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The map label for this gene is kefA [H]

Identifier: 78067309

GI number: 78067309

Start: 2953962

End: 2955326

Strand: Reverse

Name: kefA [H]

Synonym: Bcep18194_A5840

Alternate gene names: 78067309

Gene position: 2955326-2953962 (Counterclockwise)

Preceding gene: 78067310

Following gene: 78067308

Centisome position: 80.0

GC content: 67.91

Gene sequence:

>1365_bases
ATGGAGAATCTGCAGAGTCGTCTGCTGTCGCACCGGCTGGCGTCGGTCATCCGCGATTTCCACCAGCCGGTGATGATCTG
GCAGGCGGCGATCCTCATCGGTGCGCTGTGCTTCGCCTGGGTGGCCGCGCGCTTCGTGCACGGCCGGATCGACGCGCGCC
GCCGGGCGGCCGGACGTGCGCCCGGCGCGGGTGCGCAAAGCCTGAAGCGCGCGTTGTTCCCGCTGTTCGGCAGCGTGTTC
GTGGGCGGCGCGCAGCTCGCGTTCGACCCGTTCATGTCGACGTCGCTGCTGTCGCTCGCGCTCGTGCCGCTGTTCGGCAT
CGGGCTGATCTACGTGCTGTTCTTCTTTGCGCGACGCGTGTTTGCGCGCGATGGCCACACGCATGCGTGGCTGTCGATCG
TCGAGAAGATCGTGTCGACTGTCGTGTGGGCCGCGATGGTGCTGACCGTGCTCGGCATCCAGCGTGACGTGCTCGGCTGG
CTCGACAGCGTGCAGTTCCGTGTCGCCAATGCGCACCTCACGCTGCTGTCGGTGATTTCCGGCGCGTTGTGGGTCTGCGT
GACGCTGATGGTCGCGATGTGGCTCGGCTCGGTGCTCGAGGATCGCCTCACGCGCGCGACCACGCTCGACGCGAACCTGA
AGGTCGTGCTGTCGCGGGTCGGCCGAGCGCTGCTCGTGTTCGCGGCGATCCTGATCGGGCTGTCGCTGGTCGGCATCGAC
GTGACGGTGCTCGGCGTGTTCGGCGGCGCGGTGGGTGTCGGCCTCGGTTTCGGGCTGCAGAAGATCGCCAGCAACTACGT
GTCGGGCTTCATCATCCTTCTCGACCGCTCGCTGCGGCTCGGCGACGCGATCAGCGTCGGCGGGCTGCAGGGCGTCGTCA
CGCAGATCCGCACGCGCTACACGGTCGTGCGCGGCCTCGACGGCAACGAGACGCTGATCCCGAACGAAAAGCTGATCACC
GACGTCGTGCAGAACCAGTCGTCGTACCTGACACGCGGCTACGCGAAGGTCGCGGTGCAGGTCGCGTACACGAGCGATGT
CGAGTACGCGCTCACGCTGCTCGCCGATGCGGCCAAGGGCGTCCCGCGTGTGCTGGCGGAGCCCGCTCCGACCCCGTATC
TGGTGAGTTTCGGGGCCGACGGGATCGACCTGGAGCTCGGTTTCTGGATCGAGGATTCCGCGAAAGGCACGTCCGGCGTG
CGTTCGACCGTGAACCGCAACATCTGGCGCCTGTTCAGCGAGCACGGGATTTCGATTCCGTTCCCGCAGCGCGAAGTGCG
CGTCGTCGGGTTGCCCGACGGCTTCCCCGCAGCGGGCGCGGCGGCCGGCAACGGCGCCGTGGACGGGCACGTACCGGCTG
CATGA

Upstream 100 bases:

>100_bases
CGACTACCTGTCGCTATGGCCGCACCGCCACGCGACCGACGGCTTCTTCGCCGCGGTGCTCGAACGGCGCGCTGCGCAGT
AACGGCGGGCCGGACGGACG

Downstream 100 bases:

>100_bases
CGCGCGCCGGTCGCCAATCGTATCGACCCCGCGGTCGGAACCGGATCGCGGGCCTTTCCAAGTCCGCCCATGGACTGGGT
TTGTTGCGTCAGGGCAAGAA

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MENLQSRLLSHRLASVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVLTVLGIQRDVLGW
LDSVQFRVANAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLTRATTLDANLKVVLSRVGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEYALTLLADAAKGVPRVLAEPAPTPYLVSFGADGIDLELGFWIEDSAKGTSGV
RSTVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAGAAAGNGAVDGHVPAA

Sequences:

>Translated_454_residues
MENLQSRLLSHRLASVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVLTVLGIQRDVLGW
LDSVQFRVANAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLTRATTLDANLKVVLSRVGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEYALTLLADAAKGVPRVLAEPAPTPYLVSFGADGIDLELGFWIEDSAKGTSGV
RSTVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAGAAAGNGAVDGHVPAA
>Mature_454_residues
MENLQSRLLSHRLASVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVLTVLGIQRDVLGW
LDSVQFRVANAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLTRATTLDANLKVVLSRVGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEYALTLLADAAKGVPRVLAEPAPTPYLVSFGADGIDLELGFWIEDSAKGTSGV
RSTVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAGAAAGNGAVDGHVPAA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=227, Percent_Identity=36.1233480176211, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI2367355, Length=262, Percent_Identity=30.9160305343511, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789291, Length=263, Percent_Identity=27.3764258555133, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 48567; Mature: 48567

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]