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The map label for this gene is hutC [H]

Identifier: 78066950

GI number: 78066950

Start: 2566910

End: 2567605

Strand: Reverse

Name: hutC [H]

Synonym: Bcep18194_A5481

Alternate gene names: 78066950

Gene position: 2567605-2566910 (Counterclockwise)

Preceding gene: 78066951

Following gene: 78066949

Centisome position: 69.51

GC content: 66.09

Gene sequence:

>696_bases
ATGAGCGCGCCGGTCTACCAGGAGATCAAGGATTTCATCCTGGGCCGCATCCACGCAGGCGAGTGGGAGGAGGGCGATCA
GGTGCCGTCCGAGAACGAGTTGGCGCGCGAATTCAAGGTGGCGCGCATGACCGTCAACCGCGCGTTGCGCGAGTTGACGG
CCGAGCAGGTGCTCACCCGCATGAAGGGGGCGGGCACCTACGTCGCGCGGCCGAAGTACGAATCGACGCTGGTGGCGATC
CGCAGCATCTCGGAGGAAGTCGGTGCACGCGGGCATGCGTATCACGCCAGCGTCCTCGGCCTCGACACGATCCGCGCCGA
CGAGGCGCTCGCCGACGAGATGCAGGTCGCCGTGCGCACGAAGCTGTTTCATTCGCAGGTGCTGCACTTCGAGAATGACG
AGCCCGTGCAGCTTGAAGAACGATGGGTGAATCCGGCGGTCGCGCCGGATTACGCCGAGCAGGATTTCACGAACACGACG
CCGAACCTGTACCTGATGCGCGCGGCTCCGCTGCAGCGCGTCGAGTACCGGATCGAAGCGGCGGCCCCGGCGCCGGAGCG
GCGCGAGCAGCTGCGGATGGACGACGTCGAGCCGTGTCTGGTTTTACATCGACGCACCTGGTCGCAGGGCGTCGTCGCCT
CGGTGGCGAATCTGTGGCATCCCGGCAGCCGATACCGCTTCACCGGGCATTTCTGA

Upstream 100 bases:

>100_bases
CACTACTTCGCACCGGATATCGCGGTGATCGCGAAGCTGGTCGGCGAGCGTGCGTTCGCGAAGGTCGCGCCGTTCTCGTT
CGCGTCGGAACAGTAAGCCC

Downstream 100 bases:

>100_bases
TTCCTATTTGATATTCATTTTCCTTCGGGAGCAGTCGTCATGAACCATCCGAAACACATCGATCCCCGTCTCGATCCGAC
CCGCACGATCCGTGCGCCGC

Product: histidine utilization repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSAPVYQEIKDFILGRIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVGARGHAYHASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTT
PNLYLMRAAPLQRVEYRIEAAAPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Sequences:

>Translated_231_residues
MSAPVYQEIKDFILGRIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAI
RSISEEVGARGHAYHASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTT
PNLYLMRAAPLQRVEYRIEAAAPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
>Mature_230_residues
SAPVYQEIKDFILGRIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTRMKGAGTYVARPKYESTLVAIR
SISEEVGARGHAYHASVLGLDTIRADEALADEMQVAVRTKLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTP
NLYLMRAAPLQRVEYRIEAAAPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=198, Percent_Identity=30.3030303030303, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082252, Length=149, Percent_Identity=26.1744966442953, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788418, Length=211, Percent_Identity=25.5924170616114, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790118, Length=139, Percent_Identity=24.4604316546763, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 26313; Mature: 26182

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPVYQEIKDFILGRIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVGARGHAYHASVLGLDTIRADEALADEMQVAVRT
HCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTPNLYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHC
AAPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
SAPVYQEIKDFILGRIHAGEWEEGDQVPSENELAREFKVARMTVNRALRELTAEQVLTR
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGAGTYVARPKYESTLVAIRSISEEVGARGHAYHASVLGLDTIRADEALADEMQVAVRT
HCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFHSQVLHFENDEPVQLEERWVNPAVAPDYAEQDFTNTTPNLYLMRAAPLQRVEYRIEA
HHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHC
AAPAPERREQLRMDDVEPCLVLHRRTWSQGVVASVANLWHPGSRYRFTGHF
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2203754; 2834335 [H]