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The map label for this gene is gatA

Identifier: 78067928

GI number: 78067928

Start: 3625260

End: 3626750

Strand: Reverse

Name: gatA

Synonym: Bcep18194_A6459

Alternate gene names: 78067928

Gene position: 3626750-3625260 (Counterclockwise)

Preceding gene: 78067929

Following gene: 78067927

Centisome position: 98.18

GC content: 69.15

Gene sequence:

>1491_bases
ATGCACGCAAAAAGCCTGACTGAACTGCGCGCCGCGCTCGCCGCCAAGGAATGCTCGGCCGTCGAGCTCGCGCAGCTCTA
CCTGAAACGGATCGAAGCCGCGCGCGACCTGAACGCGTTCGTCCACGTCGATGCCGATCTGACCCTCGCGCAGGCGAAAG
CTGCCGACGCCGAGCTCGCGCGCGGCGCGGGCGGCGCGCTCACCGGCCTGCCGATCGCGCACAAGGATGTCTTCGTCACG
CGCGGCTGGCACTCGACCGCCGGCTCGAAGATGCTCGCGAACTACGAGAGCCCGTTCGACGCGACCGTCGTCGCCCGCCT
GCAGGCGGCCGGCATGGTCACGCTCGGCAAGACCAACATGGACGAGTTCGCGATGGGCTCGTCGAACGAGAATTCGGCGT
TCGGCGCGGTGAAGAACCCGTGGGACACCAACGCGGTGCCGGGCGGCAGCTCGGGCGGCAGCTCGGCCGCCGTCGCCGCG
CGCCTCGCGCCGGCCGCGACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCCGCGTCGTTCGCGGGCGTGACGGGCAT
CAAGCCGACCTACGGCCGCGTGTCGCGCTACGGGATGATCGCGTTCGCGTCGTCGCTCGACCAGGGCGGCCCGATGGCGC
AGAGCGCGTCCGACTGCGCGCTGCTGCTGAACGCGATGGCCGGCTTCGACGAGCGCGACTCGACGAGCCTCGAGCGCGAA
GACGAAGACTTCACGCGCCACCTCGGCCAGCCGTGGGCGGCCGGCAACGATGCAGGCAAGCCGCTCGCGGGCCTGCGCAT
CGGCCTGCCGAACGAGTATTTCGGCGACGGCCTCGCCGACGACGTGCGCGCGACGATCGACGCGGCCCTGAAGCAGTATG
AAGCGCTCGGCGCGACGCTCGTGCCCGTGTCGCTGCCCAAGACGGAACTGTCGATCCCCGTGTACTACGTGATCGCGCCG
GCGGAAGCGTCGTCGAACCTGTCGCGTTTCGACGGCGTGCGCTTCGGCCACCGCGCCGCGCAGTACGGCGACCTGCTCGA
CATGTACAAGAAGTCGCGCGCGGAAGGCTTCGGCCCCGAGGTGAAGCGCCGGATTCTGGTCGGCGCGTACGTGCTGTCGC
ACGGCTACTACGACGCGTACTACCTGCAGGCGCAGAAGATCCGCCGCATCATCGCGAACGATTTCCAGGAAGCGTTCAAG
TCCTGCGACGTGATCATGGGCCCGGCGTCGCCGACGGTCGCATGGGATCTCGGCTCGAAGGGTGACGATCCGGTGCAGAT
GTATCTCGCGGATATCTACACGCTGTCGGTGAGCCTCGCCGGCCTGCCCGGCATGAGCGTGCCGTGCGGCTTCGGTGCGG
GCGCGAACGCGAAGCGCCCGGTCGGCCTGCAGATCATCGGCAACTATTTCAACGAAGCCCGGATGCTGCAGGTCGCGGAC
GCGTTCCAGCGCGCGACCGACTGGCACAAGCAAGTTCCGGCGGGGGTGTAA

Upstream 100 bases:

>100_bases
CCTCTATCTTGTGCCGAAGGTGATCGAGTAAGCATTCGATGACAAGCGCGCCGCCGCCTTTGCGCCGCGCGCCACCCAGA
ATTCCCAGGAAAACCACTCA

Downstream 100 bases:

>100_bases
GCACATGGCAACTCAATGGGAAGTCGTCATCGGTCTCGAGACGCACGCGCAACTGTCGACCGTCTCGAAGATTTTCTCGG
GCGCGTCGACGCAGTTCGGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MHAKSLTELRAALAAKECSAVELAQLYLKRIEAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWHSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERE
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGDGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGSKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Sequences:

>Translated_496_residues
MHAKSLTELRAALAAKECSAVELAQLYLKRIEAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWHSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERE
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGDGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGSKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV
>Mature_496_residues
MHAKSLTELRAALAAKECSAVELAQLYLKRIEAARDLNAFVHVDADLTLAQAKAADAELARGAGGALTGLPIAHKDVFVT
RGWHSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNMDEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAA
RLAPAATGTDTGGSIRQPASFAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERE
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGDGLADDVRATIDAALKQYEALGATLVPVSLPKTELSIPVYYVIAP
AEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPEVKRRILVGAYVLSHGYYDAYYLQAQKIRRIIANDFQEAFK
SCDVIMGPASPTVAWDLGSKGDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
AFQRATDWHKQVPAGV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=37.3046875, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=25.7545271629779, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI166795287, Length=135, Percent_Identity=38.5185185185185, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=473, Percent_Identity=37.2093023255814, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=32.3529411764706, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=30.7291666666667, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=323, Percent_Identity=27.2445820433437, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=229, Percent_Identity=30.1310043668122, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=31.7708333333333, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=31.7708333333333, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=444, Percent_Identity=34.009009009009, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=424, Percent_Identity=28.5377358490566, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=533, Percent_Identity=22.3264540337711, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=40.122199592668, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=26.1386138613861, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=26.1386138613861, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=26.1386138613861, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=26.1386138613861, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24644968, Length=521, Percent_Identity=24.5681381957774, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078093, Length=531, Percent_Identity=25.6120527306968, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=509, Percent_Identity=23.1827111984283, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648435, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648437, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550774, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52393; Mature: 52393

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAKSLTELRAALAAKECSAVELAQLYLKRIEAARDLNAFVHVDADLTLAQAKAADAELA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
RGAGGALTGLPIAHKDVFVTRGWHSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERE
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGDGLADDVRATIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGAYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHAKSLTELRAALAAKECSAVELAQLYLKRIEAARDLNAFVHVDADLTLAQAKAADAELA
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
RGAGGALTGLPIAHKDVFVTRGWHSTAGSKMLANYESPFDATVVARLQAAGMVTLGKTNM
CCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCEEEECCCCH
DEFAMGSSNENSAFGAVKNPWDTNAVPGGSSGGSSAAVAARLAPAATGTDTGGSIRQPAS
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCH
FAGVTGIKPTYGRVSRYGMIAFASSLDQGGPMAQSASDCALLLNAMAGFDERDSTSLERE
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DEDFTRHLGQPWAAGNDAGKPLAGLRIGLPNEYFGDGLADDVRATIDAALKQYEALGATL
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCEE
VPVSLPKTELSIPVYYVIAPAEASSNLSRFDGVRFGHRAAQYGDLLDMYKKSRAEGFGPE
EEEECCCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
VKRRILVGAYVLSHGYYDAYYLQAQKIRRIIANDFQEAFKSCDVIMGPASPTVAWDLGSK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCCC
GDDPVQMYLADIYTLSVSLAGLPGMSVPCGFGAGANAKRPVGLQIIGNYFNEARMLQVAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AFQRATDWHKQVPAGV
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA