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The map label for this gene is bkdB [H]

Identifier: 78065833

GI number: 78065833

Start: 1317420

End: 1318757

Strand: Direct

Name: bkdB [H]

Synonym: Bcep18194_A4362

Alternate gene names: 78065833

Gene position: 1317420-1318757 (Clockwise)

Preceding gene: 78065832

Following gene: 78065834

Centisome position: 35.66

GC content: 68.61

Gene sequence:

>1338_bases
ATGGGGATTCATGTCATCAAGATGCCGGATATCGGCGAAGGGATTGCCGAGGTCGAGCTGGTGGCCTGGCACGTGGAAGT
CGGGCAGACGATCAAGGAAGACCAGCCGCTCGCCGATGTGATGACCGACAAGGCGGCGGTCGAGATTCCGTCGCCGGTGA
CGGGCAAGGTGATCGAACTCGGCGGCCGGATCGGCGAGATGATGGCGGTCGGCAGCGAGCTGATTCGCCTTGAGGTCGAA
GGCGACGGCAACCTGAAGGCCGGCGCACCGGTGCGGGAATCAAAGGTAGCAACCGCACCGGTCGCGGCAGCGCCGTCGAA
GGCGGTGGCCGACGCGGCGGCGGAATCGTCCGCCAAGCCGGCGCCGAAGCACGCACCGGCGGAGCCGCGTCGCGCGAAGC
ATGCCGAGCACGCGGAGCGCGTCGAGCCGCCGCGCGCGGCGCTCGCGCCGGGTGAACGGCCGCTCGCGTCGCCGGCCGTG
CGCCAGCGCGCGTGGGACATGGGCGTCGAGCTGCGCTACGTGCGCGGCACCGGTGAAGCAGGGCGGATCCTGCACGCGGA
TCTCGATGCGTATGCAGGATCCGGCAGCGGGGCGGCGCGCGGATCGCGTGGCGCGCATGCGCACGGCTACGGCGAACGCA
ACGATGAAACCGAAGTGCCGGTGATCGGCTTGCGGCGAGCGATCGCACGCAAGATGCAGGAAGCGAAGCGCCGCATTCCG
CACTTCAGCTACGTCGAGGAGATCGACGTCACCGAGCTCGAATCGCTGCGTACGGATCTGAACCGCCGCTACGGCGACAC
GCGCGGCAAGCTCACGCCGCTGCCGTTGCTGATCCGCGCGATGGTGATCGCGTTGCGCGACTTTCCGCAGATCAACGCGC
GTTTCGACGACGAAGCGGGTGTCGTCACGCGCTACGGCGCGGTGCACATGGGCGTCGCGACGCAGACGGACGGCGGCCTC
ACGGTGCCCGTGCTGCGTCATGCCGAAGCGCGCGACGTGTGGTCGATTTCCGCCGAAATCGCGCGGCTCGCCGACGCAGT
GCGCGCGAACCGTGCGCAGCGCGACGAGCTGAGCGGCTCGACGATCACGATCTCGAGCCTCGGCGCGCTCGGCGGCATCG
TGTCGACACCGGTCATCAATCATCCTGAAGTCGGCATCGTCGGCGTGAACCGCATCGTCGAGCGGCCGATGATCCGCGAC
GGCGCGGTCGTCGCACGCAAGATGATGAACCTGTCGTCGTCGTTCGACCATCGCGTCGTCGATGGCGCGGACGCAGCCGA
ATTCATCCAGGCCGTGCGCGCGGTGCTCGAGCGCCCGGCACTGCTGTTCGTGGAGTGA

Upstream 100 bases:

>100_bases
ACGGGCTGGGATACGCCGTATCCGCATGCGCAGGAGTGGGCGTACTTTCCGGGCCCGGCCCGGGTCGGTGAAGCGTTGCG
ACGCGTGATGGAGGCGTGAG

Downstream 100 bases:

>100_bases
GCGCGATGAAGAATGAACATACCACGCTGCTCGTGATTGGCGGCGGCCCGGGCGGCTACGTCGCCGCGATTCGCGCCGGC
CAGCTCGGCATTCCGACCGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVE
GDGNLKAGAPVRESKVATAPVAAAPSKAVADAAAESSAKPAPKHAPAEPRRAKHAEHAERVEPPRAALAPGERPLASPAV
RQRAWDMGVELRYVRGTGEAGRILHADLDAYAGSGSGAARGSRGAHAHGYGERNDETEVPVIGLRRAIARKMQEAKRRIP
HFSYVEEIDVTELESLRTDLNRRYGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGL
TVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRD
GAVVARKMMNLSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE

Sequences:

>Translated_445_residues
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVE
GDGNLKAGAPVRESKVATAPVAAAPSKAVADAAAESSAKPAPKHAPAEPRRAKHAEHAERVEPPRAALAPGERPLASPAV
RQRAWDMGVELRYVRGTGEAGRILHADLDAYAGSGSGAARGSRGAHAHGYGERNDETEVPVIGLRRAIARKMQEAKRRIP
HFSYVEEIDVTELESLRTDLNRRYGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGL
TVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRD
GAVVARKMMNLSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE
>Mature_444_residues
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIELGGRIGEMMAVGSELIRLEVEG
DGNLKAGAPVRESKVATAPVAAAPSKAVADAAAESSAKPAPKHAPAEPRRAKHAEHAERVEPPRAALAPGERPLASPAVR
QRAWDMGVELRYVRGTGEAGRILHADLDAYAGSGSGAARGSRGAHAHGYGERNDETEVPVIGLRRAIARKMQEAKRRIPH
FSYVEEIDVTELESLRTDLNRRYGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAGVVTRYGAVHMGVATQTDGGLT
VPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGSTITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDG
AVVARKMMNLSSSFDHRVVDGADAAEFIQAVRAVLERPALLFVE

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=465, Percent_Identity=29.4623655913978, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI31711992, Length=446, Percent_Identity=26.457399103139, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=29.6, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI203098816, Length=233, Percent_Identity=28.3261802575107, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI203098753, Length=233, Percent_Identity=28.3261802575107, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=28.9308176100629, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786305, Length=296, Percent_Identity=31.0810810810811, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786946, Length=450, Percent_Identity=26.6666666666667, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=456, Percent_Identity=31.5789473684211, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=26.530612244898, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=26.5033407572383, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=25.08038585209, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=443, Percent_Identity=27.0880361173815, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=26.6233766233766, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI18859875, Length=462, Percent_Identity=29.004329004329, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=31.6017316017316, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=30.9734513274336, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=30.9734513274336, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47601; Mature: 47470

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIEL
CCEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHCCCCEEECCCCCCCHHHHH
GGRIGEMMAVGSELIRLEVEGDGNLKAGAPVRESKVATAPVAAAPSKAVADAAAESSAKP
HHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
APKHAPAEPRRAKHAEHAERVEPPRAALAPGERPLASPAVRQRAWDMGVELRYVRGTGEA
CCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
GRILHADLDAYAGSGSGAARGSRGAHAHGYGERNDETEVPVIGLRRAIARKMQEAKRRIP
CCEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HFSYVEEIDVTELESLRTDLNRRYGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAG
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTRYGAVHMGVATQTDGGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGS
EEEECCEEEECEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
TITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMMNLSSSFDHRVV
EEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DGADAAEFIQAVRAVLERPALLFVE
CCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
GIHVIKMPDIGEGIAEVELVAWHVEVGQTIKEDQPLADVMTDKAAVEIPSPVTGKVIEL
CEEEEECCCCCCCHHHEEEEEEEEHHCCHHHCCCCHHHHHCCCCEEECCCCCCCHHHHH
GGRIGEMMAVGSELIRLEVEGDGNLKAGAPVRESKVATAPVAAAPSKAVADAAAESSAKP
HHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
APKHAPAEPRRAKHAEHAERVEPPRAALAPGERPLASPAVRQRAWDMGVELRYVRGTGEA
CCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCC
GRILHADLDAYAGSGSGAARGSRGAHAHGYGERNDETEVPVIGLRRAIARKMQEAKRRIP
CCEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
HFSYVEEIDVTELESLRTDLNRRYGDTRGKLTPLPLLIRAMVIALRDFPQINARFDDEAG
CCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VVTRYGAVHMGVATQTDGGLTVPVLRHAEARDVWSISAEIARLADAVRANRAQRDELSGS
EEEECCEEEECEEEECCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
TITISSLGALGGIVSTPVINHPEVGIVGVNRIVERPMIRDGAVVARKMMNLSSSFDHRVV
EEEEEHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
DGADAAEFIQAVRAVLERPALLFVE
CCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]