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The map label for this gene is emrB [H]

Identifier: 78066437

GI number: 78066437

Start: 1993755

End: 1995344

Strand: Direct

Name: emrB [H]

Synonym: Bcep18194_A4968

Alternate gene names: 78066437

Gene position: 1993755-1995344 (Clockwise)

Preceding gene: 78066434

Following gene: 78066439

Centisome position: 53.97

GC content: 59.06

Gene sequence:

>1590_bases
ATGAGTACCATGCTCGTCGCCGGCGGTGGTACGCCGGAAGCCTCTCCCGTTCCAGGCGCCACCGTGCCGCCATTTCGTTC
GATCGCTGCGATTGCTGCCGTGCTGTTCGGGGCAGTGATATCGACACTGACATCGCGCATCACATCGCTCGGATTGGCGG
ATTTACGCGGGGCACTCGGTATCGGCTTCGACGAAGGGGCGTGGATCAACACGGCGTTCATTGCGTCACAGATGTTCATA
GGTCCGCTAGCGATCGCAGCCGCGTTCTTGCTCGGCACACGGCGCGTGCTTCTTGCAGGAGCCGTTGTATTCCTTGTCGC
CGAGAGCGTATTGCCGCTTTGTCCGGGATTCGGCTCACTCATCATCTGCCAGAGCGTTGCCGGGCTTGCATCCGGCGTGT
TCGTACCGCTGACCGTTGGCTTCATCGTACGCACATTGCCGTCGCGTTTCATTCCATTCGGCATCGCCGCGTATGCGATG
AACCTCGAGATGTCATTGAACTTGTCAGCTACCCTCGAAGGTTGGTATAGCGAGCACCTGAACTGGCGCTGGCTATTCTG
GCAAAACGCACTGATGACCATCCCGTTCATCGTCTGCCTGCTTTTGTCATTGTCCAATGAGCCGATCAAGCGCTTCGCGT
CTGGTGCCGACTACCGGGGCATGCTACTCGGTGCAAGTGGATTTACCTGTCTGTGCATCGCACTCGACCAGGGAGAGCGC
CTGTTTTGGCTGGAGTCGCCGCTGATCGTTACGCTACTTTGCATCAGTATCGTGACGATCTCCACCTTTCTTGTCCACGA
ACTCGTTTCGAAGCAAGCGGGCCTCAATCTGGGTTACCTGGTCCGGCCGAACGTACTGCTGCTCATGCTGCTCGTCGGTC
TCGTGCGCTTTACCGTTCTCAACACCAGCTTCATTCCGTCGCTCTTTCTTGCGAGTACGTATGGACTCCGGCCGCTGCAG
ATCGGCGATACGCTGCGCTGGATCGCGATGCCGCAGTTCCTGTTTGCGCCGTGTGTCGCATTGTTGCTGCAACGGTTCGA
CTCGCGTCGGCTGATCGTGATCGGCTTCGTGATGGTCGCGATTGCTTTCGCGCTTGGATCGCGTTTGACTTCCGTATGGG
CCGAACCTGACTTCATTCCGTCTCAGTTGCTTCAGGCGCTGGGCCAGACAATGGCGCTTACGTCGGTCATATTTTTCTTC
GGAAAGCACGTCACTGCTGAGCACGCGCTGACATTCGGCGCCGTCGTGCAGACGACGCGTCTGCTCGGTGGGCAGCTTGG
TACAACGTCGATCGCTGTAGTCCAACGCATCATGGAGGCAACACATTCCAATCGCGTCGGCCTGCATGTGGCATTGTCCG
ATCCGGTGACGCTCGAGCGCCTGCGCGTTGGCGCCGCGTCGCTCGTCACGCATGGCGCGACATTCGCAACTGGAGACAGT
GGTACGTATGTGCTCCTTGATCGAGCGGTCCGCGTACAGGCGACGACGCTCGCGCTTGCGGACAATTTCCGCGTGGCAAT
GACGTGCGCGATGGTTGGCGCATTGATCGGAATGATGCTCAGGCCGGTGCGAGCGCCCCGATCGTCGTGA

Upstream 100 bases:

>100_bases
TGGGGCGTCTGCGTCACCATCTGTTTTCTCCATCATGCAGGCAGGATTCAGGACGTCGCGGTATGCTTGCGCAAACGTCC
TGCGCGGGAGTTGTTCGACA

Downstream 100 bases:

>100_bases
GCGTCCAGTTTGGTGTGGTGTACTGACGGCGGTAGCACCCCCACTATGAAAAGGAACCGTCGGGAGCCGAGTTCCCGGGA
ATGTATTGGTGGTGAGGTCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSTMLVAGGGTPEASPVPGATVPPFRSIAAIAAVLFGAVISTLTSRITSLGLADLRGALGIGFDEGAWINTAFIASQMFI
GPLAIAAAFLLGTRRVLLAGAVVFLVAESVLPLCPGFGSLIICQSVAGLASGVFVPLTVGFIVRTLPSRFIPFGIAAYAM
NLEMSLNLSATLEGWYSEHLNWRWLFWQNALMTIPFIVCLLLSLSNEPIKRFASGADYRGMLLGASGFTCLCIALDQGER
LFWLESPLIVTLLCISIVTISTFLVHELVSKQAGLNLGYLVRPNVLLLMLLVGLVRFTVLNTSFIPSLFLASTYGLRPLQ
IGDTLRWIAMPQFLFAPCVALLLQRFDSRRLIVIGFVMVAIAFALGSRLTSVWAEPDFIPSQLLQALGQTMALTSVIFFF
GKHVTAEHALTFGAVVQTTRLLGGQLGTTSIAVVQRIMEATHSNRVGLHVALSDPVTLERLRVGAASLVTHGATFATGDS
GTYVLLDRAVRVQATTLALADNFRVAMTCAMVGALIGMMLRPVRAPRSS

Sequences:

>Translated_529_residues
MSTMLVAGGGTPEASPVPGATVPPFRSIAAIAAVLFGAVISTLTSRITSLGLADLRGALGIGFDEGAWINTAFIASQMFI
GPLAIAAAFLLGTRRVLLAGAVVFLVAESVLPLCPGFGSLIICQSVAGLASGVFVPLTVGFIVRTLPSRFIPFGIAAYAM
NLEMSLNLSATLEGWYSEHLNWRWLFWQNALMTIPFIVCLLLSLSNEPIKRFASGADYRGMLLGASGFTCLCIALDQGER
LFWLESPLIVTLLCISIVTISTFLVHELVSKQAGLNLGYLVRPNVLLLMLLVGLVRFTVLNTSFIPSLFLASTYGLRPLQ
IGDTLRWIAMPQFLFAPCVALLLQRFDSRRLIVIGFVMVAIAFALGSRLTSVWAEPDFIPSQLLQALGQTMALTSVIFFF
GKHVTAEHALTFGAVVQTTRLLGGQLGTTSIAVVQRIMEATHSNRVGLHVALSDPVTLERLRVGAASLVTHGATFATGDS
GTYVLLDRAVRVQATTLALADNFRVAMTCAMVGALIGMMLRPVRAPRSS
>Mature_528_residues
STMLVAGGGTPEASPVPGATVPPFRSIAAIAAVLFGAVISTLTSRITSLGLADLRGALGIGFDEGAWINTAFIASQMFIG
PLAIAAAFLLGTRRVLLAGAVVFLVAESVLPLCPGFGSLIICQSVAGLASGVFVPLTVGFIVRTLPSRFIPFGIAAYAMN
LEMSLNLSATLEGWYSEHLNWRWLFWQNALMTIPFIVCLLLSLSNEPIKRFASGADYRGMLLGASGFTCLCIALDQGERL
FWLESPLIVTLLCISIVTISTFLVHELVSKQAGLNLGYLVRPNVLLLMLLVGLVRFTVLNTSFIPSLFLASTYGLRPLQI
GDTLRWIAMPQFLFAPCVALLLQRFDSRRLIVIGFVMVAIAFALGSRLTSVWAEPDFIPSQLLQALGQTMALTSVIFFFG
KHVTAEHALTFGAVVQTTRLLGGQLGTTSIAVVQRIMEATHSNRVGLHVALSDPVTLERLRVGAASLVTHGATFATGDSG
TYVLLDRAVRVQATTLALADNFRVAMTCAMVGALIGMMLRPVRAPRSS

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=424, Percent_Identity=21.6981132075472, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788710, Length=345, Percent_Identity=23.768115942029, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56536; Mature: 56405

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMLVAGGGTPEASPVPGATVPPFRSIAAIAAVLFGAVISTLTSRITSLGLADLRGALG
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
IGFDEGAWINTAFIASQMFIGPLAIAAAFLLGTRRVLLAGAVVFLVAESVLPLCPGFGSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IICQSVAGLASGVFVPLTVGFIVRTLPSRFIPFGIAAYAMNLEMSLNLSATLEGWYSEHL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEEEECCHHHHHHHHHHCC
NWRWLFWQNALMTIPFIVCLLLSLSNEPIKRFASGADYRGMLLGASGFTCLCIALDQGER
CEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCE
LFWLESPLIVTLLCISIVTISTFLVHELVSKQAGLNLGYLVRPNVLLLMLLVGLVRFTVL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHH
NTSFIPSLFLASTYGLRPLQIGDTLRWIAMPQFLFAPCVALLLQRFDSRRLIVIGFVMVA
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IAFALGSRLTSVWAEPDFIPSQLLQALGQTMALTSVIFFFGKHVTAEHALTFGAVVQTTR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLGGQLGTTSIAVVQRIMEATHSNRVGLHVALSDPVTLERLRVGAASLVTHGATFATGDS
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCC
GTYVLLDRAVRVQATTLALADNFRVAMTCAMVGALIGMMLRPVRAPRSS
CCEEEEECCHHEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STMLVAGGGTPEASPVPGATVPPFRSIAAIAAVLFGAVISTLTSRITSLGLADLRGALG
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
IGFDEGAWINTAFIASQMFIGPLAIAAAFLLGTRRVLLAGAVVFLVAESVLPLCPGFGSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
IICQSVAGLASGVFVPLTVGFIVRTLPSRFIPFGIAAYAMNLEMSLNLSATLEGWYSEHL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEEEECCHHHHHHHHHHCC
NWRWLFWQNALMTIPFIVCLLLSLSNEPIKRFASGADYRGMLLGASGFTCLCIALDQGER
CEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCE
LFWLESPLIVTLLCISIVTISTFLVHELVSKQAGLNLGYLVRPNVLLLMLLVGLVRFTVL
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHH
NTSFIPSLFLASTYGLRPLQIGDTLRWIAMPQFLFAPCVALLLQRFDSRRLIVIGFVMVA
HHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IAFALGSRLTSVWAEPDFIPSQLLQALGQTMALTSVIFFFGKHVTAEHALTFGAVVQTTR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LLGGQLGTTSIAVVQRIMEATHSNRVGLHVALSDPVTLERLRVGAASLVTHGATFATGDS
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCC
GTYVLLDRAVRVQATTLALADNFRVAMTCAMVGALIGMMLRPVRAPRSS
CCEEEEECCHHEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]