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The map label for this gene is nemA [H]

Identifier: 78066422

GI number: 78066422

Start: 1975554

End: 1976672

Strand: Reverse

Name: nemA [H]

Synonym: Bcep18194_A4953

Alternate gene names: 78066422

Gene position: 1976672-1975554 (Counterclockwise)

Preceding gene: 78066423

Following gene: 78066420

Centisome position: 53.51

GC content: 61.13

Gene sequence:

>1119_bases
ATGTCCTCCCTGCTCAGCAACTTCGATCTGAACGGTCTTATCCTGCCGAATCGCGTCGTGATGGGGCCAATGACCCGGTC
CCGCGCACCGTATCGCGGCCAGCCAACCGAACTCATGGCCGAATACTACGCACAGCGCGCGTCAGCCGGGCTTATCGTGT
CCGAAGCGACCAACGTAAGCCCGGTCTCCGCATCGTTCGAACTCACGCCGGGCCTCATTACCGATGAACAGGTAGCCGGC
TGGAAAAAAGTCACCGAGGCCGTCCATGCGAAAGGCGGACGCATCTTCGCGCAGCTCTGGCACGGCGGACGTGTCAGTTC
GCTGACGCTACTCGGCGGGCAAGCGCCATTGTCGCCATCCGGCGTGAACGACGATCTCGAACAGTTGCAGGTGTGGGCCC
AATTGCAAAACGGGTATTACACGAAGATCCATGCGACACCGTCACGCGCGATGACGACTGACGAAGTGGTCAAGACGATC
GATGAATTCCGGCAGTCCGCTGCTCACGCGAGGTCGGCCGGTTTTGATGGTGTCGAGATCCACGCGGCTAACGGCTACCT
GCCGCATCAGTTCCTGTCGTCGACGCTGAACCGCCGTGACGACCGCTACGGTGGCACGCTGGCGAACCGCGTGCGCTTTC
TCGAGGAGATCATCGCCGCGGTGGGTGGCGAGATTCCGTTGAGCCGTGTCGGTGTGCGCATCTCGCCATACGCGAAATAC
AACAATGTGCGCGATACGGACCCGGACGCGACATATGCCTATGTCGGCCGCATGCTCGACGAAGCCGGTGTCGCGTATGT
GCATGCTGCGGATACCAATGGCTGGAGCGGTGAACCCGACCTGCCACGTATCATCGAGATCGTGCGCAAGAGCTTCGGAG
GGGCATTAATCGTAAACGGCGGCATTTCGCCAGATGTCGCAACCAAATTGATCATGTCAGGCGACGCCGATCTTGTCGCG
TTCGCACGTGCATACATCGCCAATCCGGATCTGGTCGAACGCATCGCGAACAAGGCTCCGCTCGCCGCACCGCAGGCGGT
GGGCTGGTATGGCGGCGATCGGACCGGTTACGTCGACTATGGGCGTCACGACGCAACGGGACACACCACCGGCGAGTGA

Upstream 100 bases:

>100_bases
ACGCTTCCCATAAACCTTCCGCAGGGTTGACGGCTCCCGCCGTTCACCGCCGCAACCGACACTGGCGGTATGGCCCTGCT
CGTTCGAAAGGAATACCACC

Downstream 100 bases:

>100_bases
CGGCTACGCGCCTGTCGTACAAGGCGATATCGTGAAACGGACGAGGAGATGGCTCGACGACACAACGCCGAGCCAACCGC
AGATTGAAAACAGCAGAGCA

Product: NADH-flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSSLLSNFDLNGLILPNRVVMGPMTRSRAPYRGQPTELMAEYYAQRASAGLIVSEATNVSPVSASFELTPGLITDEQVAG
WKKVTEAVHAKGGRIFAQLWHGGRVSSLTLLGGQAPLSPSGVNDDLEQLQVWAQLQNGYYTKIHATPSRAMTTDEVVKTI
DEFRQSAAHARSAGFDGVEIHAANGYLPHQFLSSTLNRRDDRYGGTLANRVRFLEEIIAAVGGEIPLSRVGVRISPYAKY
NNVRDTDPDATYAYVGRMLDEAGVAYVHAADTNGWSGEPDLPRIIEIVRKSFGGALIVNGGISPDVATKLIMSGDADLVA
FARAYIANPDLVERIANKAPLAAPQAVGWYGGDRTGYVDYGRHDATGHTTGE

Sequences:

>Translated_372_residues
MSSLLSNFDLNGLILPNRVVMGPMTRSRAPYRGQPTELMAEYYAQRASAGLIVSEATNVSPVSASFELTPGLITDEQVAG
WKKVTEAVHAKGGRIFAQLWHGGRVSSLTLLGGQAPLSPSGVNDDLEQLQVWAQLQNGYYTKIHATPSRAMTTDEVVKTI
DEFRQSAAHARSAGFDGVEIHAANGYLPHQFLSSTLNRRDDRYGGTLANRVRFLEEIIAAVGGEIPLSRVGVRISPYAKY
NNVRDTDPDATYAYVGRMLDEAGVAYVHAADTNGWSGEPDLPRIIEIVRKSFGGALIVNGGISPDVATKLIMSGDADLVA
FARAYIANPDLVERIANKAPLAAPQAVGWYGGDRTGYVDYGRHDATGHTTGE
>Mature_371_residues
SSLLSNFDLNGLILPNRVVMGPMTRSRAPYRGQPTELMAEYYAQRASAGLIVSEATNVSPVSASFELTPGLITDEQVAGW
KKVTEAVHAKGGRIFAQLWHGGRVSSLTLLGGQAPLSPSGVNDDLEQLQVWAQLQNGYYTKIHATPSRAMTTDEVVKTID
EFRQSAAHARSAGFDGVEIHAANGYLPHQFLSSTLNRRDDRYGGTLANRVRFLEEIIAAVGGEIPLSRVGVRISPYAKYN
NVRDTDPDATYAYVGRMLDEAGVAYVHAADTNGWSGEPDLPRIIEIVRKSFGGALIVNGGISPDVATKLIMSGDADLVAF
ARAYIANPDLVERIANKAPLAAPQAVGWYGGDRTGYVDYGRHDATGHTTGE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=42.0765027322404, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1789463, Length=350, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=369, Percent_Identity=27.6422764227642, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=230, Percent_Identity=29.1304347826087, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=201, Percent_Identity=31.3432835820896, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17559804, Length=210, Percent_Identity=30.952380952381, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=356, Percent_Identity=25.561797752809, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=366, Percent_Identity=24.3169398907104, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=382, Percent_Identity=32.4607329842932, Blast_Score=166, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=31.3984168865435, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40075; Mature: 39944

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLSNFDLNGLILPNRVVMGPMTRSRAPYRGQPTELMAEYYAQRASAGLIVSEATNVS
CCCHHHCCCCCCEECCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCC
PVSASFELTPGLITDEQVAGWKKVTEAVHAKGGRIFAQLWHGGRVSSLTLLGGQAPLSPS
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCEEEEEEECCCCCCCCC
GVNDDLEQLQVWAQLQNGYYTKIHATPSRAMTTDEVVKTIDEFRQSAAHARSAGFDGVEI
CCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLPHQFLSSTLNRRDDRYGGTLANRVRFLEEIIAAVGGEIPLSRVGVRISPYAKY
EECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEEECCHHHC
NNVRDTDPDATYAYVGRMLDEAGVAYVHAADTNGWSGEPDLPRIIEIVRKSFGGALIVNG
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECC
GISPDVATKLIMSGDADLVAFARAYIANPDLVERIANKAPLAAPQAVGWYGGDRTGYVDY
CCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEC
GRHDATGHTTGE
CCCCCCCCCCCC
>Mature Secondary Structure 
SSLLSNFDLNGLILPNRVVMGPMTRSRAPYRGQPTELMAEYYAQRASAGLIVSEATNVS
CCHHHCCCCCCEECCCCEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCC
PVSASFELTPGLITDEQVAGWKKVTEAVHAKGGRIFAQLWHGGRVSSLTLLGGQAPLSPS
CCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCEEEEEEECCCCCCCCC
GVNDDLEQLQVWAQLQNGYYTKIHATPSRAMTTDEVVKTIDEFRQSAAHARSAGFDGVEI
CCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HAANGYLPHQFLSSTLNRRDDRYGGTLANRVRFLEEIIAAVGGEIPLSRVGVRISPYAKY
EECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCEEECCHHHC
NNVRDTDPDATYAYVGRMLDEAGVAYVHAADTNGWSGEPDLPRIIEIVRKSFGGALIVNG
CCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECC
GISPDVATKLIMSGDADLVAFARAYIANPDLVERIANKAPLAAPQAVGWYGGDRTGYVDY
CCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEC
GRHDATGHTTGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]