQGCASYYRSTLNRHFREPAPAVSDSMTEDGAVFGGQNNLHEGLENAIA
HHCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEECCCCHHHHHHHHHHH
LYKPKMVSIFTSCMPEIIGDDLSAFIKNARNKGIVPADMPVPYANTPSFSGSHVHGYDAM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LLSILQALTEGKKVEGRCTGKINLLPGFDANTGNFREYKRIFEAFGVPYTILGDISDVFD
HHHHHHHHHCCCCCCCEECEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHC
SPLDGTYRIYPGGTRLDDAADSINGKATISLGPYSATKTYAWAKDNYAGKHTALPMPLGI
CCCCCEEEEECCCCCCCHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCC
AKTDAFLMKIAEVAGKPVPESFKAERGRAVDAMTDAHQYMHGKKFALYGDPDQLIGYVSF
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LLEMGAVPYHILCSKGSKKVEKEIQALLDLSPYGKQGKIYMGKDLWHMRSLIMTDPVDAI
HHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHH
IGDTHGKFMARDAKIPLFRFGFPVLDRVNLHRYPIIGYQGVINMVTTICNKFIDITDETC
HCCCCCCEEECCCCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EDRQFEMMR
CCCHHHCCC
>Mature Secondary Structure 
ANNLGLAVKPVTETPVEEIERVAAWINTEEYRDLNFKREALVVAPPHACQPLGAELAAH
CCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
AFEGSLPFVHGSQGCASYYRSTLNRHFREPAPAVSDSMTEDGAVFGGQNNLHEGLENAIA
HHCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEECCCCHHHHHHHHHHH
LYKPKMVSIFTSCMPEIIGDDLSAFIKNARNKGIVPADMPVPYANTPSFSGSHVHGYDAM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LLSILQALTEGKKVEGRCTGKINLLPGFDANTGNFREYKRIFEAFGVPYTILGDISDVFD
HHHHHHHHHCCCCCCCEECEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHC
SPLDGTYRIYPGGTRLDDAADSINGKATISLGPYSATKTYAWAKDNYAGKHTALPMPLGI
CCCCCEEEEECCCCCCCHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCC
AKTDAFLMKIAEVAGKPVPESFKAERGRAVDAMTDAHQYMHGKKFALYGDPDQLIGYVSF
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LLEMGAVPYHILCSKGSKKVEKEIQALLDLSPYGKQGKIYMGKDLWHMRSLIMTDPVDAI
HHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHCCCHHHH
IGDTHGKFMARDAKIPLFRFGFPVLDRVNLHRYPIIGYQGVINMVTTICNKFIDITDETC
HCCCCCCEEECCCCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
EDRQFEMMR
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]