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The map label for this gene is clpB1 [H]

Identifier: 78189926

GI number: 78189926

Start: 2505100

End: 2506419

Strand: Reverse

Name: clpB1 [H]

Synonym: Cag_1973

Alternate gene names: 78189926

Gene position: 2506419-2505100 (Counterclockwise)

Preceding gene: 78189931

Following gene: 78189925

Centisome position: 97.45

GC content: 48.86

Gene sequence:

>1320_bases
ATGAGTACTACTGATACCCGCCAACGGTTGGAGGAAATAGAGAAGCAGTTAGCAAGTTTAACCGAAGAGCGCAATACTAT
TCGGGCGCGTTGGGAAAGCGAAAAGGAGCTGCTGCAACTTTCGCGCGACTTGCAATCGCAAATTGAGCAGTTGCGTGTGC
AGGCTGAGGAGTTTGAACGGCAAGGCGATTATGGCAAGGTTGCCGAAATTCGCTATGGCAAAATTGCTCAAATTGAGCGC
GACCTTGCGGAGAATCGTAAAAAAATTGAGGAGAAAAAGTTGGGTGGCAATCTCATTATGAAGGAGGAGATTGATGCTGA
GGATATTGCCGATATTGTGTCGCGCTGGACGGGCATTCCGTTAAGCAAAATGTTGCAAACGGAGCGCCAAAAGTTGCTCT
TAATTGAGGATGAGCTGCATAAGCGGGTGATTGGGCAGCATGAAGCCGTTACTGCCGTAAGCGAAGCGGTCAAGCGCTCG
CGTGCGGGCATGGGCGATGAAAAGCGTCCGATTGGCTCCTTTATTTTTCTTGGACCAACAGGTGTGGGTAAAACGGAGCT
GGCGCGCACCCTTGCCGATTATCTTTTTGATGACGAAGATGCAATGATTCGCATTGATATGAGCGAATACATGGAGTCGC
ATAACGTGAGCCGCTTGGTGGGAGCTCCTCCCGGCTATGTTGGTTATGAAGAGGGTGGGCAGCTTACCGAAGCGGTACGC
CGCAAGCCATTTTCCGTTGTGCTGCTTGATGAAATTGAAAAAGCGCATCCCGACGTGTTTAATATTCTCTTACAAATTCT
TGATGATGGGCGCTTAACCGATAGCAAAGGGCGCACGGTGAACTTTAAGAATTGCATCATTATTATGACGAGCAATATTG
GTGCCCAGCTTATTCAGTCGGAATTGGAGCAGCTTGAAGGAGCTAAGCGCGACGAGGTGCTTGCGGGCTTGCAGGAGAAG
CTCTTTCAATTGTTGAAGCAAAAGGTTCGTCCTGAATTTTTGAACCGTATTGATGAGGTGATTCTCTTTACCCCGCTGAC
GCGCGACGACTTGCAAAAAATTGTGCTCATTCAGTTTGAGCATATTCAAGAGGCGGCGCTTCGTCAACATATCACCCTTA
CCATTTCGGATGAGGCAATTGTATGGCTTGCAAAAACGGGCTTTGATCCCGCATTTGGTGCGCGTCCGCTCAAGCGTGTT
ATGCAGCGTAAAATTACCAATAAGCTCTCTGAGTTGATTTTATCGGGAGCCGTACAAGAGGGCGAAAGCGTTGCGGTGGA
GTGCGTTGGCGACGAATTAGTGGTTCGCAAAAACGCATGA

Upstream 100 bases:

>100_bases
GCCTCTATTAATGTACAACAATATTTTTACATGAATGGCACCATAGCGTTTTCTGCCGCAGCGGTGCATTTCTCTCTAAA
AAAATAAAACCATAAACACC

Downstream 100 bases:

>100_bases
GACGGCGTTTGCGGATGGTTGCGTTGGTGCTGCTATGGCTTTGCATGGCAGCACCTCTCGCTATGGCGGCAGCTTCGCCC
GATAGCCTTTCCATAAAAAT

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSTTDTRQRLEEIEKQLASLTEERNTIRARWESEKELLQLSRDLQSQIEQLRVQAEEFERQGDYGKVAEIRYGKIAQIER
DLAENRKKIEEKKLGGNLIMKEEIDAEDIADIVSRWTGIPLSKMLQTERQKLLLIEDELHKRVIGQHEAVTAVSEAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLADYLFDDEDAMIRIDMSEYMESHNVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNCIIIMTSNIGAQLIQSELEQLEGAKRDEVLAGLQEK
LFQLLKQKVRPEFLNRIDEVILFTPLTRDDLQKIVLIQFEHIQEAALRQHITLTISDEAIVWLAKTGFDPAFGARPLKRV
MQRKITNKLSELILSGAVQEGESVAVECVGDELVVRKNA

Sequences:

>Translated_439_residues
MSTTDTRQRLEEIEKQLASLTEERNTIRARWESEKELLQLSRDLQSQIEQLRVQAEEFERQGDYGKVAEIRYGKIAQIER
DLAENRKKIEEKKLGGNLIMKEEIDAEDIADIVSRWTGIPLSKMLQTERQKLLLIEDELHKRVIGQHEAVTAVSEAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLADYLFDDEDAMIRIDMSEYMESHNVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNCIIIMTSNIGAQLIQSELEQLEGAKRDEVLAGLQEK
LFQLLKQKVRPEFLNRIDEVILFTPLTRDDLQKIVLIQFEHIQEAALRQHITLTISDEAIVWLAKTGFDPAFGARPLKRV
MQRKITNKLSELILSGAVQEGESVAVECVGDELVVRKNA
>Mature_438_residues
STTDTRQRLEEIEKQLASLTEERNTIRARWESEKELLQLSRDLQSQIEQLRVQAEEFERQGDYGKVAEIRYGKIAQIERD
LAENRKKIEEKKLGGNLIMKEEIDAEDIADIVSRWTGIPLSKMLQTERQKLLLIEDELHKRVIGQHEAVTAVSEAVKRSR
AGMGDEKRPIGSFIFLGPTGVGKTELARTLADYLFDDEDAMIRIDMSEYMESHNVSRLVGAPPGYVGYEEGGQLTEAVRR
KPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNCIIIMTSNIGAQLIQSELEQLEGAKRDEVLAGLQEKL
FQLLKQKVRPEFLNRIDEVILFTPLTRDDLQKIVLIQFEHIQEAALRQHITLTISDEAIVWLAKTGFDPAFGARPLKRVM
QRKITNKLSELILSGAVQEGESVAVECVGDELVVRKNA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=38.255033557047, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1788943, Length=430, Percent_Identity=49.5348837209302, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787109, Length=326, Percent_Identity=42.9447852760736, Blast_Score=273, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=431, Percent_Identity=43.1554524361949, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323002, Length=429, Percent_Identity=40.5594405594406, Blast_Score=329, Evalue=5e-91,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49843; Mature: 49712

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDTRQRLEEIEKQLASLTEERNTIRARWESEKELLQLSRDLQSQIEQLRVQAEEFER
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDYGKVAEIRYGKIAQIERDLAENRKKIEEKKLGGNLIMKEEIDAEDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCC
LSKMLQTERQKLLLIEDELHKRVIGQHEAVTAVSEAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLADYLFDDEDAMIRIDMSEYMESHNVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNCIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
ELEQLEGAKRDEVLAGLQEKLFQLLKQKVRPEFLNRIDEVILFTPLTRDDLQKIVLIQFE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
HIQEAALRQHITLTISDEAIVWLAKTGFDPAFGARPLKRVMQRKITNKLSELILSGAVQE
HHHHHHHHHCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GESVAVECVGDELVVRKNA
CCCEEEEEECCEEEEECCC
>Mature Secondary Structure 
STTDTRQRLEEIEKQLASLTEERNTIRARWESEKELLQLSRDLQSQIEQLRVQAEEFER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGDYGKVAEIRYGKIAQIERDLAENRKKIEEKKLGGNLIMKEEIDAEDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCC
LSKMLQTERQKLLLIEDELHKRVIGQHEAVTAVSEAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLADYLFDDEDAMIRIDMSEYMESHNVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNCIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
ELEQLEGAKRDEVLAGLQEKLFQLLKQKVRPEFLNRIDEVILFTPLTRDDLQKIVLIQFE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
HIQEAALRQHITLTISDEAIVWLAKTGFDPAFGARPLKRVMQRKITNKLSELILSGAVQE
HHHHHHHHHCEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GESVAVECVGDELVVRKNA
CCCEEEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]