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The map label for this gene is sdhA [C]

Identifier: 78066756

GI number: 78066756

Start: 2341989

End: 2343728

Strand: Direct

Name: sdhA [C]

Synonym: Bcep18194_A5287

Alternate gene names: 78066756

Gene position: 2341989-2343728 (Clockwise)

Preceding gene: 78066755

Following gene: 78066757

Centisome position: 63.4

GC content: 65.92

Gene sequence:

>1740_bases
ATGACTACCCATGTGCTCGAATACGACATCGTCGTGGTCGGCGGCGGCACCGCCGGCCCGATGGCGGCGATCAAGGCAAA
GGAGCGCGATCCGTCCCTGCGCGTGCTGCTGCTCGAAAAAGCCAACGTGAAGCGCAGCGGCGCGATCTCGATGGGGATGG
ACGGCCTGAACAACGCGGTGATCCCCGGGCACGCGACGCCCGAGCAGTACACGCGCGAGATCACGATCGCCAACGACGGG
ATCGTCGACCAGGAAGCCGTGTACGCGTATGCGGCGCACAGCTTCGCGACGATCGAGCAACTCGACCGCTGGGGCGTGAA
GTTCGAGAAGGACGGCACCGGCGACTACGCGGTGAAGAAGGTTCATCACATGGGCTCGTACGTGCTGCCGATGCCGGAAG
GACACGACATCAAGAAGGTGCTGTACCGGCAGCTGAAGCGCGCACGCATCGCGATCACGAACCGGATCGTCGCGACGCGC
GTGCTGACCGACGCGCAAGGCCGCGCGAGCGGCGTGCTCGGCTTCGACTGCCGCACCGCCGAATTCCACGTGATCCGCGC
GAAGGCCGTGATTCTCTGCTGCGGCGCGGCCGGCCGCCTCGGGCTACCGGCCTCGGGTTACCTGATGGGCACGTACGAGA
ACCCGACCAACGCAGGCGACGGTTATGCAATGGCGTATCACGCCGGTGCCGCGCTCGCGAATCTCGAATGCTTCCAGATC
AACCCGCTGATCAAGGACTACAACGGCCCCGCGTGCGCGTACGTCACCGGCCCGCTCGGCGGCTTCACCGCGAACGGCAA
GGGCGAACGCTTCATCGAATGCGATTACTGGAGCGGCCAGATGATGTGGGAGTTCTACCAGGAACTGCAAAGCGGCAACG
GCCCCGTGTTCCTGAAGCTCGACCATCTCGCGGAAGAAACCATCCAGACGATCGAGCAGATCCTCCATACGAACGAGCGG
CCGAGCCGTGGCCGCTTCCATGCGGGGCGCGGCACCGACTACCGCAGCCAGATGATCGAGATGCACATCTCCGAGATCGG
TTTCTGCAGCGGCCACAGCGCATCGGGCGTGTACGTGAACGCACGCGCGGAGACGACGGTGCCCGGGCTCTACGCGGCCG
GCGACATGGCGGCCGTCCCGCACAACTACATGCTCGGCGCATTCACGTACGGCTGGTTTGCCGGGCAGAATGCCGCCGAC
TACGTGGCCGGCCGAGAGCATGCGCCGGTGGACGACGAACAGGTCGCCGCCGAACGCACGCGCGTGCTCGCGCCGCTGCT
GCGCGAGCGCGGCCTGGCACCAGCACAGGTCGAATACAAGCTGCGCCGCATGGTGAACGACTATCTGCAACCGCCGAAGG
TGACGCGCAAGATGGAAATCGGGCTGCAACGCTTCGACGCCATTACTGAGGATATCGAAGAGATCAAGGCGAGCCACCCG
CACGAACTGATGCGCGCCGCCGAAGTGCGTGCGATTCGCGATTGCGCGGAAATGGCCGCGCGCGCGTCGCTGTTCCGCAC
CGAGAGCCGCTGGGGGCTGTACCACCATCGCGTCGACTTCCCGCATCGCAACGACGCCGAATGGTTCTGCCACACCTGGC
TGCGCAAGGACGCCGACGGTGCGATGCGCAGCGAGAAGCGGCCGGTCGAACCGTACCTCGTGCCGCTTGCCGACGACGAT
CGCGCCGCCTACACGAATCTGCGCATCCGCGAACCGGCCGCGCTCGCGGCCGCCCTTTGA

Upstream 100 bases:

>100_bases
CACCGATCGACTACGAATACCTCTACTTCCGCGGCGACGCCTTCCAGTACCGGCTGCGCATCGACCGGCAGAAGCCGCGC
AAACCTGCAAGGAAACAGCG

Downstream 100 bases:

>100_bases
GGAACCCGCCATGTCCCATACCTCGCACGACATCTTCCAGCGCAGCAGCGCACCCGTCACGATCGACGAGACCCTGTGCA
TCGCCGACAAGGGCTGCACC

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MTTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVYAYAAHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDAQGRASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIQTIEQILHTNER
PSRGRFHAGRGTDYRSQMIEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGREHAPVDDEQVAAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAITEDIEEIKASHP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDADGAMRSEKRPVEPYLVPLADDD
RAAYTNLRIREPAALAAAL

Sequences:

>Translated_579_residues
MTTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDG
IVDQEAVYAYAAHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATR
VLTDAQGRASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIQTIEQILHTNER
PSRGRFHAGRGTDYRSQMIEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAAD
YVAGREHAPVDDEQVAAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAITEDIEEIKASHP
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDADGAMRSEKRPVEPYLVPLADDD
RAAYTNLRIREPAALAAAL
>Mature_578_residues
TTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAVIPGHATPEQYTREITIANDGI
VDQEAVYAYAAHSFATIEQLDRWGVKFEKDGTGDYAVKKVHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRV
LTDAQGRASGVLGFDCRTAEFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQIN
PLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKLDHLAEETIQTIEQILHTNERP
SRGRFHAGRGTDYRSQMIEMHISEIGFCSGHSASGVYVNARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADY
VAGREHAPVDDEQVAAERTRVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAITEDIEEIKASHPH
ELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDADGAMRSEKRPVEPYLVPLADDDR
AAYTNLRIREPAALAAAL

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=459, Percent_Identity=25.0544662309368, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790597, Length=605, Percent_Identity=21.9834710743802, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17505833, Length=539, Percent_Identity=22.6345083487941, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64093; Mature: 63961

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCCEEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVYAYAAHSFATIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDAQGRASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCCEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIQTIEQILHTNERPSRGRFHAGRGTDYRSQMIEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGREHAPVDDEQVAAERT
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
RVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAITEDIEEIKASHP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDADG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
AMRSEKRPVEPYLVPLADDDRAAYTNLRIREPAALAAAL
HHHCCCCCCCCEEEEECCCCCCCCCCEEEECCHHHHHCC
>Mature Secondary Structure 
TTHVLEYDIVVVGGGTAGPMAAIKAKERDPSLRVLLLEKANVKRSGAISMGMDGLNNAV
CCEEEEEEEEEECCCCCCCHHHEECCCCCCCEEEEEEECCCCCCCCCEEECCCCCCCCC
IPGHATPEQYTREITIANDGIVDQEAVYAYAAHSFATIEQLDRWGVKFEKDGTGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHDIKKVLYRQLKRARIAITNRIVATRVLTDAQGRASGVLGFDCRTA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCC
EFHVIRAKAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHCCEEEEE
NPLIKDYNGPACAYVTGPLGGFTANGKGERFIECDYWSGQMMWEFYQELQSGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
DHLAEETIQTIEQILHTNERPSRGRFHAGRGTDYRSQMIEMHISEIGFCSGHSASGVYVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEE
ARAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGQNAADYVAGREHAPVDDEQVAAERT
CCCCCCCCCEEECCCCCCCCCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHH
RVLAPLLRERGLAPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAITEDIEEIKASHP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HELMRAAEVRAIRDCAEMAARASLFRTESRWGLYHHRVDFPHRNDAEWFCHTWLRKDADG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
AMRSEKRPVEPYLVPLADDDRAAYTNLRIREPAALAAAL
HHHCCCCCCCCEEEEECCCCCCCCCCEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]