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The map label for this gene is nuoE [H]

Identifier: 78221379

GI number: 78221379

Start: 185865

End: 186386

Strand: Direct

Name: nuoE [H]

Synonym: Gmet_0154

Alternate gene names: 78221379

Gene position: 185865-186386 (Clockwise)

Preceding gene: 78221378

Following gene: 78221380

Centisome position: 4.65

GC content: 66.09

Gene sequence:

>522_bases
ATGCTTACTAACCGTCTGAAATCTGATCTCCAGGCCCGCATCGCCCACGCCGTCACCAACCGCGAGGCGGCGGTGGACGT
GATAAAGGAGCTGCAGCGGCACTACGGCTGGCTCACCGACGAGGCGGTGGCCGAGGCGGCAGGGCTCCTGGGGCTCACGC
CGCTCCAGGTGGAGGAGCTGGCCACTTTCTACGAAATGATCTACCGCCGGCCGGTAGGTAAGCGGGTGGTCCACGTCTGC
GACTCCATCTCCTGCTGGGCCATGGGAGGAGAGAGCCTCATGGCGCACCTGGCGGCGAAGCTCGGCGTAGAACCCGGCGG
GACCACGGCGGACGGGATGTTCACGCTCCTTCCCTGCTGCTGCCTCGGCAACTGCGGCGAGGCGCCGACGCTGATGATCG
GCGACACCCTCTATGGCCGGGTGACGCCGGAGCGGGGAGAGGAAATCCTGAGCAACGCGCGGGAAACCGCAGCAACCGTG
CCAGAAGGACCCGGCACACCACAAGAGGAGGAGAACGTATGA

Upstream 100 bases:

>100_bases
CTCCCCCTCATGAGCCGAGGGTGGCTCGTGGCGGATTTTCTCGCCATCATCGGGTCCATCGACTTTGTACTGGCGGATCT
GGACCGGTGAAAAGGTTTTT

Downstream 100 bases:

>100_bases
AAAAGGGAATTATCATCTCCATCAGCCTGCTGGCCACGGCGGCGTTCGCCACGGCCGGCCTCGCCGACACCAAGAAGGGG
GGGAAGATCGACGGCGCCAA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MLTNRLKSDLQARIAHAVTNREAAVDVIKELQRHYGWLTDEAVAEAAGLLGLTPLQVEELATFYEMIYRRPVGKRVVHVC
DSISCWAMGGESLMAHLAAKLGVEPGGTTADGMFTLLPCCCLGNCGEAPTLMIGDTLYGRVTPERGEEILSNARETAATV
PEGPGTPQEEENV

Sequences:

>Translated_173_residues
MLTNRLKSDLQARIAHAVTNREAAVDVIKELQRHYGWLTDEAVAEAAGLLGLTPLQVEELATFYEMIYRRPVGKRVVHVC
DSISCWAMGGESLMAHLAAKLGVEPGGTTADGMFTLLPCCCLGNCGEAPTLMIGDTLYGRVTPERGEEILSNARETAATV
PEGPGTPQEEENV
>Mature_173_residues
MLTNRLKSDLQARIAHAVTNREAAVDVIKELQRHYGWLTDEAVAEAAGLLGLTPLQVEELATFYEMIYRRPVGKRVVHVC
DSISCWAMGGESLMAHLAAKLGVEPGGTTADGMFTLLPCCCLGNCGEAPTLMIGDTLYGRVTPERGEEILSNARETAATV
PEGPGTPQEEENV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=143, Percent_Identity=37.7622377622378, Blast_Score=100, Evalue=7e-22,
Organism=Escherichia coli, GI1788621, Length=145, Percent_Identity=40, Blast_Score=128, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17561328, Length=132, Percent_Identity=37.8787878787879, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859877, Length=144, Percent_Identity=35.4166666666667, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355353, Length=133, Percent_Identity=36.0902255639098, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18628; Mature: 18628

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNRLKSDLQARIAHAVTNREAAVDVIKELQRHYGWLTDEAVAEAAGLLGLTPLQVEEL
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
ATFYEMIYRRPVGKRVVHVCDSISCWAMGGESLMAHLAAKLGVEPGGTTADGMFTLLPCC
HHHHHHHHHCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CLGNCGEAPTLMIGDTLYGRVTPERGEEILSNARETAATVPEGPGTPQEEENV
HCCCCCCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTNRLKSDLQARIAHAVTNREAAVDVIKELQRHYGWLTDEAVAEAAGLLGLTPLQVEEL
CCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
ATFYEMIYRRPVGKRVVHVCDSISCWAMGGESLMAHLAAKLGVEPGGTTADGMFTLLPCC
HHHHHHHHHCCHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CLGNCGEAPTLMIGDTLYGRVTPERGEEILSNARETAATVPEGPGTPQEEENV
HCCCCCCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]