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The map label for this gene is walK [H]

Identifier: 78221238

GI number: 78221238

Start: 15674

End: 17200

Strand: Direct

Name: walK [H]

Synonym: Gmet_0010

Alternate gene names: 78221238

Gene position: 15674-17200 (Clockwise)

Preceding gene: 78221237

Following gene: 78221239

Centisome position: 0.39

GC content: 65.88

Gene sequence:

>1527_bases
ATGAATAACCTCAAAATCCTCATCGTCGATGACGAAGCGGACATCGCCCTCATTCTCAAGCTGCAACTGGAGGACGCCGG
CTACCGCACCACCCGGGCCCGGGATGGCCTGGAAGCCCTGGAGATGCTGGCCCGGGAACACTATGACCTGATTCTCCTGG
ACATCCGCATGCCCCGCCTGGATGGCATTGAGGTGCTGGAGCGGATCCGGCAGGAGTGGCCCGAGCTGGTGGTGGTGATG
ATGACTGCCCACGGCAGCGAGGACATCGCCGTTGAGGCCATGAAGAAGGGGGCGGTGGACTATATCTCCAAGCCCTTCTC
CTCCGATGATATGAAGAAGCGGGTGGAGCGGGCCATCGAGTACAACCGGACCCGGCTCGAAAACGAGCGGCTCCAGCGGG
AGGTGGAGGAAGAGCGCCGGAAGATGGAGGCGATTCTCCAGGGGATGGCCGAAGCCCTCGTGGCCGTGGACCGCCAGGGA
CTGGTCATGAGCATCAACCGCACTGCCGCGGAACTTTTCGGGGTTGATGCGGCCGCGGTCCATGCCCGCCCCGTGGAGGA
ACTCCTCTCCGCCGCCATCCCGGCCGACCGGCTCCCCTGCCGCGTGGTGCTCGCCACGGGAGAGCCGTGCCTCGACGTGG
CCTACGACCTCCGCCTGCCGGGCCGGATTGTGCCGGTCCTCTCCAGTGCCGCCCCCCTGGCGAACGCCGCCGGCGAGATG
ATCGGGAGCGTGGAGATCATCCGCGACATCTCGGCCCTCAAGGCCCTGGAACAGGAGCGGGAGGATTTCGTCAGCATGCT
CTCCCACGACCTGAAGACCCCCATTACCGCCATCATCGGCTCCATCGACCTGGTGAAGGAGGGGCGGCTCGGTCCAGTGA
ATCCGGAACAGAGCATGTACCTGGACGCCGCCGTGGAGAGCTGCGCCGAGATGGTGGACATGATCGACACCCTGCTGGAT
GTGCACCGCTTCGAGGCGGGGCGGATGGTGACGACCCTTGTGGAAGAAGAGCTCGGGCCGCTCCTGCAGCGGACCGTTGG
CAGGTTCGAGCCGGTGGCCCGCCACAGCGGGCTCGAACTTACCCTCTCTCTACCCGGCGCTCCAGTCACCTTCCTGGTGG
ACCGGAAGCAATTCGGGCGACTCCTGGGCAACCTCCTCTCCAACGCCCTGAAGTTCACCACTGAGGGCGGCATCGAGGTA
CGGGCGGAGCTGCCGGAGAACGCCGCGGCGGCCGCACAGGGGATTACCCCCGGCGCCTACCCGACCGAGAGACTCCCCCG
GGAGGGACGATACCTGCTCGTTTCGGTGAGCGACACGGGGGCCGGGATTCCGGCCGATTCCCTCGTCACCATTTTCGACC
GCTTCGTCCAGGCCCGCAACCGCAAGATGGGGAAGTCGAGCGGCACCGGGCTCGGGCTCGCCTTCTGCCGCAAGGTCATG
GATGCCCACCACGGCTTCATCTGGGCCGAGAGCGAGCCTGACAGGGGGAGCACCTTCCGGATGCTTTTTCCGCTGGGGAC
CGACTGA

Upstream 100 bases:

>100_bases
AGGAAATCGTCGAACATCACCACGGCAGGATCTTCTACAACGAAGGGACCGGGGGGGGGAACGTTTTCACCTTCACGGTT
CCCGTCTATGAGGAACAATC

Downstream 100 bases:

>100_bases
CCACTGAGCGAGGGAACTTCGCCCATGAAACTGCCGTACCACACCATGCCTTTCAGGATCGCGGCGTACTATGCCCTCTG
TGCAGGGCTCTGGATCCTCC

Product: PAS/PAC sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNNLKILIVDDEADIALILKLQLEDAGYRTTRARDGLEALEMLAREHYDLILLDIRMPRLDGIEVLERIRQEWPELVVVM
MTAHGSEDIAVEAMKKGAVDYISKPFSSDDMKKRVERAIEYNRTRLENERLQREVEEERRKMEAILQGMAEALVAVDRQG
LVMSINRTAAELFGVDAAAVHARPVEELLSAAIPADRLPCRVVLATGEPCLDVAYDLRLPGRIVPVLSSAAPLANAAGEM
IGSVEIIRDISALKALEQEREDFVSMLSHDLKTPITAIIGSIDLVKEGRLGPVNPEQSMYLDAAVESCAEMVDMIDTLLD
VHRFEAGRMVTTLVEEELGPLLQRTVGRFEPVARHSGLELTLSLPGAPVTFLVDRKQFGRLLGNLLSNALKFTTEGGIEV
RAELPENAAAAAQGITPGAYPTERLPREGRYLLVSVSDTGAGIPADSLVTIFDRFVQARNRKMGKSSGTGLGLAFCRKVM
DAHHGFIWAESEPDRGSTFRMLFPLGTD

Sequences:

>Translated_508_residues
MNNLKILIVDDEADIALILKLQLEDAGYRTTRARDGLEALEMLAREHYDLILLDIRMPRLDGIEVLERIRQEWPELVVVM
MTAHGSEDIAVEAMKKGAVDYISKPFSSDDMKKRVERAIEYNRTRLENERLQREVEEERRKMEAILQGMAEALVAVDRQG
LVMSINRTAAELFGVDAAAVHARPVEELLSAAIPADRLPCRVVLATGEPCLDVAYDLRLPGRIVPVLSSAAPLANAAGEM
IGSVEIIRDISALKALEQEREDFVSMLSHDLKTPITAIIGSIDLVKEGRLGPVNPEQSMYLDAAVESCAEMVDMIDTLLD
VHRFEAGRMVTTLVEEELGPLLQRTVGRFEPVARHSGLELTLSLPGAPVTFLVDRKQFGRLLGNLLSNALKFTTEGGIEV
RAELPENAAAAAQGITPGAYPTERLPREGRYLLVSVSDTGAGIPADSLVTIFDRFVQARNRKMGKSSGTGLGLAFCRKVM
DAHHGFIWAESEPDRGSTFRMLFPLGTD
>Mature_508_residues
MNNLKILIVDDEADIALILKLQLEDAGYRTTRARDGLEALEMLAREHYDLILLDIRMPRLDGIEVLERIRQEWPELVVVM
MTAHGSEDIAVEAMKKGAVDYISKPFSSDDMKKRVERAIEYNRTRLENERLQREVEEERRKMEAILQGMAEALVAVDRQG
LVMSINRTAAELFGVDAAAVHARPVEELLSAAIPADRLPCRVVLATGEPCLDVAYDLRLPGRIVPVLSSAAPLANAAGEM
IGSVEIIRDISALKALEQEREDFVSMLSHDLKTPITAIIGSIDLVKEGRLGPVNPEQSMYLDAAVESCAEMVDMIDTLLD
VHRFEAGRMVTTLVEEELGPLLQRTVGRFEPVARHSGLELTLSLPGAPVTFLVDRKQFGRLLGNLLSNALKFTTEGGIEV
RAELPENAAAAAQGITPGAYPTERLPREGRYLLVSVSDTGAGIPADSLVTIFDRFVQARNRKMGKSSGTGLGLAFCRKVM
DAHHGFIWAESEPDRGSTFRMLFPLGTD

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=30.8300395256917, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788549, Length=394, Percent_Identity=25.3807106598985, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789149, Length=262, Percent_Identity=29.0076335877863, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=28.3524904214559, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=40.9448818897638, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788713, Length=395, Percent_Identity=23.2911392405063, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=33.8403041825095, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI48994928, Length=400, Percent_Identity=27.25, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790437, Length=123, Percent_Identity=38.2113821138211, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788550, Length=129, Percent_Identity=37.984496124031, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=29.0836653386454, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082128, Length=257, Percent_Identity=27.6264591439689, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1786600, Length=400, Percent_Identity=22.25, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788905, Length=115, Percent_Identity=34.7826086956522, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=253, Percent_Identity=28.4584980237154, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=36.8421052631579, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=30.9734513274336, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=31.6666666666667, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=112, Percent_Identity=33.9285714285714, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=35.6521739130435, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=31.0924369747899, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786784, Length=112, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=246, Percent_Identity=23.9837398373984, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790552, Length=126, Percent_Identity=30.952380952381, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55960; Mature: 55960

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLKILIVDDEADIALILKLQLEDAGYRTTRARDGLEALEMLAREHYDLILLDIRMPRL
CCCCEEEEEECCCCEEEEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCEEEEEEECCCC
DGIEVLERIRQEWPELVVVMMTAHGSEDIAVEAMKKGAVDYISKPFSSDDMKKRVERAIE
CCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
YNRTRLENERLQREVEEERRKMEAILQGMAEALVAVDRQGLVMSINRTAAELFGVDAAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHH
HARPVEELLSAAIPADRLPCRVVLATGEPCLDVAYDLRLPGRIVPVLSSAAPLANAAGEM
HHCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
IGSVEIIRDISALKALEQEREDFVSMLSHDLKTPITAIIGSIDLVKEGRLGPVNPEQSMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHCCCCCCCCCCCHHHH
LDAAVESCAEMVDMIDTLLDVHRFEAGRMVTTLVEEELGPLLQRTVGRFEPVARHSGLEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
TLSLPGAPVTFLVDRKQFGRLLGNLLSNALKFTTEGGIEVRAELPENAAAAAQGITPGAY
EEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCCC
PTERLPREGRYLLVSVSDTGAGIPADSLVTIFDRFVQARNRKMGKSSGTGLGLAFCRKVM
CHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DAHHGFIWAESEPDRGSTFRMLFPLGTD
HHHCCEEEECCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MNNLKILIVDDEADIALILKLQLEDAGYRTTRARDGLEALEMLAREHYDLILLDIRMPRL
CCCCEEEEEECCCCEEEEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCEEEEEEECCCC
DGIEVLERIRQEWPELVVVMMTAHGSEDIAVEAMKKGAVDYISKPFSSDDMKKRVERAIE
CCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
YNRTRLENERLQREVEEERRKMEAILQGMAEALVAVDRQGLVMSINRTAAELFGVDAAAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCHHHH
HARPVEELLSAAIPADRLPCRVVLATGEPCLDVAYDLRLPGRIVPVLSSAAPLANAAGEM
HHCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
IGSVEIIRDISALKALEQEREDFVSMLSHDLKTPITAIIGSIDLVKEGRLGPVNPEQSMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHCCCCCCCCCCCHHHH
LDAAVESCAEMVDMIDTLLDVHRFEAGRMVTTLVEEELGPLLQRTVGRFEPVARHSGLEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
TLSLPGAPVTFLVDRKQFGRLLGNLLSNALKFTTEGGIEVRAELPENAAAAAQGITPGAY
EEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCCC
PTERLPREGRYLLVSVSDTGAGIPADSLVTIFDRFVQARNRKMGKSSGTGLGLAFCRKVM
CHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
DAHHGFIWAESEPDRGSTFRMLFPLGTD
HHHCCEEEECCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA