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The map label for this gene is rluB [H]

Identifier: 78066108

GI number: 78066108

Start: 1619405

End: 1621171

Strand: Direct

Name: rluB [H]

Synonym: Bcep18194_A4638

Alternate gene names: 78066108

Gene position: 1619405-1621171 (Clockwise)

Preceding gene: 78066107

Following gene: 78066109

Centisome position: 43.84

GC content: 71.53

Gene sequence:

>1767_bases
TTGACAGATATCCACGATATTGAATCGTCCGCGCCTGCCGTGCAGGCAGCGCGTGCCGACGATGCACCGGAGCAGGGCGC
TTCGGCCGAGGGTGGCGACGAGCGTCCCCGCCGCGGTCTGCGGCGAGGCCCGCGCAGCCTGATCGCGCGGCGTCGCGCGG
CGGCCAAGTCGAAGGGCGCCGAAGGCGAGCCGCAGGGCGGCGAAGGCGCTGACGCGCAGCCGGCCGAAGCCGCCGAAGCG
CAGCAGCCGACGCGTGCGCCGCGCAAGGAAGGTGCGGCCAAGGGTGCTCGCAAGCCGGCCGCCAAGCGCGAAGGCGCGGC
CAAGGCCCCGGGCGGCCAAGGCGGCCAGAGTCGTCGCAGCAGCGCACCGAAGGCTGAAGGTGGTGCGGCAAAGGTGGAGG
GCGATGCGTCCCAGGACGAACTGTTCGCCTACGTGACGTCGCCGGCATTCGACGCGGACAACTCCGCGGGCGGTAGCGGC
GTGCGTGCGCCGATGCTGCGCCGCGGTCGCAGCCAGCCGGCGAACAAGCGCGTGCTGTCGCCGGACGACGACGCGCCGAA
GCTTCACAAGGTGCTGGCGGAAGCGGGCATGGGCTCGCGCCGCGAAATGGAAGAGCTGATCGTCGCCGGCCGCGTGTCGG
TGAACGGTGAGCCCGCGCACATCGGCCAGCGCATCATGCCGACCGACCAGGTGCGGATCAACGGCAAGCCGGTCAAGCGC
AAGCTGCCGAACAAGCCGCCGCGCGTGCTGCTGTACCACAAGCCGACGGGCGAGATCGTCAGCCATGCGGATCCGGAAGG
TCGTCCGTCGGTGTTCGACCGCCTGCCGCCGATGAAGACGGCGAAATGGCTCGCGGTGGGGCGCCTCGATTTCAATACCG
AAGGTCTGCTGATGCTGACGACTTCGGGCGACCTCGCAAACCGCTTCATGCATCCGCGCTACAGCGTCGAGCGCGAGTAC
GCGGTGCGCGTCGTCGGCGAACTGGCCGAAGGCATGCGGCAGAAGCTGCTGCACGGCGTCGAGCTCGATGACGGCCCGGC
GAACTTCCTGCGCATCCGCGACGGCGGCGGCGAAGGCACGAACCACTGGTATCACGTTGCGCTGGCCGAAGGCCGCAACC
GCGAAGTGCGCCGGATGTTCGAGGCGGTCGGCCTGATGGTGAGCCGCCTGATCCGTACGCGTCACGGCCCGATTCCGCTG
CCGCGCGGCCTGAAGCGCGGTCGCTGGGAGGAGCTCGACGACACGCAGGTGCGCAAGCTGATGGCGACCGTCGGCCTGAA
GGCGCCGTCCGAGGAGAAGGGCAAGCGCGGCGGTACCGGCCAGGCCGAGCGTCGCCAGCCCGATCCGATGCAGACGTCGA
TGGGCTTCATCGGCCGTGAGCCGGTGCTGACGGCGCACGGGCAGCTGGATCAGCCGCGTCGTGGCAGTGGTCGTCGCGGC
GGGCCGGGTCTGCCGGGTCTGAGCGGCTACGGCAGCCTGCCGGTCGCGCCGTCGGGCTTTGGCAATCGCGCTGGCGGCGG
CCGGGATGGCAACCGTGCGGGCGGAGGCGGGCGCGACGGCAATCGCGCCGGCGGTGGCCGTGGCGATGTCGACGGCAACC
GCGCGTCGTACGGCGCAGGCCCCAAGCGTGATGGCGCGGGCCCCAAGCGCGGCGGCAGCAAGGGCGGTAATCGCAACCCG
AACGGCAATCGTGCCGAGGGCGCCGGTAACAGCGGCGGCCCGCGCGGTGGTCAGCGTCCGCAGCGCAGTCGCACGCGCAG
CCGCTGA

Upstream 100 bases:

>100_bases
GCCGGACGACGAAGCAGAACCTGAACAGCGTCGCGCCTGATCGCGGCCAACTTCTTGCCGCGCCCGCGCCGGGCGCGAGA
CCTGACATTTTGAGGTTGTT

Downstream 100 bases:

>100_bases
GCATTCAGGCGAGCGGCGCAAGGCCTCCGGCGTGCCGGCCGAATCGGCATCCGGCTGGCCTTGTCGGCGCCGGGCATTGC
ATTTATTGCTTTCGACTGTA

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MTDIHDIESSAPAVQAARADDAPEQGASAEGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEPQGGEGADAQPAEAAEA
QQPTRAPRKEGAAKGARKPAAKREGAAKAPGGQGGQSRRSSAPKAEGGAAKVEGDASQDELFAYVTSPAFDADNSAGGSG
VRAPMLRRGRSQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKR
KLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREY
AVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPL
PRGLKRGRWEELDDTQVRKLMATVGLKAPSEEKGKRGGTGQAERRQPDPMQTSMGFIGREPVLTAHGQLDQPRRGSGRRG
GPGLPGLSGYGSLPVAPSGFGNRAGGGRDGNRAGGGGRDGNRAGGGRGDVDGNRASYGAGPKRDGAGPKRGGSKGGNRNP
NGNRAEGAGNSGGPRGGQRPQRSRTRSR

Sequences:

>Translated_588_residues
MTDIHDIESSAPAVQAARADDAPEQGASAEGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEPQGGEGADAQPAEAAEA
QQPTRAPRKEGAAKGARKPAAKREGAAKAPGGQGGQSRRSSAPKAEGGAAKVEGDASQDELFAYVTSPAFDADNSAGGSG
VRAPMLRRGRSQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKR
KLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREY
AVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPL
PRGLKRGRWEELDDTQVRKLMATVGLKAPSEEKGKRGGTGQAERRQPDPMQTSMGFIGREPVLTAHGQLDQPRRGSGRRG
GPGLPGLSGYGSLPVAPSGFGNRAGGGRDGNRAGGGGRDGNRAGGGRGDVDGNRASYGAGPKRDGAGPKRGGSKGGNRNP
NGNRAEGAGNSGGPRGGQRPQRSRTRSR
>Mature_587_residues
TDIHDIESSAPAVQAARADDAPEQGASAEGGDERPRRGLRRGPRSLIARRRAAAKSKGAEGEPQGGEGADAQPAEAAEAQ
QPTRAPRKEGAAKGARKPAAKREGAAKAPGGQGGQSRRSSAPKAEGGAAKVEGDASQDELFAYVTSPAFDADNSAGGSGV
RAPMLRRGRSQPANKRVLSPDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKRK
LPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLTTSGDLANRFMHPRYSVEREYA
VRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGTNHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLP
RGLKRGRWEELDDTQVRKLMATVGLKAPSEEKGKRGGTGQAERRQPDPMQTSMGFIGREPVLTAHGQLDQPRRGSGRRGG
PGLPGLSGYGSLPVAPSGFGNRAGGGRDGNRAGGGGRDGNRAGGGRGDVDGNRASYGAGPKRDGAGPKRGGSKGGNRNPN
GNRAEGAGNSGGPRGGQRPQRSRTRSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=245, Percent_Identity=56.734693877551, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=30.327868852459, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=34.8958333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788510, Length=218, Percent_Identity=29.8165137614679, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 62216; Mature: 62084

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIHDIESSAPAVQAARADDAPEQGASAEGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGEPQGGEGADAQPAEAAEAQQPTRAPRKEGAAKGARKPAAKREGAAKAPGGQGGQSRRS
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHC
SAPKAEGGAAKVEGDASQDELFAYVTSPAFDADNSAGGSGVRAPMLRRGRSQPANKRVLS
CCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
PDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKR
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHH
KLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLT
HCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEE
TSGDLANRFMHPRYSVEREYAVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGT
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
NHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDDTQVRKL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
MATVGLKAPSEEKGKRGGTGQAERRQPDPMQTSMGFIGREPVLTAHGQLDQPRRGSGRRG
HHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
GPGLPGLSGYGSLPVAPSGFGNRAGGGRDGNRAGGGGRDGNRAGGGRGDVDGNRASYGAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PKRDGAGPKRGGSKGGNRNPNGNRAEGAGNSGGPRGGQRPQRSRTRSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TDIHDIESSAPAVQAARADDAPEQGASAEGGDERPRRGLRRGPRSLIARRRAAAKSKGA
CCCHHHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCC
EGEPQGGEGADAQPAEAAEAQQPTRAPRKEGAAKGARKPAAKREGAAKAPGGQGGQSRRS
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCHHHC
SAPKAEGGAAKVEGDASQDELFAYVTSPAFDADNSAGGSGVRAPMLRRGRSQPANKRVLS
CCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCC
PDDDAPKLHKVLAEAGMGSRREMEELIVAGRVSVNGEPAHIGQRIMPTDQVRINGKPVKR
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCHHHHCCCCCCCCEEEECCCCHHH
KLPNKPPRVLLYHKPTGEIVSHADPEGRPSVFDRLPPMKTAKWLAVGRLDFNTEGLLMLT
HCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHHCCCCCCCHHEEEEECEEECCCCEEEEE
TSGDLANRFMHPRYSVEREYAVRVVGELAEGMRQKLLHGVELDDGPANFLRIRDGGGEGT
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
NHWYHVALAEGRNREVRRMFEAVGLMVSRLIRTRHGPIPLPRGLKRGRWEELDDTQVRKL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
MATVGLKAPSEEKGKRGGTGQAERRQPDPMQTSMGFIGREPVLTAHGQLDQPRRGSGRRG
HHHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCC
GPGLPGLSGYGSLPVAPSGFGNRAGGGRDGNRAGGGGRDGNRAGGGRGDVDGNRASYGAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PKRDGAGPKRGGSKGGNRNPNGNRAEGAGNSGGPRGGQRPQRSRTRSR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]