Click here to switch to the map view.

The map label for this gene is nifD [H]

Identifier: 78221883

GI number: 78221883

Start: 725802

End: 727247

Strand: Direct

Name: nifD [H]

Synonym: Gmet_0663

Alternate gene names: 78221883

Gene position: 725802-727247 (Clockwise)

Preceding gene: 78221882

Following gene: 78221884

Centisome position: 18.16

GC content: 61.13

Gene sequence:

>1446_bases
ATGTCAAAGGCAATCAGAAATATTGACGGGATAACCAAGGCGTCGACCCAGGAGATGATCGACAAGGTCTTGGAGGTCTA
TCCGGAAAAGGGGCGGAAGAAGCGGGCGCCCCACCTGGCTCCCAACGACCAGGCGAGTTGCAGCGCTTGCGTCAAATCCA
ACAAAAAGACAGTGCCCGGCGTCATGAGTGCCCGGGGCTGCGCCTATGCCGGCGCCAAGGGAGTTGTCTGGGGGCCGATC
CGCGACATGGTCCACGTCTCCCACGGCCCGGTGGGGTGCGGCTGGTACTCCTGGGGAACCCGGCGCAACCTCATGTCCGG
CAAGCTGGGAGTCGACCAGTTCGGCATGCAGTTCACCTCCGACTTTCAGGAGAAGGACATCGTCTACGGCGGCGACAAGA
AACTGAAGGTGCTGCTCAAGGAGGCGAAGGAGCTCTTCCCCCTGGCCAAGGGGATCTCGGTCCTCTCCGAGTGCCCGGTG
GGGCTCATCGGCGACGATATCAACGCCGTGGCCAAGACGAGTGCCAAGGAGCTGGACATCCCGATCATCCCCTGCAACTG
CGAGGGGTTCCGGGGGGTCTCCCAGTCCCTGGGGCACCATATCTCCAACGACACCATCCGCGACTACATCATCGAGACCC
GCGAGTTCGCCGAGCCCGTGGGCCCCTACGACATCGCCCTCATCGGCGAGTACAACATCGGCGGGGACGCCTGGTCCACA
AAGCCCCTGCTGGAGGAGTGCGGCTTCAACGTCAAGGCGGTCTGGACCGGCGACGGCGAGATGGAGAAGATCGCCGCCAC
CCATCAGGTGCGGCTCAACGTCATCCACTGCTACCGTTCCATGAACTACATGTGCAAGGTGATGGAAGAAAAGTATGGCA
TTCCCTGGATCGAGCTGAACTTTTTCGGACCCACCAAGATCCGGGAATCGCTCCGCAAGCTGGCCGAGCTCTTTGACGAC
ACCATCAAGGAGAAGGTGGAGGCGGTCATCGCCAAGTACGATCCGCAGATGCAGGCGGTGATCGAGGAGGTCCGTCCCCG
GCTCGAAGGGAAGAAGGTGATGCTCTATGTCGGCGGCCTCCGCCCGCGCCACACAATTGGCGCCTATGAGGATCTCGGGA
TGCTCTGTGTCGGCTCCGGCTACGAGTTCGCCCATACCGACGACTACGACCGGACCGCCCCGGAGATGCCCGATGCCACC
GTGGTCTACGACGACGCGTCCGAGTTGGAGATGGAACAGTTCGCCCACGTGCTGAAGCCCGACTTGGTCGCCTCCGGCAT
CAAGGAGAAATACGTCTTCCAGAAGATGGGGCTGCCGTTCCGCCAGATGCACAGCTGGGACTATTCCGGCCCGTACCACG
GCTACAAGGGGTTCCCGATCTTTGCCAGAGACATCGATATGGCAATTAACTCGCCGACGTGGAATTTGGTCAGAAGCCCT
TATTAG

Upstream 100 bases:

>100_bases
CTTCCTTGGAAGCCGCCGACCTGACCTCGAAATCGTTCGCCAGTAGGTTTAACCCATTATATGTAAGGGTGCTGCTTCCT
GTGCCCTTCAAGGAGCTTTT

Downstream 100 bases:

>100_bases
TATTGAAACACAAGCAACTGAGCAACTGAGTAAGTTTAAAACACAATAAATTTTTGCTTTTCTCTGTTGCTCAGTTGCTC
TGTGTTGAAAATTTGCGACT

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSKAIRNIDGITKASTQEMIDKVLEVYPEKGRKKRAPHLAPNDQASCSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNLMSGKLGVDQFGMQFTSDFQEKDIVYGGDKKLKVLLKEAKELFPLAKGISVLSECPV
GLIGDDINAVAKTSAKELDIPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIETREFAEPVGPYDIALIGEYNIGGDAWST
KPLLEECGFNVKAVWTGDGEMEKIAATHQVRLNVIHCYRSMNYMCKVMEEKYGIPWIELNFFGPTKIRESLRKLAELFDD
TIKEKVEAVIAKYDPQMQAVIEEVRPRLEGKKVMLYVGGLRPRHTIGAYEDLGMLCVGSGYEFAHTDDYDRTAPEMPDAT
VVYDDASELEMEQFAHVLKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYKGFPIFARDIDMAINSPTWNLVRSP
Y

Sequences:

>Translated_481_residues
MSKAIRNIDGITKASTQEMIDKVLEVYPEKGRKKRAPHLAPNDQASCSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNLMSGKLGVDQFGMQFTSDFQEKDIVYGGDKKLKVLLKEAKELFPLAKGISVLSECPV
GLIGDDINAVAKTSAKELDIPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIETREFAEPVGPYDIALIGEYNIGGDAWST
KPLLEECGFNVKAVWTGDGEMEKIAATHQVRLNVIHCYRSMNYMCKVMEEKYGIPWIELNFFGPTKIRESLRKLAELFDD
TIKEKVEAVIAKYDPQMQAVIEEVRPRLEGKKVMLYVGGLRPRHTIGAYEDLGMLCVGSGYEFAHTDDYDRTAPEMPDAT
VVYDDASELEMEQFAHVLKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYKGFPIFARDIDMAINSPTWNLVRSP
Y
>Mature_480_residues
SKAIRNIDGITKASTQEMIDKVLEVYPEKGRKKRAPHLAPNDQASCSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPIR
DMVHVSHGPVGCGWYSWGTRRNLMSGKLGVDQFGMQFTSDFQEKDIVYGGDKKLKVLLKEAKELFPLAKGISVLSECPVG
LIGDDINAVAKTSAKELDIPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIETREFAEPVGPYDIALIGEYNIGGDAWSTK
PLLEECGFNVKAVWTGDGEMEKIAATHQVRLNVIHCYRSMNYMCKVMEEKYGIPWIELNFFGPTKIRESLRKLAELFDDT
IKEKVEAVIAKYDPQMQAVIEEVRPRLEGKKVMLYVGGLRPRHTIGAYEDLGMLCVGSGYEFAHTDDYDRTAPEMPDATV
VYDDASELEMEQFAHVLKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYKGFPIFARDIDMAINSPTWNLVRSPY

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53764; Mature: 53633

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAIRNIDGITKASTQEMIDKVLEVYPEKGRKKRAPHLAPNDQASCSACVKSNKKTVPG
CCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNLMSGKLGVDQFGMQFTS
HHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
DFQEKDIVYGGDKKLKVLLKEAKELFPLAKGISVLSECPVGLIGDDINAVAKTSAKELDI
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHCCC
PIIPCNCEGFRGVSQSLGHHISNDTIRDYIIETREFAEPVGPYDIALIGEYNIGGDAWST
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCC
KPLLEECGFNVKAVWTGDGEMEKIAATHQVRLNVIHCYRSMNYMCKVMEEKYGIPWIELN
CHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPTKIRESLRKLAELFDDTIKEKVEAVIAKYDPQMQAVIEEVRPRLEGKKVMLYVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCC
RPRHTIGAYEDLGMLCVGSGYEFAHTDDYDRTAPEMPDATVVYDDASELEMEQFAHVLKP
CCCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCH
DLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYKGFPIFARDIDMAINSPTWNLVRSP
HHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHEECCCCCCCCCCC
Y
C
>Mature Secondary Structure 
SKAIRNIDGITKASTQEMIDKVLEVYPEKGRKKRAPHLAPNDQASCSACVKSNKKTVPG
CCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNLMSGKLGVDQFGMQFTS
HHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHC
DFQEKDIVYGGDKKLKVLLKEAKELFPLAKGISVLSECPVGLIGDDINAVAKTSAKELDI
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHCCC
PIIPCNCEGFRGVSQSLGHHISNDTIRDYIIETREFAEPVGPYDIALIGEYNIGGDAWST
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCC
KPLLEECGFNVKAVWTGDGEMEKIAATHQVRLNVIHCYRSMNYMCKVMEEKYGIPWIELN
CHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPTKIRESLRKLAELFDDTIKEKVEAVIAKYDPQMQAVIEEVRPRLEGKKVMLYVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCC
RPRHTIGAYEDLGMLCVGSGYEFAHTDDYDRTAPEMPDATVVYDDASELEMEQFAHVLKP
CCCCCCCCHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCH
DLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGYKGFPIFARDIDMAINSPTWNLVRSP
HHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEEHHHEECCCCCCCCCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3062178; 3043382; 3322262; 6809876; 3211766; 3322261 [H]