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The map label for this gene is yitJ [H]

Identifier: 78221725

GI number: 78221725

Start: 546867

End: 548684

Strand: Direct

Name: yitJ [H]

Synonym: Gmet_0504

Alternate gene names: 78221725

Gene position: 546867-548684 (Clockwise)

Preceding gene: 78221724

Following gene: 78221726

Centisome position: 13.68

GC content: 67.49

Gene sequence:

>1818_bases
ATGAATTTTCTCGAACGTTTGAACAGCGAAGTCCTCGTGGGGGACGGTGCCATCGGCACCATGCTCTATGCCAAGGGGGT
CTCCCTCGACGCCAACGTGGAGCACCTGAACCTGGTGCGGCCCGAGTTGGTCCTGGAGCTCCACCGAGAGTACCTGGGCG
CCGGGGCGAAGGTTATTGAAACCAACACTTTTGGCGCCAACTGGACGAAACTCTCGGCCATCGGCCTCGACAAGCGGGAG
CGGGAGATCATCCTGCGGGGGGCCCGGCTGGCCCGGGAGGCCGCCGAGGGGACCGATGCCTTCGTGGCCGGCTCCATGGG
GCCCCTGGCAAGGGTTAAGGGGGACGAGCGGGAGCTTTCCCCGGCCGAGACCACGGAGATCTTCCGCCGGCAGGCCCTGG
CCCTGGCCGAGGGGGGTGTTGATCTTCTCATTCTTGAGACCTTCACCGACTTGGCCCAGATTCTCTGCGCCCTCTCGGCG
GCCCGAGAAACGGGGCTCCCGGTCGTGGCGAACATGGCGTTTCTGGAGAACGGCCGCCTTGCCGGCGGGGCTGACGTGGA
ACGGGCCGCGCGGGAACTGACCGCCGCCGGGGCAAGCGTCGTGGGAGCCAACTGCGGCGCCGGCCCCCTGGAGGTCCTCG
GCACCATCCGGCGCATGGCAGGCGCCACGGCCCTGCCGATAGCGGCGTACCCCAACAGCGGTTTCCCCGAGTACGTGAAC
GGCCGTCACGTCTATCGCACCACCCCCGACTACTTCGCCGAGCGGGCCGCCGAGATGGTGGCTGCCGGGGCGGCCCTCGT
GGGGGGGTGCTGCGGCACCACGCCGGAGCATATCCTCCGTCTGGCGGAGCGGCTGGCGGGGCTGCGCCCCGCGGTCCGGA
ACGTGGCGGTTCCGGCTGTCTCGGTCGAGGAACGGCGGGGCGAGAGCACGGAAGCCGAGGGATTCCTGGCCCGCTGGGGG
GAGGAGCCGGTGGTCACCGTGGAACTGGACCCTCCCAAGGGCTTCGACTGCGGGAAGGTTATCGAGGGGAGCCGGGTCCT
GAAGGGGGCCGGTGCCGATGCCATCAATATCGCCGAAAACCCCCTGGCCCGCATCCGGATGGGGAATATCGCCCTGGGGC
ATCTCATCCAGCAAGAGGTGGGGATCGAGGTCATCGTCCACGTCACCTGCCGGGACCGCAACCTCCTGGGGCTCCAGTCG
GACCTCATGGGGGCGAGCCTCCTGGGCATCCGGAGCATCCTCGCCGTCACCGGGGACCCGGCCCGGATCGGGGAGCAGGC
CGGGGCCTCGTCGGTCTACGACCTGAATTCTTTCACTCTCATCAAGCTCCTGGCCGACCTGAACGCAGGGGTGAACGCCC
TTGGCAACCCCCTTGGCCGGGGTGCAGGGTTTGCCATCGGCTGTGTCTTCAACCCCAACAGCCAGCGGATGGAGGTCCAG
GCGGAACGCCTCGCCAAGAAGGTTGCCAACGGGGCCCGCTTCGTCCAGACCCAGCCCCTCTACGACCTGGCACGTCTCGA
CGAGATGCTGGAACGGACCTCCCCTCTGGGGATTCCGGTGCTGCCGGGAATCCTTCCGCTGGTCAGTGAGCGCAACTGCG
AGTTCCTCCACAACGAAGTGCCGGGGATCGTGATTCCCGAGGATATCCGGGCCCGGATGCGGGGGAAAGAGAAGGACGAG
GGAGTCCGGGAAGGGCTCGCCATCGCCCGGGAGTTTATCGACGCCGTCCGGGGCCGGGTCGGCGGCTTCTATCTCATGCC
CCCTTTCGGCCGTTACGAGATCGCCGTTGAACTGGTGCGGCACATCAAGGGGAAATAA

Upstream 100 bases:

>100_bases
CCCCAGGTGGAACCCCACCTCTACGAGTTGAAGGGGGTCATGTCCCGCAAGAAGCAGATGGTGCCGCATCTCTTGAAAGT
ACTGAGGAAACTCTAGAGCG

Downstream 100 bases:

>100_bases
ACGGTTTCCCTTGTTGTCACCACAGGAGGTACCCATGCGTATCCAGCGAGTTGCATCGTTTTTCCTAATGCTTCTTTTTC
TTGCCGGCTGCCGCAACACC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MNFLERLNSEVLVGDGAIGTMLYAKGVSLDANVEHLNLVRPELVLELHREYLGAGAKVIETNTFGANWTKLSAIGLDKRE
REIILRGARLAREAAEGTDAFVAGSMGPLARVKGDERELSPAETTEIFRRQALALAEGGVDLLILETFTDLAQILCALSA
ARETGLPVVANMAFLENGRLAGGADVERAARELTAAGASVVGANCGAGPLEVLGTIRRMAGATALPIAAYPNSGFPEYVN
GRHVYRTTPDYFAERAAEMVAAGAALVGGCCGTTPEHILRLAERLAGLRPAVRNVAVPAVSVEERRGESTEAEGFLARWG
EEPVVTVELDPPKGFDCGKVIEGSRVLKGAGADAINIAENPLARIRMGNIALGHLIQQEVGIEVIVHVTCRDRNLLGLQS
DLMGASLLGIRSILAVTGDPARIGEQAGASSVYDLNSFTLIKLLADLNAGVNALGNPLGRGAGFAIGCVFNPNSQRMEVQ
AERLAKKVANGARFVQTQPLYDLARLDEMLERTSPLGIPVLPGILPLVSERNCEFLHNEVPGIVIPEDIRARMRGKEKDE
GVREGLAIAREFIDAVRGRVGGFYLMPPFGRYEIAVELVRHIKGK

Sequences:

>Translated_605_residues
MNFLERLNSEVLVGDGAIGTMLYAKGVSLDANVEHLNLVRPELVLELHREYLGAGAKVIETNTFGANWTKLSAIGLDKRE
REIILRGARLAREAAEGTDAFVAGSMGPLARVKGDERELSPAETTEIFRRQALALAEGGVDLLILETFTDLAQILCALSA
ARETGLPVVANMAFLENGRLAGGADVERAARELTAAGASVVGANCGAGPLEVLGTIRRMAGATALPIAAYPNSGFPEYVN
GRHVYRTTPDYFAERAAEMVAAGAALVGGCCGTTPEHILRLAERLAGLRPAVRNVAVPAVSVEERRGESTEAEGFLARWG
EEPVVTVELDPPKGFDCGKVIEGSRVLKGAGADAINIAENPLARIRMGNIALGHLIQQEVGIEVIVHVTCRDRNLLGLQS
DLMGASLLGIRSILAVTGDPARIGEQAGASSVYDLNSFTLIKLLADLNAGVNALGNPLGRGAGFAIGCVFNPNSQRMEVQ
AERLAKKVANGARFVQTQPLYDLARLDEMLERTSPLGIPVLPGILPLVSERNCEFLHNEVPGIVIPEDIRARMRGKEKDE
GVREGLAIAREFIDAVRGRVGGFYLMPPFGRYEIAVELVRHIKGK
>Mature_605_residues
MNFLERLNSEVLVGDGAIGTMLYAKGVSLDANVEHLNLVRPELVLELHREYLGAGAKVIETNTFGANWTKLSAIGLDKRE
REIILRGARLAREAAEGTDAFVAGSMGPLARVKGDERELSPAETTEIFRRQALALAEGGVDLLILETFTDLAQILCALSA
ARETGLPVVANMAFLENGRLAGGADVERAARELTAAGASVVGANCGAGPLEVLGTIRRMAGATALPIAAYPNSGFPEYVN
GRHVYRTTPDYFAERAAEMVAAGAALVGGCCGTTPEHILRLAERLAGLRPAVRNVAVPAVSVEERRGESTEAEGFLARWG
EEPVVTVELDPPKGFDCGKVIEGSRVLKGAGADAINIAENPLARIRMGNIALGHLIQQEVGIEVIVHVTCRDRNLLGLQS
DLMGASLLGIRSILAVTGDPARIGEQAGASSVYDLNSFTLIKLLADLNAGVNALGNPLGRGAGFAIGCVFNPNSQRMEVQ
AERLAKKVANGARFVQTQPLYDLARLDEMLERTSPLGIPVLPGILPLVSERNCEFLHNEVPGIVIPEDIRARMRGKEKDE
GVREGLAIAREFIDAVRGRVGGFYLMPPFGRYEIAVELVRHIKGK

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=323, Percent_Identity=29.7213622291022, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI13162290, Length=310, Percent_Identity=32.9032258064516, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI157266337, Length=314, Percent_Identity=32.8025477707006, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1790450, Length=323, Percent_Identity=29.4117647058824, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1786456, Length=305, Percent_Identity=31.1475409836066, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535405, Length=322, Percent_Identity=29.1925465838509, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 64561; Mature: 64561

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLERLNSEVLVGDGAIGTMLYAKGVSLDANVEHLNLVRPELVLELHREYLGAGAKVIE
CCHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
TNTFGANWTKLSAIGLDKREREIILRGARLAREAAEGTDAFVAGSMGPLARVKGDERELS
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCC
PAETTEIFRRQALALAEGGVDLLILETFTDLAQILCALSAARETGLPVVANMAFLENGRL
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGGADVERAARELTAAGASVVGANCGAGPLEVLGTIRRMAGATALPIAAYPNSGFPEYVN
CCCCHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GRHVYRTTPDYFAERAAEMVAAGAALVGGCCGTTPEHILRLAERLAGLRPAVRNVAVPAV
CCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCE
SVEERRGESTEAEGFLARWGEEPVVTVELDPPKGFDCGKVIEGSRVLKGAGADAINIAEN
EHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCC
PLARIRMGNIALGHLIQQEVGIEVIVHVTCRDRNLLGLQSDLMGASLLGIRSILAVTGDP
CHHHEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
ARIGEQAGASSVYDLNSFTLIKLLADLNAGVNALGNPLGRGAGFAIGCVFNPNSQRMEVQ
HHHHHHCCCCCEEECCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCCHHHHH
AERLAKKVANGARFVQTQPLYDLARLDEMLERTSPLGIPVLPGILPLVSERNCEFLHNEV
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHCCC
PGIVIPEDIRARMRGKEKDEGVREGLAIAREFIDAVRGRVGGFYLMPPFGRYEIAVELVR
CCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
HIKGK
HHCCC
>Mature Secondary Structure
MNFLERLNSEVLVGDGAIGTMLYAKGVSLDANVEHLNLVRPELVLELHREYLGAGAKVIE
CCHHHHCCCCEEEECCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
TNTFGANWTKLSAIGLDKREREIILRGARLAREAAEGTDAFVAGSMGPLARVKGDERELS
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCC
PAETTEIFRRQALALAEGGVDLLILETFTDLAQILCALSAARETGLPVVANMAFLENGRL
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
AGGADVERAARELTAAGASVVGANCGAGPLEVLGTIRRMAGATALPIAAYPNSGFPEYVN
CCCCHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCC
GRHVYRTTPDYFAERAAEMVAAGAALVGGCCGTTPEHILRLAERLAGLRPAVRNVAVPAV
CCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCE
SVEERRGESTEAEGFLARWGEEPVVTVELDPPKGFDCGKVIEGSRVLKGAGADAINIAEN
EHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCC
PLARIRMGNIALGHLIQQEVGIEVIVHVTCRDRNLLGLQSDLMGASLLGIRSILAVTGDP
CHHHEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCH
ARIGEQAGASSVYDLNSFTLIKLLADLNAGVNALGNPLGRGAGFAIGCVFNPNSQRMEVQ
HHHHHHCCCCCEEECCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCCHHHHH
AERLAKKVANGARFVQTQPLYDLARLDEMLERTSPLGIPVLPGILPLVSERNCEFLHNEV
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHCCC
PGIVIPEDIRARMRGKEKDEGVREGLAIAREFIDAVRGRVGGFYLMPPFGRYEIAVELVR
CCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
HIKGK
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]