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The map label for this gene is puuA [H]

Identifier: 78066942

GI number: 78066942

Start: 2558183

End: 2559520

Strand: Reverse

Name: puuA [H]

Synonym: Bcep18194_A5473

Alternate gene names: 78066942

Gene position: 2559520-2558183 (Counterclockwise)

Preceding gene: 78066943

Following gene: 78066941

Centisome position: 69.29

GC content: 64.72

Gene sequence:

>1338_bases
ATGCAACCCGAACTGAGCGAATTCCTGCGACAGCACCGCATCACCGAGGTCGAGGCGATCATTCCCGACATGGCCGGCAT
TGCGCGCGGCAAGATCATTCCGCGCAACAAGTTCGAATCCGGCGAATCGATGCGCCTGCCGCAGGCCGTGATGGTGCAGA
CCGTCACCGGCGACTATCCGGAGGACGGCACGCTGACCGGCGTGACCGACCCCGACATGGTGTGCGTGCCCGATCCGTCG
ACGATCTGCCTGATTCCGTGGGCCGTCGACCCGACCGCGCAGGTGATCCACGACTGCGTGCACTTCGACGGCTCGCCGGT
CGAGATCTCGCCGCGCTATGTGTTGCGACGCGTACTCGATCTTTACAAGGAGAAGGGCTGGCAGCCGGTCGTCGCGCCCG
AGCTCGAGTTCTATCTCGTCGACATGAATGCCGATCCCGACCTGCCGCTGCGTCCGCCGGTCGGCCGCACCGGGCGTGCG
GAGACCGGCCGGCAGTCGTATTCGATCGAGGCCGTGAACGAGTTCGATTCGCTGTTCGAGGATATCTACGAATATTGCGA
GATGCAGGGGCTCGATATCGAGACGCTGATCCACGAGGTCGGTGCCGCGCAGATGGAGATCAACTTCGTGCACGGCGATG
CGCTGTCGCTCGCCGACCAGGTGTTCCTGTTCAAGCGCACCGTGCGCGAGGCGGCGCTGCGCCACAACATGTACGCGACC
TTCATGGCGAAGCCGATGCAGGACGAGCCCGGCTCCGCGATGCACATCCACCAGAGCCTGGCCGACGCGCGCACGGGGCG
CAACCTGTTCTCCAGCGAGGACGGTGCCGTGTCGCCGATGTTCCACAGCTATCTCGCGGGGCTGCAGAAGTACACGCCCG
CGTTGATGCCGATCTTTGCGCCGTACATCAACTCGTATCGCCGCCTGTCGCGCTTCATGGCGGCGCCGATCAACGTGCAG
TGGGGTTACGACAACCGCACGGTCGGTTTCCGCATTCCGCAGTCGGGCGCATCGGCCCGCCGCATCGAGAACCGGATCCC
GGGCGTCGACTGCAATCCGTATCTCGCCTTCGCCGCGACGCTCGCGGCCGGCTATCTCGGCTTGACCCAGCAGCTCGCAC
CGACCGAGCCGATTGCGTCGGACGGCTACAAGCTGCCGTACCAGCTGCCGCGCAATCTCGAGGAGGGCATCTCGCTGATG
GCGGCCTGCACGCCGCTGGCCGGCATTCTCGGCGACAAGTTCGTGAAAGCGTACCTGGCGCTGAAGGACACCGAATACGA
GGCGTTTTTCCGCGTGATCAGCTCGTGGGAACGCAGGCATCTGCTGCTGCACGTGTGA

Upstream 100 bases:

>100_bases
TGGCGCGGCGTGCCGCGCGCGCATGACGCATCGCATTCATGCAGCCGGCGGCGCGATGGCGTCGCCGGCTGCATCCGACG
TCGACTGATAGAGGCCGATC

Downstream 100 bases:

>100_bases
GCGTGCGGCACTGTTATCCGGAGGAAACATGACCGATCGAAACGAAGCTGTCCCACCGCATTCGACCGCGGACTATCGCG
CGCTCGACGCCGCACACCAC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKEKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDSLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMQDEPGSAMHIHQSLADARTGRNLFSSEDGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSGASARRIENRIPGVDCNPYLAFAATLAAGYLGLTQQLAPTEPIASDGYKLPYQLPRNLEEGISLM
AACTPLAGILGDKFVKAYLALKDTEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKEKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDSLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMQDEPGSAMHIHQSLADARTGRNLFSSEDGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSGASARRIENRIPGVDCNPYLAFAATLAAGYLGLTQQLAPTEPIASDGYKLPYQLPRNLEEGISLM
AACTPLAGILGDKFVKAYLALKDTEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVPDPS
TICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKEKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRA
ETGRQSYSIEAVNEFDSLFEDIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
FMAKPMQDEPGSAMHIHQSLADARTGRNLFSSEDGAVSPMFHSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPQSGASARRIENRIPGVDCNPYLAFAATLAAGYLGLTQQLAPTEPIASDGYKLPYQLPRNLEEGISLM
AACTPLAGILGDKFVKAYLALKDTEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=434, Percent_Identity=26.4976958525346, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=34.9665924276169, Blast_Score=247, Evalue=8e-67,
Organism=Escherichia coli, GI1790301, Length=458, Percent_Identity=28.3842794759825, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49825; Mature: 49825

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLD
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYKEKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDSLFE
HHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMQDEPGSAMHIHQSLADARTGRNLFSSEDGAVSPMFHSYLAGLQKYTPALMPIFA
HHCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSGASARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGLTQQLAPTEPIASDGYKLPYQLPRNLEEGISLMAACTPLAGILGDKFVKAYLA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDTEYEAFFRVISSWERRHLLLHV
HCCCHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQPELSEFLRQHRITEVEAIIPDMAGIARGKIIPRNKFESGESMRLPQAVMVQTVTGDYP
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCC
EDGTLTGVTDPDMVCVPDPSTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLD
CCCEEECCCCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHH
LYKEKGWQPVVAPELEFYLVDMNADPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDSLFE
HHHHCCCCCEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
DIYEYCEMQGLDIETLIHEVGAAQMEINFVHGDALSLADQVFLFKRTVREAALRHNMYAT
HHHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMAKPMQDEPGSAMHIHQSLADARTGRNLFSSEDGAVSPMFHSYLAGLQKYTPALMPIFA
HHCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPQSGASARRIENRIPGVDCNPYLAFAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGLTQQLAPTEPIASDGYKLPYQLPRNLEEGISLMAACTPLAGILGDKFVKAYLA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDTEYEAFFRVISSWERRHLLLHV
HCCCHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]