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The map label for this gene is ramA [H]

Identifier: 78065984

GI number: 78065984

Start: 1478072

End: 1478893

Strand: Reverse

Name: ramA [H]

Synonym: Bcep18194_A4513

Alternate gene names: 78065984

Gene position: 1478893-1478072 (Counterclockwise)

Preceding gene: 78065985

Following gene: 78065980

Centisome position: 40.03

GC content: 66.3

Gene sequence:

>822_bases
ATGAAACTGAAGCTCGATATCGTCCAGCTCGCCGGTCGCGACGGCGACACGCACTACAACCTGCAACGCACGCTCGAAGC
GATCGCAACCTGCGCACCGGGCACCGACATCGTGATGTTTCCGGAGGCGCAGCTCACCGGCTTCCTCGATCCGTCGAATC
TGGCCGAATGCGCCGAGCCGCTCGACGGCCCGAGCGTCGGCGCGGTCATTACGGCCGCGCGGGCACGCGATGTGGCGGTC
GTGGTCGGGCTGATCGAGAACGACGGCGGCCGCTTCTACAACACCACCGTGTTCGTCACGCCGGACGGCATCGCGCTGCG
CTATCGCAAGACACATCTGTGGGTGAGCGAGCACGGGGTCGTGCTGCCCGGCGACCGTTACGCGACGATCGAATGGCGCG
GCGTGCGGATCGGCCTGCTGATCTGCTACGACAACGAATTCCCCGAAACCGGCCGCGCGCTCGCCGCGCTCGGCGCCGAG
CTGATCCTCATCACCGACGGCAACATGGAGCCGTACCGCCCTGTCCACCGCACGTCGGCCACAGCGCGGGCGATGGAGAA
CCAGGTGTTCGCGGTGGTCGCCAACCGCGTCGGCGGTAGCACACACGACGTCGTGTTCGCAGGCGGCAGCCTTGCCGCCG
ATCCGTTCGGCAACCTGATCTTCGAAGCCGGCAATACCGAATCGCGTCATGCGGTCGAACTCGATTTCGACCAGCTCGCC
GCGTCGCGTGCGGTCTACGACTATCGCCGGGATCAGCGCCTGCACATCGCCGGCGAACGCATCGAGCATGCCAACGGCCT
GCGCGAACTGCTGATTCCGTAA

Upstream 100 bases:

>100_bases
GCCGGCGGGATGGGAAATCCGCCGCTTCGGCGCATACTGGGGCCTCCTTACCCACCCTTCCGGCGCCACCGCGCGACGGG
CCCCCGCTACCGAAATCGCC

Downstream 100 bases:

>100_bases
GCGCTTGCGCTCCCCCGGCAGTGACATCCGTTATGCCGGGTCCGGCACCTCGATCGCCAGCAGCGCCGAACTGACCGACT
TGCCGTGCGCGTCGAGCGCG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKLKLDIVQLAGRDGDTHYNLQRTLEAIATCAPGTDIVMFPEAQLTGFLDPSNLAECAEPLDGPSVGAVITAARARDVAV
VVGLIENDGGRFYNTTVFVTPDGIALRYRKTHLWVSEHGVVLPGDRYATIEWRGVRIGLLICYDNEFPETGRALAALGAE
LILITDGNMEPYRPVHRTSATARAMENQVFAVVANRVGGSTHDVVFAGGSLAADPFGNLIFEAGNTESRHAVELDFDQLA
ASRAVYDYRRDQRLHIAGERIEHANGLRELLIP

Sequences:

>Translated_273_residues
MKLKLDIVQLAGRDGDTHYNLQRTLEAIATCAPGTDIVMFPEAQLTGFLDPSNLAECAEPLDGPSVGAVITAARARDVAV
VVGLIENDGGRFYNTTVFVTPDGIALRYRKTHLWVSEHGVVLPGDRYATIEWRGVRIGLLICYDNEFPETGRALAALGAE
LILITDGNMEPYRPVHRTSATARAMENQVFAVVANRVGGSTHDVVFAGGSLAADPFGNLIFEAGNTESRHAVELDFDQLA
ASRAVYDYRRDQRLHIAGERIEHANGLRELLIP
>Mature_273_residues
MKLKLDIVQLAGRDGDTHYNLQRTLEAIATCAPGTDIVMFPEAQLTGFLDPSNLAECAEPLDGPSVGAVITAARARDVAV
VVGLIENDGGRFYNTTVFVTPDGIALRYRKTHLWVSEHGVVLPGDRYATIEWRGVRIGLLICYDNEFPETGRALAALGAE
LILITDGNMEPYRPVHRTSATARAMENQVFAVVANRVGGSTHDVVFAGGSLAADPFGNLIFEAGNTESRHAVELDFDQLA
ASRAVYDYRRDQRLHIAGERIEHANGLRELLIP

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=269, Percent_Identity=28.6245353159851, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI297632348, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI297632350, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI5031947, Length=238, Percent_Identity=26.4705882352941, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1786412, Length=258, Percent_Identity=29.4573643410853, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=284, Percent_Identity=26.4084507042254, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 29725; Mature: 29725

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLDIVQLAGRDGDTHYNLQRTLEAIATCAPGTDIVMFPEAQLTGFLDPSNLAECAEP
CEEEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCEEEECCHHEEECCCCCHHHHHHCC
LDGPSVGAVITAARARDVAVVVGLIENDGGRFYNTTVFVTPDGIALRYRKTHLWVSEHGV
CCCCCCCCEEEECCCCCEEEEEEEEECCCCEEEEEEEEECCCCEEEEEEEEEEEEECCCE
VLPGDRYATIEWRGVRIGLLICYDNEFPETGRALAALGAELILITDGNMEPYRPVHRTSA
ECCCCCEEEEEEECEEEEEEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCHHHCHH
TARAMENQVFAVVANRVGGSTHDVVFAGGSLAADPFGNLIFEAGNTESRHAVELDFDQLA
HHHHHHCCEEEEEEHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECHHHHH
ASRAVYDYRRDQRLHIAGERIEHANGLRELLIP
HHHHHHHHHCCCEEEEECHHHHHHCCHHHHCCC
>Mature Secondary Structure
MKLKLDIVQLAGRDGDTHYNLQRTLEAIATCAPGTDIVMFPEAQLTGFLDPSNLAECAEP
CEEEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCEEEECCHHEEECCCCCHHHHHHCC
LDGPSVGAVITAARARDVAVVVGLIENDGGRFYNTTVFVTPDGIALRYRKTHLWVSEHGV
CCCCCCCCEEEECCCCCEEEEEEEEECCCCEEEEEEEEECCCCEEEEEEEEEEEEECCCE
VLPGDRYATIEWRGVRIGLLICYDNEFPETGRALAALGAELILITDGNMEPYRPVHRTSA
ECCCCCEEEEEEECEEEEEEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCHHHCHH
TARAMENQVFAVVANRVGGSTHDVVFAGGSLAADPFGNLIFEAGNTESRHAVELDFDQLA
HHHHHHCCEEEEEEHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEECHHHHH
ASRAVYDYRRDQRLHIAGERIEHANGLRELLIP
HHHHHHHHHCCCEEEEECHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA