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The map label for this gene is cobB [H]

Identifier: 78066269

GI number: 78066269

Start: 1813054

End: 1814367

Strand: Reverse

Name: cobB [H]

Synonym: Bcep18194_A4799

Alternate gene names: 78066269

Gene position: 1814367-1813054 (Counterclockwise)

Preceding gene: 78066270

Following gene: 78066266

Centisome position: 49.11

GC content: 68.95

Gene sequence:

>1314_bases
ATGCTCGCCTGCCCCGCGCTGTTCGTCAGTGCGCCCGCGTCGGGCCAGGGCAAGACGTCGGTGACGGCGGGCCTCGCGCG
GCTGCACCGCCGGCTCGGCCGGCGCGTGCGCGTGTTCAAGACCGGGCCCGATTTTCTCGATCCGATGCTGCTCGAACGCG
CGAGCGGCGCACCGGTGCATGCGCTCGATCTCGGGATGGTCGGCGAGGCCGGCTGCCGTGCGCTGCTGGCCGATGCCGCG
CGCGATGCCGACCTGATCCTGATCGAAGGCGTGATGGGGCTGTTCGACGGCACGCCGAGCAGTGCCGACCTCGCGGCCGC
GTTCGGCGTGCCGGTCGTCGCGGTGATTTCCGCGAAGTCGATGGCGCAGACGTTCGCGGCGATCGCGTTCGGGCTCGCGC
GGTTCCGGCCGGGGCTGCCGTTTCACGGTGTGCTGGCGAATCGCGTCGGCTCGGAGCGGCACGCGGAACTGCTGCAACAG
GCGCTGCCCGATGACCTGCGCTGGCTCGGGCACGTGCCGGCCGATGCGGGCATCGCTTTACCCGAAAGGCATCTTGGCCT
GCACCAGCCGAGCGACATCGACGATCTCGATGCGCGCCTCGATCGCGCGGCCGACGTGCTGGCGCAAACGGCGCTTGCCG
AGTTGCCGCCGGCCGTTGCGTTCGCGGAACCCGATGCAGCCGCGCCGCTGCCGCGTGCGCTCGCCGGCAAGCGGATCGCG
GTGGCGCGCGATGTGGCGTTCTCGTTCATCTATCCGGCGAATCTCGCGTTGCTCGAGACGCTCGGTGCGGAAGTGGGGTT
CTTCTCGCCGCTCGCCGACGAACCGGTGCCGGACGATTGCGACGCGCTGTTCCTTCCCGGTGGCTATCCGGAGTTGCACG
CGGCAACGCTTACGGGAAACGCCACGACCGCACGGTCGATTCGCGCACACGCGGCGGCTGGCCGGCCGATCGTCGCGGAA
TGCGGGGGGATGGTGTATCTGTGTGACGCGCTGACCGATGTGGACGGCGTGACGACGCCGATGCTTGGGCTGTTGCCGGG
TCAGGCGACGATGCAGCGCCGGTTCGCGGCGCTCGGCATGCAGCAGCTCGATACACGCAGCGGGACGATGCGTGGGCATA
CGTTCCACTATTCTCGGCTCGCGACGCCGCTGGAACCAGTCGCGCGCGCCGTACGCCCCGACAGTGCATCGGGCAGCGGT
GAAGCCGTCTATTGCCACGACGCGATAGTCGCGACCTACATGCATATGTACTGGCCGTCGAATCAGGATGTGGTCGTAGC
GTTGTTCAGCGGGGAAGGAAGCGTTCTAGGCTGA

Upstream 100 bases:

>100_bases
CGCTGATCGATGCGGCCGACCTCGTCACCGAAATGCGGCTTGTCAAGCATCCGTACAAGGAACAAGGCGTGAAGGCGCAG
CGCGGCGTGGAGTTCTGAGC

Downstream 100 bases:

>100_bases
CCGATGCCGGGCTCGGATCGGGAATGCGTCGCCCGCATGCGGGCCAGGAAGGCCTCGCTGTCGAATGGCTCGGGTGCTCC
CGATGCTCCCGATGCGTCAC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAA
RDADLILIEGVMGLFDGTPSSADLAAAFGVPVVAVISAKSMAQTFAAIAFGLARFRPGLPFHGVLANRVGSERHAELLQQ
ALPDDLRWLGHVPADAGIALPERHLGLHQPSDIDDLDARLDRAADVLAQTALAELPPAVAFAEPDAAAPLPRALAGKRIA
VARDVAFSFIYPANLALLETLGAEVGFFSPLADEPVPDDCDALFLPGGYPELHAATLTGNATTARSIRAHAAAGRPIVAE
CGGMVYLCDALTDVDGVTTPMLGLLPGQATMQRRFAALGMQQLDTRSGTMRGHTFHYSRLATPLEPVARAVRPDSASGSG
EAVYCHDAIVATYMHMYWPSNQDVVVALFSGEGSVLG

Sequences:

>Translated_437_residues
MLACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAA
RDADLILIEGVMGLFDGTPSSADLAAAFGVPVVAVISAKSMAQTFAAIAFGLARFRPGLPFHGVLANRVGSERHAELLQQ
ALPDDLRWLGHVPADAGIALPERHLGLHQPSDIDDLDARLDRAADVLAQTALAELPPAVAFAEPDAAAPLPRALAGKRIA
VARDVAFSFIYPANLALLETLGAEVGFFSPLADEPVPDDCDALFLPGGYPELHAATLTGNATTARSIRAHAAAGRPIVAE
CGGMVYLCDALTDVDGVTTPMLGLLPGQATMQRRFAALGMQQLDTRSGTMRGHTFHYSRLATPLEPVARAVRPDSASGSG
EAVYCHDAIVATYMHMYWPSNQDVVVALFSGEGSVLG
>Mature_437_residues
MLACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVHALDLGMVGEAGCRALLADAA
RDADLILIEGVMGLFDGTPSSADLAAAFGVPVVAVISAKSMAQTFAAIAFGLARFRPGLPFHGVLANRVGSERHAELLQQ
ALPDDLRWLGHVPADAGIALPERHLGLHQPSDIDDLDARLDRAADVLAQTALAELPPAVAFAEPDAAAPLPRALAGKRIA
VARDVAFSFIYPANLALLETLGAEVGFFSPLADEPVPDDCDALFLPGGYPELHAATLTGNATTARSIRAHAAAGRPIVAE
CGGMVYLCDALTDVDGVTTPMLGLLPGQATMQRRFAALGMQQLDTRSGTMRGHTFHYSRLATPLEPVARAVRPDSASGSG
EAVYCHDAIVATYMHMYWPSNQDVVVALFSGEGSVLG

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45743; Mature: 45743

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLADAARDADLILIEGVMGLFDGTPSSADLAAAFGVPVVAVISAKS
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
MAQTFAAIAFGLARFRPGLPFHGVLANRVGSERHAELLQQALPDDLRWLGHVPADAGIAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQPSDIDDLDARLDRAADVLAQTALAELPPAVAFAEPDAAAPLPRALAGKRIA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCEEE
VARDVAFSFIYPANLALLETLGAEVGFFSPLADEPVPDDCDALFLPGGYPELHAATLTGN
EHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECC
ATTARSIRAHAAAGRPIVAECGGMVYLCDALTDVDGVTTPMLGLLPGQATMQRRFAALGM
CHHHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
QQLDTRSGTMRGHTFHYSRLATPLEPVARAVRPDSASGSGEAVYCHDAIVATYMHMYWPS
HHHHCCCCCCCCCEEEHHHHCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCC
NQDVVVALFSGEGSVLG
CCCEEEEEECCCCCCCC
>Mature Secondary Structure
MLACPALFVSAPASGQGKTSVTAGLARLHRRLGRRVRVFKTGPDFLDPMLLERASGAPVH
CCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEE
ALDLGMVGEAGCRALLADAARDADLILIEGVMGLFDGTPSSADLAAAFGVPVVAVISAKS
EEECCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
MAQTFAAIAFGLARFRPGLPFHGVLANRVGSERHAELLQQALPDDLRWLGHVPADAGIAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCC
PERHLGLHQPSDIDDLDARLDRAADVLAQTALAELPPAVAFAEPDAAAPLPRALAGKRIA
CHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHCCCEEE
VARDVAFSFIYPANLALLETLGAEVGFFSPLADEPVPDDCDALFLPGGYPELHAATLTGN
EHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECC
ATTARSIRAHAAAGRPIVAECGGMVYLCDALTDVDGVTTPMLGLLPGQATMQRRFAALGM
CHHHHHHHHHHCCCCCHHHHCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHH
QQLDTRSGTMRGHTFHYSRLATPLEPVARAVRPDSASGSGEAVYCHDAIVATYMHMYWPS
HHHHCCCCCCCCCEEEHHHHCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHCCC
NQDVVVALFSGEGSVLG
CCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]