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The map label for this gene is gatB

Identifier: 78067927

GI number: 78067927

Start: 3623780

End: 3625255

Strand: Reverse

Name: gatB

Synonym: Bcep18194_A6458

Alternate gene names: 78067927

Gene position: 3625255-3623780 (Counterclockwise)

Preceding gene: 78067928

Following gene: 78067926

Centisome position: 98.14

GC content: 64.91

Gene sequence:

>1476_bases
ATGGCAACTCAATGGGAAGTCGTCATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGGGCGC
GTCGACGCAGTTCGGCGCCGAGCCGAACACGCAGGCCTGCCCGGTCGACCTGGCGCTGCCGGGCGTGCTGCCGGTGCTGA
ACCGCGGTGCGGTCGAACGCGCGATTCGCTTCGGCCTCGCGATTGGCTCGACCATCGCGCCGCGCAGCATCTTCGCGCGC
AAGAATTATTTCTACCCCGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCCAGAT
CACGATCCAGGTGCCTGCCAACGAAAAGGCCGGCAAGGACGCGTACGAAAAGACCGTCAACCTGACCCGCGCGCACCTCG
AGGAAGATGCGGGCAAGTCGCTGCATGAAGACTTCGCCGGGATGACGGGGATCGACCTGAACCGTGCGGGCACGCCGCTG
CTCGAGATCGTCACCGAGCCGGAAATGCGCAGCGCGGCCGAGGCCGTGGCCTACGCGAAGGCGCTGCACGGGCTCGTCGT
GTGGCTCGGCATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCGAACGTGTCGGTGCGCCCGATCGGCC
AGGAGAAGTTCGGCACGCGCGCGGAAATCAAGAACCTGAACTCGTTCCGGTTCCTCGAGGAAGCGATCAATTTCGAAGTG
CGTCGCCAGATCGAGCTGATCGAGGACGGCGGCGAAGTCGTCCAGGAAACGCGCCTCTACGATCCGGACAAGCGCGAGAC
GCGTTCGATGCGCAGCAAGGAAGACGCGCACGATTACCGCTACTTCCCCGACCCCGACCTGATGCCGCTCGTGATCGGCC
AGGACTGGATCGAGCGCGTGCAGTCCGGGATGCCCGAGCTGCCGGCCGCGATGCAGCAGCGCTTCGCCGACGAATACGGC
GTGTCCGCGTACGACGCGGGCGTGCTGACGTCGAGCAAGGCGATGGCCGCGTACTTCGAGGCCGTGGTCGCGAAGGCGGG
CACCGCGAACGCGAAGATCGCCGCGAACTGGCTGATGGGCGACGTTTCGTCGCAGCTGAACCGCGACGGCATCGAGATCG
ACGCGATTCCGGTCTCGGCTGCGCAGCTCGCACTCGTGCTGCAGCGCATCGCCGACGGCACGATCTCGAACAAGATCGCG
AAGGAAATCTTCACGACGATCTGGGACGAGAAGGCCGACGACGAAGGGGCGGCCGACCGCATCATCGACGCGAAGGGGCT
GAAGCAGATCTCCGACACCGGCGCGCTGGAAGCGATCATCGACGAAGTGCTCGCGGCGAACGCGAAGTCGGTCGAGGAAT
TCCGCGCGGGCAAGGAGAAGGCGTTCAACGCGCTGATCGGCCAGGCGATGAAGGCGACCAAGGGCAAGGCCAATCCGCAG
CAGGTCAACGAACTGCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
ATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCGGACGCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGT
TCCGGCGGGGGTGTAAGCAC

Downstream 100 bases:

>100_bases
GCATGACCGCGCGCCTGAACCGCGCATGACGACGGGCCGCCCCGAACTGTTCGGGGCGGCCCGTTGCGTTTGGTGGATGC
GTTATCGTATGCACCACAGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKDAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPIGQEKFGTRAEIKNLNSFRFLEEAINFEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGQDWIERVQSGMPELPAAMQQRFADEYG
VSAYDAGVLTSSKAMAAYFEAVVAKAGTANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALVLQRIADGTISNKIA
KEIFTTIWDEKADDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG

Sequences:

>Translated_491_residues
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKDAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPIGQEKFGTRAEIKNLNSFRFLEEAINFEV
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGQDWIERVQSGMPELPAAMQQRFADEYG
VSAYDAGVLTSSKAMAAYFEAVVAKAGTANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALVLQRIADGTISNKIA
KEIFTTIWDEKADDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
QVNELLKKKLG
>Mature_490_residues
ATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGSTIAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKDAYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVVWLGICDGNMQEGSFRCDANVSVRPIGQEKFGTRAEIKNLNSFRFLEEAINFEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGQDWIERVQSGMPELPAAMQQRFADEYGV
SAYDAGVLTSSKAMAAYFEAVVAKAGTANAKIAANWLMGDVSSQLNRDGIEIDAIPVSAAQLALVLQRIADGTISNKIAK
EIFTTIWDEKADDEGAADRIIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQQ
VNELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=38.6, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=31.3559322033898, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.7399617590822, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=34.20523138833, Blast_Score=321, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53508; Mature: 53377

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGSTIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKD
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCH
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHGLVVWLGICDGNMQEGSFRCDANVSVRPIGQEKFGTRAEIKNLNSFRFLEEAINFEV
HHHHHHHEEEECCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGQDWIERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHH
QSGMPELPAAMQQRFADEYGVSAYDAGVLTSSKAMAAYFEAVVAKAGTANAKIAANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALVLQRIADGTISNKIAKEIFTTIWDEKADDEGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
IIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC
>Mature Secondary Structure 
ATQWEVVIGLETHAQLSTVSKIFSGASTQFGAEPNTQACPVDLALPGVLPVLNRGAVER
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHH
AIRFGLAIGSTIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKD
HHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCH
AYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
ALHGLVVWLGICDGNMQEGSFRCDANVSVRPIGQEKFGTRAEIKNLNSFRFLEEAINFEV
HHHHHHHEEEECCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCHHHHHHHHHHCHHH
RRQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGQDWIERV
HHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHH
QSGMPELPAAMQQRFADEYGVSAYDAGVLTSSKAMAAYFEAVVAKAGTANAKIAANWLMG
HCCCCCCHHHHHHHHHHHHCCCHHCCCHHCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
DVSSQLNRDGIEIDAIPVSAAQLALVLQRIADGTISNKIAKEIFTTIWDEKADDEGAADR
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
IIDAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKEKAFNALIGQAMKATKGKANPQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLKKKLG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA