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The map label for this gene is tgt

Identifier: 78065286

GI number: 78065286

Start: 705866

End: 707053

Strand: Reverse

Name: tgt

Synonym: Bcep18194_A3810

Alternate gene names: 78065286

Gene position: 707053-705866 (Counterclockwise)

Preceding gene: 78065287

Following gene: 78065285

Centisome position: 19.14

GC content: 66.16

Gene sequence:

>1188_bases
ATGACCGAAGGTTCATCCCACGATACGCACGCCCCCGTGCAGGACCGCCCGGCGAACGGGCTCGAATTCGAACTGCTGAC
GACCGATGGCCATGCGCGCCGCGGCCGCGTGAAGCTCAATCACGGCGTGGTCGAGACGCCGATCTTCATGCCGGTCGGCA
CGTACGGCACCGTGAAGGCGATCCAGCCGCGCGAGCTGCACGAGATCAAGGCCCAGATCATCCTCGGCAACACGTTCCAC
CTGTGGCTGCGCCCGGGCCTCGACACGATCGACGCGCACGGCGGCCTGCATGCGTTCATGGGCTGGAACAAGCCGATCCT
GACCGACTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGACCTGCGCAAGATCACCGAGGACGGCGTCACGTTCGCGTCGC
CGATCAACGGCGACAAGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGGTGCTGAACTCCGACGTCGTGATG
CAGTTCGACGAGTGCACGCCGTACGCGACCAACGGCGTGCCGACCACGCACAAGGAAGCGGCCGACTCGATGCGCATGTC
GCTGCGCTGGGCGAAGCGCTCGCTCGACGAGTTCAACCGGCTCGGCAACCCGAACGCGCTGTTCGGGATCGTCCAGGGCG
GGATGTTCGAGGACCTGCGCGACGAATCGCTCGCGGGCCTCGCGGAACTCGGCTTCCACGGCCTCGCGATCGGCGGGCTG
TCGGTCGGCGAGCCGAAGGAAGACATGATGCGCGTGCTGCGCCACGTGGGCCCGAAGCTGCCGGCCAACAAGCCGCACTA
CCTGATGGGCGTCGGCACGCCGGAGGACCTGGTCGAAGGCGTCGCCAACGGCGTCGACATGTTCGACTGCGTGATGCCGA
CCCGCAACGCGCGCAACGGCTGGCTGTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAACTCGCTG
AAACCGCTCGACGCGAGCTGCACGTGCTACACGTGCCAGAACTTCTCGCGCGGCTACCTGCATCACCTGCACCGCGTCGG
CGAGATCCTCGGCGCGCAGCTCAACACGATCCACAACCTGCACTACTACCTGCAGCTGATGAGCGAGATCCGCGAAGCGA
TCGAGACCCACACGTTCGACGCGTTCCGCCAGCGCTTCGCGGAGGATCGCGCCAGAGGCGTCGACTGA

Upstream 100 bases:

>100_bases
GCGCTACGATGGCGCGCACCCGATTTTTCCCCGCCGCCGGCTTCCGGCGACGTTTCCAACCTGCGCCGGACTGTTTTCCG
GTAGCGAGGAGAATGACGCG

Downstream 100 bases:

>100_bases
CGGGCCGGCAGGCGCACGGGCGGCCCGATGGGCGGCCCGTCACATGGGACGTGTACTAAATAAAGCGTCCCGACTGTCGA
ACCCGACTGCCAAATATTAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFH
LWLRPGLDTIDAHGGLHAFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVM
QFDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
SVGEPKEDMMRVLRHVGPKLPANKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSL
KPLDASCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIREAIETHTFDAFRQRFAEDRARGVD

Sequences:

>Translated_395_residues
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFH
LWLRPGLDTIDAHGGLHAFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVM
QFDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
SVGEPKEDMMRVLRHVGPKLPANKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSL
KPLDASCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIREAIETHTFDAFRQRFAEDRARGVD
>Mature_394_residues
TEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKAIQPRELHEIKAQIILGNTFHL
WLRPGLDTIDAHGGLHAFMGWNKPILTDSGGFQVFSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQ
FDECTPYATNGVPTTHKEAADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGLS
VGEPKEDMMRVLRHVGPKLPANKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDASCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLQLMSEIREAIETHTFDAFRQRFAEDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=43.943661971831, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=64.1711229946524, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=41.7789757412399, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=164, Percent_Identity=28.6585365853659, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920510, Length=355, Percent_Identity=44.2253521126761, Blast_Score=307, Evalue=9e-84,
Organism=Drosophila melanogaster, GI21355311, Length=166, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43927; Mature: 43795

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEEC
IQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHAFMGWNKPILTDSGGFQVFSLGD
CCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCCCCEEECCCCEEEEECCH
LRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHKEA
HHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
ADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCC
SVGEPKEDMMRVLRHVGPKLPANKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNG
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCC
WLFTRFGDVKIRNATHKNSLKPLDASCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNL
EEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HYYLQLMSEIREAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEGSSHDTHAPVQDRPANGLEFELLTTDGHARRGRVKLNHGVVETPIFMPVGTYGTVKA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEEC
IQPRELHEIKAQIILGNTFHLWLRPGLDTIDAHGGLHAFMGWNKPILTDSGGFQVFSLGD
CCCHHHHHHEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCCCCEEECCCCEEEEECCH
LRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDVVMQFDECTPYATNGVPTTHKEA
HHHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
ADSMRMSLRWAKRSLDEFNRLGNPNALFGIVQGGMFEDLRDESLAGLAELGFHGLAIGGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEECCC
SVGEPKEDMMRVLRHVGPKLPANKPHYLMGVGTPEDLVEGVANGVDMFDCVMPTRNARNG
CCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCC
WLFTRFGDVKIRNATHKNSLKPLDASCTCYTCQNFSRGYLHHLHRVGEILGAQLNTIHNL
EEEEEECCEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HYYLQLMSEIREAIETHTFDAFRQRFAEDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA