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The map label for this gene is iscA [H]

Identifier: 78066899

GI number: 78066899

Start: 2499939

End: 2500262

Strand: Reverse

Name: iscA [H]

Synonym: Bcep18194_A5430

Alternate gene names: 78066899

Gene position: 2500262-2499939 (Counterclockwise)

Preceding gene: 78066900

Following gene: 161702957

Centisome position: 67.68

GC content: 60.49

Gene sequence:

>324_bases
ATGGCAATTTCACTGACCGAAAAAGCAGCACAGCACGTCCAGAAATACCTCGTCCGTCGCGGCAAGGGTGTGGGCCTGCG
GCTTGGCGTTCGCACGACCGGGTGCTCGGGGCTCGCGTACAAGCTCGAGTATGTCGACGAGCTGGCTCCCGAGGATCAGG
TGTTCGAGAGCCATGGCGTGAAGGTCATCGTCGATCCGAAGAGCCTGGCCTACATCGACGGCACCGAACTCGACTTCGCA
CGCGAAGGCCTGAACGAAGGGTTCAAGTTCAACAACCCGAACGTGAAGGACGAGTGCGGCTGCGGCGAATCGTTCCGCGT
GTGA

Upstream 100 bases:

>100_bases
ACGACACCACGGAAGGCGATCAGGCAGCAGCCTGAGCGGCATCGAGCGGCTTTCAGAAGGAACGCGGCGGGCCCCGGTGG
CGCGCCGCGGCAAGGACATC

Downstream 100 bases:

>100_bases
CCACGCGGATCTCCGCGCGATGCGGGCAAGAGGCGGCACGGGCCGCCTTTTTAATGTGCGGCGTTTGCCGCGCGACGAAC
CGGAATTGAACGCGACGATG

Product: Iron-sulphur cluster assembly protein IscA

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAISLTEKAAQHVQKYLVRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVIVDPKSLAYIDGTELDFA
REGLNEGFKFNNPNVKDECGCGESFRV

Sequences:

>Translated_107_residues
MAISLTEKAAQHVQKYLVRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVIVDPKSLAYIDGTELDFA
REGLNEGFKFNNPNVKDECGCGESFRV
>Mature_106_residues
AISLTEKAAQHVQKYLVRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGVKVIVDPKSLAYIDGTELDFAR
EGLNEGFKFNNPNVKDECGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=40.5660377358491, Blast_Score=95, Evalue=2e-20,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=42.1686746987952, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=70.0934579439252, Blast_Score=162, Evalue=4e-42,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=48.5714285714286, Blast_Score=112, Evalue=5e-27,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=36.3636363636364, Blast_Score=71, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=38.6792452830189, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=38.6792452830189, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=42.8571428571429, Blast_Score=92, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24642263, Length=104, Percent_Identity=43.2692307692308, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11782; Mature: 11650

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISLTEKAAQHVQKYLVRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGV
CCCCCHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
KVIVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AISLTEKAAQHVQKYLVRRGKGVGLRLGVRTTGCSGLAYKLEYVDELAPEDQVFESHGV
CCCCHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
KVIVDPKSLAYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]