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The map label for this gene is prfA

Identifier: 78065073

GI number: 78065073

Start: 469536

End: 470618

Strand: Reverse

Name: prfA

Synonym: Bcep18194_A3597

Alternate gene names: 78065073

Gene position: 470618-469536 (Counterclockwise)

Preceding gene: 78065074

Following gene: 78065072

Centisome position: 12.74

GC content: 65.56

Gene sequence:

>1083_bases
ATGAAGACGAGCATGCAACGCAAGCTCGACCAGCTGTCCACACGGCTGGCCGAACTGAACGACCTGCTGAGCCGCGAAAA
CGTCACGGCCGACCTCGACCAGTACCGCAAGCTCACACGTGAACACGCGGAACTCGGCCCGGTGGTCGAACAGTACGCGC
TGTGGCGCCAGTCGCGCAGCGACGAGACGGCTGCACAGGAACTGCTCGGCGATCTGTCGATGCGCGACTTCGCGGAAGAC
GAAATCCGCAGTGCGCGCGAGAGCATGTCCCGCCTCGAGACCGAGCTGCAGAAGATGCTGCTGCCGAAGGATCCGAACGA
CGAGCGCAACATCTTCCTCGAAATCCGTGCGGGCACGGGCGGCGACGAATCGGCGCTGTTCGCGGGCGACCTGCTGCGCA
TGTACCTGCGCTTCGCGGAACGCCAGCGCTGGCAGGTCGAAATGATGTCGGAAAGTGCGTCGGATCTCGGCGGCTACAAG
GAAGTGATCGTGCGGATCGCGGGCCAGGGCGCGTATTCGCGCCTGAAGTTCGAATCGGGCGGCCATCGCGTGCAGCGCGT
GCCCGCCACCGAAACGCAGGGGCGCATCCACACGTCCGCGTGCACGGTCGCGGTGATGCCGGAAGCCGACGAAATCGGCG
AGGTCGAGATCAATCCGGCCGACCTGCGGATCGACACGTTCCGCGCGTCCGGCGCGGGCGGCCAGCACATCAACAAGACC
GATTCGGCAGTGCGCGTCACGCACATCCCGACCGGGATCGTCGTGGAGTGCCAGGACGACCGTTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAGGTGCTCGCCGCGCGCATCAAGGACAAGCAGTATCACGAGCAGCACGCGAAGGAAGCCGCGACGC
GCAAGAGCCTGATCGGCTCCGGCGACCGCTCGGAACGGATCCGCACGTACAACTTCCCGCAGGGCCGGATGACCGACCAC
CGGATCAACCTGACGCTGTACCGGCTCGAGGCGCTCATGGACGGCGACCTCGACGAACTGATCAGCGCGCTGGTCAGCGA
ACACCAGGCCGAACTGCTCGCGTCGCTCGGCGATGCCGACTGA

Upstream 100 bases:

>100_bases
GACCAGTCCGGCCACTAGCGCCGGCCGCTCGCCCTGCCGCCGGCCGCGTGCCGGCCTATCCTTTCCGAATACCCCTTGCC
CGGGAGCGCCGCTCCACACC

Downstream 100 bases:

>100_bases
GGCCGCGATGCCCGACACCACCGCCGACGAACTGCTGCGCGCGTCGCCGCTCGACGCGGTCGATGCGCGCGTGTTGCTCG
CGCACGCGCTCGGCTGGACC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLGDLSMRDFAED
EIRSARESMSRLETELQKMLLPKDPNDERNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEALMDGDLDELISALVSEHQAELLASLGDAD

Sequences:

>Translated_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLGDLSMRDFAED
EIRSARESMSRLETELQKMLLPKDPNDERNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEALMDGDLDELISALVSEHQAELLASLGDAD
>Mature_360_residues
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRSDETAAQELLGDLSMRDFAED
EIRSARESMSRLETELQKMLLPKDPNDERNIFLEIRAGTGGDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYK
EVIVRIAGQGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEALMDGDLDELISALVSEHQAELLASLGDAD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=273, Percent_Identity=50.5494505494505, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=38.2749326145553, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=48.876404494382, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.4525139664804, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.9221556886228, Blast_Score=220, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=38.7900355871886, Blast_Score=190, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=40.5099150141643, Blast_Score=242, Evalue=6e-65,
Organism=Drosophila melanogaster, GI19921226, Length=266, Percent_Identity=44.3609022556391, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40594; Mature: 40594

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLGDLSMRDFAEDEIRSARESMSRLETELQKMLLPKDPNDERNIFLEIRAGTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEALMDGDLDELISALVSEHQAELLASLGDAD
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQRKLDQLSTRLAELNDLLSRENVTADLDQYRKLTREHAELGPVVEQYALWRQSRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DETAAQELLGDLSMRDFAEDEIRSARESMSRLETELQKMLLPKDPNDERNIFLEIRAGTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRFAERQRWQVEMMSESASDLGGYKEVIVRIAGQGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEALMDGDLDELISALVSEHQAELLASLGDAD
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA