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The map label for this gene is gyrB [H]

Identifier: 78221232

GI number: 78221232

Start: 4149

End: 6536

Strand: Direct

Name: gyrB [H]

Synonym: Gmet_0004

Alternate gene names: 78221232

Gene position: 4149-6536 (Clockwise)

Preceding gene: 78221231

Following gene: 78221233

Centisome position: 0.1

GC content: 61.47

Gene sequence:

>2388_bases
ATGGCTGACGAACTGAACAACGATTACGGAGCGGACAAGATCAAGGTCCTTGAGGGGCTCGAAGCTGTTCGCAAGCGCCC
CGCCATGTACATCGGCTCAACCGCGAGCCAGGGGCTCCACCATCTGGTCTACGAGATTGTCGATAACTCCATCGACGAGG
CCCTGGCCGGCTACTGCAACGAGGTGAACGTCACCATCCACCTGGACGGCTCCATTACGGTGGTGGACAACGGCCGGGGT
ATTCCCACCGAGATGCATCCCACTGAAGGGAAACCGGCGGCGGAGGTGGCCCTTACGGTTCTCCATGCCGGCGGTAAGTT
CGATAATACGTCATACAAGGTGTCCGGCGGTCTCCACGGGGTCGGCGTATCGGTCGTTAACGCCCTGTCGAAAAAGCTTG
AACTGGGAATTCGCCGTGACGGGAAGGTGTTTCAGCAGTCCTATGCCTACGGAGCACCGCTGACTCCCCTGGAAATTGTG
GGGGAGACCAAGAAACGGGGAACCAGAATCACTTTCCTCCCCGACGACACCATCTTCGAGACCACGGAATTTTCCTTCGA
TATTCTTTCCCAGCGGCTGCGGGAGCTGGCCTTCCTGAACGCCGGGGTGCGGATCACCATCTCCGACGAGCGGGAAGAGG
GTAAACACCACGACTTTCACTACGAGGGGGGAATCGTCTCCTTCGTGGAGTACCTGAACCGGAACCGAACCGTGCTCCAT
CCGAAACCGGTCTACTTCCGGGGTGAGAGGGCGGGGATCGACATGGAGATCGCCATCCAGTACAACGACTCCTACGACGA
GAAGGTTTTCACCTTTGCCAACAACATCAACACCCACGAGGGTGGTACCCACCTGGTGGGGTTCCGGGCGGCACTCACCC
GCTCCATGAACTCCTACGCCGCGGCCAACGAGCTGACGAAGAAGGAGAAGGTCTCCATCTCCGGCGAGGATCTGCGGGAG
GGGCTCACGGCGGTAATCTCAGTCAAGATTCCCCAGCCCCAGTTCGAGGGGCAGACCAAGACCAAGCTTGGGAACTCCGA
GGTGAAGGGGTTCGTGGAATCCCTCATGAACGAGCAGCTGGCGGTCTTCCTGGAGGAGAACCCGAAGATCGCCAAGGACA
TCATCGGCAAATCCATCGAAGCGGCCCGGGCCAGGGAAGCGGCCCGAAAGGCCCGGGAGCTGACCCGGCGTAAAAGTGCC
CTGGACATCTCCAGCCTCCCCGGCAAGCTGGCCGACTGCCAGGAGCGGGACCCGGCCCTCTGTGAGCTCTATCTCGTCGA
GGGTGACTCCGCCGGCGGCTCCGCCAAACAGGGACGGGACCGGAAGTTCCAGGCGATCCTGCCGCTCAAGGGGAAGATCC
TCAACGTGGAGAAAGCCCGGTTCGACAAGATGCTCTCCTCCCAGGAGATAGGCACCCTGATCACGGCCCTGGGTACCAGC
ATCGGCAAGGAAGACTTCGACGTGGCGAAGCTCCGCTACCACCGGATCATCGTCATGACCGACGCCGACGTGGACGGCTC
CCACATCCTGACGCTGCTTCTCACCTTCTTCTTCCGGCAGATGCCGGAGGTGGTGGAGCGGGGGCACCTCTACATCGCCC
AGCCGCCGCTCTACAAGGTGAAGCGGGGCCGCAAGGAGCTCTATCTCCGCAACGAGGCGGCCATGCAGGCTTACCTCCTG
GAGGAGGGGACCGAGGATATGGTCCTCTTCCTGGAGAATGGCGAGAAGACCTACACCGGCAAGCAGATCATCCCGATTCT
CAAGCAGCTGGTGGAGTACCGGACCATTCTGGACAAGGTGGTGCGCAAGGGGATCAATGAGGAGCTCATCCGGGTCTTTC
TGCGGCTCGGGGTCAAGGCCGGCATCGAGGACATGGAGCAGCTGGTGCCGTACCTGGCGAACATCTCCCGGGTCTACGAG
GGGGGTGATTCCAGCCCCCTGGACGACGGGCGGGCCATCGTGCGGCTCGGCAACCTCCGGATTGCCCTGGACCAGCACAC
TCTCGACATCATCGGATCCTACGAGTACGGGCTCCTGGCCGAAAGCTTCCGGAAGGTCAGGGAGATGTTCGGCGACGGCC
GGGCCGTGGTCTCCAGCGAGGGGAAGGAGCTCTTCGCCACCGCCATCGGGCTCGACCTCCTCTCCTTCTTCATGGACTCC
GCCAAGAAGGGGCTCTCCATCCAGCGCTATAAAGGTCTCGGCGAGATGAACCCGGACCAGCTCTGGGAGACGACCATGGA
GCCCACCAACCGGACGCTCCTCCAGGTGAAGATCGAGGACGCCATCGAGGCCGACACCATCTTTACCATCCTCATGGGAG
ACCAGGTGGAGCCCCGCCGGGACTTCATCGAGAAGAACGCCCTGAACGTGTCGAACCTCGACATCTGA

Upstream 100 bases:

>100_bases
ACCACCACCGATGTTTCAACCCTCCGCCTGGCGGGGATTGCAACCCACGCGTCCTTCCACGTTTCACGTGGAACGGTGAC
GCCTTTATAGAGGGAGAAGT

Downstream 100 bases:

>100_bases
TGACCGATATTAGTAATTATCCGAGGAAACAGAATGCTTGAACAGACCCTGAACAAGACTGCCGTCAATATCGAAGACGA
GATGAAACGCTCGTACATGG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 795; Mature: 794

Protein sequence:

>795_residues
MADELNNDYGADKIKVLEGLEAVRKRPAMYIGSTASQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSITVVDNGRG
IPTEMHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSKKLELGIRRDGKVFQQSYAYGAPLTPLEIV
GETKKRGTRITFLPDDTIFETTEFSFDILSQRLRELAFLNAGVRITISDEREEGKHHDFHYEGGIVSFVEYLNRNRTVLH
PKPVYFRGERAGIDMEIAIQYNDSYDEKVFTFANNINTHEGGTHLVGFRAALTRSMNSYAAANELTKKEKVSISGEDLRE
GLTAVISVKIPQPQFEGQTKTKLGNSEVKGFVESLMNEQLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKSA
LDISSLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTS
IGKEDFDVAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLL
EEGTEDMVLFLENGEKTYTGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKAGIEDMEQLVPYLANISRVYE
GGDSSPLDDGRAIVRLGNLRIALDQHTLDIIGSYEYGLLAESFRKVREMFGDGRAVVSSEGKELFATAIGLDLLSFFMDS
AKKGLSIQRYKGLGEMNPDQLWETTMEPTNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEKNALNVSNLDI

Sequences:

>Translated_795_residues
MADELNNDYGADKIKVLEGLEAVRKRPAMYIGSTASQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSITVVDNGRG
IPTEMHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSKKLELGIRRDGKVFQQSYAYGAPLTPLEIV
GETKKRGTRITFLPDDTIFETTEFSFDILSQRLRELAFLNAGVRITISDEREEGKHHDFHYEGGIVSFVEYLNRNRTVLH
PKPVYFRGERAGIDMEIAIQYNDSYDEKVFTFANNINTHEGGTHLVGFRAALTRSMNSYAAANELTKKEKVSISGEDLRE
GLTAVISVKIPQPQFEGQTKTKLGNSEVKGFVESLMNEQLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKSA
LDISSLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTS
IGKEDFDVAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLL
EEGTEDMVLFLENGEKTYTGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKAGIEDMEQLVPYLANISRVYE
GGDSSPLDDGRAIVRLGNLRIALDQHTLDIIGSYEYGLLAESFRKVREMFGDGRAVVSSEGKELFATAIGLDLLSFFMDS
AKKGLSIQRYKGLGEMNPDQLWETTMEPTNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEKNALNVSNLDI
>Mature_794_residues
ADELNNDYGADKIKVLEGLEAVRKRPAMYIGSTASQGLHHLVYEIVDNSIDEALAGYCNEVNVTIHLDGSITVVDNGRGI
PTEMHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHGVGVSVVNALSKKLELGIRRDGKVFQQSYAYGAPLTPLEIVG
ETKKRGTRITFLPDDTIFETTEFSFDILSQRLRELAFLNAGVRITISDEREEGKHHDFHYEGGIVSFVEYLNRNRTVLHP
KPVYFRGERAGIDMEIAIQYNDSYDEKVFTFANNINTHEGGTHLVGFRAALTRSMNSYAAANELTKKEKVSISGEDLREG
LTAVISVKIPQPQFEGQTKTKLGNSEVKGFVESLMNEQLAVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKSAL
DISSLPGKLADCQERDPALCELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTSI
GKEDFDVAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKVKRGRKELYLRNEAAMQAYLLE
EGTEDMVLFLENGEKTYTGKQIIPILKQLVEYRTILDKVVRKGINEELIRVFLRLGVKAGIEDMEQLVPYLANISRVYEG
GDSSPLDDGRAIVRLGNLRIALDQHTLDIIGSYEYGLLAESFRKVREMFGDGRAVVSSEGKELFATAIGLDLLSFFMDSA
KKGLSIQRYKGLGEMNPDQLWETTMEPTNRTLLQVKIEDAIEADTIFTILMGDQVEPRRDFIEKNALNVSNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=581, Percent_Identity=25.9896729776248, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=25.3781512605042, Blast_Score=126, Evalue=7e-29,
Organism=Escherichia coli, GI48994957, Length=811, Percent_Identity=54.6239210850801, Blast_Score=827, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=559, Percent_Identity=40.2504472271914, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI212645845, Length=536, Percent_Identity=27.0522388059701, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17535065, Length=535, Percent_Identity=26.3551401869159, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=222, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=583, Percent_Identity=27.7873070325901, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17136538, Length=574, Percent_Identity=26.8292682926829, Blast_Score=142, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88598; Mature: 88467

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADELNNDYGADKIKVLEGLEAVRKRPAMYIGSTASQGLHHLVYEIVDNSIDEALAGYCN
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCC
EVNVTIHLDGSITVVDNGRGIPTEMHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHG
CEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCEEECCCCCH
VGVSVVNALSKKLELGIRRDGKVFQQSYAYGAPLTPLEIVGETKKRGTRITFLPDDTIFE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEECCCCCEEC
TTEFSFDILSQRLRELAFLNAGVRITISDEREEGKHHDFHYEGGIVSFVEYLNRNRTVLH
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEECCCHHHHHHHHCCCCEEEC
PKPVYFRGERAGIDMEIAIQYNDSYDEKVFTFANNINTHEGGTHLVGFRAALTRSMNSYA
CCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHH
AANELTKKEKVSISGEDLREGLTAVISVKIPQPQFEGQTKTKLGNSEVKGFVESLMNEQL
HHHHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHCCCE
AVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKSALDISSLPGKLADCQERDPAL
EEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCE
CELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTS
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
IGKEDFDVAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKV
CCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRGRKELYLRNEAAMQAYLLEEGTEDMVLFLENGEKTYTGKQIIPILKQLVEYRTILDKV
HCCCHHEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VRKGINEELIRVFLRLGVKAGIEDMEQLVPYLANISRVYEGGDSSPLDDGRAIVRLGNLR
HHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEE
IALDQHTLDIIGSYEYGLLAESFRKVREMFGDGRAVVSSEGKELFATAIGLDLLSFFMDS
EEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AKKGLSIQRYKGLGEMNPDQLWETTMEPTNRTLLQVKIEDAIEADTIFTILMGDQVEPRR
HHCCCEEHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCHHH
DFIEKNALNVSNLDI
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADELNNDYGADKIKVLEGLEAVRKRPAMYIGSTASQGLHHLVYEIVDNSIDEALAGYCN
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCC
EVNVTIHLDGSITVVDNGRGIPTEMHPTEGKPAAEVALTVLHAGGKFDNTSYKVSGGLHG
CEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCEEECCCCCH
VGVSVVNALSKKLELGIRRDGKVFQQSYAYGAPLTPLEIVGETKKRGTRITFLPDDTIFE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHCCHHHCCCEEEECCCCCEEC
TTEFSFDILSQRLRELAFLNAGVRITISDEREEGKHHDFHYEGGIVSFVEYLNRNRTVLH
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEECCCHHHHHHHHCCCCEEEC
PKPVYFRGERAGIDMEIAIQYNDSYDEKVFTFANNINTHEGGTHLVGFRAALTRSMNSYA
CCCCEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHH
AANELTKKEKVSISGEDLREGLTAVISVKIPQPQFEGQTKTKLGNSEVKGFVESLMNEQL
HHHHHHHHHHCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHCCCE
AVFLEENPKIAKDIIGKSIEAARAREAARKARELTRRKSALDISSLPGKLADCQERDPAL
EEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCE
CELYLVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSQEIGTLITALGTS
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHH
IGKEDFDVAKLRYHRIIVMTDADVDGSHILTLLLTFFFRQMPEVVERGHLYIAQPPLYKV
CCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
KRGRKELYLRNEAAMQAYLLEEGTEDMVLFLENGEKTYTGKQIIPILKQLVEYRTILDKV
HCCCHHEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
VRKGINEELIRVFLRLGVKAGIEDMEQLVPYLANISRVYEGGDSSPLDDGRAIVRLGNLR
HHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEE
IALDQHTLDIIGSYEYGLLAESFRKVREMFGDGRAVVSSEGKELFATAIGLDLLSFFMDS
EEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AKKGLSIQRYKGLGEMNPDQLWETTMEPTNRTLLQVKIEDAIEADTIFTILMGDQVEPRR
HHCCCEEHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCHHH
DFIEKNALNVSNLDI
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]