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The map label for this gene is miaB [H]

Identifier: 78067490

GI number: 78067490

Start: 3155195

End: 3156691

Strand: Direct

Name: miaB [H]

Synonym: Bcep18194_A6021

Alternate gene names: 78067490

Gene position: 3155195-3156691 (Clockwise)

Preceding gene: 78067489

Following gene: 78067491

Centisome position: 85.41

GC content: 65.13

Gene sequence:

>1497_bases
ATGGAAGGCTTTCCCGACGGCTTTTCATGCTGGCTGCGCGCGATCCGCGTGCAGCGCACGGCCGTCCCGATTCACCCTAT
AGACGACGCCCCCAGGTCAACCACTGCGAACGGCGAACCCCCCATGACCAAGAAAGTTTACGTAAAGACCTTCGGCTGCC
AGATGAACGAGTACGACTCGGACAAGATGGTGGACGTGCTGAATGCGGCCGAAGGCCTCGAAAAGACCGATACGCCGGAA
GACGCGGACATCATCCTGTTCAACACCTGCTCGGTGCGTGAAAAGGCGCAGGAGAAGGTGTTCTCCGATCTCGGCCGCGT
GCGCGAGCTGAAGGAAGCGAAGCCCGGCCTGCTGATCGGCGTCGGCGGCTGCGTCGCGAGCCAGGAAGGCGCATCGATCG
TGTCGCGCGCGCCGTACGTCGACCTCGTGTTCGGCCCGCAAACCCTGCACCGCCTGCCGCAGATGATCGACGCGCGCCGC
GAGAGCGGCCGTGCGCAGGTCGACATCTCGTTCCCCGAAATCGAGAAGTTCGACCACCTGCCGCCCGCGCGCGTCGAGGG
GCCGAGCGCATTCGTGTCGATCATGGAAGGCTGCTCGAAGTACTGCAGCTACTGCGTGGTGCCGTACACGCGCGGCGATG
AAGTGTCGCGCCCGCTCGACGACGTACTGACCGAAGTGGCCGGCCTCGCCGACCAGGGCGTGCGCGAAGTCACGCTGCTC
GGCCAGAACGTGAACGCGTATCGCGGCGCGCTGGCGGCCGGCTCGTCGGAAATCGCCGATTTCGCAACGCTGATCGAATA
CGTCGCCGACATCCCCGGCATCGAGCGGATCCGCTACACGACGTCGCACCCGAAGGAATTCACGCAGCGCCTGATCGACA
CCTACGCGAAGGTGCCGAAGCTCGTGAACCACCTGCACCTGCCCGTCCAGCACGGCTCCGACCGCATCCTGATGGCGATG
AAGCGCGGCTACACGGTGCTCGAATACAAGTCGGTGATCCGCAAGCTGCGCGCGATCCGCCCGGACCTGTCGCTGTCGAC
CGACATGATCGTCGGCTTCCCCGGCGAGACCGAGGACGATTTCGACAAGATGATGGCGCTCGTGCACGAGATGAGCTACG
ACACCAGCTTCTCGTTCATCTACAGCCCGCGCCCCGGCACACCGGCCGCGAACCTGCACGACGACACGCCGCGCGACGTG
AAGCTCAAACGCCTGCAACATCTGCAGGCGACCATCGAGGAAAACGTCGCGCGCATCAGCCAGTCGATGGTCGGGAAGGT
CGAGCGGATCCTCGTCGAGGGCCCGTCGCGCAAGGACCCGAACGAACTCGCGGGCCGCACGGAGAACAACCGGGTCGTGA
ATTTCCCGGCGCCGCTCGCGTCGCACCCGCGCCTGATCGGCCAGATGATCGACGTGAAGATCAACCATGCGTACCCGCAT
TCGCTGCGCGGCGAGCTCGTGCTCGTCAGCGACGACGCGAGCGCGGCCACCCACTGA

Upstream 100 bases:

>100_bases
CGCTGACGGCCGGCACGCCACCATTCAAGGGTCGGAACGTGACGGACGGCGTCGCGCGGCCGCAACCCGCCGCCCGTCCC
GACCAGCATGAAAAGCTATA

Downstream 100 bases:

>100_bases
CTGACGCTCAACAGGAACCCGACGCCACTTTGAAGCCGACCCAAGCACTGGAATTCGTCGCGCCGCGCGACGACAACGCG
CGCCTCGCCAACCTCTGCGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEGFPDGFSCWLRAIRVQRTAVPIHPIDDAPRSTTANGEPPMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPE
DADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARR
ESGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLL
GQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAM
KRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDV
KLKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPH
SLRGELVLVSDDASAATH

Sequences:

>Translated_498_residues
MEGFPDGFSCWLRAIRVQRTAVPIHPIDDAPRSTTANGEPPMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPE
DADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARR
ESGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLL
GQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAM
KRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDV
KLKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPH
SLRGELVLVSDDASAATH
>Mature_498_residues
MEGFPDGFSCWLRAIRVQRTAVPIHPIDDAPRSTTANGEPPMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPE
DADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARR
ESGRAQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLL
GQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAM
KRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDV
KLKRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPH
SLRGELVLVSDDASAATH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.8200836820084, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.8200836820084, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI93277076, Length=470, Percent_Identity=28.0851063829787, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=60.9756097560976, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI1787057, Length=457, Percent_Identity=29.5404814004376, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=505, Percent_Identity=34.4554455445545, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71996771, Length=321, Percent_Identity=28.0373831775701, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=32.9896907216495, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922432, Length=473, Percent_Identity=28.3298097251586, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55172; Mature: 55172

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGFPDGFSCWLRAIRVQRTAVPIHPIDDAPRSTTANGEPPMTKKVYVKTFGCQMNEYDS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
DKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIG
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDISFPEIEKFDHL
ECCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
PPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLL
CCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
LVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEDD
HHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHH
FDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDVKLKRLQHLQATIEENVARIS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPH
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHEEECCCCCC
SLRGELVLVSDDASAATH
CCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MEGFPDGFSCWLRAIRVQRTAVPIHPIDDAPRSTTANGEPPMTKKVYVKTFGCQMNEYDS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
DKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPGLLIG
HHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRESGRAQVDISFPEIEKFDHL
ECCHHCCCCCHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
PPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLL
CCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTSHPKEFTQRLIDTYAKVPK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
LVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDMIVGFPGETEDD
HHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHH
FDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDVKLKRLQHLQATIEENVARIS
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASHPRLIGQMIDVKINHAYPH
HHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHEEECCCCCC
SLRGELVLVSDDASAATH
CCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA