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The map label for this gene is gatB

Identifier: 78221300

GI number: 78221300

Start: 89602

End: 91041

Strand: Reverse

Name: gatB

Synonym: Gmet_0073

Alternate gene names: 78221300

Gene position: 91041-89602 (Counterclockwise)

Preceding gene: 78221301

Following gene: 78221299

Centisome position: 2.28

GC content: 65.28

Gene sequence:

>1440_bases
ATGAACTACCAAGCCGTCATCGGCCTTGAAGTCCACGTCCAGCTGAAAACCGACACCAAGATCTTCTGCGGCTGCTCCAC
CCAGTTCGGTGCCAGCCCCAACTCCCAGACCTGCCCGGTCTGCCTCGGGATGCCCGGCGCCCTGCCGGTCCTGAACCGGA
AGGTGGTGGAGTTCGCCATCAAGGCCGGTCTCGCCACCAACTGCCGGATCGCGCCGCGCAGCGTCTTCGCCCGGAAGAAC
TACTTCTACCCGGACCTCCCCAAGGGGTACCAGATCAGCCAGTTCGAACTCCCCATCTGCCAGAACGGCCACCTGGACAT
CGAGGTGGAGGGTGAGACTAAGCGGATCGGCATCACCCGCATCCACATGGAGGAGGACGCCGGCAAGCTGGTCCACAGCG
ACATCCCCGGCCTGGGAAGCGGCTCCGGGGTGGACCTGAACCGGGCCTGCACGCCGCTCCTGGAAATCGTGTCGGAGCCG
GACATCCGCAGCGCCGACGAAGCGGTGGCCTACCTGAAGAAGCTCCATCAGATCGTGGTCTACCTCGGCATCTGCGACGG
GAACATGGAGGAGGGGAGCTTCCGCTGCGACGCCAACGTGTCGGTGATGCCGGTGGGCTCCACGACTTTCGGGACCCGCA
CCGAGACCAAGAACGTCAACTCCTTCCGCTTCGTGAAACAGGCCATCGAGTACGAGATCGAGCGGCAGATCGAGCTCATC
GAGGAGGGGGGGAAGGTGGTCCAGGAGACCCGCCTCTTCAACCCGGACAGCGGCGAAACCCGCTCCATGCGCGGCAAGGA
GGAGGCCCACGACTACCGCTACTTCCCCGATCCCGATCTCGTGCCGCTCGTCATCTCCAACGACTGGGTGGCCGACGTGG
CCCTGTCGCTCCCCGAACTCCCCGAGGCCCGGCGCAATCGCTACCGCTCCGACTTGGGGCTTTCCGACTACGACGCCGAG
GTCCTGACCGCGACGCGGGAAATGGCCGAGTACTTCGAAGCGTGCCTCGCCGCCGGCGCCCCGGCCAAGGGGGCCGCCAA
CTGGGTGATGGGTGAGGTGACTCGCTCCCTCAACGAGGCGGGCACGGGAATCGCCGACTGCCCGGTGACGCCGGAGCGCC
TCACGGCCCTGCTCCAGCTCATCGAGAAGGGGACCATCTCGGGCAAAATCGCCAAGACCGTCTTCGACGAGATGTGGACG
AACGACAAGGCCCCCGAGGCCATCGTGGAGGAGAAGGGGCTCGTCCAGGTCTCCGACACCGGCGCCATCGAGAAGATCAT
CGACGAGATCATGGCCGCCAACATGGGGCAGGTGGAGGAGTTCCGCGGCGGCAAGGAGAAGGTCTTCGGCTTCTTCGTGG
GGCAGGTGATGCGGGCCTCCAAGGGGAAGGCGAACCCGGCGGTGGTGAACGAGCTGTTACTGAAGAAGCTCAAGGGGTGA

Upstream 100 bases:

>100_bases
GCCCTTGGCCAGACCTATTGCCGGTTGCCGACAGCATAAAGAAGTTGGAGAAAAAACAGAAAAAGCTACTATCCGAACTC
CCTGAACCAGGAGAAACCGA

Downstream 100 bases:

>100_bases
GATTCAACTTGCTGTTCTCTGTGTCTCCGTGTCTCTGTGGCAGATTGTGAGGGTTATTTATGTCCTTTAGAACTATCGAA
TGGCGCGACGACAAGGTAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTQFGASPNSQTCPVCLGMPGALPVLNRKVVEFAIKAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQFELPICQNGHLDIEVEGETKRIGITRIHMEEDAGKLVHSDIPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLKKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEYEIERQIELI
EEGGKVVQETRLFNPDSGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVADVALSLPELPEARRNRYRSDLGLSDYDAE
VLTATREMAEYFEACLAAGAPAKGAANWVMGEVTRSLNEAGTGIADCPVTPERLTALLQLIEKGTISGKIAKTVFDEMWT
NDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLLKKLKG

Sequences:

>Translated_479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTQFGASPNSQTCPVCLGMPGALPVLNRKVVEFAIKAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQFELPICQNGHLDIEVEGETKRIGITRIHMEEDAGKLVHSDIPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLKKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEYEIERQIELI
EEGGKVVQETRLFNPDSGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVADVALSLPELPEARRNRYRSDLGLSDYDAE
VLTATREMAEYFEACLAAGAPAKGAANWVMGEVTRSLNEAGTGIADCPVTPERLTALLQLIEKGTISGKIAKTVFDEMWT
NDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLLKKLKG
>Mature_479_residues
MNYQAVIGLEVHVQLKTDTKIFCGCSTQFGASPNSQTCPVCLGMPGALPVLNRKVVEFAIKAGLATNCRIAPRSVFARKN
YFYPDLPKGYQISQFELPICQNGHLDIEVEGETKRIGITRIHMEEDAGKLVHSDIPGLGSGSGVDLNRACTPLLEIVSEP
DIRSADEAVAYLKKLHQIVVYLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEYEIERQIELI
EEGGKVVQETRLFNPDSGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVADVALSLPELPEARRNRYRSDLGLSDYDAE
VLTATREMAEYFEACLAAGAPAKGAANWVMGEVTRSLNEAGTGIADCPVTPERLTALLQLIEKGTISGKIAKTVFDEMWT
NDKAPEAIVEEKGLVQVSDTGAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLLKKLKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=38.2293762575453, Blast_Score=328, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI115534094, Length=475, Percent_Identity=32.8421052631579, Blast_Score=235, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=28.5433070866142, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=35.0202429149798, Blast_Score=286, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_GEOMG (Q39ZK2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383047.1
- ProteinModelPortal:   Q39ZK2
- SMR:   Q39ZK2
- STRING:   Q39ZK2
- GeneID:   3739689
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0073
- NMPDR:   fig|269799.3.peg.136
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q39ZK2
- ProtClustDB:   PRK05477
- BioCyc:   GMET269799:GMET_0073-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52509; Mature: 52509

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQAVIGLEVHVQLKTDTKIFCGCSTQFGASPNSQTCPVCLGMPGALPVLNRKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
KAGLATNCRIAPRSVFARKNYFYPDLPKGYQISQFELPICQNGHLDIEVEGETKRIGITR
HCCCCCCCEECHHHHHHHCCCCCCCCCCCCEECEEECCCCCCCCEEEEECCCCEEEEEEE
IHMEEDAGKLVHSDIPGLGSGSGVDLNRACTPLLEIVSEPDIRSADEAVAYLKKLHQIVV
EEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
YLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEYEIERQIELI
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEGGKVVQETRLFNPDSGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVADVALSLPEL
HHCCHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCHHHHHHHCCCCC
PEARRNRYRSDLGLSDYDAEVLTATREMAEYFEACLAAGAPAKGAANWVMGEVTRSLNEA
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GTGIADCPVTPERLTALLQLIEKGTISGKIAKTVFDEMWTNDKAPEAIVEEKGLVQVSDT
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECC
GAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLLKKLKG
CHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYQAVIGLEVHVQLKTDTKIFCGCSTQFGASPNSQTCPVCLGMPGALPVLNRKVVEFAI
CCCEEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHH
KAGLATNCRIAPRSVFARKNYFYPDLPKGYQISQFELPICQNGHLDIEVEGETKRIGITR
HCCCCCCCEECHHHHHHHCCCCCCCCCCCCEECEEECCCCCCCCEEEEECCCCEEEEEEE
IHMEEDAGKLVHSDIPGLGSGSGVDLNRACTPLLEIVSEPDIRSADEAVAYLKKLHQIVV
EEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEE
YLGICDGNMEEGSFRCDANVSVMPVGSTTFGTRTETKNVNSFRFVKQAIEYEIERQIELI
EEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEGGKVVQETRLFNPDSGETRSMRGKEEAHDYRYFPDPDLVPLVISNDWVADVALSLPEL
HHCCHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCHHHHHHHCCCCC
PEARRNRYRSDLGLSDYDAEVLTATREMAEYFEACLAAGAPAKGAANWVMGEVTRSLNEA
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GTGIADCPVTPERLTALLQLIEKGTISGKIAKTVFDEMWTNDKAPEAIVEEKGLVQVSDT
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECC
GAIEKIIDEIMAANMGQVEEFRGGKEKVFGFFVGQVMRASKGKANPAVVNELLLKKLKG
CHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA