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The map label for this gene is cobS

Identifier: 78189024

GI number: 78189024

Start: 1438222

End: 1438980

Strand: Reverse

Name: cobS

Synonym: Cag_1057

Alternate gene names: 78189024

Gene position: 1438980-1438222 (Counterclockwise)

Preceding gene: 78189025

Following gene: 78189023

Centisome position: 55.95

GC content: 51.25

Gene sequence:

>759_bases
ATGCTTGGTGGCTTAGTAACGGCGCTTCGTACTTTAACAATTTTGCCGATACCGGGGAAGGATGCTGTTACCTTTAGTCA
TTCATTGTACTGGTTTCCGTTTGTTGGATTGTTGCTTGGTGCTCTGCTTGCGGCGCTTGGCTACGTTGGTTCGCTGAGTG
GCTGGCATGAATTTGCCGCCCTCTTGGTGGTGCTTGGTGGCATTGTGCTTACACGAGGTATGCACGCCGACGGTTTAGCT
GATGTTGCCGATGGTTTTTGGGGCGGTCGTTCAAAAGAGGCTGCCCTTCGTATTATGAAGGATCCCACGGTTGGCTCGTT
TGGAGCGTTGGCACTTAGCGGAGTAATGCTTTTAAAATGGGTTGCTGTGGTGCGCTTGCTTTCGTTTGGTTTGTTTGATG
TGGTGATGGCTGGCATCTTGCTTGCTCGTTTGGTACAAGTGCTGTTGGCATCGGCGCTGCCTTACGCTCGGCGCGAAGCT
GGCACCGCAAGTGCATTTGTGGCAGGTGCAGGTGCTCCTCATGCCTTTTCGGCGTTACTCTTTACTTTGGCGCTCCTCTT
TCCTTTTTATACAGAGAATTTCCCTACCATGCTGTGGCTTCTTGGTGCAGCACTTGTTGCGGGTAGCATGGTTGGGATGG
TAAGCTACCGCAAAATTGGTGGTGTTACGGGTGATGTGCTTGGTGCAGGCAGCGAACTGACGGAAGTTGCTGTTTGGATT
ACGGGTGCGTTACTTCTCTCCGATTATCTGCTTTTTTGA

Upstream 100 bases:

>100_bases
AGCGCTTGCTATGCAGTTAGTGGATGCCGCGCTTAAAATTTATAATGAAATGGCAACCTTTAGTGGTGCTCAAGTGAGTG
AAAAAATTGAAGGATAGGTT

Downstream 100 bases:

>100_bases
TCTCACGCTTATTTCCTGCCTTACACAATTTCTTCTTTAAAAGAATATTCGGAATACAAAACGGTTTTTTTTTTATTTTG
CTTTGAAAAGAAAAGCTAAA

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLGGLVTALRTLTILPIPGKDAVTFSHSLYWFPFVGLLLGALLAALGYVGSLSGWHEFAALLVVLGGIVLTRGMHADGLA
DVADGFWGGRSKEAALRIMKDPTVGSFGALALSGVMLLKWVAVVRLLSFGLFDVVMAGILLARLVQVLLASALPYARREA
GTASAFVAGAGAPHAFSALLFTLALLFPFYTENFPTMLWLLGAALVAGSMVGMVSYRKIGGVTGDVLGAGSELTEVAVWI
TGALLLSDYLLF

Sequences:

>Translated_252_residues
MLGGLVTALRTLTILPIPGKDAVTFSHSLYWFPFVGLLLGALLAALGYVGSLSGWHEFAALLVVLGGIVLTRGMHADGLA
DVADGFWGGRSKEAALRIMKDPTVGSFGALALSGVMLLKWVAVVRLLSFGLFDVVMAGILLARLVQVLLASALPYARREA
GTASAFVAGAGAPHAFSALLFTLALLFPFYTENFPTMLWLLGAALVAGSMVGMVSYRKIGGVTGDVLGAGSELTEVAVWI
TGALLLSDYLLF
>Mature_252_residues
MLGGLVTALRTLTILPIPGKDAVTFSHSLYWFPFVGLLLGALLAALGYVGSLSGWHEFAALLVVLGGIVLTRGMHADGLA
DVADGFWGGRSKEAALRIMKDPTVGSFGALALSGVMLLKWVAVVRLLSFGLFDVVMAGILLARLVQVLLASALPYARREA
GTASAFVAGAGAPHAFSALLFTLALLFPFYTENFPTMLWLLGAALVAGSMVGMVSYRKIGGVTGDVLGAGSELTEVAVWI
TGALLLSDYLLF

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=236, Percent_Identity=33.8983050847458, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_CHLCH (Q3ARQ6)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379362.1
- STRING:   Q3ARQ6
- GeneID:   3747040
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1057
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   RIMKQSD
- ProtClustDB:   CLSK637530
- BioCyc:   CCHL340177:CAG_1057-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 26318; Mature: 26318

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10eae7b0)-; HASH(0x10b9c810)-; HASH(0x10e9ed00)-; HASH(0x44a20230)-; HASH(0x10dbc608)-; HASH(0x114df518)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGGLVTALRTLTILPIPGKDAVTFSHSLYWFPFVGLLLGALLAALGYVGSLSGWHEFAA
CCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLVVLGGIVLTRGMHADGLADVADGFWGGRSKEAALRIMKDPTVGSFGALALSGVMLLKW
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VAVVRLLSFGLFDVVMAGILLARLVQVLLASALPYARREAGTASAFVAGAGAPHAFSALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHH
FTLALLFPFYTENFPTMLWLLGAALVAGSMVGMVSYRKIGGVTGDVLGAGSELTEVAVWI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
TGALLLSDYLLF
HHHHHHHHHHCH
>Mature Secondary Structure
MLGGLVTALRTLTILPIPGKDAVTFSHSLYWFPFVGLLLGALLAALGYVGSLSGWHEFAA
CCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LLVVLGGIVLTRGMHADGLADVADGFWGGRSKEAALRIMKDPTVGSFGALALSGVMLLKW
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
VAVVRLLSFGLFDVVMAGILLARLVQVLLASALPYARREAGTASAFVAGAGAPHAFSALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHH
FTLALLFPFYTENFPTMLWLLGAALVAGSMVGMVSYRKIGGVTGDVLGAGSELTEVAVWI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHH
TGALLLSDYLLF
HHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA