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The map label for this gene is topA [H]

Identifier: 78188059

GI number: 78188059

Start: 83182

End: 85578

Strand: Reverse

Name: topA [H]

Synonym: Cag_0075

Alternate gene names: 78188059

Gene position: 85578-83182 (Counterclockwise)

Preceding gene: 78188068

Following gene: 78188056

Centisome position: 3.33

GC content: 47.93

Gene sequence:

>2397_bases
ATGGCGTCATCAGTAGCAGCACTCTCTGCCAAAAATAAAACCCTCATTGTGGTTGAGTCGCCGTCAAAGGCAAAAACCAT
TAACAAGTATCTCGGCAGCAACTATACGGTTTTCGCCTCCGTCGGGCACATTAAAGATTTACCGAAAAAAGAGATTGGGC
TCGACTTTGAACATAACTACTCCCCTCGTTACGAAATTATTCCCGGTAAAGAAAAGGTAGTAAAGCAGCTTAAAAAACTT
GCGACTGAAGCCAGCAACATTTTGATTGCTACTGACCCTGACCGCGAGGGTGAAGCTATTGCATGGCACATTGCGAACGA
AATAGAACATGCCAAAGCACCCGTTGCCCGCGTGCTCTTTAACGAAGTTACCAAAAAAGCCATTCTTGAGGCAATTGAAA
AACCTCGCCATATTGACTTGCGCCTTGTGCATTCCCAGCAAACAAGGCAGGGGCTCGATAAAATTGTAGGTTATAAAATC
AGCCCATTTTTGTGGAAAGTGGTGCTGCGTGGATTATCGGCAGGGCGCGTACAATCGGTGGCGTTACGCCTTATTTGTGA
GCGTGAAGAGGAGATTGAGCGCTTTGTTATTCAAGAATATTGGACTATTGCTGCCGATTTTCTAACGGCAAACAAAGAAT
CCTTCCGCGCCCGCTTAGTGCGTTTGGATGGCGATAAACCTGAAATCACGAATGTAGAGCAAGCAGAAGCTATTGCCGCT
ATTGCCAAAAAAGGCAACTACAGCGTTCGCGAAATTACTCCTCGCATTCAACAACGAAAACAGCCGCTACCGTTTACGAC
ATCGTTGTTGCAGCAAGCCGCCTCTAACCAGCTTGGTTTTGGTGCACAGCGCACCATGCGCACCGCCCAGCAACTTTACG
AAGGTATTGAACTTGGTGCTGAAGGTGCTATGGGTCTTATTACCTACATGCGTACCGACTCCACACGCATTAGCCCCGAA
GCAGTTGGCGAAGCACGCAACTACATTGAGCGCAATTTTGGCAAAGATTACGTTGGAGCTGGTAGCAGCGGCAAACCCGG
CAAAAATGCTCAAGATGCCCACGAAGCCATTCGCCCCACCTCGCTCCTTAAAACGCCTGAACAGGTAAAACCCTACCTCT
CCGCCGATCAATTCAAGCTCTATGAATTAATTTGGAAGCGCTTTCTTGCTGCAATGATGGCTCCCGCTAAAATTGAGCAA
ACAAAGGTTGATGTTGAGGAGCAAAGCGGCAAATTTCTTTTTCGAGCAAATGGAAGCCGCGTACTCTTCCCTGGCTTTAT
GCGCGTTTATGACGATCAGCAAGAGCTTGCATATGAGGCACAAACCTCCACAAAAGAAGAGGTGGAAAATGAAATGGTGG
TAAAACTTCCAGAAAAACTTGCCGTTAATGACCCGCTTGGGCTTGGCGCATTAGAACAAAAACAAAGCTTTACCCGTCCA
CCTGCTCGTTACAGCGAAGCAAGCTTAGTAAAAGACCTCGACCACTTTGGCATTGGGCGCCCATCAACCTACGCCTCTAT
TTTTTCAACTTTGCAAGATCGCCGCTATGTGGCACTTGAAAAGCGCAAAATTATGCCCACCGATCTTGGGCGTGATGTTG
CTAAAATTCTTGTAGCCAACTTCCCCGAACTCTTTAACGTTGGCTTTACCGCCTTTATGGAGGACGAGCTTGATAAAGTT
GCAAGTGGCGACGATGCGTATGAAAAAGTGCTCGATAGCTTTTACAAGCCTCTCACATCAGCTTTAGCGCTTCGCAGTGC
CACGCCACTCATTCCGCAAAACAACGAAGCCGAAACATGCGACAAATGCGGCACAGGCAAAATGATTTTAAAATGGACAG
CAAGCGGCAAATTTCTTGGATGCTCCAACTATCCCAAGTGCAAAAACATTCGCACCATTAGTAGCAATCGCGAAAAACCC
GCATCAACGGGCGTGCACTGCCCCTCATGCGAAGATGGTGAAATGGTGCTCCGCAAAGGACGACTTGGACCATTTCTTGC
CTGCTCCAACTATCCCAAATGCAATACGCTGCTTAACCTAAACAAGCAACGCCACATTGAGCCACCGAAAACACCACCCG
TTGTTACCGATATGGCGTGCCCAAAATGCGGTGCCCCACTTTACCTCCGCAGTGGCAAACGAGGGCTATGGCTTGGCTGC
TCAAAATTCCCCAAATGTAGAGGACGCCTTGCATGGACAGCGCTTGAGCCTGCTGCTCAAGAGCGATGGGAACGCGTTAT
GGCTGCACATCAAAAAGCACATCCCCCCGTTACCTTAAAAATGGTTGATGGTAGTACTGTTTCAATGACCAGCTCAATTG
ATGATATTATTATGAAAGCTGACGCCGCAGGATTAATTGCTCCCGCAATGGATTTAGTGCCTGAAGCGGAGGGATAG

Upstream 100 bases:

>100_bases
TTTGAATTGATATTGTAATAATAGAGGCACGGCATCACAAGCTATCCCTACAAATGCCGCCTCACTCCACTAATCTACCT
ACCCTTCGGGTTCCAACGCC

Downstream 100 bases:

>100_bases
AAGGGCGTGGAGGCACTTGTTATGCAGGAAAAATTCGATCAGGAATTGCTTAATAAACACAAACAAGCTTTTTTAAAGGA
AAATTGCGACGAAACATTAC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 798; Mature: 797

Protein sequence:

>798_residues
MASSVAALSAKNKTLIVVESPSKAKTINKYLGSNYTVFASVGHIKDLPKKEIGLDFEHNYSPRYEIIPGKEKVVKQLKKL
ATEASNILIATDPDREGEAIAWHIANEIEHAKAPVARVLFNEVTKKAILEAIEKPRHIDLRLVHSQQTRQGLDKIVGYKI
SPFLWKVVLRGLSAGRVQSVALRLICEREEEIERFVIQEYWTIAADFLTANKESFRARLVRLDGDKPEITNVEQAEAIAA
IAKKGNYSVREITPRIQQRKQPLPFTTSLLQQAASNQLGFGAQRTMRTAQQLYEGIELGAEGAMGLITYMRTDSTRISPE
AVGEARNYIERNFGKDYVGAGSSGKPGKNAQDAHEAIRPTSLLKTPEQVKPYLSADQFKLYELIWKRFLAAMMAPAKIEQ
TKVDVEEQSGKFLFRANGSRVLFPGFMRVYDDQQELAYEAQTSTKEEVENEMVVKLPEKLAVNDPLGLGALEQKQSFTRP
PARYSEASLVKDLDHFGIGRPSTYASIFSTLQDRRYVALEKRKIMPTDLGRDVAKILVANFPELFNVGFTAFMEDELDKV
ASGDDAYEKVLDSFYKPLTSALALRSATPLIPQNNEAETCDKCGTGKMILKWTASGKFLGCSNYPKCKNIRTISSNREKP
ASTGVHCPSCEDGEMVLRKGRLGPFLACSNYPKCNTLLNLNKQRHIEPPKTPPVVTDMACPKCGAPLYLRSGKRGLWLGC
SKFPKCRGRLAWTALEPAAQERWERVMAAHQKAHPPVTLKMVDGSTVSMTSSIDDIIMKADAAGLIAPAMDLVPEAEG

Sequences:

>Translated_798_residues
MASSVAALSAKNKTLIVVESPSKAKTINKYLGSNYTVFASVGHIKDLPKKEIGLDFEHNYSPRYEIIPGKEKVVKQLKKL
ATEASNILIATDPDREGEAIAWHIANEIEHAKAPVARVLFNEVTKKAILEAIEKPRHIDLRLVHSQQTRQGLDKIVGYKI
SPFLWKVVLRGLSAGRVQSVALRLICEREEEIERFVIQEYWTIAADFLTANKESFRARLVRLDGDKPEITNVEQAEAIAA
IAKKGNYSVREITPRIQQRKQPLPFTTSLLQQAASNQLGFGAQRTMRTAQQLYEGIELGAEGAMGLITYMRTDSTRISPE
AVGEARNYIERNFGKDYVGAGSSGKPGKNAQDAHEAIRPTSLLKTPEQVKPYLSADQFKLYELIWKRFLAAMMAPAKIEQ
TKVDVEEQSGKFLFRANGSRVLFPGFMRVYDDQQELAYEAQTSTKEEVENEMVVKLPEKLAVNDPLGLGALEQKQSFTRP
PARYSEASLVKDLDHFGIGRPSTYASIFSTLQDRRYVALEKRKIMPTDLGRDVAKILVANFPELFNVGFTAFMEDELDKV
ASGDDAYEKVLDSFYKPLTSALALRSATPLIPQNNEAETCDKCGTGKMILKWTASGKFLGCSNYPKCKNIRTISSNREKP
ASTGVHCPSCEDGEMVLRKGRLGPFLACSNYPKCNTLLNLNKQRHIEPPKTPPVVTDMACPKCGAPLYLRSGKRGLWLGC
SKFPKCRGRLAWTALEPAAQERWERVMAAHQKAHPPVTLKMVDGSTVSMTSSIDDIIMKADAAGLIAPAMDLVPEAEG
>Mature_797_residues
ASSVAALSAKNKTLIVVESPSKAKTINKYLGSNYTVFASVGHIKDLPKKEIGLDFEHNYSPRYEIIPGKEKVVKQLKKLA
TEASNILIATDPDREGEAIAWHIANEIEHAKAPVARVLFNEVTKKAILEAIEKPRHIDLRLVHSQQTRQGLDKIVGYKIS
PFLWKVVLRGLSAGRVQSVALRLICEREEEIERFVIQEYWTIAADFLTANKESFRARLVRLDGDKPEITNVEQAEAIAAI
AKKGNYSVREITPRIQQRKQPLPFTTSLLQQAASNQLGFGAQRTMRTAQQLYEGIELGAEGAMGLITYMRTDSTRISPEA
VGEARNYIERNFGKDYVGAGSSGKPGKNAQDAHEAIRPTSLLKTPEQVKPYLSADQFKLYELIWKRFLAAMMAPAKIEQT
KVDVEEQSGKFLFRANGSRVLFPGFMRVYDDQQELAYEAQTSTKEEVENEMVVKLPEKLAVNDPLGLGALEQKQSFTRPP
ARYSEASLVKDLDHFGIGRPSTYASIFSTLQDRRYVALEKRKIMPTDLGRDVAKILVANFPELFNVGFTAFMEDELDKVA
SGDDAYEKVLDSFYKPLTSALALRSATPLIPQNNEAETCDKCGTGKMILKWTASGKFLGCSNYPKCKNIRTISSNREKPA
STGVHCPSCEDGEMVLRKGRLGPFLACSNYPKCNTLLNLNKQRHIEPPKTPPVVTDMACPKCGAPLYLRSGKRGLWLGCS
KFPKCRGRLAWTALEPAAQERWERVMAAHQKAHPPVTLKMVDGSTVSMTSSIDDIIMKADAAGLIAPAMDLVPEAEG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=719, Percent_Identity=25.3129346314325, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1787529, Length=820, Percent_Identity=39.6341463414634, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1788061, Length=642, Percent_Identity=24.9221183800623, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI48994931, Length=140, Percent_Identity=32.8571428571429, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI32563869, Length=754, Percent_Identity=24.2705570291777, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=544, Percent_Identity=25, Blast_Score=119, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=571, Percent_Identity=24.3432574430823, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585251, Length=647, Percent_Identity=23.9567233384853, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 88631; Mature: 88500

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00396 TOPOISOMERASE_I_PROK ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSVAALSAKNKTLIVVESPSKAKTINKYLGSNYTVFASVGHIKDLPKKEIGLDFEHNY
CCCCCCHHCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHHCCHHHHCCCCCCCC
SPRYEIIPGKEKVVKQLKKLATEASNILIATDPDREGEAIAWHIANEIEHAKAPVARVLF
CCCEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHH
NEVTKKAILEAIEKPRHIDLRLVHSQQTRQGLDKIVGYKISPFLWKVVLRGLSAGRVQSV
HHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
ALRLICEREEEIERFVIQEYWTIAADFLTANKESFRARLVRLDGDKPEITNVEQAEAIAA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCHHHHHHHHH
IAKKGNYSVREITPRIQQRKQPLPFTTSLLQQAASNQLGFGAQRTMRTAQQLYEGIELGA
HHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
EGAMGLITYMRTDSTRISPEAVGEARNYIERNFGKDYVGAGSSGKPGKNAQDAHEAIRPT
CCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCH
SLLKTPEQVKPYLSADQFKLYELIWKRFLAAMMAPAKIEQTKVDVEEQSGKFLFRANGSR
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEEECCCE
VLFPGFMRVYDDQQELAYEAQTSTKEEVENEMVVKLPEKLAVNDPLGLGALEQKQSFTRP
EECCHHHHHHCCHHHHHHHHHCCCHHHHHCHHHHCCCHHHCCCCCCCCCCHHHHHHCCCC
PARYSEASLVKDLDHFGIGRPSTYASIFSTLQDRRYVALEKRKIMPTDLGRDVAKILVAN
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEHHCCCCCCCHHHHHHHHHHHHC
FPELFNVGFTAFMEDELDKVASGDDAYEKVLDSFYKPLTSALALRSATPLIPQNNEAETC
CHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
DKCGTGKMILKWTASGKFLGCSNYPKCKNIRTISSNREKPASTGVHCPSCEDGEMVLRKG
HHCCCCCEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHCC
RLGPFLACSNYPKCNTLLNLNKQRHIEPPKTPPVVTDMACPKCGAPLYLRSGKRGLWLGC
CCCCEEEECCCCCCCHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCEEEECCCCCEEECC
SKFPKCRGRLAWTALEPAAQERWERVMAAHQKAHPPVTLKMVDGSTVSMTSSIDDIIMKA
CCCCHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHC
DAAGLIAPAMDLVPEAEG
CCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ASSVAALSAKNKTLIVVESPSKAKTINKYLGSNYTVFASVGHIKDLPKKEIGLDFEHNY
CCCCCHHCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHHCCHHHHCCCCCCCC
SPRYEIIPGKEKVVKQLKKLATEASNILIATDPDREGEAIAWHIANEIEHAKAPVARVLF
CCCEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHH
NEVTKKAILEAIEKPRHIDLRLVHSQQTRQGLDKIVGYKISPFLWKVVLRGLSAGRVQSV
HHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
ALRLICEREEEIERFVIQEYWTIAADFLTANKESFRARLVRLDGDKPEITNVEQAEAIAA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCHHHHHHHHH
IAKKGNYSVREITPRIQQRKQPLPFTTSLLQQAASNQLGFGAQRTMRTAQQLYEGIELGA
HHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
EGAMGLITYMRTDSTRISPEAVGEARNYIERNFGKDYVGAGSSGKPGKNAQDAHEAIRPT
CCCHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCH
SLLKTPEQVKPYLSADQFKLYELIWKRFLAAMMAPAKIEQTKVDVEEQSGKFLFRANGSR
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCEEEEECCCE
VLFPGFMRVYDDQQELAYEAQTSTKEEVENEMVVKLPEKLAVNDPLGLGALEQKQSFTRP
EECCHHHHHHCCHHHHHHHHHCCCHHHHHCHHHHCCCHHHCCCCCCCCCCHHHHHHCCCC
PARYSEASLVKDLDHFGIGRPSTYASIFSTLQDRRYVALEKRKIMPTDLGRDVAKILVAN
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEHHCCCCCCCHHHHHHHHHHHHC
FPELFNVGFTAFMEDELDKVASGDDAYEKVLDSFYKPLTSALALRSATPLIPQNNEAETC
CHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
DKCGTGKMILKWTASGKFLGCSNYPKCKNIRTISSNREKPASTGVHCPSCEDGEMVLRKG
HHCCCCCEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHHHHCC
RLGPFLACSNYPKCNTLLNLNKQRHIEPPKTPPVVTDMACPKCGAPLYLRSGKRGLWLGC
CCCCEEEECCCCCCCHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCEEEECCCCCEEECC
SKFPKCRGRLAWTALEPAAQERWERVMAAHQKAHPPVTLKMVDGSTVSMTSSIDDIIMKA
CCCCHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHC
DAAGLIAPAMDLVPEAEG
CCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]