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The map label for this gene is queA

Identifier: 78065287

GI number: 78065287

Start: 707215

End: 708282

Strand: Reverse

Name: queA

Synonym: Bcep18194_A3811

Alternate gene names: 78065287

Gene position: 708282-707215 (Counterclockwise)

Preceding gene: 78065292

Following gene: 78065286

Centisome position: 19.17

GC content: 69.57

Gene sequence:

>1068_bases
ATGTTCACGCTTTCCGATTTCGATTTCAATCTGCCGCCCGAGCTGATTGCCCAAACCGCGCTGCCCGATCGCACCGCGAG
CCGCCTGCTCGAGGTCGACGGCACCGTCGCGCCCGCACGCCTGGTCGACCGCCATTTCACCGAGCTGCCGTCGTGCATCT
CGGCCGGCGACCTGCTCGTCTTCAACGATACCAAGGTGCTGAAGGCGCGCTTCTTCGGCCAGAAGGCCAGCGGCGGCAAG
ATCGAGGTGCTGATCGAGCGCGTGACCGGCACGCACACGGCGCTCGCGCAGATCCGCGCGAGCAAGTCGCCGGGCGCCGG
CACGACGCTGCGGCTCGCCGATGCGTTCGACGTGACGGTCGGCGAGCGCGTCGACCCGTTCTTCACGCTGAACTTCCCCG
AGCCGTGCCTCGACCTGATCGAGCAGTACGGCCGCCTGCCGCTGCCGCCCTATATCGAGCACGATCCGGACGCGACCGAC
GAGACGCGCTACCAGACCGTCTACGCGAGCAATCCGGGCGCGGTCGCCGCGCCGACCGCCGGGCTGCACTTCGACCAGCC
GATGCTCGACCGGCTCGACGCGATGGGCGTCGAGCGCGCGACGCTGACGCTGCACGTCGGCGCCGGCACGTTCCAGCCGG
TGCGCGTCGACAACATCGCCGAGCACAAGATGCACAGCGAGTGGTACGACCTGCCGCAGTCGCTCGTCGACAAGATCGCC
GCGACCCGCGCACGCGGCGGCAACGTGATCGCGGTCGGCACGACGTCGATGCGCGCGCTCGAAGCCGCGGCGCGCGCGGC
CGATGAAGCGGGTCGCCCGCTCGCGGCCACGCAGGCCGAGACCGACATCTTCATCACGCCCGGCTATCGTTTCCGCGTGG
TCGACCGGCTCGTCACGAACTTCCACCTGCCGAAGTCGACGCTGCTGATGCTGGTGTCCGCGTTCGCGGGCGTCGAGACG
ATCCGCGCCGCGTACCGCCACGCGATCGAGCAGCGCTACCGCTTCTTCAGCTACGGCGATGCGATGCTGCTGACGCGGCG
CGACACGCCGGAGGCGCCGCTCGCGTAA

Upstream 100 bases:

>100_bases
CGGCCAGGCCGCGCATCCCGTCATCATAGCGGCTCGCGCGCCCCATTTACGCGTTTCCGCCCTGCCGGCGACGCCCGCCG
GCCGCTTCGAACCAGCCCGC

Downstream 100 bases:

>100_bases
GCGCGGCATGCGCGACGGCCCGCGCACCGCGCGGCCACCGTGCCCCGTTTTCCCGCTGCGCGCGCTACGATGGCGCGCAC
CCGATTTTTCCCCGCCGCCG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGTVAPARLVDRHFTELPSCISAGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVDPFFTLNFPEPCLDLIEQYGRLPLPPYIEHDPDATD
ETRYQTVYASNPGAVAAPTAGLHFDQPMLDRLDAMGVERATLTLHVGAGTFQPVRVDNIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARAADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEAPLA

Sequences:

>Translated_355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGTVAPARLVDRHFTELPSCISAGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVDPFFTLNFPEPCLDLIEQYGRLPLPPYIEHDPDATD
ETRYQTVYASNPGAVAAPTAGLHFDQPMLDRLDAMGVERATLTLHVGAGTFQPVRVDNIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARAADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEAPLA
>Mature_355_residues
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGTVAPARLVDRHFTELPSCISAGDLLVFNDTKVLKARFFGQKASGGK
IEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTVGERVDPFFTLNFPEPCLDLIEQYGRLPLPPYIEHDPDATD
ETRYQTVYASNPGAVAAPTAGLHFDQPMLDRLDAMGVERATLTLHVGAGTFQPVRVDNIAEHKMHSEWYDLPQSLVDKIA
ATRARGGNVIAVGTTSMRALEAAARAADEAGRPLAATQAETDIFITPGYRFRVVDRLVTNFHLPKSTLLMLVSAFAGVET
IRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEAPLA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=53.314121037464, Blast_Score=353, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38900; Mature: 38900

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGTVAPARLVDRHFTELPSCISAGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
FNDTKVLKARFFGQKASGGKIEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCEECCC
GERVDPFFTLNFPEPCLDLIEQYGRLPLPPYIEHDPDATDETRYQTVYASNPGAVAAPTA
CCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDQPMLDRLDAMGVERATLTLHVGAGTFQPVRVDNIAEHKMHSEWYDLPQSLVDKIA
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
ATRARGGNVIAVGTTSMRALEAAARAADEAGRPLAATQAETDIFITPGYRFRVVDRLVTN
HHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGVETIRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEAPLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MFTLSDFDFNLPPELIAQTALPDRTASRLLEVDGTVAPARLVDRHFTELPSCISAGDLLV
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
FNDTKVLKARFFGQKASGGKIEVLIERVTGTHTALAQIRASKSPGAGTTLRLADAFDVTV
ECCCHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCEECCC
GERVDPFFTLNFPEPCLDLIEQYGRLPLPPYIEHDPDATDETRYQTVYASNPGAVAAPTA
CCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCC
GLHFDQPMLDRLDAMGVERATLTLHVGAGTFQPVRVDNIAEHKMHSEWYDLPQSLVDKIA
CCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHH
ATRARGGNVIAVGTTSMRALEAAARAADEAGRPLAATQAETDIFITPGYRFRVVDRLVTN
HHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCCHHHHHHHHHHH
FHLPKSTLLMLVSAFAGVETIRAAYRHAIEQRYRFFSYGDAMLLTRRDTPEAPLA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA