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The map label for this gene is bioA [C]

Identifier: 78066941

GI number: 78066941

Start: 2556769

End: 2558154

Strand: Reverse

Name: bioA [C]

Synonym: Bcep18194_A5472

Alternate gene names: 78066941

Gene position: 2558154-2556769 (Counterclockwise)

Preceding gene: 78066942

Following gene: 78066938

Centisome position: 69.25

GC content: 65.51

Gene sequence:

>1386_bases
ATGACCGATCGAAACGAAGCTGTCCCACCGCATTCGACCGCGGACTATCGCGCGCTCGACGCCGCACACCACATCCACCC
GTTCTCTGACATGGGCGCGCTGAACCGCGCGGGCAGCCGCGTGATCGTGAAAGCCGACGGCGTCTACCTGTGGGACTCGG
ACGGCAACAAGATCATCGACGGGATGGCCGGCCTCTGGTGCGTGAGCGTCGGCTACGGCTGCAAGGCACTCGCCGACGCC
GCGTATCGCCAGATCCAGGAACTGCCGTTCTACAACACGTTCTTCAAGACCACGCACCCGCCGGTGATCGAACTGTCCGC
GATGCTCGCGGAAGTGTCGCCGAAGGGCTTCAACCACTTCTTCTACTGCAACAGCGGCTCGGAAGGCAACGACACCGTGC
TGCGCCTCGTGCACCAGTACTGGCGCGTGCAGGGCCAGCCGCAGAAGAAGTATGTGATCTCGCGCAAGAACGGCTATCAC
GGCTCGACGATCGCGGGCGGTACGCTCGGCGGGATGGGCTACATGCACGAGCAGATGCCGTCGAAGGTCGAGAACATCGT
GCACATCGACCAGCCGTATTTCTTCGGCGAAGCGGCAGCCGGTGAAACGCCGGAAGCGTTCGGCCTTGCCCGCGCGCAGC
AGCTCGAAGCGAAGATCCTCGAACTCGGCGCGGAGAACGTCGCCGCGTTCATCGGCGAGCCGTTCCAGGGCGCGGGCGGC
GTGATCTTCCCGCCGTCGACGTACTGGCCGGAAATCCAGCGGATCTGCCGCAAGTACGACATCCTGCTCGCGGCCGACGA
AGTGATCGGCGGCTTCGGCCGTACTGGCGAATGGTTCGCACACCAGCATTTCGGCTTCGAGCCTGACCTCATCACGATGG
CGAAGGGCCTCACGTCGGGTTACGTGCCGATGGGCGCCGTCGGCATTCACGAGCGCGTGGCGCGACCGATCATCGACAAC
GGCGAATTCAACCACGGCCTCACGTACTCGGGCCACCCGGTTGCGGCGGCCGTCGCGGTCGCGAACCTGAAGCTGCTGCG
CGACGAAGGGATCGTCGAGCGCGTGAAGAACGACATCGGGCCGTACTTCCAGCGCCGGCTGCGCGATGCGCTGGGCGATC
ATCCGATCGTCGGAGAGATTGCGGGAGCGGGGCTGGTCGCGGGCGTTCAGCTTGCGCGCGACCGGGACCGGCGCGAGCGG
TTCGGTGCGAGCGTCGATATCGGCACGATCTGCCGCGACTTCTGCTTCAACGGCAACCTGATCATGCGTGCGACCGGCGA
CCGGATGCTGCTGTCGCCGCCGCTCGTGATTCGCGAGGCGGAAGTTGACGAGATCGTCGACAAGGCGAAGCGCGCGTTCG
ATGCGACGGCGGAGCGGGTGGGGTAG

Upstream 100 bases:

>100_bases
GACACCGAATACGAGGCGTTTTTCCGCGTGATCAGCTCGTGGGAACGCAGGCATCTGCTGCTGCACGTGTGAGCGTGCGG
CACTGTTATCCGGAGGAAAC

Downstream 100 bases:

>100_bases
TCGCGCGACACGTCGCCCAGCGTTCGTGCGGATGCCATTACGCGAGACGCATGTGGAATTTGTCCCGATCGGGATTTGAC
GCGCGTGACCGGACTGGCGA

Product: putative aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTDRNEAVPPHSTADYRALDAAHHIHPFSDMGALNRAGSRVIVKADGVYLWDSDGNKIIDGMAGLWCVSVGYGCKALADA
AYRQIQELPFYNTFFKTTHPPVIELSAMLAEVSPKGFNHFFYCNSGSEGNDTVLRLVHQYWRVQGQPQKKYVISRKNGYH
GSTIAGGTLGGMGYMHEQMPSKVENIVHIDQPYFFGEAAAGETPEAFGLARAQQLEAKILELGAENVAAFIGEPFQGAGG
VIFPPSTYWPEIQRICRKYDILLAADEVIGGFGRTGEWFAHQHFGFEPDLITMAKGLTSGYVPMGAVGIHERVARPIIDN
GEFNHGLTYSGHPVAAAVAVANLKLLRDEGIVERVKNDIGPYFQRRLRDALGDHPIVGEIAGAGLVAGVQLARDRDRRER
FGASVDIGTICRDFCFNGNLIMRATGDRMLLSPPLVIREAEVDEIVDKAKRAFDATAERVG

Sequences:

>Translated_461_residues
MTDRNEAVPPHSTADYRALDAAHHIHPFSDMGALNRAGSRVIVKADGVYLWDSDGNKIIDGMAGLWCVSVGYGCKALADA
AYRQIQELPFYNTFFKTTHPPVIELSAMLAEVSPKGFNHFFYCNSGSEGNDTVLRLVHQYWRVQGQPQKKYVISRKNGYH
GSTIAGGTLGGMGYMHEQMPSKVENIVHIDQPYFFGEAAAGETPEAFGLARAQQLEAKILELGAENVAAFIGEPFQGAGG
VIFPPSTYWPEIQRICRKYDILLAADEVIGGFGRTGEWFAHQHFGFEPDLITMAKGLTSGYVPMGAVGIHERVARPIIDN
GEFNHGLTYSGHPVAAAVAVANLKLLRDEGIVERVKNDIGPYFQRRLRDALGDHPIVGEIAGAGLVAGVQLARDRDRRER
FGASVDIGTICRDFCFNGNLIMRATGDRMLLSPPLVIREAEVDEIVDKAKRAFDATAERVG
>Mature_460_residues
TDRNEAVPPHSTADYRALDAAHHIHPFSDMGALNRAGSRVIVKADGVYLWDSDGNKIIDGMAGLWCVSVGYGCKALADAA
YRQIQELPFYNTFFKTTHPPVIELSAMLAEVSPKGFNHFFYCNSGSEGNDTVLRLVHQYWRVQGQPQKKYVISRKNGYHG
STIAGGTLGGMGYMHEQMPSKVENIVHIDQPYFFGEAAAGETPEAFGLARAQQLEAKILELGAENVAAFIGEPFQGAGGV
IFPPSTYWPEIQRICRKYDILLAADEVIGGFGRTGEWFAHQHFGFEPDLITMAKGLTSGYVPMGAVGIHERVARPIIDNG
EFNHGLTYSGHPVAAAVAVANLKLLRDEGIVERVKNDIGPYFQRRLRDALGDHPIVGEIAGAGLVAGVQLARDRDRRERF
GASVDIGTICRDFCFNGNLIMRATGDRMLLSPPLVIREAEVDEIVDKAKRAFDATAERVG

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=450, Percent_Identity=27.1111111111111, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4557809, Length=442, Percent_Identity=26.4705882352941, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI226442705, Length=451, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI24119277, Length=370, Percent_Identity=29.7297297297297, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI13994255, Length=434, Percent_Identity=26.036866359447, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI226442709, Length=422, Percent_Identity=27.0142180094787, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI284507298, Length=231, Percent_Identity=32.034632034632, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786991, Length=451, Percent_Identity=28.6031042128603, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1788044, Length=406, Percent_Identity=28.3251231527094, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1789759, Length=406, Percent_Identity=27.0935960591133, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI145693181, Length=407, Percent_Identity=25.0614250614251, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789016, Length=424, Percent_Identity=24.5283018867925, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787560, Length=358, Percent_Identity=29.3296089385475, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786349, Length=314, Percent_Identity=24.5222929936306, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71992977, Length=422, Percent_Identity=27.7251184834123, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32564660, Length=435, Percent_Identity=30.8045977011494, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25144271, Length=413, Percent_Identity=26.8765133171913, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25144274, Length=321, Percent_Identity=29.2834890965732, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=447, Percent_Identity=23.7136465324385, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=453, Percent_Identity=26.2693156732892, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324432, Length=434, Percent_Identity=25.8064516129032, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324386, Length=475, Percent_Identity=26.7368421052632, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321456, Length=371, Percent_Identity=24.5283018867925, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161085790, Length=431, Percent_Identity=28.7703016241299, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI28574759, Length=434, Percent_Identity=29.0322580645161, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21357415, Length=442, Percent_Identity=26.9230769230769, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21356575, Length=371, Percent_Identity=28.3018867924528, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281366494, Length=450, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24667139, Length=450, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24667143, Length=450, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 50479; Mature: 50348

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRNEAVPPHSTADYRALDAAHHIHPFSDMGALNRAGSRVIVKADGVYLWDSDGNKIID
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCEEEECCCCCCHHH
GMAGLWCVSVGYGCKALADAAYRQIQELPFYNTFFKTTHPPVIELSAMLAEVSPKGFNHF
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCEE
FYCNSGSEGNDTVLRLVHQYWRVQGQPQKKYVISRKNGYHGSTIAGGTLGGMGYMHEQMP
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
SKVENIVHIDQPYFFGEAAAGETPEAFGLARAQQLEAKILELGAENVAAFIGEPFQGAGG
HHHHHEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
VIFPPSTYWPEIQRICRKYDILLAADEVIGGFGRTGEWFAHQHFGFEPDLITMAKGLTSG
EEECCCCCCHHHHHHHHHHHHEEEEHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCC
YVPMGAVGIHERVARPIIDNGEFNHGLTYSGHPVAAAVAVANLKLLRDEGIVERVKNDIG
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYFQRRLRDALGDHPIVGEIAGAGLVAGVQLARDRDRRERFGASVDIGTICRDFCFNGNL
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCE
IMRATGDRMLLSPPLVIREAEVDEIVDKAKRAFDATAERVG
EEEECCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDRNEAVPPHSTADYRALDAAHHIHPFSDMGALNRAGSRVIVKADGVYLWDSDGNKIID
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEEEEEECCEEEECCCCCCHHH
GMAGLWCVSVGYGCKALADAAYRQIQELPFYNTFFKTTHPPVIELSAMLAEVSPKGFNHF
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCEE
FYCNSGSEGNDTVLRLVHQYWRVQGQPQKKYVISRKNGYHGSTIAGGTLGGMGYMHEQMP
EEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
SKVENIVHIDQPYFFGEAAAGETPEAFGLARAQQLEAKILELGAENVAAFIGEPFQGAGG
HHHHHEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
VIFPPSTYWPEIQRICRKYDILLAADEVIGGFGRTGEWFAHQHFGFEPDLITMAKGLTSG
EEECCCCCCHHHHHHHHHHHHEEEEHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHCCCC
YVPMGAVGIHERVARPIIDNGEFNHGLTYSGHPVAAAVAVANLKLLRDEGIVERVKNDIG
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYFQRRLRDALGDHPIVGEIAGAGLVAGVQLARDRDRRERFGASVDIGTICRDFCFNGNL
HHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCE
IMRATGDRMLLSPPLVIREAEVDEIVDKAKRAFDATAERVG
EEEECCCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]