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The map label for this gene is emrB [H]

Identifier: 78188023

GI number: 78188023

Start: 42581

End: 44194

Strand: Reverse

Name: emrB [H]

Synonym: Cag_0039

Alternate gene names: 78188023

Gene position: 44194-42581 (Counterclockwise)

Preceding gene: 78188024

Following gene: 78188020

Centisome position: 1.72

GC content: 49.63

Gene sequence:

>1614_bases
ATGGCAAACGCACCTTCTCTTTCAGCCTCGCCAAAGCTACTCGGCACCACCGAAGAGCATTACGAAACAGGGTGGCGCAA
GCTCATTATCACCTTGACGGTTATTGTTTCCGCCATGCTGGAGCTGATTGACACCACCATTGTTAATGTGGCAATTACGC
AAATTAGCGGCAACCTTGGAGCCAGCATTGAGGACACCGCATGGGTGGTAACAAGCTACGCCATTGCAAACGTTATTGTA
ATTCCACTCTCAGGCTTTCTTGGCAATTTGCTTGGGCGGCGGAACTACTACATTGGCTCCATTCTGCTCTTTACCGTCGC
CTCTCTCCTGTGCGGCGTTGCAACCGACATTTGGACACTTGTCTTTTTCCGCTTTGTGCAAGGCATTGGCGGTGGCGCAC
TGCTCCCCACCTCGCAAGCCATTTTGTACGAAACCTTTCGCCCCGAAGAGCGCGGCAAAGCCACCGGTATTTTTTCAATG
GGCTTAGTGCTTGGACCAACCATTGGACCACTTTTAGGCGGCTACTTAGTAGATTATTTCAATTGGGAATGGTGCTTTTT
TGTCAACATTCCTATTGGACTGTTAGCTGCTTGGTCATCCTTTATTTTTCTTAAAGAGCCAAAAGTTACCCACACTGTCT
CAAAAATTGATTGGGCTGGAATTGGCTTACTTGCCGTTGGCATTGGTTCCCTGCAATTCATTTTAGAGCGAGGAGAATCC
AAAGATTGGTTTGAAACCCCCTACATTACATGGTTTACCATTATTGCCGTACTTTCGCTGATTGCGTTTGTATGGCACGA
ACTTCACACTAAGGAGCCTGCCGTTGACCTTCGTGTGCTGGCACGAAGCCACAATTTGCCCATTGCTGCCGTGCTCACCT
TTATTGTCGGCTTTGGTTTGTACGGTTCACTCTTTGTTTTTCCCGTTTTTGTGCAAGGGCTGCTTGGTTTTACCGCTGTG
CTCACCGGTTTAGTGCTCTTCCCCAGCGCTATGGTTACCGGTATGATTTCCATGCCACTTGGCATGGCGCTGCAAAAAGG
TGCCTCACCAAAGCATTTAATGCTCTTTGGAATGCTCACCTTTTCACTTTTTTGCTGGCTACTGGGGCAACAAACCTTGC
AATCAGGCGCCGAAAACTTTTTTTGGATATTGCTGCTTCGCGGCATTGCACTCGGCTTTATTTTCATTCCCGTTACCATG
CTCGCAATTTCGGGATTGCATGGCAAAGATATTGGACAGGCAACTGGCTTAAACAACATGGTGCGCCAACTTGGCGGCTC
ATTCGGCATTGCTATTGCCAACACCTACATCGCCAAACGAGTAGCCGCACACCGCACCGAGCTACTAAGCCATCTTTCGC
CTTACGACCCCGAAGCAATGAACCGCATACACGCCATTGCCGCCAAAGCCACTGCTGAACACGGGCTGCCACCCGCAAGC
GCCGAACTTGCCGCCCTGAAAGCGCTTGAAGGTACGGTAACCGTGCAAAGCACGCATCTTGCCTTTATGGATGCCTTTAT
GCTGATTGCTCTTCTTTTTCTCTGCGCTGTGCCACTACTCTTTTTTATTCGGCTGCATAAGGGGGAACAGGCAAGTGCAA
TGGGGGGGCATTGA

Upstream 100 bases:

>100_bases
CGCGTACCCGTTAAAATTCTCTTTACAGAAAAATTAGATAAACGCTACCCCCTTGCGGCTGGTATGAATGTAACCGTAGC
CGTTAAGGTGAAATAACGCT

Downstream 100 bases:

>100_bases
TTGGGGAAATGGTGATGAAGGGAAGGGAGAAAAAAAGTTTATAGATGTATTCCTTTGTTGTTTTTACCTAATGCTTTCTC
AATGGAATCTCTTCCCCGAT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MANAPSLSASPKLLGTTEEHYETGWRKLIITLTVIVSAMLELIDTTIVNVAITQISGNLGASIEDTAWVVTSYAIANVIV
IPLSGFLGNLLGRRNYYIGSILLFTVASLLCGVATDIWTLVFFRFVQGIGGGALLPTSQAILYETFRPEERGKATGIFSM
GLVLGPTIGPLLGGYLVDYFNWEWCFFVNIPIGLLAAWSSFIFLKEPKVTHTVSKIDWAGIGLLAVGIGSLQFILERGES
KDWFETPYITWFTIIAVLSLIAFVWHELHTKEPAVDLRVLARSHNLPIAAVLTFIVGFGLYGSLFVFPVFVQGLLGFTAV
LTGLVLFPSAMVTGMISMPLGMALQKGASPKHLMLFGMLTFSLFCWLLGQQTLQSGAENFFWILLLRGIALGFIFIPVTM
LAISGLHGKDIGQATGLNNMVRQLGGSFGIAIANTYIAKRVAAHRTELLSHLSPYDPEAMNRIHAIAAKATAEHGLPPAS
AELAALKALEGTVTVQSTHLAFMDAFMLIALLFLCAVPLLFFIRLHKGEQASAMGGH

Sequences:

>Translated_537_residues
MANAPSLSASPKLLGTTEEHYETGWRKLIITLTVIVSAMLELIDTTIVNVAITQISGNLGASIEDTAWVVTSYAIANVIV
IPLSGFLGNLLGRRNYYIGSILLFTVASLLCGVATDIWTLVFFRFVQGIGGGALLPTSQAILYETFRPEERGKATGIFSM
GLVLGPTIGPLLGGYLVDYFNWEWCFFVNIPIGLLAAWSSFIFLKEPKVTHTVSKIDWAGIGLLAVGIGSLQFILERGES
KDWFETPYITWFTIIAVLSLIAFVWHELHTKEPAVDLRVLARSHNLPIAAVLTFIVGFGLYGSLFVFPVFVQGLLGFTAV
LTGLVLFPSAMVTGMISMPLGMALQKGASPKHLMLFGMLTFSLFCWLLGQQTLQSGAENFFWILLLRGIALGFIFIPVTM
LAISGLHGKDIGQATGLNNMVRQLGGSFGIAIANTYIAKRVAAHRTELLSHLSPYDPEAMNRIHAIAAKATAEHGLPPAS
AELAALKALEGTVTVQSTHLAFMDAFMLIALLFLCAVPLLFFIRLHKGEQASAMGGH
>Mature_536_residues
ANAPSLSASPKLLGTTEEHYETGWRKLIITLTVIVSAMLELIDTTIVNVAITQISGNLGASIEDTAWVVTSYAIANVIVI
PLSGFLGNLLGRRNYYIGSILLFTVASLLCGVATDIWTLVFFRFVQGIGGGALLPTSQAILYETFRPEERGKATGIFSMG
LVLGPTIGPLLGGYLVDYFNWEWCFFVNIPIGLLAAWSSFIFLKEPKVTHTVSKIDWAGIGLLAVGIGSLQFILERGESK
DWFETPYITWFTIIAVLSLIAFVWHELHTKEPAVDLRVLARSHNLPIAAVLTFIVGFGLYGSLFVFPVFVQGLLGFTAVL
TGLVLFPSAMVTGMISMPLGMALQKGASPKHLMLFGMLTFSLFCWLLGQQTLQSGAENFFWILLLRGIALGFIFIPVTML
AISGLHGKDIGQATGLNNMVRQLGGSFGIAIANTYIAKRVAAHRTELLSHLSPYDPEAMNRIHAIAAKATAEHGLPPASA
ELAALKALEGTVTVQSTHLAFMDAFMLIALLFLCAVPLLFFIRLHKGEQASAMGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=444, Percent_Identity=32.4324324324324, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=30.0990099009901, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=24.3781094527363, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=26.634382566586, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082320, Length=198, Percent_Identity=25.2525252525253, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=485, Percent_Identity=24.7422680412371, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=388, Percent_Identity=26.5463917525773, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323735, Length=326, Percent_Identity=25.4601226993865, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=29.5454545454545, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=28.1045751633987, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=396, Percent_Identity=23.989898989899, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=361, Percent_Identity=24.9307479224377, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319783, Length=391, Percent_Identity=22.7621483375959, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58123; Mature: 57992

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANAPSLSASPKLLGTTEEHYETGWRKLIITLTVIVSAMLELIDTTIVNVAITQISGNLG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASIEDTAWVVTSYAIANVIVIPLSGFLGNLLGRRNYYIGSILLFTVASLLCGVATDIWTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFFRFVQGIGGGALLPTSQAILYETFRPEERGKATGIFSMGLVLGPTIGPLLGGYLVDYF
HHHHHHHCCCCCCCCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHC
NWEWCFFVNIPIGLLAAWSSFIFLKEPKVTHTVSKIDWAGIGLLAVGIGSLQFILERGES
CCCEEEEEECHHHHHHHHHHHEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
KDWFETPYITWFTIIAVLSLIAFVWHELHTKEPAVDLRVLARSHNLPIAAVLTFIVGFGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
YGSLFVFPVFVQGLLGFTAVLTGLVLFPSAMVTGMISMPLGMALQKGASPKHLMLFGMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSLFCWLLGQQTLQSGAENFFWILLLRGIALGFIFIPVTMLAISGLHGKDIGQATGLNNM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHH
VRQLGGSFGIAIANTYIAKRVAAHRTELLSHLSPYDPEAMNRIHAIAAKATAEHGLPPAS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AELAALKALEGTVTVQSTHLAFMDAFMLIALLFLCAVPLLFFIRLHKGEQASAMGGH
HHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ANAPSLSASPKLLGTTEEHYETGWRKLIITLTVIVSAMLELIDTTIVNVAITQISGNLG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASIEDTAWVVTSYAIANVIVIPLSGFLGNLLGRRNYYIGSILLFTVASLLCGVATDIWTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VFFRFVQGIGGGALLPTSQAILYETFRPEERGKATGIFSMGLVLGPTIGPLLGGYLVDYF
HHHHHHHCCCCCCCCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHC
NWEWCFFVNIPIGLLAAWSSFIFLKEPKVTHTVSKIDWAGIGLLAVGIGSLQFILERGES
CCCEEEEEECHHHHHHHHHHHEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
KDWFETPYITWFTIIAVLSLIAFVWHELHTKEPAVDLRVLARSHNLPIAAVLTFIVGFGL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
YGSLFVFPVFVQGLLGFTAVLTGLVLFPSAMVTGMISMPLGMALQKGASPKHLMLFGMLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSLFCWLLGQQTLQSGAENFFWILLLRGIALGFIFIPVTMLAISGLHGKDIGQATGLNNM
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHH
VRQLGGSFGIAIANTYIAKRVAAHRTELLSHLSPYDPEAMNRIHAIAAKATAEHGLPPAS
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
AELAALKALEGTVTVQSTHLAFMDAFMLIALLFLCAVPLLFFIRLHKGEQASAMGGH
HHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]