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The map label for this gene is gacA [H]

Identifier: 78067872

GI number: 78067872

Start: 3571445

End: 3572260

Strand: Direct

Name: gacA [H]

Synonym: Bcep18194_A6403

Alternate gene names: 78067872

Gene position: 3571445-3572260 (Clockwise)

Preceding gene: 78067871

Following gene: 78067873

Centisome position: 96.68

GC content: 66.79

Gene sequence:

>816_bases
ATGAGCCTGAACATCCTGCTCGTGGACGATCACGCGATCGTCCGGCAAGGGATCCGCCAGTTGCTGATCGACCGTGGCGT
CGCACGCGACGTCACCGAGGCCGAGAACGGCGGCGACGCCGTGGCCGCCGTCGACAAGCAGGAATTCGACGTGATCCTGC
TCGACATCTCGCTGCCGGACACGAACGGCATCGAAGTGCTCAAGCGCCTCAAGCGCAAGCTGCCGCGCACGCCGGTGCTG
ATGTTCTCGATGTACCGCGAGGATCAGTACGCGGTGCGCGCGCTGAAGGCCGGCGCCGCCGGCTATCTGTCGAAGACGGT
CAACGCCGCGCAGATGATCGGCGCGATCCAGCAGGTCGCGGCCGGCCGCAAGTACGTGAGCCCCGCGATGGCCGAGGCGC
TCGCCGAATACGTGTCGTTCGAGAACGAGCCGCTGCCGCACGAGAAGCTGTCCGATCGCGAATACCAGACGCTCTGCATG
CTCGCGTCGGGCAAGCGGCTCACCGACATCGCGAACACGCTGTCGCTGTCCGTGAAGACGGTGAGCGTGTACCGGTCGCG
GCTGCTCGAGAAGATGCGGCTGTCGAACAACGCGGAGCTCACGTTCTACGTGATGAGCAACCGGCTCGTCGACATGGCGC
CCACGGCCGGCGCCGGATCTTCCCGCCCGACTCACCCGGTTCACCCGATCCCGCGACGCGGCGCATGCCGCGCCGCCGCC
CGCGGCCGCCCGCCCGATGCGCAAGCGCTTGTTTTATCGGACGATTTTGTGACGCACCGCTGCGCTGCAGCGGGTCTTGT
GCGCCAAATCGGCTAA

Upstream 100 bases:

>100_bases
GACGCCGGCCACGGCACGCGCGTCACCGCCCGCTTCGCTTGGGATTCGCTGCTCGCGGTGCCCGTCGCGGCCCGCCGCGC
GTCGCTTTCGTGAGGTCGTC

Downstream 100 bases:

>100_bases
AATTACGGGTTTTCACTCAGTCAGCGCTACCGCGCCTGCGCGCGCTGGCGATTCATCCGCAATGTCTCTCTTCCGCAAGA
AAAACGTCGATCGCATGATC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAVAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLPRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGAGSSRPTHPVHPIPRRGACRAAA
RGRPPDAQALVLSDDFVTHRCAAAGLVRQIG

Sequences:

>Translated_271_residues
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAVAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLPRTPVL
MFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGAGSSRPTHPVHPIPRRGACRAAA
RGRPPDAQALVLSDDFVTHRCAAAGLVRQIG
>Mature_270_residues
SLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAVAAVDKQEFDVILLDISLPDTNGIEVLKRLKRKLPRTPVLM
FSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCML
ASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGAGSSRPTHPVHPIPRRGACRAAAR
GRPPDAQALVLSDDFVTHRCAAAGLVRQIG

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=34.2857142857143, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=36.8686868686869, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=28.5024154589372, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=30.9178743961353, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1786747, Length=213, Percent_Identity=30.5164319248826, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787473, Length=210, Percent_Identity=32.8571428571429, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=25.9615384615385, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=35, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788191, Length=113, Percent_Identity=31.858407079646, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 29612; Mature: 29480

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAVAAVDKQEFDVILLDISLPD
CCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC
TNGIEVLKRLKRKLPRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGAGSSRPTHPVHPIPRRGACRAAA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHC
RGRPPDAQALVLSDDFVTHRCAAAGLVRQIG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLNILLVDDHAIVRQGIRQLLIDRGVARDVTEAENGGDAVAAVDKQEFDVILLDISLPD
CEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCC
TNGIEVLKRLKRKLPRTPVLMFSMYREDQYAVRALKAGAAGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPTAGAGSSRPTHPVHPIPRRGACRAAA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHC
RGRPPDAQALVLSDDFVTHRCAAAGLVRQIG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]