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The map label for this gene is lpdV [H]

Identifier: 78065834

GI number: 78065834

Start: 1318763

End: 1320154

Strand: Direct

Name: lpdV [H]

Synonym: Bcep18194_A4363

Alternate gene names: 78065834

Gene position: 1318763-1320154 (Clockwise)

Preceding gene: 78065833

Following gene: 78065836

Centisome position: 35.7

GC content: 71.34

Gene sequence:

>1392_bases
ATGAAGAATGAACATACCACGCTGCTCGTGATTGGCGGCGGCCCGGGCGGCTACGTCGCCGCGATTCGCGCCGGCCAGCT
CGGCATTCCGACCGTGCTCGTCGAGCGCGACCGGCTCGGCGGCACGTGCCTGAACATCGGCTGCATTCCGTCGAAGGCGC
TGATCCACGTGGCCGACGCATTCGAACAGGCCCGCGGCCACGCGGGCGAGGGCGCGCTCGGAATCCGCGTGCGCACGCCC
GAGATCGACATCGGGAAAAGCGTCGCCTGGAAGGACGGCATCGTCGACCGGCTGACGCGCGGGGTCGGCGCGCTGCTCAA
GAAGAACGGCGTGCGCGTGCTGCACGGCGACGCGCAGGTGGTCGACGGCAAGACCGTCGACGTCGTCGCCGGCGGCCACA
CGACGCGAATCAGCTGCGAGCACCTGTTGCTCGCGACCGGCTCCGAACCGGTCGCGCTGCCGTCGATGCCGTTCGGCGGG
CATGTCGTGTCGTCCACCGAGGCGCTGTCGCCCGAGACGTTGCCGAAGCGGCTGGTCGTCGTCGGGGCCGGGTATATCGG
GCTCGAACTCGGGATCGTCTATCGCAAGCTCGGCGTCGACGTCAGCGTCGTCGAAGCGGCGGAGCGCGTGTTGCCCGCGT
ACGATGCCGAACTCGTGCGGCCGGTCGCCGATTCGCTCGCGCGGCTCGGCGTGCGGCTGTTGCTCGGCCACAAGGTGCTC
GGGCCCGCGGAACACGGCGCGGTGCGCGTGCAGGCCGCGGATGGCGCGGAGCAGACGCTGCAGGCCGATCGCGTGCTGGT
TGCGGTCGGCCGCCGGCCGCGGGTCGACGGCTTCGGGCTCGAGTCGCTGCCGCTCGATCGCAACGGTCGCGCGCTGTGGA
TCGACGACGAATGCCGGACGTCGATGCGCAACGTCTGGGCGATCGGCGACGTGGCCGGCGAGCCGATGCTCGCGCATCGC
GCGATGGCGCAGGGCGAGATGGTGGCCGAGCTGATCGCCGGCAAGCGCCGCAAGTTCATGCCCGCGTCGATCCCGGCCGT
GTGCTTCACCGACCCGGAGATCGTCACGGCCGGCTGGTCGCCGGATGACGCGCGTGCGGCCGATGTCGATTGCGTGAGCG
CGTCGTTCCCGTTCGCGGCGAACGGGCGCGCGATGACGCTGCAGGCGACCGACGGCTTCGTGCGCGTCGTCGCGCGACGC
GACAACCACCTGATCGTCGGCTGGCAGGCGGTCGGGCGCGGCGTGTCGGAGCTGGCCGCGGCGTTCTCGCAGTCGCTGGA
GATGGGCGCGCGACTGGAGGACATCGGCGGCACGATCCATGCGCACCCGACGCTCGGCGAAGCACTGCAGGAAGCCGCGC
TGCGTGCGCTCGGGCACGCGTTGCACGTCTGA

Upstream 100 bases:

>100_bases
TCGACCATCGCGTCGTCGATGGCGCGGACGCAGCCGAATTCATCCAGGCCGTGCGCGCGGTGCTCGAGCGCCCGGCACTG
CTGTTCGTGGAGTGAGCGCG

Downstream 100 bases:

>100_bases
CGTGCAGCACGCGTGACACAACGGGCCGCACCGATAGGCGATGCGGGTATCGGTGCGGCTCCGTGCATTTCCCGCCTGAC
AGGTGGCGGGAGATGCCGTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQARGHAGEGALGIRVRTP
EIDIGKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGDAQVVDGKTVDVVAGGHTTRISCEHLLLATGSEPVALPSMPFGG
HVVSSTEALSPETLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLLLGHKVL
GPAEHGAVRVQAADGAEQTLQADRVLVAVGRRPRVDGFGLESLPLDRNGRALWIDDECRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGKRRKFMPASIPAVCFTDPEIVTAGWSPDDARAADVDCVSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV

Sequences:

>Translated_463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQARGHAGEGALGIRVRTP
EIDIGKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGDAQVVDGKTVDVVAGGHTTRISCEHLLLATGSEPVALPSMPFGG
HVVSSTEALSPETLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLLLGHKVL
GPAEHGAVRVQAADGAEQTLQADRVLVAVGRRPRVDGFGLESLPLDRNGRALWIDDECRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGKRRKFMPASIPAVCFTDPEIVTAGWSPDDARAADVDCVSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
>Mature_463_residues
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADAFEQARGHAGEGALGIRVRTP
EIDIGKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGDAQVVDGKTVDVVAGGHTTRISCEHLLLATGSEPVALPSMPFGG
HVVSSTEALSPETLPKRLVVVGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLLLGHKVL
GPAEHGAVRVQAADGAEQTLQADRVLVAVGRRPRVDGFGLESLPLDRNGRALWIDDECRTSMRNVWAIGDVAGEPMLAHR
AMAQGEMVAELIAGKRRKFMPASIPAVCFTDPEIVTAGWSPDDARAADVDCVSASFPFAANGRAMTLQATDGFVRVVARR
DNHLIVGWQAVGRGVSELAAAFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=37.8260869565217, Blast_Score=275, Evalue=1e-73,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=29.0598290598291, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI22035672, Length=434, Percent_Identity=27.6497695852535, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI33519430, Length=438, Percent_Identity=23.972602739726, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519428, Length=438, Percent_Identity=23.972602739726, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI33519426, Length=438, Percent_Identity=23.972602739726, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277065, Length=438, Percent_Identity=23.7442922374429, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277071, Length=438, Percent_Identity=23.972602739726, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=23.6781609195402, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI291045268, Length=429, Percent_Identity=23.0769230769231, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786307, Length=455, Percent_Identity=37.3626373626374, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=29.0322580645161, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=26.9396551724138, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=25.7206208425721, Blast_Score=128, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=39.5196506550218, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=26.9406392694064, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=438, Percent_Identity=26.9406392694064, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=24.0165631469979, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=37.2384937238494, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=29.6137339055794, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=26.215644820296, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=38.9978213507625, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=25.7383966244726, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=25.9958071278826, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640553, Length=474, Percent_Identity=25.7383966244726, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640551, Length=474, Percent_Identity=25.7383966244726, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48655; Mature: 48655

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADA
CCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FEQARGHAGEGALGIRVRTPEIDIGKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGDAQV
HHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCEEEECCCEE
VDGKTVDVVAGGHTTRISCEHLLLATGSEPVALPSMPFGGHVVSSTEALSPETLPKRLVV
ECCCEEEEEECCCEEEEEEEEEEEECCCCCEECCCCCCCCEEECCCCCCCCCCCCCEEEE
VGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLLLGHKVL
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GPAEHGAVRVQAADGAEQTLQADRVLVAVGRRPRVDGFGLESLPLDRNGRALWIDDECRT
CCCCCCEEEEEECCCHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGKRRKFMPASIPAVCFTDPEIVTAGWS
HHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEECCCC
PDDARAADVDCVSASFPFAANGRAMTLQATDGFVRVVARRDNHLIVGWQAVGRGVSELAA
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNEHTTLLVIGGGPGGYVAAIRAGQLGIPTVLVERDRLGGTCLNIGCIPSKALIHVADA
CCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FEQARGHAGEGALGIRVRTPEIDIGKSVAWKDGIVDRLTRGVGALLKKNGVRVLHGDAQV
HHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCEEEECCCEE
VDGKTVDVVAGGHTTRISCEHLLLATGSEPVALPSMPFGGHVVSSTEALSPETLPKRLVV
ECCCEEEEEECCCEEEEEEEEEEEECCCCCEECCCCCCCCEEECCCCCCCCCCCCCEEEE
VGAGYIGLELGIVYRKLGVDVSVVEAAERVLPAYDAELVRPVADSLARLGVRLLLGHKVL
EECCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GPAEHGAVRVQAADGAEQTLQADRVLVAVGRRPRVDGFGLESLPLDRNGRALWIDDECRT
CCCCCCEEEEEECCCHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHH
SMRNVWAIGDVAGEPMLAHRAMAQGEMVAELIAGKRRKFMPASIPAVCFTDPEIVTAGWS
HHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEECCCC
PDDARAADVDCVSASFPFAANGRAMTLQATDGFVRVVARRDNHLIVGWQAVGRGVSELAA
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCEEEEEHHHHCCCHHHHHH
AFSQSLEMGARLEDIGGTIHAHPTLGEALQEAALRALGHALHV
HHHHHHHHCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]